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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD45.1 Monoclonal Antibody (A20), APC, eBioscience™
catalog :
17-0453-81
quantity :
50 µg
price :
US 309.00
clonality :
monoclonal
host :
mouse
conjugate :
APC
clone name :
A20
reactivity :
mouse
application :
immunocytochemistry, flow cytometry
more info or order :
citations: 82
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:200; loading ...; fig 5
Ward L, Lee D, Sharma A, Wang A, Naouar I, Ma X, et al. Siponimod therapy implicates Th17 cells in a preclinical model of subpial cortical injury. JCI Insight. 2020;5: pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig s4g
Ma L, Dichwalkar T, Chang J, Cossette B, Garafola D, Zhang A, et al. Enhanced CAR-T cell activity against solid tumors by vaccine boosting through the chimeric receptor. Science. 2019;365:162-168 pubmed publisher
  • flow cytometry; mouse; fig 4
Kamimura D, Katsunuma K, Arima Y, Atsumi T, Jiang J, Bando H, et al. KDEL receptor 1 regulates T-cell homeostasis via PP1 that is a key phosphatase for ISR. Nat Commun. 2015;6:7474 pubmed publisher
  • flow cytometry; mouse
Jacque E, Schweighoffer E, Visekruna A, Papoutsopoulou S, Janzen J, Zillwood R, et al. IKK-induced NF-κB1 p105 proteolysis is critical for B cell antibody responses to T cell-dependent antigen. J Exp Med. 2014;211:2085-101 pubmed publisher
  • flow cytometry; mouse; fig 3
Gondek D, Lu L, Quezada S, Sakaguchi S, Noelle R. Cutting edge: contact-mediated suppression by CD4+CD25+ regulatory cells involves a granzyme B-dependent, perforin-independent mechanism. J Immunol. 2005;174:1783-6 pubmed
Stokes J, Hoffman E, Molina M, Kummet N, Simpson R, Zeng Y, et al. Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia. Oncoimmunology. 2020;9:1758011 pubmed publisher
Elsner R, Shlomchik M. IL-12 Blocks Tfh Cell Differentiation during Salmonella Infection, thereby Contributing to Germinal Center Suppression. Cell Rep. 2019;29:2796-2809.e5 pubmed publisher
Wolf Y, Bartok O, Patkar S, Eli G, Cohen S, Litchfield K, et al. UVB-Induced Tumor Heterogeneity Diminishes Immune Response in Melanoma. Cell. 2019;179:219-235.e21 pubmed publisher
Kesarwani M, Kincaid Z, Azam M. Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence. J Vis Exp. 2019;: pubmed publisher
Beneke V, Küster F, Neehus A, Hesse C, Lopez Rodriguez E, Haake K, et al. An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages. Sci Rep. 2018;8:16281 pubmed publisher
Hamanaka S, Umino A, Sato H, Hayama T, Yanagida A, Mizuno N, et al. Generation of Vascular Endothelial Cells and Hematopoietic Cells by Blastocyst Complementation. Stem Cell Reports. 2018;11:988-997 pubmed publisher
Saint Fleur Lominy S, Maus M, Vaeth M, Lange I, Zee I, Suh D, et al. STIM1 and STIM2 Mediate Cancer-Induced Inflammation in T Cell Acute Lymphoblastic Leukemia. Cell Rep. 2018;24:3045-3060.e5 pubmed publisher
Mucci A, Lopez Rodriguez E, Hetzel M, Liu S, Suzuki T, Happle C, et al. iPSC-Derived Macrophages Effectively Treat Pulmonary Alveolar Proteinosis in Csf2rb-Deficient Mice. Stem Cell Reports. 2018;11:696-710 pubmed publisher
Cho S, Lee H, Yu I, Choi Y, Huang H, Hashemifar S, et al. Differential cell-intrinsic regulations of germinal center B and T cells by miR-146a and miR-146b. Nat Commun. 2018;9:2757 pubmed publisher
Schmidt K, Zhang Q, Tasdogan A, Petzold A, Dahl A, Arneth B, et al. The H3K4 methyltransferase Setd1b is essential for hematopoietic stem and progenitor cell homeostasis in mice. elife. 2018;7: pubmed publisher
Mitchell K, Barreyro L, Todorova T, Taylor S, Antony Debré I, Narayanagari S, et al. IL1RAP potentiates multiple oncogenic signaling pathways in AML. J Exp Med. 2018;215:1709-1727 pubmed publisher
Gaya M, Barral P, Burbage M, Aggarwal S, Montaner B, Warren Navia A, et al. Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells. Cell. 2018;172:517-533.e20 pubmed publisher
McGarvey A, Rybtsov S, Souilhol C, Tamagno S, Rice R, Hills D, et al. A molecular roadmap of the AGM region reveals BMPER as a novel regulator of HSC maturation. J Exp Med. 2017;214:3731-3751 pubmed publisher
Minutti C, Drube S, Blair N, Schwartz C, McCrae J, McKenzie A, et al. Epidermal Growth Factor Receptor Expression Licenses Type-2 Helper T Cells to Function in a T Cell Receptor-Independent Fashion. Immunity. 2017;47:710-722.e6 pubmed publisher
Kim S, Kim H, Yim Y, Ha S, Atarashi K, Tan T, et al. Maternal gut bacteria promote neurodevelopmental abnormalities in mouse offspring. Nature. 2017;549:528-532 pubmed publisher
Miao T, Symonds A, Singh R, Symonds J, Ogbe A, Omodho B, et al. Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation. J Exp Med. 2017;214:1787-1808 pubmed publisher
Ambrosi T, Scialdone A, Graja A, Gohlke S, Jank A, Bocian C, et al. Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration. Cell Stem Cell. 2017;20:771-784.e6 pubmed publisher
St Clair J, Detanico T, Aviszus K, Kirchenbaum G, Christie M, Carpenter J, et al. Immunogenicity of Isogenic IgG in Aggregates and Immune Complexes. PLoS ONE. 2017;12:e0170556 pubmed publisher
Shirai C, White B, Tripathi M, Tapia R, Ley J, Ndonwi M, et al. Mutant U2AF1-expressing cells are sensitive to pharmacological modulation of the spliceosome. Nat Commun. 2017;8:14060 pubmed publisher
Damgaard R, Walker J, Marco Casanova P, Morgan N, Titheradge H, Elliott P, et al. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. Cell. 2016;166:1215-1230.e20 pubmed publisher
Mucci A, Kunkiel J, Suzuki T, Brennig S, Glage S, Kühnel M, et al. Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency. Stem Cell Reports. 2016;7:292-305 pubmed publisher
Inoue S, Li W, Tseng A, Beerman I, Elia A, Bendall S, et al. Mutant IDH1 Downregulates ATM and Alters DNA Repair and Sensitivity to DNA Damage Independent of TET2. Cancer Cell. 2016;30:337-348 pubmed publisher
Souilhol C, Lendínez J, Rybtsov S, Murphy F, Wilson H, Hills D, et al. Developing HSCs become Notch independent by the end of maturation in the AGM region. Blood. 2016;128:1567-77 pubmed publisher
Rapp M, Grassmann S, Chaloupka M, Layritz P, Krüger S, Ormanns S, et al. C-C chemokine receptor type-4 transduction of T cells enhances interaction with dendritic cells, tumor infiltration and therapeutic efficacy of adoptive T cell transfer. Oncoimmunology. 2016;5:e1105428 pubmed
Coomes S, Kannan Y, Pelly V, Entwistle L, Guidi R, Perez Lloret J, et al. CD4+ Th2 cells are directly regulated by IL-10 during allergic airway inflammation. Mucosal Immunol. 2017;10:150-161 pubmed publisher
Ghosh M, Shapiro L. In vitro Ag Cross-presentation and in vivo Ag Cross-presentation by Dendritic Cells in the Mouse. Bio Protoc. 2012;2:e305 pubmed
de Valle E, Grigoriadis G, O Reilly L, Willis S, Maxwell M, Corcoran L, et al. NF?B1 is essential to prevent the development of multiorgan autoimmunity by limiting IL-6 production in follicular B cells. J Exp Med. 2016;213:621-41 pubmed publisher
Souilhol C, Gonneau C, Lendínez J, Batsivari A, Rybtsov S, Wilson H, et al. Inductive interactions mediated by interplay of asymmetric signalling underlie development of adult haematopoietic stem cells. Nat Commun. 2016;7:10784 pubmed publisher
Wu L, Li N, Zhang M, Xue S, Cassady K, Lin Q, et al. MHC-mismatched mixed chimerism augments thymic regulatory T-cell production and prevents relapse of EAE in mice. Proc Natl Acad Sci U S A. 2015;112:15994-9 pubmed publisher
Terhorst D, Chelbi R, Wohn C, Malosse C, Tamoutounour S, Jorquera A, et al. Dynamics and Transcriptomics of Skin Dendritic Cells and Macrophages in an Imiquimod-Induced, Biphasic Mouse Model of Psoriasis. J Immunol. 2015;195:4953-61 pubmed publisher
Abidin B, Owusu Kwarteng E, Heinonen K. Frizzled-6 Regulates Hematopoietic Stem/Progenitor Cell Survival and Self-Renewal. J Immunol. 2015;195:2168-76 pubmed publisher
Coomes S, Pelly V, Kannan Y, Okoye I, Czieso S, Entwistle L, et al. IFNγ and IL-12 Restrict Th2 Responses during Helminth/Plasmodium Co-Infection and Promote IFNγ from Th2 Cells. PLoS Pathog. 2015;11:e1004994 pubmed publisher
Jacque E, Schweighoffer E, Tybulewicz V, Ley S. BAFF activation of the ERK5 MAP kinase pathway regulates B cell survival. J Exp Med. 2015;212:883-92 pubmed publisher
Xue J, Sharma V, Hsieh M, Chawla A, Murali R, Pandol S, et al. Alternatively activated macrophages promote pancreatic fibrosis in chronic pancreatitis. Nat Commun. 2015;6:7158 pubmed publisher
Rybtsov S, Batsivari A, Bilotkach K, Paruzina D, Senserrich J, Nerushev O, et al. Tracing the origin of the HSC hierarchy reveals an SCF-dependent, IL-3-independent CD43(-) embryonic precursor. Stem Cell Reports. 2014;3:489-501 pubmed publisher
Xia P, Wang S, Huang G, Zhu P, Li M, Ye B, et al. WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner. J Exp Med. 2014;211:2119-34 pubmed publisher
Rodríguez Díez E, Quereda V, Bellutti F, Prchal Murphy M, Partida D, Eguren M, et al. Cdk4 and Cdk6 cooperate in counteracting the INK4 family of inhibitors during murine leukemogenesis. Blood. 2014;124:2380-90 pubmed publisher
Liakhovitskaia A, Rybtsov S, Smith T, Batsivari A, Rybtsova N, Rode C, et al. Runx1 is required for progression of CD41+ embryonic precursors into HSCs but not prior to this. Development. 2014;141:3319-23 pubmed publisher
Chen X, Fosco D, Kline D, Meng L, Nishi S, Savage P, et al. PD-1 regulates extrathymic regulatory T-cell differentiation. Eur J Immunol. 2014;44:2603-16 pubmed publisher
Rubinstein M, Salem M, Doedens A, Moore C, Chiuzan C, Rivell G, et al. G-CSF/anti-G-CSF antibody complexes drive the potent recovery and expansion of CD11b+Gr-1+ myeloid cells without compromising CD8+ T cell immune responses. J Hematol Oncol. 2013;6:75 pubmed publisher
Iannello A, Thompson T, Ardolino M, Lowe S, Raulet D. p53-dependent chemokine production by senescent tumor cells supports NKG2D-dependent tumor elimination by natural killer cells. J Exp Med. 2013;210:2057-69 pubmed publisher
Tada S, Yasui T, Nakatsuji Y, Okuno T, Koda T, Mochizuki H, et al. BAFF controls neural cell survival through BAFF receptor. PLoS ONE. 2013;8:e70924 pubmed publisher
Gordon Keylock S, Sobiesiak M, Rybtsov S, Moore K, Medvinsky A. Mouse extraembryonic arterial vessels harbor precursors capable of maturing into definitive HSCs. Blood. 2013;122:2338-45 pubmed publisher
Yu Y, Wang D, Kaosaard K, Liu C, Fu J, Haarberg K, et al. c-Rel is an essential transcription factor for the development of acute graft-versus-host disease in mice. Eur J Immunol. 2013;43:2327-37 pubmed publisher
Grosselin J, Sii Felice K, Payen E, Chretien S, Tronik Le Roux D, Leboulch P. Arrayed lentiviral barcoding for quantification analysis of hematopoietic dynamics. Stem Cells. 2013;31:2162-71 pubmed publisher
Toubai T, Sun Y, Luker G, Liu J, Luker K, Tawara I, et al. Host-derived CD8+ dendritic cells are required for induction of optimal graft-versus-tumor responses after experimental allogeneic bone marrow transplantation. Blood. 2013;121:4231-41 pubmed publisher
Reis B, Rogoz A, Costa Pinto F, Taniuchi I, Mucida D. Mutual expression of the transcription factors Runx3 and ThPOK regulates intestinal CD4? T cell immunity. Nat Immunol. 2013;14:271-80 pubmed publisher
Ma D, Suthar M, Kasahara S, Gale M, Clark E. CD22 is required for protection against West Nile virus Infection. J Virol. 2013;87:3361-75 pubmed publisher
Kaji T, Ishige A, Hikida M, Taka J, Hijikata A, Kubo M, et al. Distinct cellular pathways select germline-encoded and somatically mutated antibodies into immunological memory. J Exp Med. 2012;209:2079-97 pubmed publisher
Tang F, Wu Q, Ikenoue T, Guan K, Liu Y, Zheng P. A critical role for Rictor in T lymphopoiesis. J Immunol. 2012;189:1850-7 pubmed publisher
Yamada T, Park C, Burns A, Nakada D, Lacorazza H. The cytosolic protein G0S2 maintains quiescence in hematopoietic stem cells. PLoS ONE. 2012;7:e38280 pubmed publisher
Hadeiba H, Lahl K, Edalati A, Oderup C, Habtezion A, Pachynski R, et al. Plasmacytoid dendritic cells transport peripheral antigens to the thymus to promote central tolerance. Immunity. 2012;36:438-50 pubmed publisher
Gu P, Gao J, D Souza C, Kowalczyk A, Chou K, Zhang L. Trogocytosis of CD80 and CD86 by induced regulatory T cells. Cell Mol Immunol. 2012;9:136-46 pubmed publisher
Langlet C, Tamoutounour S, Henri S, Luche H, Ardouin L, Gregoire C, et al. CD64 expression distinguishes monocyte-derived and conventional dendritic cells and reveals their distinct role during intramuscular immunization. J Immunol. 2012;188:1751-60 pubmed publisher
He J, Vu D, Hunt G, Chugh A, Bhatnagar A, Bolli R. Human cardiac stem cells isolated from atrial appendages stably express c-kit. PLoS ONE. 2011;6:e27719 pubmed publisher
Bungartz G, Land H, Scadden D, Emerson S. NF-Y is necessary for hematopoietic stem cell proliferation and survival. Blood. 2012;119:1380-9 pubmed publisher
Yu Y, Wang D, Liu C, Kaosaard K, Semple K, Anasetti C, et al. Prevention of GVHD while sparing GVL effect by targeting Th1 and Th17 transcription factor T-bet and ROR?t in mice. Blood. 2011;118:5011-20 pubmed publisher
Halpert M, Thomas K, King R, Justement L. TLT2 potentiates neutrophil antibacterial activity and chemotaxis in response to G protein-coupled receptor-mediated signaling. J Immunol. 2011;187:2346-55 pubmed publisher
Rybtsov S, Sobiesiak M, Taoudi S, Souilhol C, Senserrich J, Liakhovitskaia A, et al. Hierarchical organization and early hematopoietic specification of the developing HSC lineage in the AGM region. J Exp Med. 2011;208:1305-15 pubmed publisher
Welch J, Yuan W, Ley T. PML-RARA can increase hematopoietic self-renewal without causing a myeloproliferative disease in mice. J Clin Invest. 2011;121:1636-45 pubmed publisher
Welch J, Klco J, Varghese N, Nagarajan R, Ley T. Rara haploinsufficiency modestly influences the phenotype of acute promyelocytic leukemia in mice. Blood. 2011;117:2460-8 pubmed publisher
Flutter B, Edwards N, Fallah Arani F, Henderson S, Chai J, Sivakumaran S, et al. Nonhematopoietic antigen blocks memory programming of alloreactive CD8+ T cells and drives their eventual exhaustion in mouse models of bone marrow transplantation. J Clin Invest. 2010;120:3855-68 pubmed publisher
Yauch L, Prestwood T, May M, Morar M, Zellweger R, Peters B, et al. CD4+ T cells are not required for the induction of dengue virus-specific CD8+ T cell or antibody responses but contribute to protection after vaccination. J Immunol. 2010;185:5405-16 pubmed publisher
Barber A, Sentman C. Chimeric NKG2D T cells require both T cell- and host-derived cytokine secretion and perforin expression to increase tumor antigen presentation and systemic immunity. J Immunol. 2009;183:2365-72 pubmed publisher
Nemeth M, Mak K, Yang Y, Bodine D. beta-Catenin expression in the bone marrow microenvironment is required for long-term maintenance of primitive hematopoietic cells. Stem Cells. 2009;27:1109-19 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
CD45.1 Monoclonal Antibody (A20), APC, eBioscience™
Catalog # :
17-0453-81
Quantity :
50 µg
Price :
US 309.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.5 µg/test
Species :
Mouse
Clone :
A20
Isotype :
IgG2a, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test
Aliases :
B220; Cd45; CD45 antigen; CD45R; GP180; Lca; L-CA; Leukocyte common antigen; loc; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA