TIMP2 Monoclonal Antibody (3A4), Biotin
Invitrogen
catalog: MA5-12204

mouse monoclonal (3A4)
reactivity: guinea pig, human, mouse, rat, bovine, domestic rabbit
conjugate: biotin
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 37

Formalin-fixed, paraffin-embedded human pancreatic tumor stained with TIMP-2 antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.

quantity: 500 uL
price: US 484.00
to the supplier

TIMP2 Monoclonal Antibody (F27 P3 A4)
Invitrogen
catalog: MA1-774

mouse monoclonal (F27 P3 A4)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 5

Immunohistochemistry was performed on normal biopsies of deparaffinized Human pancreas tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a TIMP2 monoclonal antibody (Product # MA1-774) at a dilution of 1:500 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse pancreas tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a TIMP2 monoclonal antibody (Product # MA1-774) at a dilution of 1:100 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Immunofluorescent analysis of TIMP2 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a TIMP2 monoclonal antibody (Product # MA1-774) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). TIMP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
quantity: 100 ug
price: US 491.00
to the supplier

TIMP2 Monoclonal Antibody (T2-101)
Invitrogen
catalog: MA5-13603

mouse monoclonal (T2-101)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunoprecipitation, other
citations: 5

quantity: 500 uL
price: US 524.00
to the supplier

TIMP2 Polyclonal Antibody
Invitrogen
catalog: PA5-69159

domestic rabbit polyclonal
reactivity: mouse
application: western blot

quantity: 100 uL
price: US 464.00
to the supplier

TIMP2 Polyclonal Antibody
Invitrogen
catalog: PA5-86216

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

TIMP2 Polyclonal Antibody
Invitrogen
catalog: PA5-94943

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 ug
price: US 464.00
to the supplier

TIMP2 Polyclonal Antibody
Invitrogen
catalog: PA5-95330

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

quantity: 100 ug
price: US 454.00
to the supplier

TIMP2 Polyclonal Antibody
Invitrogen
catalog: PA5-105300

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

TIMP2 Recombinant Rabbit Monoclonal Antibody (5P3T3)
Invitrogen
catalog: MA5-51199

domestic rabbit recombinant (5P3T3)
reactivity: human, mouse, rat
application: western blot, ELISA

quantity: 100 uL
price: US 424.00
to the supplier

TIMP2 Recombinant Rabbit Monoclonal Antibody (4N9N3)
Invitrogen
catalog: MA5-55344

domestic rabbit recombinant (4N9N3)
reactivity: human, mouse, rat
application: western blot, ELISA

quantity: 100 uL
price: US 419.00
to the supplier