CD172a (SIRP alpha) Monoclonal Antibody (15-414), APC, eBioscience | Invitrogen 17-1729-42 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD172a (SIRP alpha) Monoclonal Antibody (15-414), APC, eBioscience

catalog :

17-1729-42

quantity :

100 Tests

price :

US 317

clonality :

monoclonal

host :

mouse

conjugate :

APC

clone name :

15-414

reactivity :

human

application :

flow cytometry

more info or order :

Invitrogen product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:50; fig 3a, 3b	Menon V, Thomas R, Elgueta C, Horl M, Osborn T, Hallett P, et al. Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells. Stem Cells. 2019;37:1293-1306 pubmed publisher
flow cytometry; human	Balan S, Ollion V, Colletti N, Chelbi R, Montanana Sanchis F, Liu H, et al. Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells. J Immunol. 2014;193:1622-35 pubmed publisher
	Hansen I, Krabbendam L, Bernink J, Loayza Puch F, Hoepel W, van Burgsteden J, et al. FcαRI co-stimulation converts human intestinal CD103+ dendritic cells into pro-inflammatory cells through glycolytic reprogramming. Nat Commun. 2018;9:863 pubmed publisher
	Berry M, Mathews R, Ferdinand J, Jing C, Loudon K, Wlodek E, et al. Renal Sodium Gradient Orchestrates a Dynamic Antibacterial Defense Zone. Cell. 2017;170:860-874.e19 pubmed publisher
	Serra Hassoun M, Bourgine M, Boniotto M, Berges J, Langa F, Michel M, et al. Human hematopoietic reconstitution and HLA-restricted responses in nonpermissive alymphoid mice. J Immunol. 2014;193:1504-11 pubmed publisher
	Abreu M, Sealy L. Cells expressing the C/EBPbeta isoform, LIP, engulf their neighbors. PLoS ONE. 2012;7:e41807 pubmed publisher
	Barclay A. Signal regulatory protein alpha (SIRPalpha)/CD47 interaction and function. Curr Opin Immunol. 2009;21:47-52 pubmed publisher
	Liu Y, Buhring H, Zen K, Burst S, Schnell F, Williams I, et al. Signal regulatory protein (SIRPalpha), a cellular ligand for CD47, regulates neutrophil transmigration. J Biol Chem. 2002;277:10028-36 pubmed
	Seiffert M, Brossart P, Cant C, Cella M, Colonna M, Brugger W, et al. Signal-regulatory protein alpha (SIRPalpha) but not SIRPbeta is involved in T-cell activation, binds to CD47 with high affinity, and is expressed on immature CD34(+)CD38(-) hematopoietic cells. Blood. 2001;97:2741-9 pubmed

product information

Product Type :

Antibody

Product Name :

CD172a (SIRP alpha) Monoclonal Antibody (15-414), APC, eBioscience

Catalog # :

17-1729-42

Quantity :

100 Tests

Price :

US 317

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 5 µL (0.5 µg)/test

Species :

Human

Clone :

15-414

Isotype :

IgG2a, kappa

Storage :

4° C, store in dark, DO NOT FREEZE!

Description :

SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and hematopoietic stem cells. SIRP alpha acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. The ligand binding of SIRP alpha to integrin-associated protein CD47 results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of SIRP alpha, which mediates the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. Ligation of SIRP alpha with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs. SIRP alpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion, and plays a general role in different physiological and pathological processes. Cancer cells highly express CD47, which activates SIRP alpha and inhibits macrophage-mediated destruction of cancerous cell growth.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 5 µL (0.5 µg)/test

Aliases :

AI835480; Bit; brain Ig-like molecule with tyrosine-based activation motifs; Brain immunoglobulin like protein with tyrosine - based activation motifs; brain immunological-like with tyrosine-based motifs; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; CD172; CD172 alpha; CD172 antigen-like family member A; CD172A; Inhibitory receptor SHPS-1; macrophage fusion receptor; Macrophage membrane protein MFP150; MFR; mSIRP-alpha1; Myd1; MYD-1; myD-1 antigen; p84; Protein tyrosine phosphatase non-receptor type substrate 1 (SHP substrate 1); protein tyrosine phosphatase, non-receptor type substrate 1; Protein tyrosine phosphatase, non-receptor type substrate 1 (SHP substrate 1); Ptpns1; SHP substrate 1; SHP-1; SHPS1; SHPS-1; signal regulatory protein alpha; signal-regulatory protein alpha; signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; SIRP; Sirpa; SIRPalpha; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3; swc3; swine workshop cluster 3 antigen; swine workshop cluster 3 antigen precursor; tyrosine phosphatase SHP substrate 1; tyrosine-protein phosphatase non-receptor type substrate 1

more info or order :

Invitrogen product webpage