Phospho-eIF4E (Ser209) Polyclonal Antibody
Invitrogen
catalog: 44-528G

domestic rabbit polyclonal
reactivity: human, mouse, rat, domestic rabbit
modification: phosphorylated
application: western blot, immunocytochemistry, immunoprecipitation, RNA immunoprecipitation
citations: 4

quantity: 100 uL
price: US 520.00
to the supplier

eIF4E Monoclonal Antibody (5D11)
Invitrogen
catalog: MA1-089

mouse monoclonal (5D11)
reactivity: human, mouse
application: western blot, immunocytochemistry, immunoprecipitation, RNA immunoprecipitation
citations: 2

Immunoprecipitation of eIF4E was performed on HeLa cell lysates. The antigen:antibody complex was formed by incubating 500µg whole cell lysate with 3µg of mouse monoclonal antibody recognizing eIF4E (Product # MA1-089) overnight on a rocking platform at 4°C. The immune-complex was captured on 50µl Protein A/G Plus Agarose (Product #20423). Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing eIF4E (Product # MA1-089) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBST and probed with Pierce Clean Blot (Product #21230) at a dilution of 1:1,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product #34075).

Western blot analysis of eIF4E was performed by loading 80µg whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing eIF4E (Product #MA1-089) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:20000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Pico (Product #34080).
quantity: 100 ug
price: US 502.00
to the supplier

Phospho-eIF4E (Ser209) Polyclonal Antibody
Invitrogen
catalog: PA5-17919

domestic rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunocytochemistry

quantity: 100 uL
price: US 464.00
to the supplier

eIF4E Polyclonal Antibody
Invitrogen
catalog: PA5-11797

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 400 uL
price: US 464.00
to the supplier

eIF4E Polyclonal Antibody
Invitrogen
catalog: PA5-24582

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of EIF4E in HL-60 cell line lysates using 35ug per lane of antibody.

Immunohistochemical analysis in formalin-fixed and paraffin-embedded human testis tissue followed by peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
quantity: 400 uL
price: US 464.00
to the supplier

eIF4E Polyclonal Antibody
Invitrogen
catalog: PA5-41751

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

quantity: 100 uL
price: US 464.00
to the supplier

eIF4E Polyclonal Antibody
Invitrogen
catalog: PA5-77960

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 474.00
to the supplier

eIF4E Polyclonal Antibody
Invitrogen
catalog: PA5-84061

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

quantity: 100 uL
price: US 474.00
to the supplier

Phospho-eIF4E (Ser209) Polyclonal Antibody
Invitrogen
catalog: PA5-85816

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

quantity: 100 uL
price: US 464.00
to the supplier

eIF4E Polyclonal Antibody
Invitrogen
catalog: PA5-86679

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

eIF4E Polyclonal Antibody
Invitrogen
catalog: PA5-86554

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

eIF4E Polyclonal Antibody
Invitrogen
catalog: PA5-87442

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry

quantity: 100 uL
price: US 464.00
to the supplier

Phospho-eIF4E (Ser209) Recombinant Rabbit Monoclonal Antibody (1F3)
Invitrogen
catalog: MA5-33134

domestic rabbit recombinant (1F3)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 491.00
to the supplier

Phospho-eIF4E (Thr210) Polyclonal Antibody
Invitrogen
catalog: PA5-105472

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

eIF4E Polyclonal Antibody
Invitrogen
catalog: PA5-109681

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

eIF4E Monoclonal Antibody (163CT48.1.9)
Invitrogen
catalog: MA5-37479

mouse monoclonal (163CT48.1.9)
reactivity: human, mouse
application: western blot, immunocytochemistry

quantity: 400 uL
price: US 474.00
to the supplier

eIF4E Monoclonal Antibody (1504CT774.14.55)
Invitrogen
catalog: MA5-37678

mouse monoclonal (1504CT774.14.55)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry

quantity: 400 uL
price: US 474.00
to the supplier

Phospho-eIF4E (Ser209) Recombinant Rabbit Monoclonal Antibody (SU0396)
Invitrogen
catalog: MA5-41151

domestic rabbit recombinant (SU0396)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 470.00
to the supplier

eIF4E Recombinant Rabbit Monoclonal Antibody (HL1553)
Invitrogen
catalog: MA5-47094

domestic rabbit recombinant (HL1553)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 444.00
to the supplier

eIF4E Recombinant Rabbit Monoclonal Antibody (HL1554)
Invitrogen
catalog: MA5-50319

domestic rabbit recombinant (HL1554)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 460.00
to the supplier

eIF4E Recombinant Rabbit Monoclonal Antibody (HL1555)
Invitrogen
catalog: MA5-50320

domestic rabbit recombinant (HL1555)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 460.00
to the supplier

eIF4E Recombinant Rabbit Monoclonal Antibody (JE60-70)
Invitrogen
catalog: MA5-50717

domestic rabbit recombinant (JE60-70)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 470.00
to the supplier

Phospho-eIF4E (Ser209) Recombinant Rabbit Monoclonal Antibody (0I0D10)
Invitrogen
catalog: MA5-55315

domestic rabbit recombinant (0I0D10)
reactivity: human, rat
application: western blot, ELISA

quantity: 100 uL
price: US 419.00
to the supplier