STAT3 Monoclonal Antibody (9D8)
Invitrogen
catalog: MA1-13042
mouse monoclonal (9D8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 22

Immunofluorescent analysis of STAT3 using anti-STAT3 (9D8) monoclonal antibody (Product# MA1-13042) (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Product #37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing STAT3 (Product# MA1-13042), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product# 35503) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

Immunohistochemistry was performed on biopsies of normal and cancer tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95°C. Following antigen retrieval tissues were blocked in 10% normal goat serum (Product #31873) for 20 minutes at room temperature. Tissues were then probed at a dilution of 1:1600 with a mouse monoclonal antibody recognizing STAT3 (Product # MA1-13042) overnight at 4°C in a humidified chamber. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 400X magnification. Results demonstrate both nuclear and cytoplasmic localization of STAT3.

Western blot analysis of STAT3 was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing STAT3 (Product # MA1-13042) at a dilution of 1:5000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 32430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Dura (Product #34075).
quantity: 100 uL
price: US 520.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 9
quantity: 100 uL
price: US 520.00
to the supplier
mouse monoclonal (LUVNKLA)
reactivity: human, mouse
conjugate: APC
application: flow cytometry
citations: 13
quantity: 25 Tests
price: US 174.00
to the supplier
mouse monoclonal (LUVNKLA)
reactivity: human, mouse
conjugate: APC
application: flow cytometry
citations: 13
quantity: 100 Tests
price: US 431.00
to the supplier
mouse monoclonal (LUVNKLA)
reactivity: human, mouse
conjugate: PE
application: flow cytometry
citations: 12
quantity: 25 Tests
price: US 174.00
to the supplier
mouse monoclonal (LUVNKLA)
reactivity: human, mouse
conjugate: PE
application: flow cytometry
citations: 12
quantity: 100 Tests
price: US 427.00
to the supplier
domestic rabbit monoclonal (3HCLC)
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 4
quantity: 100 ug
price: US 520.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot
citations: 4
quantity: 100 uL
price: US 520.00
to the supplier
mouse monoclonal (LUVNKLA)
reactivity: human, mouse
conjugate: FITC
application: flow cytometry
citations: 6
quantity: 25 Tests
price: US 177.00
to the supplier
mouse monoclonal (LUVNKLA)
reactivity: human, mouse
conjugate: PE/Cy7
application: flow cytometry
citations: 6
quantity: 25 Tests
price: US 188.00
to the supplier
mouse monoclonal (LUVNKLA)
reactivity: human, mouse
conjugate: FITC
application: flow cytometry
citations: 6
quantity: 100 Tests
price: US 421.00
to the supplier
mouse monoclonal (LUVNKLA)
reactivity: human, mouse
conjugate: eFluor 450
application: flow cytometry
citations: 6
quantity: 100 Tests
price: US 434.00
to the supplier
mouse monoclonal (LUVNKLA)
reactivity: human, mouse
conjugate: PE/Cy7
application: flow cytometry
citations: 6
quantity: 100 Tests
price: US 446.00
to the supplier
STAT3 Polyclonal Antibody
Invitrogen
catalog: PA1-86605
domestic goat polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 2
quantity: 100 ug
price: US 522.00
to the supplier
mouse monoclonal (R.263.6)
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunoprecipitation, flow cytometry
citations: 2


quantity: 100 uL
price: US 524.00
to the supplier
Phospho-STAT3 (Ser727) Polyclonal Antibody
Invitrogen
catalog: PA5-17876
domestic rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunocytochemistry, immunoprecipitation
citations: 3


quantity: 100 uL
price: US 514.00
to the supplier
mouse monoclonal (LUVNKLA)
reactivity: human, mouse
conjugate: Percp/efluor 710
application: flow cytometry
citations: 2
quantity: 100 Tests
price: US 434.00
to the supplier
mouse monoclonal (E.35.9)
reactivity: human, mouse
modification: phosphorylated
application: western blot, immunocytochemistry
citations: 1

