product summary
Loading...
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD326 (EpCAM) Monoclonal Antibody (G8.8), APC, eBioscience™
catalog :
17-5791-82
quantity :
100µg
price :
283.00 USD
clonality :
monoclonal
host :
rat
conjugate :
APC
clone name :
G8.8
reactivity :
mouse
application :
immunohistochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 63
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:200; loading ...
Jen H, Hill M, Tao L, Sheng K, Cao W, Zhang H, et al. Transcriptomic and epigenetic regulation of hair cell regeneration in the mouse utricle and its potentiation by Atoh1. elife. 2019;8: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5g
Lytle N, Ferguson L, Rajbhandari N, Gilroy K, Fox R, Deshpande A, et al. A Multiscale Map of the Stem Cell State in Pancreatic Adenocarcinoma. Cell. 2019;177:572-586.e22 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2f
  • flow cytometry; mouse; loading ...; fig s5a
Gronke K, Hernandez P, Zimmermann J, Klose C, Kofoed Branzk M, Guendel F, et al. Interleukin-22 protects intestinal stem cells against genotoxic stress. Nature. 2019;566:249-253 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 2b
Tammela T, Sanchez Rivera F, Cetinbas N, Wu K, Joshi N, Helenius K, et al. A Wnt-producing niche drives proliferative potential and progression in lung adenocarcinoma. Nature. 2017;545:355-359 pubmed publisher
  • flow cytometry; mouse; fig s4
Beyaz S, Mana M, Roper J, Kedrin D, Saadatpour A, Hong S, et al. High-fat diet enhances stemness and tumorigenicity of intestinal progenitors. Nature. 2016;531:53-8 pubmed publisher
  • immunohistochemistry; mouse; 0.5 ug/ml
Farin H, Jordens I, Mosa M, Basak O, Korving J, Tauriello D, et al. Visualization of a short-range Wnt gradient in the intestinal stem-cell niche. Nature. 2016;530:340-3 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 8d
Loyer X, Paradis V, Hénique C, Vion A, Colnot N, Guerin C, et al. Liver microRNA-21 is overexpressed in non-alcoholic steatohepatitis and contributes to the disease in experimental models by inhibiting PPARα expression. Gut. 2016;65:1882-1894 pubmed publisher
  • flow cytometry; mouse; 1:50
Causton B, Ramadas R, CHO J, Jones K, Pardo Saganta A, Rajagopal J, et al. CARMA3 Is Critical for the Initiation of Allergic Airway Inflammation. J Immunol. 2015;195:683-94 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 1
LUCAS B, White A, Ulvmar M, Nibbs R, Sitnik K, Agace W, et al. CCRL1/ACKR4 is expressed in key thymic microenvironments but is dispensable for T lymphopoiesis at steady state in adult mice. Eur J Immunol. 2015;45:574-83 pubmed publisher
  • flow cytometry; mouse
Venanzi E, Gray D, Benoist C, Mathis D. Lymphotoxin pathway and Aire influences on thymic medullary epithelial cells are unconnected. J Immunol. 2007;179:5693-700 pubmed
Erlangga Z, Wolff K, Poth T, Peltzer A, Nahnsen S, Spielberg S, et al. Potent Antitumor Activity of Liposomal Irinotecan in an Organoid- and CRISPR-Cas9-Based Murine Model of Gallbladder Cancer. Cancers (Basel). 2019;11: pubmed publisher
Wang J, Wang D, Chu K, Li W, Zeng Y. Procr-expressing progenitor cells are responsible for murine ovulatory rupture repair of ovarian surface epithelium. Nat Commun. 2019;10:4966 pubmed publisher
Pfefferle A, Darr D, Calhoun B, Mott K, Rosen J, Perou C. The MMTV-Wnt1 murine model produces two phenotypically distinct subtypes of mammary tumors with unique therapeutic responses to an EGFR inhibitor. Dis Model Mech. 2019;12: pubmed publisher
Saborowski A, Wolff K, Spielberg S, Beer B, Hartleben B, Erlangga Z, et al. Murine Liver Organoids as a Genetically Flexible System to Study Liver Cancer InVivo and In Vitro. Hepatol Commun. 2019;3:423-436 pubmed publisher
Glal D, Sudhakar J, Lu H, Liu M, Chiang H, Liu Y, et al. ATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases. Front Immunol. 2018;9:2522 pubmed publisher
Mao S, Park M, Cabrera R, Christin J, Karagiannis G, Oktay M, et al. Loss of amphiregulin reduces myoepithelial cell coverage of mammary ducts and alters breast tumor growth. Breast Cancer Res. 2018;20:131 pubmed publisher
Olabi S, Ucar A, Brennan K, Streuli C. Integrin-Rac signalling for mammary epithelial stem cell self-renewal. Breast Cancer Res. 2018;20:128 pubmed publisher
Moretti F, Klapproth S, Ruppert R, Margraf A, Weber J, Pick R, et al. Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus. elife. 2018;7: pubmed publisher
Bockerstett K, Wong C, Koehm S, Ford E, DiPaolo R. Molecular Characterization of Gastric Epithelial Cells Using Flow Cytometry. Int J Mol Sci. 2018;19: pubmed publisher
Lopes N, Charaix J, Cedile O, Serge A, Irla M. Lymphotoxin α fine-tunes T cell clonal deletion by regulating thymic entry of antigen-presenting cells. Nat Commun. 2018;9:1262 pubmed publisher
Yui S, Azzolin L, Maimets M, Pedersen M, Fordham R, Hansen S, et al. YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration. Cell Stem Cell. 2018;22:35-49.e7 pubmed publisher
Basova L, Tang X, Umasume T, Gromova A, Zyrianova T, Shmushkovich T, et al. Manipulation of Panx1 Activity Increases the Engraftment of Transplanted Lacrimal Gland Epithelial Progenitor Cells. Invest Ophthalmol Vis Sci. 2017;58:5654-5665 pubmed publisher
Entwistle L, Pelly V, Coomes S, Kannan Y, Perez Lloret J, Czieso S, et al. Epithelial-Cell-Derived Phospholipase A2 Group 1B Is an Endogenous Anthelmintic. Cell Host Microbe. 2017;22:484-493.e5 pubmed publisher
Henderson M, Madenspacher J, Whitehead G, Thomas S, Aloor J, Gowdy K, et al. Effects of Orally Ingested Arsenic on Respiratory Epithelial Permeability to Bacteria and Small Molecules in Mice. Environ Health Perspect. 2017;125:097024 pubmed publisher
Yu L, Chen S, Luo N, He S. The C-terminus domain of the hepatitis B virus x protein stimulates the proliferation of mouse foetal hepatic progenitor cells, although it is not required for the formation of spheroids. Int J Mol Med. 2017;40:400-410 pubmed publisher
Wu B, An C, Li Y, Yin Z, Gong L, Li Z, et al. Reconstructing Lineage Hierarchies of Mouse Uterus Epithelial Development Using Single-Cell Analysis. Stem Cell Reports. 2017;9:381-396 pubmed publisher
Brazee P, Soni P, Tokhtaeva E, Magnani N, Yemelyanov A, PERLMAN H, et al. FXYD5 Is an Essential Mediator of the Inflammatory Response during Lung Injury. Front Immunol. 2017;8:623 pubmed publisher
Herriges M, Tischfield D, Cui Z, Morley M, Han Y, Babu A, et al. The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis. Genes Dev. 2017;31:889-903 pubmed publisher
Roper J, Tammela T, Cetinbas N, Akkad A, Roghanian A, Rickelt S, et al. In vivo genome editing and organoid transplantation models of colorectal cancer and metastasis. Nat Biotechnol. 2017;35:569-576 pubmed publisher
Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, et al. DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs. Nature. 2017;543:568-572 pubmed publisher
Ohlund D, Handly Santana A, Biffi G, Elyada E, Almeida A, Ponz Sarvisé M, et al. Distinct populations of inflammatory fibroblasts and myofibroblasts in pancreatic cancer. J Exp Med. 2017;214:579-596 pubmed publisher
Sinha M, Lowell C. Isolation of Highly Pure Primary Mouse Alveolar Epithelial Type II Cells by Flow Cytometric Cell Sorting. Bio Protoc. 2016;6: pubmed publisher
Liu Z, Guo W, Zhang D, Pang Y, Shi J, Wan S, et al. Circulating tumor cell detection in hepatocellular carcinoma based on karyoplasmic ratios using imaging flow cytometry. Sci Rep. 2016;6:39808 pubmed publisher
Naikawadi R, Disayabutr S, Mallavia B, Donne M, Green G, La J, et al. Telomere dysfunction in alveolar epithelial cells causes lung remodeling and fibrosis. JCI Insight. 2016;1:e86704 pubmed publisher
Shi Y, Wu W, Chai Q, Li Q, Hou Y, Xia H, et al. LTβR controls thymic portal endothelial cells for haematopoietic progenitor cell homing and T-cell regeneration. Nat Commun. 2016;7:12369 pubmed publisher
Wang N, Dong B, Quan Y, Chen Q, Chu M, Xu J, et al. Regulation of Prostate Development and Benign Prostatic Hyperplasia by Autocrine Cholinergic Signaling via Maintaining the Epithelial Progenitor Cells in Proliferating Status. Stem Cell Reports. 2016;6:668-678 pubmed publisher
Lee C, Penberthy K, Wheeler K, Juncadella I, Vandenabeele P, Lysiak J, et al. Boosting Apoptotic Cell Clearance by Colonic Epithelial Cells Attenuates Inflammation In Vivo. Immunity. 2016;44:807-20 pubmed publisher
Maimets M, Rocchi C, Bron R, Pringle S, Kuipers J, Giepmans B, et al. Long-Term In Vitro Expansion of Salivary Gland Stem Cells Driven by Wnt Signals. Stem Cell Reports. 2016;6:150-62 pubmed publisher
Rios D, Wood M, Li J, Chassaing B, Gewirtz A, Williams I. Antigen sampling by intestinal M cells is the principal pathway initiating mucosal IgA production to commensal enteric bacteria. Mucosal Immunol. 2016;9:907-16 pubmed publisher
Pardo Saganta A, Tata P, Law B, Saez B, Chow R, Prabhu M, et al. Parent stem cells can serve as niches for their daughter cells. Nature. 2015;523:597-601 pubmed publisher
Williams B, Tebbutt N, Buchert M, Putoczki T, Doggett K, Bao S, et al. Glycoprotein A33 deficiency: a new mouse model of impaired intestinal epithelial barrier function and inflammatory disease. Dis Model Mech. 2015;8:805-15 pubmed publisher
Janeckova L, Pospichalova V, Fafilek B, Vojtechova M, Tureckova J, Dobes J, et al. HIC1 Tumor Suppressor Loss Potentiates TLR2/NF-κB Signaling and Promotes Tissue Damage-Associated Tumorigenesis. Mol Cancer Res. 2015;13:1139-48 pubmed publisher
Pospichalova V, Svoboda J, Dave Z, Kotrbova A, Kaiser K, Klemová D, et al. Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer. J Extracell Vesicles. 2015;4:25530 pubmed publisher
Lina T, Alzahrani S, House J, Yamaoka Y, Sharpe A, Rampy B, et al. Helicobacter pylori cag pathogenicity island's role in B7-H1 induction and immune evasion. PLoS ONE. 2015;10:e0121841 pubmed publisher
Clatworthy M, Aronin C, Mathews R, Morgan N, Smith K, Germain R. Immune complexes stimulate CCR7-dependent dendritic cell migration to lymph nodes. Nat Med. 2014;20:1458-63 pubmed publisher
Walmsley G, Rinkevich Y, Hu M, Montoro D, Lo D, McArdle A, et al. Live fibroblast harvest reveals surface marker shift in vitro. Tissue Eng Part C Methods. 2015;21:314-21 pubmed publisher
Zhang K, Dupont A, Torow N, Gohde F, Gohde F, Leschner S, et al. Age-dependent enterocyte invasion and microcolony formation by Salmonella. PLoS Pathog. 2014;10:e1004385 pubmed publisher
White A, Jenkinson W, Cowan J, Parnell S, Bacon A, Jones N, et al. An essential role for medullary thymic epithelial cells during the intrathymic development of invariant NKT cells. J Immunol. 2014;192:2659-66 pubmed publisher
Tata P, Mou H, Pardo Saganta A, Zhao R, Prabhu M, Law B, et al. Dedifferentiation of committed epithelial cells into stem cells in vivo. Nature. 2013;503:218-23 pubmed publisher
Alenghat T, Osborne L, Saenz S, Kobuley D, Ziegler C, Mullican S, et al. Histone deacetylase 3 coordinates commensal-bacteria-dependent intestinal homeostasis. Nature. 2013;504:153-7 pubmed publisher
Babic A, Jang S, Nicolov E, Voicu H, Luckey C. Culture of mouse amniotic fluid-derived cells on irradiated STO feeders results in the generation of primitive endoderm cell lines capable of self-renewal in vitro. Cells Tissues Organs. 2013;198:111-26 pubmed publisher
Lina T, Pinchuk I, House J, Yamaoka Y, Graham D, Beswick E, et al. CagA-dependent downregulation of B7-H2 expression on gastric mucosa and inhibition of Th17 responses during Helicobacter pylori infection. J Immunol. 2013;191:3838-46 pubmed publisher
Magness S, Puthoff B, Crissey M, Dunn J, Henning S, Houchen C, et al. A multicenter study to standardize reporting and analyses of fluorescence-activated cell-sorted murine intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2013;305:G542-51 pubmed publisher
Reichert M, Takano S, Heeg S, Bakir B, Botta G, Rustgi A. Isolation, culture and genetic manipulation of mouse pancreatic ductal cells. Nat Protoc. 2013;8:1354-65 pubmed publisher
Goldman O, Han S, Sourisseau M, Sourrisseau M, Dziedzic N, Hamou W, et al. KDR identifies a conserved human and murine hepatic progenitor and instructs early liver development. Cell Stem Cell. 2013;12:748-60 pubmed publisher
El Zaatari M, Kao J, Tessier A, Bai L, Hayes M, Fontaine C, et al. Gli1 deletion prevents Helicobacter-induced gastric metaplasia and expansion of myeloid cell subsets. PLoS ONE. 2013;8:e58935 pubmed publisher
Baik S, Jenkinson E, Lane P, Anderson G, Jenkinson W. Generation of both cortical and Aire(+) medullary thymic epithelial compartments from CD205(+) progenitors. Eur J Immunol. 2013;43:589-94 pubmed publisher
Yilmaz O, Katajisto P, Lamming D, Gültekin Y, Bauer Rowe K, Sengupta S, et al. mTORC1 in the Paneth cell niche couples intestinal stem-cell function to calorie intake. Nature. 2012;486:490-5 pubmed publisher
Cook B, Liu S, Evans T. Smad1 signaling restricts hematopoietic potential after promoting hemangioblast commitment. Blood. 2011;117:6489-97 pubmed publisher
Dooley J, Erickson M, Farr A. An organized medullary epithelial structure in the normal thymus expresses molecules of respiratory epithelium and resembles the epithelial thymic rudiment of nude mice. J Immunol. 2005;175:4331-7 pubmed
Nelson A, Dunn R, Peach R, Aruffo A, Farr A. The murine homolog of human Ep-CAM, a homotypic adhesion molecule, is expressed by thymocytes and thymic epithelial cells. Eur J Immunol. 1996;26:401-8 pubmed
Borkowski T, Nelson A, Farr A, Udey M. Expression of gp40, the murine homologue of human epithelial cell adhesion molecule (Ep-CAM), by murine dendritic cells. Eur J Immunol. 1996;26:110-4 pubmed
Farr A, Nelson A, Truex J, Hosier S. Epithelial heterogeneity in the murine thymus: a cell surface glycoprotein expressed by subcapsular and medullary epithelium. J Histochem Cytochem. 1991;39:645-53 pubmed
product information
Product Type :
Antibody
Product Name :
CD326 (EpCAM) Monoclonal Antibody (G8.8), APC, eBioscience™
Catalog # :
17-5791-82
Quantity :
100µg
Price (USD) :
283.00 USD
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.125 µg/test
Species :
Mouse
Clone :
G8.8
Isotype :
IgG2a, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.125 µg/test
Aliases :
adenocarcinoma-associated antigen; CD326; Cell surface glycoprotein Trop-1; DIAR5; EGP; EGP-2; EGP314; EGP40; Epcam; Ep-CAM; EpCAM1; Epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; Epithelial glycoprotein 314; ESA; GA733-2; gp40; hEGP314; HNPCC8; human epithelial glycoprotein-2; KS 1/4 antigen; KS1/4; KSA; Ly74; lymphocyte antigen 74; M1S2; M4S1; Major gastrointestinal tumor-associated protein GA733-2; mEGP314; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; panepithelial glycoprotein 314; protein 289A; Protein D5.7A; Tacsd1; Tacstd1; TROP1; Trop-1 protein; Tumor-associated calcium signal transducer 1
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA