SIRT1 Monoclonal Antibody (1F3)
Invitrogen
catalog: MA5-15677

mouse monoclonal (1F3)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 2

Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 antibody with DAB staining.
quantity: 100 uL
price: US 542.00
to the supplier

SIRT1 Polyclonal Antibody
Invitrogen
catalog: PA5-20964

chicken polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
citations: 2

Western blot analysis of mouse liver tissue lysate using PA5-20964 anti-SIRT1 antibody at 1 ug/mL.

Immunofluorescence of rat lung tissue using PA5-20964 anti-SIRT1 antibody at a concentration of 20 ug/mL.
quantity: 100 ug
price: US 474.00
to the supplier

SIRT1 Polyclonal Antibody
Invitrogen
catalog: PA5-17232

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
citations: 1

Immunoprecipitation of SirT1 was performed on HEK293T cells. Antigen-antibody complexes were formed by incubating 500ug of HEK293T whole cell lysate (in 300ul volume with 5ul of a SirT1 polyclonal antibody (Product # PA5-17232) or a rabbit IgG (negative control) overnight at 4ºC. The immune complexes were captured on 30ul of protein G sepharose beads, washed extensively, and eluted with 6X Laemmli buffer. Samples were resolved on an 8% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with a SirT1 polyclonal antibody (Product # PA5-17232) at a dilution of 1:1000 overnight at 4ºC, washed in TBST, and probed with a peroxidase-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:40,000 for 1 hour at room temperature. Chemiluminescent detection was performed using ECL substrate. Data courtesy of the Innovators Program.

Immunofluorescent analysis of SirT1 (green) in HeLa cells. Cells were fixed in 4% formaldehyde for 15 minutes at room temperature, rinsed with PBS, and blocked with 5% normal goat serum in 1X PBS containing 0.3% Triton X-100 for 1 hour. Cells were probed with a SirT1 polyclonal antibody (Product # PA5-17232) at a dilution of 1:100 overnight at 4ºC, washed with PBS, and incubated with a fluorescently-conjugated anti-rabbit IgG secondary antibody for at least 1 hour at room temperature. F-actin (red) was stained with fluorescently-conjugated Phalloidin. Staining was visualized by confocal microscopy.

Western blot analysis of SirT1 was performed by loading 20ug of THP-1 whole cell lysates per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% milk in TBST for 1 hr at room temperature. The membrane was probed with a SirT1 polyclonal antibody (Product # PA5-17232) at a dilution of 1:500 overnight at º°C, washed in TBST, and probed with a HRP-conjugated goat anti-rabbit IgG at a dilution of 1:40,000 for 1 hr at room temperature. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.
quantity: 100 uL
price: US 454.00
to the supplier

Phospho-SIRT1 (Ser47) Polyclonal Antibody
Invitrogen
catalog: PA5-17391

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 1

quantity: 100 uL
price: US 464.00
to the supplier

SIRT1 Polyclonal Antibody
Invitrogen
catalog: PA5-23063

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

quantity: 100 uL
price: US 474.00
to the supplier

SIRT1 Monoclonal Antibody (OTI5B2)
Invitrogen
catalog: MA5-27217

mouse monoclonal (OTI5B2)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

quantity: 100 uL
price: US 542.00
to the supplier

SIRT1 Recombinant Rabbit Monoclonal Antibody (012)
Invitrogen
catalog: MA5-30879

domestic rabbit recombinant (12)
reactivity: human
application: ELISA
citations: 1

quantity: 100 uL
price: US 414.00
to the supplier

SIRT1 Polyclonal Antibody
Invitrogen
catalog: PA5-17393

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

quantity: 100 uL
price: US 404.00
to the supplier

SIRT1 Monoclonal Antibody (OTI1H1)
Invitrogen
catalog: MA5-27216

mouse monoclonal (OTI1H1)
reactivity: human, mouse
application: western blot

quantity: 100 uL
price: US 552.00
to the supplier

SIRT1 Recombinant Rabbit Monoclonal Antibody (2H9L18)
Invitrogen
catalog: 703653

domestic rabbit recombinant (2H9L18)
reactivity: human
application: immunocytochemistry

quantity: 100 ug
price: US 520.00
to the supplier

SIRT1 Recombinant Rabbit Monoclonal Antibody (4H9L12)
Invitrogen
catalog: 703368

domestic rabbit recombinant (4H9L12)
reactivity: human
application: western blot

quantity: 100 ug
price: US 520.00
to the supplier

SIRT1 Monoclonal Antibody (3H10A10)
Invitrogen
catalog: MA5-29570

mouse monoclonal (3H10A10)
reactivity: human
application: ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 323.00
to the supplier

SIRT1 Recombinant Rabbit Monoclonal Antibody (032)
Invitrogen
catalog: MA5-30880

domestic rabbit recombinant (32)
reactivity: human
application: ELISA

quantity: 100 uL
price: US 414.00
to the supplier

SIRT1 Recombinant Rabbit Monoclonal Antibody (SZ04-01)
Invitrogen
catalog: MA5-32030

domestic rabbit recombinant (SZ04-01)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 474.00
to the supplier

Phospho-SIRT1 (Ser47) Recombinant Rabbit Monoclonal Antibody (SN06-40)
Invitrogen
catalog: MA5-32283

domestic rabbit recombinant (SN06-40)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 474.00
to the supplier

Phospho-SIRT1 (Thr530) Recombinant Rabbit Monoclonal Antibody (JJ206-6)
Invitrogen
catalog: MA5-32435

domestic rabbit recombinant (JJ206-6)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 474.00
to the supplier

SIRT1 Monoclonal Antibody (7-C5-B2)
Invitrogen
catalog: MA5-32912

mouse monoclonal (7-C5-B2)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 474.00
to the supplier

SIRT1 Polyclonal Antibody
Invitrogen
catalog: PA5-85921

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

SIRT1 Polyclonal Antibody
Invitrogen
catalog: PA5-95201

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

quantity: 100 ug
price: US 464.00
to the supplier

SIRT1 Polyclonal Antibody
Invitrogen
catalog: PA5-87383

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry

quantity: 100 uL
price: US 464.00
to the supplier

Phospho-SIRT1 (Ser47) Recombinant Rabbit Monoclonal Antibody (ARC1520)
Invitrogen
catalog: MA5-35837

domestic rabbit recombinant (ARC1520)
reactivity: human
application: western blot, ELISA

quantity: 100 uL
price: US 491.00
to the supplier

Phospho-SIRT1 (Ser47) Polyclonal Antibody
Invitrogen
catalog: PA5-105025

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunocytochemistry

quantity: 100 uL
price: US 464.00
to the supplier

Phospho-SIRT1 (Ser27) Polyclonal Antibody
Invitrogen
catalog: PA5-105222

domestic rabbit polyclonal
reactivity: human
application: western blot

quantity: 100 uL
price: US 464.00
to the supplier

SIRT1 Recombinant Rabbit Monoclonal Antibody (JE11-04)
Invitrogen
catalog: MA5-45000

domestic rabbit recombinant (JE11-04)
reactivity: human
application: western blot, immunocytochemistry

quantity: 100 uL
price: US 470.00
to the supplier

SIRT1 Polyclonal Antibody
Invitrogen
catalog: PA5-116530

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 474.00
to the supplier

SIRT1 Polyclonal Antibody
Invitrogen
catalog: PA5-119604

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 460.00
to the supplier

SIRT1 Polyclonal Antibody
Invitrogen
catalog: PA5-120542

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry

quantity: 100 uL
price: US 460.00
to the supplier

SIRT1 Polyclonal Antibody
Invitrogen
catalog: PA5-142717

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 ug
price: US 444.00
to the supplier