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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
IL-1 beta (Pro-form) Monoclonal Antibody (NJTEN3), APC, eBioscience
catalog :
17-7114-80
quantity :
25 µg
price :
US 272
clonality :
monoclonal
host :
rat
conjugate :
APC
clone name :
NJTEN3
reactivity :
mouse
application :
ELISA, flow cytometry
more info or order :
citations: 48
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; fig 3b
Tian N, Hu L, Lu Y, Tong L, Feng M, Liu Q, et al. TKT maintains intestinal ATP production and inhibits apoptosis-induced colitis. Cell Death Dis. 2021;12:853 pubmed publisher
  • flow cytometry; mouse; 1:50; loading ...; fig s4b
Okamura T, Hashimoto Y, Mori J, Yamaguchi M, Majima S, Senmaru T, et al. ILC2s Improve Glucose Metabolism Through the Control of Saturated Fatty Acid Absorption Within Visceral Fat. Front Immunol. 2021;12:669629 pubmed publisher
  • flow cytometry; mouse; 1:65; loading ...; fig 6a
Glausen T, Carrillo G, Jin R, Boyle J, Saeij J, Wohlfert E, et al. The Toxoplasma Polymorphic Effector GRA15 Mediates Seizure Induction by Modulating Interleukin-1 Signaling in the Brain. MBio. 2021;12:e0133121 pubmed publisher
  • ELISA; mouse; fig 1c
Napolitano A, van der Veen A, Bunyan M, Borg A, Frith D, Howell S, et al. Cysteine-Reactive Free ISG15 Generates IL-1β-Producing CD8α+ Dendritic Cells at the Site of Infection. J Immunol. 2018;201:604-614 pubmed publisher
  • flow cytometry; mouse
Jovicic N, Jeftic I, Jovanovic I, Radosavljevic G, Arsenijevic N, Lukic M, et al. Differential Immunometabolic Phenotype in Th1 and Th2 Dominant Mouse Strains in Response to High-Fat Feeding. PLoS ONE. 2015;10:e0134089 pubmed publisher
Jeong G, Lee S, Kim D, Lyu J, Yoon G, Kim K, et al. Zika Virus Infection Induces Interleukin-1β-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model. J Virol. 2023;97:e0055623 pubmed publisher
Hegdekar N, Sarkar C, Bustos S, Ritzel R, Hanscom M, Ravishankar P, et al. Inhibition of autophagy in microglia and macrophages exacerbates innate immune responses and worsens brain injury outcomes. Autophagy. 2023;19:2026-2044 pubmed publisher
Boddupalli C, Nair S, Belinsky G, GANS J, Teeple E, Nguyen T, et al. Neuroinflammation in neuronopathic Gaucher disease: Role of microglia and NK cells, biomarkers, and response to substrate reduction therapy. elife. 2022;11: pubmed publisher
Zhao T, Liu S, Ding X, Johnson E, Hanna N, Singh K, et al. Lysosomal acid lipase, CSF1R, and PD-L1 determine functions of CD11c+ myeloid-derived suppressor cells. JCI Insight. 2022;7: pubmed publisher
Murray M, Crosby C, Marcovecchio P, Hartmann N, Chandra S, Zhao M, et al. Stimulation of a subset of natural killer T cells by CD103+ DC is required for GM-CSF and protection from pneumococcal infection. Cell Rep. 2022;38:110209 pubmed publisher
Zou L, He J, Gu L, Shahror R, Li Y, Cao T, et al. Brain innate immune response via miRNA-TLR7 sensing in polymicrobial sepsis. Brain Behav Immun. 2022;100:10-24 pubmed publisher
Luo Y, Guo J, Jia W, Wu M, Yin F, Niu G, et al. TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization. Oxid Med Cell Longev. 2021;2021:3877617 pubmed publisher
Kaur K, Bachus H, Lewis C, Papillion A, Rosenberg A, Ballesteros Tato A, et al. GM-CSF production by non-classical monocytes controls antagonistic LPS-driven functions in allergic inflammation. Cell Rep. 2021;37:110178 pubmed publisher
Formaglio P, Alabdullah M, Siokis A, Handschuh J, Sauerland I, Fu Y, et al. Nitric oxide controls proliferation of Leishmania major by inhibiting the recruitment of permissive host cells. Immunity. 2021;54:2724-2739.e10 pubmed publisher
Guo X, Xu X, Li T, Yu Q, Wang J, Chen Y, et al. NLRP3 Inflammasome Activation of Mast Cells by Estrogen via the Nuclear-Initiated Signaling Pathway Contributes to the Development of Endometriosis. Front Immunol. 2021;12:749979 pubmed publisher
Duan R, Yang C, Du T, Liu A, Wang A, Sun W, et al. Smek1 deficiency exacerbates experimental autoimmune encephalomyelitis by activating proinflammatory microglia and suppressing the IDO1-AhR pathway. J Neuroinflammation. 2021;18:145 pubmed publisher
Souza C, Ketelut Carneiro N, Milanezi C, Faccioli L, Gardinassi L, Silva J. NLRC4 inhibits NLRP3 inflammasome and abrogates effective antifungal CD8+ T cell responses. iScience. 2021;24:102548 pubmed publisher
Shao L, He Q, Wang J, He F, Lin S, Wu L, et al. MicroRNA-326 attenuates immune escape and prevents metastasis in lung adenocarcinoma by targeting PD-L1 and B7-H3. Cell Death Discov. 2021;7:145 pubmed publisher
Ratitong B, Marshall M, Pearlman E. β-Glucan-stimulated neutrophil secretion of IL-1α is independent of GSDMD and mediated through extracellular vesicles. Cell Rep. 2021;35:109139 pubmed publisher
Jones N, Blagih J, Zani F, Rees A, Hill D, Jenkins B, et al. Fructose reprogrammes glutamine-dependent oxidative metabolism to support LPS-induced inflammation. Nat Commun. 2021;12:1209 pubmed publisher
Ding L, Sontz E, Saqui Salces M, Merchant J. Interleukin-1β Suppresses Gastrin via Primary Cilia and Induces Antral Hyperplasia. Cell Mol Gastroenterol Hepatol. 2021;11:1251-1266 pubmed publisher
DeSouza Vieira T, Iniguez E, Serafim T, de Castro W, Karmakar S, Disotuar M, et al. Heme Oxygenase-1 Induction by Blood-Feeding Arthropods Controls Skin Inflammation and Promotes Disease Tolerance. Cell Rep. 2020;33:108317 pubmed publisher
Reverchon F, de Concini V, Larrigaldie V, Benmerzoug S, Briault S, Togbe D, et al. Hippocampal interleukin-33 mediates neuroinflammation-induced cognitive impairments. J Neuroinflammation. 2020;17:268 pubmed publisher
Liu X, Boyer M, Holmgren A, Shin S. Legionella-Infected Macrophages Engage the Alveolar Epithelium to Metabolically Reprogram Myeloid Cells and Promote Antibacterial Inflammation. Cell Host Microbe. 2020;28:683-698.e6 pubmed publisher
Castoldi A, Monteiro L, van Teijlingen Bakker N, Sanin D, Rana N, Corrado M, et al. Triacylglycerol synthesis enhances macrophage inflammatory function. Nat Commun. 2020;11:4107 pubmed publisher
Castro Dopico T, FLEMING A, Dennison T, Ferdinand J, Harcourt K, Stewart B, et al. GM-CSF Calibrates Macrophage Defense and Wound Healing Programs during Intestinal Infection and Inflammation. Cell Rep. 2020;32:107857 pubmed publisher
Rosser E, Piper C, Matei D, Blair P, Rendeiro A, Orford M, et al. Microbiota-Derived Metabolites Suppress Arthritis by Amplifying Aryl-Hydrocarbon Receptor Activation in Regulatory B Cells. Cell Metab. 2020;31:837-851.e10 pubmed publisher
Jain A, Irizarry Caro R, McDaniel M, Chawla A, Carroll K, Overcast G, et al. T cells instruct myeloid cells to produce inflammasome-independent IL-1β and cause autoimmunity. Nat Immunol. 2020;21:65-74 pubmed publisher
Leech J, Dhariwala M, Lowe M, Chu K, Merana G, Cornuot C, et al. Toxin-Triggered Interleukin-1 Receptor Signaling Enables Early-Life Discrimination of Pathogenic versus Commensal Skin Bacteria. Cell Host Microbe. 2019;26:795-809.e5 pubmed publisher
Castro Dopico T, Dennison T, Ferdinand J, Mathews R, FLEMING A, Clift D, et al. Anti-commensal IgG Drives Intestinal Inflammation and Type 17 Immunity in Ulcerative Colitis. Immunity. 2019;50:1099-1114.e10 pubmed publisher
Orr M, Khandhar A, Seydoux E, Liang H, Gage E, Mikasa T, et al. Reprogramming the adjuvant properties of aluminum oxyhydroxide with nanoparticle technology. NPJ Vaccines. 2019;4:1 pubmed publisher
Wasnik S, Rundle C, Baylink D, Yazdi M, Carreon E, Xu Y, et al. 1,25-Dihydroxyvitamin D suppresses M1 macrophages and promotes M2 differentiation at bone injury sites. JCI Insight. 2018;3: pubmed publisher
Gimblet C, Meisel J, Loesche M, Cole S, Horwinski J, Novais F, et al. Cutaneous Leishmaniasis Induces a Transmissible Dysbiotic Skin Microbiota that Promotes Skin Inflammation. Cell Host Microbe. 2017;22:13-24.e4 pubmed publisher
Kimball A, Joshi A, Boniakowski A, Schaller M, Chung J, ALLEN R, et al. Notch Regulates Macrophage-Mediated Inflammation in Diabetic Wound Healing. Front Immunol. 2017;8:635 pubmed publisher
Jin W, Shi S, Li Z, Li M, Wood K, Gonzales R, et al. Depletion of microglia exacerbates postischemic inflammation and brain injury. J Cereb Blood Flow Metab. 2017;37:2224-2236 pubmed publisher
Lucinda N, Figueiredo M, Pessoa N, Santos B, Lima G, Freitas A, et al. Dendritic cells, macrophages, NK and CD8+ T lymphocytes play pivotal roles in controlling HSV-1 in the trigeminal ganglia by producing IL1-beta, iNOS and granzyme B. Virol J. 2017;14:37 pubmed publisher
Ravindran R, Loebbermann J, Nakaya H, Khan N, Ma H, Gama L, et al. The amino acid sensor GCN2 controls gut inflammation by inhibiting inflammasome activation. Nature. 2016;531:523-527 pubmed publisher
Blaisdell A, Crequer A, Columbus D, Daikoku T, Mittal K, Dey S, et al. Neutrophils Oppose Uterine Epithelial Carcinogenesis via Debridement of Hypoxic Tumor Cells. Cancer Cell. 2015;28:785-799 pubmed publisher
Ray A, Castillo E, Morris K, Nofchissey R, Weston L, Samedi V, et al. Blockade of MK2 is protective in inflammation-associated colorectal cancer development. Int J Cancer. 2016;138:770-5 pubmed publisher
Berod L, Stüve P, Swallow M, Arnold Schrauf C, Kruse F, Gentilini M, et al. MyD88 signalling in myeloid cells is sufficient to prevent chronic mycobacterial infection. Eur J Immunol. 2014;44:1399-409 pubmed publisher
Sun B, Hu X, Liu G, Ma B, Xu Y, Yang T, et al. Phosphatase Wip1 negatively regulates neutrophil migration and inflammation. J Immunol. 2014;192:1184-95 pubmed publisher
Kim H, Lee H, Chang Y, Pichavant M, Shore S, Fitzgerald K, et al. Interleukin-17-producing innate lymphoid cells and the NLRP3 inflammasome facilitate obesity-associated airway hyperreactivity. Nat Med. 2014;20:54-61 pubmed publisher
Gower R, Boehler R, Azarin S, Ricci C, Leonard J, Shea L. Modulation of leukocyte infiltration and phenotype in microporous tissue engineering scaffolds via vector induced IL-10 expression. Biomaterials. 2014;35:2024-31 pubmed publisher
Enzler T, Sano Y, Choo M, Cottam H, Karin M, Tsao H, et al. Cell-selective inhibition of NF-?B signaling improves therapeutic index in a melanoma chemotherapy model. Cancer Discov. 2011;1:496-507 pubmed publisher
Mayer Barber K, Andrade B, Barber D, Hieny S, Feng C, Caspar P, et al. Innate and adaptive interferons suppress IL-1α and IL-1β production by distinct pulmonary myeloid subsets during Mycobacterium tuberculosis infection. Immunity. 2011;35:1023-34 pubmed publisher
Schroder K, Tschopp J. The inflammasomes. Cell. 2010;140:821-32 pubmed publisher
Becker C, Creagh E, O Neill L. Rab39a binds caspase-1 and is required for caspase-1-dependent interleukin-1beta secretion. J Biol Chem. 2009;284:34531-7 pubmed publisher
Rubartelli A, Cozzolino F, Talio M, Sitia R. A novel secretory pathway for interleukin-1 beta, a protein lacking a signal sequence. EMBO J. 1990;9:1503-10 pubmed
product information
Product Type :
Antibody
Product Name :
IL-1 beta (Pro-form) Monoclonal Antibody (NJTEN3), APC, eBioscience
Catalog # :
17-7114-80
Quantity :
25 µg
Price :
US 272
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.06 µg/test
Species :
Mouse
Clone :
NJTEN3
Isotype :
IgG1, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is instead secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions.IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. These cytokines play important roles in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. Their activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions, giving IL-RA or IL-1 beta neutralizing antibodies potential therapeutical value.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.06 µg/test
Aliases :
il 1b; IL 1ß; IL-1 beta; Il1b; Il-1b; IL-1beta; IL1ß; ILN; Interleukin; interleukin 1 beta; Interleukin1 beta; interleukin-1 beta; Interleukin-1b
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA