product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD135 (Flt3) Monoclonal Antibody (A2F10), APC, eBioscience
catalog :
17-1351-80
quantity :
25 µg
price :
US 102
clonality :
monoclonal
host :
rat
conjugate :
APC
clone name :
A2F10
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 65
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...
Ricci B, Tycksen E, Celik H, Belle J, Fontana F, Civitelli R, et al. Osterix-Cre marks distinct subsets of CD45- and CD45+ stromal populations in extra-skeletal tumors with pro-tumorigenic characteristics. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...
Cole C, Verdoni A, Ketkar S, Leight E, Russler Germain D, Lamprecht T, et al. PML-RARA requires DNA methyltransferase 3A to initiate acute promyelocytic leukemia. J Clin Invest. 2016;126:85-98 pubmed publisher
Naka K, Ochiai R, Matsubara E, Kondo C, Yang K, Hoshii T, et al. The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells. Nat Commun. 2020;11:4681 pubmed publisher
Morganti C, Bonora M, Ito K. Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps. J Vis Exp. 2019;: pubmed publisher
Walker J, Clark P, Crisp A, Barlow J, Szeto A, Ferreira A, et al. Polychromic Reporter Mice Reveal Unappreciated Innate Lymphoid Cell Progenitor Heterogeneity and Elusive ILC3 Progenitors in Bone Marrow. Immunity. 2019;: pubmed publisher
Xu W, Cherrier D, Chea S, Vosshenrich C, Serafini N, Petit M, et al. An Id2RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials. Immunity. 2019;50:1054-1068.e3 pubmed publisher
Yamamoto R, Wilkinson A, Ooehara J, Lan X, Lai C, Nakauchi Y, et al. Large-Scale Clonal Analysis Resolves Aging of the Mouse Hematopoietic Stem Cell Compartment. Cell Stem Cell. 2018;22:600-607.e4 pubmed publisher
Miyazaki M, Miyazaki K, Chen K, Jin Y, Turner J, Moore A, et al. The E-Id Protein Axis Specifies Adaptive Lymphoid Cell Identity and Suppresses Thymic Innate Lymphoid Cell Development. Immunity. 2017;46:818-834.e4 pubmed publisher
Fang J, Bolanos L, Choi K, Liu X, Christie S, Akunuru S, et al. Ubiquitination of hnRNPA1 by TRAF6 links chronic innate immune signaling with myelodysplasia. Nat Immunol. 2017;18:236-245 pubmed publisher
Zhu N, Chen M, Eng R, DeJong J, Sinha A, Rahnamay N, et al. MLL-AF9- and HOXA9-mediated acute myeloid leukemia stem cell self-renewal requires JMJD1C. J Clin Invest. 2016;126:997-1011 pubmed publisher
Siu K, Xu Y, Swartz K, Bhattacharyya M, Gurbuxani S, Hua Y, et al. Chromosome instability underlies hematopoietic stem cell dysfunction and lymphoid neoplasia associated with impaired Fbw7-mediated cyclin E regulation. Mol Cell Biol. 2014;34:3244-58 pubmed publisher
Li J, Razumilava N, Gores G, Walters S, Mizuochi T, Mourya R, et al. Biliary repair and carcinogenesis are mediated by IL-33-dependent cholangiocyte proliferation. J Clin Invest. 2014;124:3241-51 pubmed publisher
Wong T, Doyle A, Lee J, Jelinek D. Eosinophils regulate peripheral B cell numbers in both mice and humans. J Immunol. 2014;192:3548-58 pubmed publisher
Chen Y, Chen T, Pai L, Wesoly J, Bluyssen H, Lee C. A type I IFN-Flt3 ligand axis augments plasmacytoid dendritic cell development from common lymphoid progenitors. J Exp Med. 2013;210:2515-22 pubmed publisher
Campaner S, Viale A, De Fazio S, Doni M, De Franco F, D Artista L, et al. A non-redundant function of cyclin E1 in hematopoietic stem cells. Cell Cycle. 2013;12:3663-72 pubmed publisher
Maina V, Marrella V, Mantero S, Cassani B, Fontana E, Anselmo A, et al. Hypomorphic mutation in the RAG2 gene affects dendritic cell distribution and migration. J Leukoc Biol. 2013;94:1221-30 pubmed publisher
Adorno M, Sikandar S, Mitra S, Kuo A, Nicolis Di Robilant B, Haro Acosta V, et al. Usp16 contributes to somatic stem-cell defects in Down's syndrome. Nature. 2013;501:380-4 pubmed publisher
Kawakami T, Lichtnekert J, Thompson L, Karna P, Bouabe H, Hohl T, et al. Resident renal mononuclear phagocytes comprise five discrete populations with distinct phenotypes and functions. J Immunol. 2013;191:3358-72 pubmed publisher
Singh R, Franke K, Kalucka J, Mamlouk S, Muschter A, Gembarska A, et al. HIF prolyl hydroxylase 2 (PHD2) is a critical regulator of hematopoietic stem cell maintenance during steady-state and stress. Blood. 2013;121:5158-66 pubmed publisher
Michel A, Schuler A, Friedrich P, Döner F, Bopp T, Radsak M, et al. Mast cell-deficient Kit(W-sh) "Sash" mutant mice display aberrant myelopoiesis leading to the accumulation of splenocytes that act as myeloid-derived suppressor cells. J Immunol. 2013;190:5534-44 pubmed publisher
Seillet C, Rouquié N, Foulon E, Douin Echinard V, Krust A, Chambon P, et al. Estradiol promotes functional responses in inflammatory and steady-state dendritic cells through differential requirement for activation function-1 of estrogen receptor ?. J Immunol. 2013;190:5459-70 pubmed publisher
Saito Y, Kaneda K, Suekane A, Ichihara E, Nakahata S, Yamakawa N, et al. Maintenance of the hematopoietic stem cell pool in bone marrow niches by EVI1-regulated GPR56. Leukemia. 2013;27:1637-49 pubmed publisher
Jeannet R, Cai Q, Liu H, Vu H, Kuo Y. Alcam regulates long-term hematopoietic stem cell engraftment and self-renewal. Stem Cells. 2013;31:560-71 pubmed publisher
Althof N, Whitton J. Coxsackievirus B3 infects the bone marrow and diminishes the restorative capacity of erythroid and lymphoid progenitors. J Virol. 2013;87:2823-34 pubmed publisher
Xu H, Menendez S, Schlegelberger B, Bae N, Aplan P, Göhring G, et al. Loss of p53 accelerates the complications of myelodysplastic syndrome in a NUP98-HOXD13-driven mouse model. Blood. 2012;120:3089-97 pubmed publisher
Garaycoechea J, Crossan G, Langevin F, Daly M, Arends M, Patel K. Genotoxic consequences of endogenous aldehydes on mouse haematopoietic stem cell function. Nature. 2012;489:571-5 pubmed publisher
Ermakova O, Salimova E, Piszczek L, Gross C. Construction and phenotypic analysis of mice carrying a duplication of the major histocompatibility class I (MHC-I) locus. Mamm Genome. 2012;23:443-53 pubmed publisher
Mayle A, Luo M, Jeong M, Goodell M. Flow cytometry analysis of murine hematopoietic stem cells. Cytometry A. 2013;83:27-37 pubmed publisher
Gasparetto M, Sekulovic S, Zakaryan A, Imren S, Kent D, Humphries R, et al. Varying levels of aldehyde dehydrogenase activity in adult murine marrow hematopoietic stem cells are associated with engraftment and cell cycle status. Exp Hematol. 2012;40:857-66.e5 pubmed publisher
Sudo T, Yokota T, Oritani K, Satoh Y, Sugiyama T, Ishida T, et al. The endothelial antigen ESAM monitors hematopoietic stem cell status between quiescence and self-renewal. J Immunol. 2012;189:200-10 pubmed publisher
Florian M, Dörr K, Niebel A, Daria D, Schrezenmeier H, Rojewski M, et al. Cdc42 activity regulates hematopoietic stem cell aging and rejuvenation. Cell Stem Cell. 2012;10:520-30 pubmed publisher
Hinton R, O Neill H. Technical advance: in vitro production of distinct dendritic-like antigen-presenting cells from self-renewing hematopoietic stem cells. J Leukoc Biol. 2012;91:341-6 pubmed publisher
Brickshawana A, Shapiro V, Kita H, Pease L. Lineage(-)Sca1+c-Kit(-)CD25+ cells are IL-33-responsive type 2 innate cells in the mouse bone marrow. J Immunol. 2011;187:5795-804 pubmed publisher
Li L, Bailey E, Greenblatt S, Huso D, Small D. Loss of the wild-type allele contributes to myeloid expansion and disease aggressiveness in FLT3/ITD knockin mice. Blood. 2011;118:4935-45 pubmed publisher
Glotzbach J, Januszyk M, Vial I, Wong V, Gelbard A, Kalisky T, et al. An information theoretic, microfluidic-based single cell analysis permits identification of subpopulations among putatively homogeneous stem cells. PLoS ONE. 2011;6:e21211 pubmed publisher
Luche H, Ardouin L, Teo P, See P, Henri S, Merad M, et al. The earliest intrathymic precursors of CD8?(+) thymic dendritic cells correspond to myeloid-type double-negative 1c cells. Eur J Immunol. 2011;41:2165-75 pubmed publisher
Esplin B, Shimazu T, Welner R, Garrett K, Nie L, Zhang Q, et al. Chronic exposure to a TLR ligand injures hematopoietic stem cells. J Immunol. 2011;186:5367-75 pubmed publisher
Weishaupt H, Attema J. A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP-Chip. Biol Proced Online. 2010;12:1-17 pubmed publisher
Kaplan I, Morisot S, Heiser D, Cheng W, Kim M, Civin C. Deletion of tristetraprolin caused spontaneous reactive granulopoiesis by a non-cell-autonomous mechanism without disturbing long-term hematopoietic stem cell quiescence. J Immunol. 2011;186:2826-34 pubmed publisher
Åhsberg J, Tsapogas P, Qian H, Zetterblad J, Zandi S, Mansson R, et al. Interleukin-7-induced Stat-5 acts in synergy with Flt-3 signaling to stimulate expansion of hematopoietic progenitor cells. J Biol Chem. 2010;285:36275-84 pubmed publisher
Ji H, Ehrlich L, Seita J, Murakami P, Doi A, Lindau P, et al. Comprehensive methylome map of lineage commitment from haematopoietic progenitors. Nature. 2010;467:338-42 pubmed publisher
Böiers C, Buza Vidas N, Jensen C, Pronk C, Kharazi S, Wittmann L, et al. Expression and role of FLT3 in regulation of the earliest stage of normal granulocyte-monocyte progenitor development. Blood. 2010;115:5061-8 pubmed publisher
Fewkes N, Krauss A, Guimond M, Meadors J, Dobre S, Mackall C. Pharmacologic modulation of niche accessibility via tyrosine kinase inhibition enhances marrow and thymic engraftment after hematopoietic stem cell transplantation. Blood. 2010;115:4120-9 pubmed publisher
Chappaz S, Finke D. The IL-7 signaling pathway regulates lymph node development independent of peripheral lymphocytes. J Immunol. 2010;184:3562-9 pubmed publisher
Tan J, Periasamy P, O Neill H. Delineation of precursors in murine spleen that develop in contact with splenic endothelium to give novel dendritic-like cells. Blood. 2010;115:3678-85 pubmed publisher
Welner R, Esplin B, Garrett K, Pelayo R, Luche H, Fehling H, et al. Asynchronous RAG-1 expression during B lymphopoiesis. J Immunol. 2009;183:7768-77 pubmed publisher
Zlotoff D, Sambandam A, Logan T, Bell J, Schwarz B, Bhandoola A. CCR7 and CCR9 together recruit hematopoietic progenitors to the adult thymus. Blood. 2010;115:1897-905 pubmed publisher
Onai N, Manz M, Schmid M. Isolation of common dendritic cell progenitors (CDP) from mouse bone marrow. Methods Mol Biol. 2010;595:195-203 pubmed publisher
Papathanasiou P, Attema J, Karsunky H, Hosen N, Sontani Y, Hoyne G, et al. Self-renewal of the long-term reconstituting subset of hematopoietic stem cells is regulated by Ikaros. Stem Cells. 2009;27:3082-92 pubmed publisher
Serbina N, Hohl T, Cherny M, Pamer E. Selective expansion of the monocytic lineage directed by bacterial infection. J Immunol. 2009;183:1900-10 pubmed publisher
Papathanasiou P, Attema J, Karsunky H, Xu J, Smale S, Weissman I. Evaluation of the long-term reconstituting subset of hematopoietic stem cells with CD150. Stem Cells. 2009;27:2498-508 pubmed publisher
Cridland S, Keys J, Papathanasiou P, Perkins A. Indian hedgehog supports definitive erythropoiesis. Blood Cells Mol Dis. 2009;43:149-55 pubmed publisher
Maillard I, Chen Y, Friedman A, Yang Y, Tubbs A, Shestova O, et al. Menin regulates the function of hematopoietic stem cells and lymphoid progenitors. Blood. 2009;113:1661-9 pubmed publisher
Babbe H, McMenamin J, Hobeika E, Wang J, Rodig S, Reth M, et al. Genomic instability resulting from Blm deficiency compromises development, maintenance, and function of the B cell lineage. J Immunol. 2009;182:347-60 pubmed
Levi B, Yilmaz O, Duester G, Morrison S. Aldehyde dehydrogenase 1a1 is dispensable for stem cell function in the mouse hematopoietic and nervous systems. Blood. 2009;113:1670-80 pubmed publisher
Waskow C, Liu K, Darrasse Jèze G, Guermonprez P, Ginhoux F, Merad M, et al. The receptor tyrosine kinase Flt3 is required for dendritic cell development in peripheral lymphoid tissues. Nat Immunol. 2008;9:676-83 pubmed publisher
Singh P, Yao Y, Weliver A, Broxmeyer H, Hong S, Chang C. Vaccinia virus infection modulates the hematopoietic cell compartments in the bone marrow. Stem Cells. 2008;26:1009-16 pubmed publisher
Kohara H, Omatsu Y, Sugiyama T, Noda M, Fujii N, Nagasawa T. Development of plasmacytoid dendritic cells in bone marrow stromal cell niches requires CXCL12-CXCR4 chemokine signaling. Blood. 2007;110:4153-60 pubmed
Krueger A, Garbe A, von Boehmer H. Phenotypic plasticity of T cell progenitors upon exposure to Notch ligands. J Exp Med. 2006;203:1977-84 pubmed
Ceredig R, Rauch M, Balciunaite G, Rolink A. Increasing Flt3L availability alters composition of a novel bone marrow lymphoid progenitor compartment. Blood. 2006;108:1216-22 pubmed
Tussiwand R, Onai N, Mazzucchelli L, Manz M. Inhibition of natural type I IFN-producing and dendritic cell development by a small molecule receptor tyrosine kinase inhibitor with Flt3 affinity. J Immunol. 2005;175:3674-80 pubmed
Lawrence H, Christensen J, Fong S, Hu Y, Weissman I, Sauvageau G, et al. Loss of expression of the Hoxa-9 homeobox gene impairs the proliferation and repopulating ability of hematopoietic stem cells. Blood. 2005;106:3988-94 pubmed
Harman B, Jenkinson W, Parnell S, Rossi S, Jenkinson E, Anderson G. T/B lineage choice occurs prior to intrathymic Notch signaling. Blood. 2005;106:886-92 pubmed
Vosshenrich C, Ranson T, Samson S, Corcuff E, Colucci F, Rosmaraki E, et al. Roles for common cytokine receptor gamma-chain-dependent cytokines in the generation, differentiation, and maturation of NK cell precursors and peripheral NK cells in vivo. J Immunol. 2005;174:1213-21 pubmed
Mancini S, Mantei N, Dumortier A, Suter U, MacDonald H, Radtke F. Jagged1-dependent Notch signaling is dispensable for hematopoietic stem cell self-renewal and differentiation. Blood. 2005;105:2340-2 pubmed
product information
Product Type :
Antibody
Product Name :
CD135 (Flt3) Monoclonal Antibody (A2F10), APC, eBioscience
Catalog # :
17-1351-80
Quantity :
25 µg
Price :
US 102
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 1 µg/test
Species :
Mouse
Clone :
A2F10
Isotype :
IgG2a, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1 µg/test
Aliases :
B230315G04; CD135; CD135 antigen; fetal liver kinase 2; Fetal liver kinase-2; FL cytokine receptor; FLK2; Flk-2; FLT3; Flt-3; fms related tyrosine kinase 3; FMS-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Ly72; OTTHUMP00000214829; Receptor-type tyrosine-protein kinase FLT3; RP11-153M24.3; stem cell tyrosine kinase 1; STK1; STK-1; tyrosine-protein kinase FLT3; tyrosine-protein kinase receptor flk-2; wmfl
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA