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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), APC, eBioscience™
catalog :
17-5321-81
quantity :
50 µg
price :
US 309.00
clonality :
monoclonal
host :
rat
conjugate :
APC
clone name :
M5/114.15.2
reactivity :
human, mouse
application :
immunocytochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 103
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:1200; loading ...; fig 4s1
Guyon C, Jmari N, Padonou F, Li Y, Ucar O, Fujikado N, et al. Aire-dependent genes undergo Clp1-mediated 3'UTR shortening associated with higher transcript stability in the thymus. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:400; fig s3
Szalay G, Martinecz B, Lénárt N, Kornyei Z, Orsolits B, Judák L, et al. Microglia protect against brain injury and their selective elimination dysregulates neuronal network activity after stroke. Nat Commun. 2016;7:11499 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig s3
Hanot Mambres D, Machelart A, Vanderwinden J, De Trez C, Ryffel B, Letesson J, et al. In Situ Characterization of Splenic Brucella melitensis Reservoir Cells during the Chronic Phase of Infection in Susceptible Mice. PLoS ONE. 2015;10:e0137835 pubmed publisher
  • flow cytometry; mouse; fig 2
Dewas C, Chen X, Honda T, Junttila I, Linton J, Udey M, et al. TSLP expression: analysis with a ZsGreen TSLP reporter mouse. J Immunol. 2015;194:1372-80 pubmed publisher
  • flow cytometry; mouse
Weber B, Schuster S, Zysset D, Rihs S, Dickgreber N, Schürch C, et al. TREM-1 deficiency can attenuate disease severity without affecting pathogen clearance. PLoS Pathog. 2014;10:e1003900 pubmed publisher
  • flow cytometry; human
Botelho F, Bauer C, Finch D, Nikota J, Zavitz C, Kelly A, et al. IL-1?/IL-1R1 expression in chronic obstructive pulmonary disease and mechanistic relevance to smoke-induced neutrophilia in mice. PLoS ONE. 2011;6:e28457 pubmed publisher
  • flow cytometry; mouse; fig 1
  • immunocytochemistry; mouse; fig 7
Charles E, Joshi S, Ash J, Fox B, Farris A, Bzik D, et al. CD4 T-cell suppression by cells from Toxoplasma gondii-infected retinas is mediated by surface protein PD-L1. Infect Immun. 2010;78:3484-92 pubmed publisher
  • flow cytometry; mouse; tbl 1
Zavitz C, Bauer C, Gaschler G, Fraser K, Strieter R, Hogaboam C, et al. Dysregulated macrophage-inflammatory protein-2 expression drives illness in bacterial superinfection of influenza. J Immunol. 2010;184:2001-13 pubmed publisher
Wang L, Li Y, Wang X, Wang P, Essandoh K, Cui S, et al. GDF3 Protects Mice against Sepsis-Induced Cardiac Dysfunction and Mortality by Suppression of Macrophage Pro-Inflammatory Phenotype. Cells. 2020;9: pubmed publisher
Dokoshi T, Zhang L, Li F, Nakatsuji T, Butcher A, Yoshida H, et al. Hyaluronan Degradation by Cemip Regulates Host Defense against Staphylococcus aureus Skin Infection. Cell Rep. 2020;30:61-68.e4 pubmed publisher
Fang D, Tang Q, Kong Y, Wang Q, Gu J, Fang X, et al. MDM2 inhibitor APG-115 synergizes with PD-1 blockade through enhancing antitumor immunity in the tumor microenvironment. J Immunother Cancer. 2019;7:327 pubmed publisher
Sheng W, Chauhan P, Hu S, Prasad S, Lokensgard J. Antiallodynic Effects of Cannabinoid Receptor 2 (CB2R) Agonists on Retrovirus Infection-Induced Neuropathic Pain. Pain Res Manag. 2019;2019:1260353 pubmed publisher
Ramakrishna C, Kujawski M, Chu H, Li L, Mazmanian S, CANTIN E. Bacteroides fragilis polysaccharide A induces IL-10 secreting B and T cells that prevent viral encephalitis. Nat Commun. 2019;10:2153 pubmed publisher
Duan J, Lu G, Hong Y, Hu Q, Mai X, Guo J, et al. Live imaging and tracking of genome regions in CRISPR/dCas9 knock-in mice. Genome Biol. 2018;19:192 pubmed publisher
Flores C, Wildes T, Drake J, Moore G, Dean B, Abraham R, et al. Lin-CCR2+ hematopoietic stem and progenitor cells overcome resistance to PD-1 blockade. Nat Commun. 2018;9:4313 pubmed publisher