quantity: 100 uL
price: US 524.00
to the supplier
STAT3 Monoclonal Antibody (232209), PE
Invitrogen
catalog: MA5-23569
mouse monoclonal (232209)
reactivity: human, mouse, rat
conjugate: PE
application: flow cytometry
citations: 1
quantity: 100 Tests
price: US 350.00
to the supplier
STAT3 Monoclonal Antibody (232209), FITC
Invitrogen
catalog: MA5-23635
mouse monoclonal (232209)
reactivity: human, mouse, rat
conjugate: FITC
application: flow cytometry
citations: 1
quantity: 100 Tests
price: US 442.00
to the supplier
STAT3 Polyclonal Antibody
Invitrogen
catalog: PA5-18562
domestic goat polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
domestic rabbit recombinant (SY24-09)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 484.00
to the supplier
STAT3 Polyclonal Antibody
Invitrogen
catalog: PA5-85199
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
quantity: 100 uL
price: US 464.00
to the supplier
Phospho-STAT3 (Tyr705) Polyclonal Antibody
Invitrogen
catalog: PA5-85445
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 460.00
to the supplier
Phospho-STAT3 (Tyr705) Polyclonal Antibody
Invitrogen
catalog: PA5-85801
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
quantity: 100 uL
price: US 464.00
to the supplier
STAT3 Polyclonal Antibody
Invitrogen
catalog: PA5-86074
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation
quantity: 100 uL
price: US 464.00
to the supplier
STAT3 Polyclonal Antibody
Invitrogen
catalog: PA5-86075
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation
quantity: 100 uL
price: US 464.00
to the supplier
STAT3 Polyclonal Antibody
Invitrogen
catalog: PA5-86208
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 454.00
to the supplier
domestic rabbit recombinant (ARC0150)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 480.00
to the supplier
domestic rabbit recombinant (6W3L3)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
Phospho-STAT3 (Tyr539) Polyclonal Antibody
Invitrogen
catalog: PA5-105568
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
domestic rabbit recombinant (Stat3Y705-B12)
reactivity: human, mouse
application: flow cytometry
quantity: 200 uL
price: US 626.00
to the supplier
domestic rabbit recombinant (Stat3Y705-B12)
reactivity: human, mouse
conjugate: PE
application: flow cytometry
quantity: 100 Tests
price: US 474.00
to the supplier
domestic rabbit recombinant (Stat3Y705-B12)
reactivity: human, mouse
conjugate: FITC
application: flow cytometry
quantity: 100 Tests
price: US 484.00
to the supplier
domestic rabbit recombinant (Stat3Y705-B12)
reactivity: human, mouse
conjugate: APC
application: flow cytometry
quantity: 100 Tests
price: US 484.00
to the supplier
domestic rabbit recombinant (Stat3Y705-B12)
reactivity: human, mouse
application: flow cytometry
quantity: 20 uL
price: US 329.00
to the supplier
domestic rabbit recombinant (Stat3Y705-B12)
reactivity: human, mouse
conjugate: PE
application: flow cytometry
quantity: 10 Tests
price: US 191.00
to the supplier
domestic rabbit recombinant (Stat3Y705-B12)
reactivity: human, mouse
conjugate: FITC
application: flow cytometry
quantity: 10 Tests
price: US 197.00
to the supplier
domestic rabbit recombinant (Stat3Y705-B12)
reactivity: human, mouse
conjugate: APC
application: flow cytometry
quantity: 10 Tests
price: US 191.00
to the supplier
domestic rabbit recombinant (SY34-01)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
domestic rabbit recombinant (SY34-01)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
domestic rabbit recombinant (SZ43-01)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
domestic rabbit recombinant (6T6X9)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
STAT3 Polyclonal Antibody
Invitrogen
catalog: PA5-120138
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 460.00
to the supplier
Phospho-STAT3 (Tyr705) Polyclonal Antibody
Invitrogen
catalog: PA5-121259
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 460.00
to the supplier
Phospho-STAT3 (Ser727) Polyclonal Antibody
Invitrogen
catalog: PA5-121277
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 460.00
to the supplier
domestic rabbit recombinant (23GB4205)
reactivity: human, mouse, rat
application: western blot
quantity: 100 uL
price: US 539.00
to the supplier
domestic rabbit recombinant (23GB4205)
reactivity: human, mouse, rat
application: western blot
quantity: 20 uL
price: US 199.00
to the supplier
domestic rabbit recombinant (23GB990)
reactivity: human, mouse, rat
application: western blot, flow cytometry
quantity: 100 uL
price: US 539.00
to the supplier
domestic rabbit recombinant (23GB990)
reactivity: human, mouse, rat
application: western blot, flow cytometry
quantity: 20 uL
price: US 199.00
to the supplier
mouse recombinant (LUVNKLA)
reactivity: human, mouse
application: western blot, flow cytometry
quantity: 100 uL
price: US 349.00
to the supplier
mouse recombinant (LUVNKLA)
reactivity: human, mouse
application: flow cytometry
quantity: 50 uL
price: US 329.00
to the supplier
mouse recombinant (LUVNKLA)
reactivity: human, mouse
application: flow cytometry
quantity: 20 uL
price: US 199.00
to the supplier
mouse recombinant (LUVNKLA)
reactivity: human, mouse
application: flow cytometry
quantity: 50 uL
price: US 329.00
to the supplier
mouse recombinant (LUVNKLA)
reactivity: human, mouse
application: flow cytometry
quantity: 20 uL
price: US 189.00
to the supplier
mouse recombinant (LUVNKLA)
reactivity: human, mouse
application: flow cytometry
quantity: 50 uL
price: US 329.00
to the supplier
mouse recombinant (LUVNKLA)
reactivity: human, mouse
application: flow cytometry
quantity: 20 uL
price: US 189.00
to the supplier
mouse recombinant (LUVNKLA)
reactivity: human, mouse
application: western blot, flow cytometry
quantity: 20 uL
price: US 189.00
to the supplier
domestic rabbit recombinant (HL1134)
reactivity: human, mouse, rat, dogs, cat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 479.00
to the supplier