CD45 Monoclonal Antibody (2D1), APC, eBioscience | Invitrogen 17-9459-41 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD45 Monoclonal Antibody (2D1), APC, eBioscience

catalog :

17-9459-41

quantity :

25 Tests

price :

US 127

clonality :

monoclonal

host :

mouse

conjugate :

APC

clone name :

2D1

reactivity :

human, mouse

application :

flow cytometry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:500; loading ...; fig 2e	Shao Q, Esseltine J, Huang T, Novielli Kuntz N, Ching J, SAMPSON J, et al. Connexin43 is Dispensable for Early Stage Human Mesenchymal Stem Cell Adipogenic Differentiation But is Protective against Cell Senescence. Biomolecules. 2019;9: pubmed publisher
flow cytometry; mouse; loading ...; fig 6c	Su S, Liao J, Liu J, Huang D, He C, Chen F, et al. Blocking the recruitment of naive CD4+ T cells reverses immunosuppression in breast cancer. Cell Res. 2017;27:461-482 pubmed publisher
flow cytometry; human; fig 5	Severson J, Serracino H, Mateescu V, Raeburn C, McIntyre R, Sams S, et al. PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer. Cancer Immunol Res. 2015;3:620-30 pubmed publisher
immunohistochemistry - frozen section; mouse	Wilson E, Bial J, Tarlow B, Bial G, Jensen B, Greiner D, et al. Extensive double humanization of both liver and hematopoiesis in FRGN mice. Stem Cell Res. 2014;13:404-12 pubmed publisher
	Jiang W, Zhang J, Zhang X, Fan C, Huang J. VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway. Bioengineered. 2021;12:10264-10284 pubmed publisher
	Xiao K, Yang L, Xie W, Gao X, Huang R, Xie M. Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF-β/BMP expression levels. Exp Ther Med. 2021;22:736 pubmed publisher
	Bazié W, Boucher J, Vitry J, Goyer B, Routy J, Tremblay C, et al. Plasma Extracellular Vesicle Subtypes May be Useful as Potential Biomarkers of Immune Activation in People With HIV. Pathog Immun. 2021;6:1-28 pubmed publisher
	Verbeke D, Demeyer S, Prieto C, de Bock C, De Bie J, Gielen O, et al. The XPO1 Inhibitor KPT-8602 Synergizes with Dexamethasone in Acute Lymphoblastic Leukemia. Clin Cancer Res. 2020;26:5747-5758 pubmed publisher
	Sun L, Zhu W, Zhao P, Zhang J, Lu Y, Zhu Y, et al. Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair. Front Cell Dev Biol. 2020;8:263 pubmed publisher
	Guedan S, Madar A, Casado Medrano V, Shaw C, Wing A, Liu F, et al. Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability. J Clin Invest. 2020;130:3087-3097 pubmed publisher
	Hashwah H, Bertram K, Stirm K, Stelling A, Wu C, Kasser S, et al. The IL-6 signaling complex is a critical driver, negative prognostic factor, and therapeutic target in diffuse large B-cell lymphoma. EMBO Mol Med. 2019;11:e10576 pubmed publisher
	Fan C, Ji Q, Zhang C, Xu S, Sun H, Li Z. TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production. Mol Med Rep. 2019;20:3123-3130 pubmed publisher
	Ormel P, Vieira de Sá R, van Bodegraven E, Karst H, Harschnitz O, Sneeboer M, et al. Microglia innately develop within cerebral organoids. Nat Commun. 2018;9:4167 pubmed publisher
	Li X, Ma R, Gu Q, Liang L, Wang L, Zhang Y, et al. A fully defined static suspension culture system for large-scale human embryonic stem cell production. Cell Death Dis. 2018;9:892 pubmed publisher
	Narasimhan P, Akabas L, Tariq S, Huda N, Bennuru S, Sabzevari H, et al. Similarities and differences between helminth parasites and cancer cell lines in shaping human monocytes: Insights into parallel mechanisms of immune evasion. PLoS Negl Trop Dis. 2018;12:e0006404 pubmed publisher
	Reyna D, Garner T, Lopez A, Kopp F, Choudhary G, Sridharan A, et al. Direct Activation of BAX by BTSA1 Overcomes Apoptosis Resistance in Acute Myeloid Leukemia. Cancer Cell. 2017;32:490-505.e10 pubmed publisher
	Gaskill C, Majka S. A high-yield isolation and enrichment strategy for human lung microvascular endothelial cells. Pulm Circ. 2017;7:108-116 pubmed publisher
	Subramanian S, Pallati P, Rai V, Sharma P, Agrawal D, Nandipati K. Increased expression of triggering receptor expressed on myeloid cells-1 in the population with obesity and insulin resistance. Obesity (Silver Spring). 2017;25:527-538 pubmed publisher
	Senbabaoglu Y, Gejman R, Winer A, Liu M, Van Allen E, de Velasco G, et al. Tumor immune microenvironment characterization in clear cell renal cell carcinoma identifies prognostic and immunotherapeutically relevant messenger RNA signatures. Genome Biol. 2016;17:231 pubmed
	Cermak N, Olcum S, Delgado F, Wasserman S, Payer K, A Murakami M, et al. High-throughput measurement of single-cell growth rates using serial microfluidic mass sensor arrays. Nat Biotechnol. 2016;34:1052-1059 pubmed publisher
	Oelsner S, Wagner J, Friede M, Pfirrmann V, Genßler S, Rettinger E, et al. Chimeric antigen receptor-engineered cytokine-induced killer cells overcome treatment resistance of pre-B-cell acute lymphoblastic leukemia and enhance survival. Int J Cancer. 2016;139:1799-809 pubmed publisher
	Weisberg E, Halilovic E, Cooke V, Nonami A, Ren T, Sanda T, et al. Inhibition of Wild-Type p53-Expressing AML by the Novel Small Molecule HDM2 Inhibitor CGM097. Mol Cancer Ther. 2015;14:2249-59 pubmed publisher
	Brandhorst S, Choi I, Wei M, Cheng C, Sedrakyan S, Navarrete G, et al. A Periodic Diet that Mimics Fasting Promotes Multi-System Regeneration, Enhanced Cognitive Performance, and Healthspan. Cell Metab. 2015;22:86-99 pubmed publisher
	Gori J, Butler J, Chan Y, Chandrasekaran D, Poulos M, Ginsberg M, et al. Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells. J Clin Invest. 2015;125:1243-54 pubmed publisher
	Gallipoli P, Cook A, Rhodes S, Hopcroft L, Wheadon H, Whetton A, et al. JAK2/STAT5 inhibition by nilotinib with ruxolitinib contributes to the elimination of CML CD34+ cells in vitro and in vivo. Blood. 2014;124:1492-501 pubmed publisher
	Mendez J, Ghaedi M, Steinbacher D, Niklason L. Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds. Tissue Eng Part A. 2014;20:1735-46 pubmed publisher
	Liu S, Li N, Yu X, Xiao X, Cheng K, Hu J, et al. Expression of intercellular adhesion molecule 1 by hepatocellular carcinoma stem cells and circulating tumor cells. Gastroenterology. 2013;144:1031-1041.e10 pubmed publisher

product information

Product Type :

Antibody

Product Name :

CD45 Monoclonal Antibody (2D1), APC, eBioscience

Catalog # :

17-9459-41

Quantity :

25 Tests

Pricing :

US 127

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 5 µL (0.25 µg)/test

Species :

Human

Clone :

2D1

Isotype :

IgG1, kappa

Storage :

4° C, store in dark, DO NOT FREEZE!

Description :

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 5 µL (0.25 µg)/test

Aliases :

B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

more info or order :

Invitrogen product webpage