Buxade M, Huerga Encabo H, Riera Borrull M, Quintana Gallardo L, López Cotarelo P, Tellechea M, et al. Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5. J Exp Med. 2018;215:2901-2918 pubmed publisher
Gralinski L, Sheahan T, Morrison T, Menachery V, Jensen K, Leist S, et al. Complement Activation Contributes to Severe Acute Respiratory Syndrome Coronavirus Pathogenesis. MBio. 2018;9: pubmed publisher
Zhou Y, Liu F, Li C, Shi G, Xu X, Luo X, et al. Construction and Characterization of Adenovirus Vectors Encoding Aspartate-β-Hydroxylase to Preliminary Application in Immunotherapy of Hepatocellular Carcinoma. J Immunol Res. 2018;2018:9832467 pubmed publisher
Bonacina F, Coe D, Wang G, Longhi M, Baragetti A, Moregola A, et al. Myeloid apolipoprotein E controls dendritic cell antigen presentation and T cell activation. Nat Commun. 2018;9:3083 pubmed publisher
Gyori D, Lim E, Grant F, Spensberger D, Roychoudhuri R, Shuttleworth S, et al. Compensation between CSF1R+ macrophages and Foxp3+ Treg cells drives resistance to tumor immunotherapy. JCI Insight. 2018;3: pubmed publisher
García Nores G, Ly C, Cuzzone D, Kataru R, Hespe G, Torrisi J, et al. CD4+ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema. Nat Commun. 2018;9:1970 pubmed publisher
Takamori A, Nambu A, Sato K, Yamaguchi S, Matsuda K, Numata T, et al. IL-31 is crucial for induction of pruritus, but not inflammation, in contact hypersensitivity. Sci Rep. 2018;8:6639 pubmed publisher
Xu M, Lu H, Lee Y, Wu Y, Liu K, Shi Y, et al. An Interleukin-25-Mediated Autoregulatory Circuit in Keratinocytes Plays a Pivotal Role in Psoriatic Skin Inflammation. Immunity. 2018;48:787-798.e4 pubmed publisher
Li J, Lee Y, Li Y, Jiang Y, Lu H, Zang W, et al. Co-inhibitory Molecule B7 Superfamily Member 1 Expressed by Tumor-Infiltrating Myeloid Cells Induces Dysfunction of Anti-tumor CD8+ T Cells. Immunity. 2018;48:773-786.e5 pubmed publisher
Nabe T, Matsuda M, Ishida T, Tsujimoto N, Kido H, Kanaya H, et al. Antigen-specific airway IL-33 production depends on FcγR-mediated incorporation of the antigen by alveolar macrophages in sensitized mice. Immunology. 2018;155:99-111 pubmed publisher
Geslewitz W, Percopo C, Rosenberg H. FACS isolation of live mouse eosinophils at high purity via a protocol that does not target Siglec F. J Immunol Methods. 2018;454:27-31 pubmed publisher
Gaya M, Barral P, Burbage M, Aggarwal S, Montaner B, Warren Navia A, et al. Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells. Cell. 2018;172:517-533.e20 pubmed publisher
Micanovic R, Khan S, Janosevic D, Lee M, Hato T, Srour E, et al. Tamm-Horsfall Protein Regulates Mononuclear Phagocytes in the Kidney. J Am Soc Nephrol. 2018;29:841-856 pubmed publisher
Bai W, Li C, Zhang C, Xu L, Zeng Q, Hu B, et al. Prolonged Deleterious Influences of Chemotherapeutic Agent CPT-11 on Resident Peritoneal Macrophages and B1 Cells. Front Immunol. 2017;8:1919 pubmed publisher
Wang J, Dai X, Hsu C, Ming C, He Y, Zhang J, et al. Discrimination of the heterogeneity of bone marrow‑derived dendritic cells. Mol Med Rep. 2017;16:6787-6793 pubmed publisher
Cruz S, Hari A, Qin Z, Couture P, Huang H, Lagace D, et al. Loss of IRF2BP2 in Microglia Increases Inflammation and Functional Deficits after Focal Ischemic Brain Injury. Front Cell Neurosci. 2017;11:201 pubmed publisher
Magnuson A, Regan D, Fouts J, Booth A, Dow S, Foster M. Diet-induced obesity causes visceral, but not subcutaneous, lymph node hyperplasia via increases in specific immune cell populations. Cell Prolif. 2017;50: pubmed publisher
McDonald L, Johnson S, Russell D, Young M, LaRue A. Role of a novel immune modulating DDR2-expressing population in silica-induced pulmonary fibrosis. PLoS ONE. 2017;12:e0180724 pubmed publisher
Macpherson K, Sompol P, Kannarkat G, Chang J, Sniffen L, Wildner M, et al. Peripheral administration of the soluble TNF inhibitor XPro1595 modifies brain immune cell profiles, decreases beta-amyloid plaque load, and rescues impaired long-term potentiation in 5xFAD mice. Neurobiol Dis. 2017;102:81-95 pubmed publisher
Berlato C, Khan M, Schioppa T, Thompson R, Maniati E, Montfort A, et al. A CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer. J Clin Invest. 2017;127:801-813 pubmed publisher
Wang H, Hu W, Liang Z, Zeng L, Li J, Yan H, et al. Thiol peroxiredoxin, a novel allergen from Bombyx mori, modulates functions of macrophages and dendritic cells. Am J Transl Res. 2016;8:5320-5329 pubmed
Habener A, Behrendt A, Skuljec J, Jirmo A, Meyer Bahlburg A, Hansen G. B cell subsets are modulated during allergic airway inflammation but are not required for the development of respiratory tolerance in a murine model. Eur J Immunol. 2017;47:552-562 pubmed publisher
Moderzynski K, Papp S, Rauch J, Heine L, Kuehl S, Richardt U, et al. CD4+ T Cells Are as Protective as CD8+ T Cells against Rickettsia typhi Infection by Activating Macrophage Bactericidal Activity. PLoS Negl Trop Dis. 2016;10:e0005089 pubmed publisher
Perobelli S, Mercadante A, Galvani R, Gonçalves Silva T, Alves A, Pereira Neves A, et al. G-CSF-Induced Suppressor IL-10+ Neutrophils Promote Regulatory T Cells That Inhibit Graft-Versus-Host Disease in a Long-Lasting and Specific Way. J Immunol. 2016;197:3725-3734 pubmed
Raguz J, Jerić I, Niault T, Nowacka J, Kuzet S, Rupp C, et al. Epidermal RAF prevents allergic skin disease. elife. 2016;5: pubmed publisher
McCullough L, Mirza M, Xu Y, Bentivegna K, Steffens E, Ritzel R, et al. Stroke sensitivity in the aged: sex chromosome complement vs. gonadal hormones. Aging (Albany NY). 2016;8:1432-41 pubmed publisher
Menendez C, Jinkins J, Carr D. Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency. J Immunol. 2016;197:1262-75 pubmed publisher
Zheng H, Zou W, Shen J, Xu L, Wang S, Fu Y, et al. Opposite Effects of Coinjection and Distant Injection of Mesenchymal Stem Cells on Breast Tumor Cell Growth. Stem Cells Transl Med. 2016;5:1216-28 pubmed publisher
Lopes Pinheiro M, Kamermans A, Garcia Vallejo J, Van het Hof B, Wierts L, O Toole T, et al. Internalization and presentation of myelin antigens by the brain endothelium guides antigen-specific T cell migration. elife. 2016;5: pubmed publisher
Cao Y, Feng Y, Zhang Y, Zhu X, Jin F. L-Arginine supplementation inhibits the growth of breast cancer by enhancing innate and adaptive immune responses mediated by suppression of MDSCs in vivo. BMC Cancer. 2016;16:343 pubmed publisher
Bracchi Ricard V, Zha J, Smith A, Lopez Rodriguez D, Bethea J, Andreansky S. Chronic spinal cord injury attenuates influenza virus-specific antiviral immunity. J Neuroinflammation. 2016;13:125 pubmed publisher
Xu X, Gao Y, Zhai Z, Zhang S, Shan F, Feng J. Repulsive guidance molecule a blockade exerts the immunoregulatory function in DCs stimulated with ABP and LPS. Hum Vaccin Immunother. 2016;12:2169-2180 pubmed
Xiao J, Shao L, Shen J, Jiang W, Feng Y, Zheng P, et al. Effects of ketanserin on experimental colitis in mice and macrophage function. Int J Mol Med. 2016;37:659-68 pubmed publisher
Grabert K, Michoel T, Karavolos M, Clohisey S, Baillie J, Stevens M, et al. Microglial brain region-dependent diversity and selective regional sensitivities to aging. Nat Neurosci. 2016;19:504-16 pubmed publisher
Dudek M, Puttur F, Arnold Schrauf C, Kühl A, Holzmann B, Henriques Normark B, et al. Lung epithelium and myeloid cells cooperate to clear acute pneumococcal infection. Mucosal Immunol. 2016;9:1288-302 pubmed publisher
Lagassé H, Anidi I, Craig J, Limjunyawong N, Poupore A, Mitzner W, et al. Recruited monocytes modulate malaria-induced lung injury through CD36-mediated clearance of sequestered infected erythrocytes. J Leukoc Biol. 2016;99:659-71 pubmed publisher
Plaks V, Boldajipour B, Linnemann J, Nguyen N, Kersten K, Wolf Y, et al. Adaptive Immune Regulation of Mammary Postnatal Organogenesis. Dev Cell. 2015;34:493-504 pubmed publisher
DeFalco T, Potter S, Williams A, Waller B, Kan M, Capel B. Macrophages Contribute to the Spermatogonial Niche in the Adult Testis. Cell Rep. 2015;12:1107-19 pubmed publisher
Huang L, Kong Y, Wang J, Sun J, Shi Q, Qiu Y. Reducing progression of experimental lupus nephritis via inhibition of the B7/CD28 signaling pathway. Mol Med Rep. 2015;12:4187-4195 pubmed publisher
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Boulay A, Mazeraud A, Cisternino S, Saubaméa B, Mailly P, Jourdren L, et al. Immune quiescence of the brain is set by astroglial connexin 43. J Neurosci. 2015;35:4427-39 pubmed publisher
Bourke C, Prendergast C, Sanin D, Oulton T, Hall R, Mountford A. Epidermal keratinocytes initiate wound healing and pro-inflammatory immune responses following percutaneous schistosome infection. Int J Parasitol. 2015;45:215-24 pubmed publisher
Buela K, Hendricks R. Cornea-infiltrating and lymph node dendritic cells contribute to CD4+ T cell expansion after herpes simplex virus-1 ocular infection. J Immunol. 2015;194:379-87 pubmed publisher
Zonneveld M, Brisson A, van Herwijnen M, Tan S, van de Lest C, Redegeld F, et al. Recovery of extracellular vesicles from human breast milk is influenced by sample collection and vesicle isolation procedures. J Extracell Vesicles. 2014;3: pubmed publisher
Xiao G, Liu I, Cheng C, Chang C, Lee Y, Cheng W, et al. Amniotic fluid stem cells prevent follicle atresia and rescue fertility of mice with premature ovarian failure induced by chemotherapy. PLoS ONE. 2014;9:e106538 pubmed publisher
Schulz M, Atay C, Heringer J, Romrig F, Schwitalla S, Aydin B, et al. High-fat-diet-mediated dysbiosis promotes intestinal carcinogenesis independently of obesity. Nature. 2014;514:508-12 pubmed publisher
Neerincx A, Jakobshagen K, Utermöhlen O, Buning H, Steimle V, Kufer T. The N-terminal domain of NLRC5 confers transcriptional activity for MHC class I and II gene expression. J Immunol. 2014;193:3090-100 pubmed publisher
Sparber F, Tripp C, Komenda K, Scheffler J, Clausen B, Huber L, et al. The late endosomal adaptor molecule p14 (LAMTOR2) regulates TGF?1-mediated homeostasis of Langerhans cells. J Invest Dermatol. 2015;135:119-129 pubmed publisher
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Ahn D, Parker D, Planet P, Nieto P, Bueno S, Prince A. Secretion of IL-16 through TNFR1 and calpain-caspase signaling contributes to MRSA pneumonia. Mucosal Immunol. 2014;7:1366-74 pubmed publisher
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Bakhru P, Sirisaengtaksin N, Soudani E, Mukherjee S, Khan A, Jagannath C. BCG vaccine mediated reduction in the MHC-II expression of macrophages and dendritic cells is reversed by activation of Toll-like receptors 7 and 9. Cell Immunol. 2014;287:53-61 pubmed publisher
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Roehrich M, Spicher A, Milano G, Vassalli G. Characterization of cardiac-resident progenitor cells expressing high aldehyde dehydrogenase activity. Biomed Res Int. 2013;2013:503047 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), APC, eBioscience™
Catalog # :
17-5321-81
Quantity :
50 µg
Price :
US 309.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.03 µg/test
Species :
Mouse
Clone :
M5/114.15.2
Isotype :
IgG2b, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.03 µg/test
Aliases :
Abeta; AI323765; AI845868; cell surface glycoprotein; E-alpha-f; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; H-2Ab; H2-Ab; H2-Ab1; H-2Ea; H2-Ea; H2-Ea-ps; H2Eb; H-2Eb; H2-Eb1; H2-iabeta; H2-IE-alpha; histocompatibility 2, class II antigen A, beta 1; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; histocompatibility 2, class II antigen E beta; Ia2; Ia-2; Ia3; Ia-3; Ia4; Ia-4; IAb; I-Ab; I-Abeta; I-Ad; I-Aq; I-E alpha MHC class II; I-E beta MHC class II; I-Ealpha; I-Ed; I-Ek; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; MHC class 2; MHC class II antigen A beta; MHC class II antigen E alpha; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC class II protein; MHC H2-IE-beta cell surface glycoprotein; response to metastatic cancers 1; Rmcs1
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA