AKT Pan Polyclonal Antibody
Invitrogen
catalog: 44-609G
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 31
quantity: 100 uL
price: US 520.00
to the supplier
AKT Pan Monoclonal Antibody (J.314.4)
Invitrogen
catalog: MA5-14916
domestic rabbit monoclonal (J.314.4)
reactivity: human, mouse, rat, fruit fly
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 7

quantity: 100 uL
price: US 524.00
to the supplier
AKT2 Monoclonal Antibody (ZA006)
Invitrogen
catalog: 39-3900
mouse monoclonal (ZA006)
reactivity: human, rat
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
citations: 2
quantity: 100 ug
price: US 414.00
to the supplier
Phospho-AKT1/AKT2/AKT3 (Ser473) Polyclonal Antibody
Invitrogen
catalog: PA5-104445
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 2
quantity: 100 uL
price: US 440.00
to the supplier
AKT Pan Monoclonal Antibody (E.32.10)
Invitrogen
catalog: MA5-14999
domestic rabbit monoclonal (E.32.10)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 1


quantity: 100 uL
price: US 484.00
to the supplier
Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Polyclonal Antibody
Invitrogen
catalog: PA5-36780
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1
quantity: 100 uL
price: US 464.00
to the supplier
Phospho-AKT2 (Ser474) Polyclonal Antibody
Invitrogen
catalog: PA5-104870
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1
quantity: 100 uL
price: US 454.00
to the supplier
Phospho-AKT1/AKT2/AKT3 (Ser473) Recombinant Rabbit Monoclonal Antibody (2E3A3)
Invitrogen
catalog: MA5-38243
domestic rabbit recombinant (2E3A3)
reactivity: human, mouse, rat
application: western blot, ELISA
citations: 1
quantity: 100 uL
price: US 484.00
to the supplier
AKT2 Monoclonal Antibody (95C657.1.2)
Invitrogen
catalog: MA1-41115
mouse monoclonal (95C657.1.2)
reactivity: human
application: western blot, flow cytometry

quantity: 200 uL
price: US 474.00
to the supplier
AKT2 Monoclonal Antibody (S.595.2)
Invitrogen
catalog: MA5-14807
domestic rabbit monoclonal (S.595.2)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
quantity: 100 uL
price: US 414.00
to the supplier
AKT2 Monoclonal Antibody (1B6)
Invitrogen
catalog: MA5-15593
mouse monoclonal (1B6)
reactivity: human, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunohistochemical analysis of paraffin-embedded human Thymus tissues using AKT2 antibody

Immunofluorescence analysis of PANC-1 (left) and Hela (right) cells using AKT2 antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
quantity: 100 uL
price: US 491.00
to the supplier
AKT2 Polyclonal Antibody
Invitrogen
catalog: PA5-13759
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section


quantity: 400 uL
price: US 464.00
to the supplier
AKT2 Polyclonal Antibody
Invitrogen
catalog: PA5-13760
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section


quantity: 400 uL
price: US 474.00
to the supplier
AKT2 Polyclonal Antibody
Invitrogen
catalog: PA5-13761
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 400 uL
price: US 474.00
to the supplier
AKT2 Monoclonal Antibody (4H7)
Invitrogen
catalog: MA1-034X
mouse monoclonal (4H7)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Immunohistochemistry was performed on biopsies of deparaffinized normal human medulla oblongata and human esophageal cancer tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95C. Following antigen retrieval tissues were blocked in 10% normal goat serum (Product #31873) for 20 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Akt-2 (Product # MA1-034) overnight at 4C in a humidified chamber. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 400X magnification. Results demonstrate cytoplasmic localization of Akt-2.

Immunoprecipitation of Akt-2 was performed on HeLa cells. The antigen:antibody complex was formed by incubating 750µg whole cell lysate with 2µg of mouse monoclonal antibody recognizing Akt-2 (Product # MA1-034) overnight on a rocking platform at 4°C. The immune-complex was then captured on 50µl Protein A/G Plus Agarose (Product #20423) . Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye (Product # 39000). Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing Akt-2 (Product # MA1-034) at a dilution of 1:1000 overnight rotating at 4C. Membranes were washed in TBST and probed with Clean Blot IP Detection reagent (Product # 21230) at a dilution of 1:2000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product #34075).

Western blot analysis of Akt-2 was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing Akt-2 (Product # MA1-034) at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product #34075).
quantity: 20 uL
price: US 177.00
to the supplier
AKT Pan Polyclonal Antibody
Invitrogen
catalog: PA5-31915
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry

Immunohistochemical staining of Human esophageal cancer tissue sections using anti-AKT1+2+3 antibody (PA5-31915).

Western Blot analysis of AKT1+2+3 using anti-AKT1+2+3 Polyclonal Antibody (PA5-31915), in A431 Cell Lysate (30 ug of cell lysate) and at a dilution of 1:5000.
quantity: 100 uL
price: US 464.00
to the supplier
AKT2 Monoclonal Antibody (4H7)
Invitrogen
catalog: MA1-034
mouse monoclonal (4H7)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
quantity: 100 ug
price: US 491.00
to the supplier
Phospho-AKT Pan (Thr450, Thr451, Thr447) Polyclonal Antibody
Invitrogen
catalog: PA5-36728
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 454.00
to the supplier
Phospho-AKT2 (Ser474) Polyclonal Antibody
Invitrogen
catalog: PA5-36819
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 481.00
to the supplier
Phospho-AKT2 (Ser474) Polyclonal Antibody
Invitrogen
catalog: PA5-37470
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Polyclonal Antibody
Invitrogen
catalog: PA5-37814
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 454.00
to the supplier
AKT Pan Recombinant Superclonal Antibody (8HCLC)
Invitrogen
catalog: 711248
domestic rabbit monoclonal (8HCLC)
reactivity: human
application: western blot, immunocytochemistry
quantity: 100 ug
price: US 414.00
to the supplier
AKT Pan Polyclonal Antibody
Invitrogen
catalog: PA5-77855
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
quantity: 100 uL
price: US 470.00
to the supplier
AKT2 Polyclonal Antibody
Invitrogen
catalog: PA5-85321
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
AKT2 Polyclonal Antibody
Invitrogen
catalog: PA5-85517
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100 uL
price: US 464.00
to the supplier
AKT2 Polyclonal Antibody
Invitrogen
catalog: PA5-85518
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
AKT2 Polyclonal Antibody
Invitrogen
catalog: PA5-95178
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
quantity: 100 ug
price: US 464.00
to the supplier
AKT2 Polyclonal Antibody
Invitrogen
catalog: PA5-88591
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
AKT Pan Polyclonal Antibody
Invitrogen
catalog: PA5-104548
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
Phospho-AKT1/AKT2/AKT3 (Thr308) Polyclonal Antibody
Invitrogen
catalog: PA5-104869
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 414.00
to the supplier
Phospho-AKT1/AKT2/AKT3 (Ser473) Polyclonal Antibody
Invitrogen
catalog: PA5-110107
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
Phospho-AKT1/AKT2/AKT3 (Ser473) Polyclonal Antibody
Invitrogen
catalog: PA5-99331
domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 414.00
to the supplier
AKT Pan Recombinant Rabbit Monoclonal Antibody (ST48-09)
Invitrogen
catalog: MA5-41139
domestic rabbit recombinant (ST48-09)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
AKT1/AKT2 Recombinant Rabbit Monoclonal Antibody (1G0K5)
Invitrogen
catalog: MA5-42793
domestic rabbit recombinant (1G0K5)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
AKT Pan Recombinant Rabbit Monoclonal Antibody (16E10)
Invitrogen
catalog: MA5-50173
domestic rabbit recombinant (1.60E+11)
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 414.00
to the supplier
AKT Pan Recombinant Rabbit Monoclonal Antibody (3K3H0)
Invitrogen
catalog: MA5-51195
domestic rabbit recombinant (3K3H0)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 424.00
to the supplier
Phospho-AKT Pan (Thr450, Thr451, Thr447) Recombinant Rabbit Monoclonal Antibody ...
Invitrogen
catalog: MA5-51537
domestic rabbit recombinant (8U6D2)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 424.00
to the supplier
Phospho-AKT2 (Ser474) Polyclonal Antibody
Invitrogen
catalog: PA5-119813
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 460.00
to the supplier
Phospho-AKT2 (Ser474) Polyclonal Antibody
Invitrogen
catalog: PA5-121255
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
quantity: 100 uL
price: US 460.00
to the supplier
Phospho-AKT Pan (Thr308, Thr305, Thr309) Polyclonal Antibody
Invitrogen
catalog: PA5-121345
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA
quantity: 100 uL
price: US 460.00
to the supplier
AKT2 Polyclonal Antibody
Invitrogen
catalog: PA5-142151
domestic goat polyclonal
reactivity: human
application: western blot
quantity: 100 ug
price: US 444.00
to the supplier
AKT Pan Recombinant Rabbit Monoclonal Antibody (23GB3180)
Invitrogen
catalog: MA5-52546
domestic rabbit recombinant (23GB3180)
reactivity: human, mouse, rat
application: western blot, flow cytometry
quantity: 100 uL
price: US 429.00
to the supplier
AKT Pan Recombinant Rabbit Monoclonal Antibody (23GB3180)
Invitrogen
catalog: MA5-52547
domestic rabbit recombinant (23GB3180)
reactivity: human, mouse, rat
application: western blot, flow cytometry
quantity: 20 uL
price: US 179.00
to the supplier
AKT Pan Recombinant Rabbit Monoclonal Antibody (23GB3185)
Invitrogen
catalog: MA5-52548
domestic rabbit recombinant (23GB3185)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry
quantity: 100 uL
price: US 429.00
to the supplier
AKT Pan Recombinant Rabbit Monoclonal Antibody (23GB3185)
Invitrogen
catalog: MA5-52549
domestic rabbit recombinant (23GB3185)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry
quantity: 20 uL
price: US 179.00
to the supplier
Phospho-AKT1/AKT2/AKT3 (Ser473) Recombinant Rabbit Monoclonal Antibody (PSH04-44 ...
Invitrogen
catalog: MA5-56569
domestic rabbit recombinant (PSH04-44)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 479.00
to the supplier