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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD184 (CXCR4) Monoclonal Antibody (12G5), Functional Grade, eBioscience
catalog :
16-9999-85
quantity :
500 µg
price :
US 478
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
12G5
reactivity :
human
application :
neutralization, flow cytometry
more info or order :
Invitrogen product webpage
citations: 55
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; fig 1a
Koenen H, Smeets R, Vink P, van Rijssen E, Boots A, Joosten I. Human CD25highFoxp3pos regulatory T cells differentiate into IL-17-producing cells. Blood. 2008;112:2340-52 pubmed publisher
Wagner A, Pehar M, Yan Z, Kulka M. Amanita muscaria extract potentiates production of proinflammatory cytokines by dsRNA-activated human microglia. Front Pharmacol. 2023;14:1102465 pubmed publisher
Demchenko A, Kondrateva E, Tabakov V, Efremova A, Salikhova D, Bukharova T, et al. Airway and Lung Organoids from Human-Induced Pluripotent Stem Cells Can Be Used to Assess CFTR Conductance. Int J Mol Sci. 2023;24: pubmed publisher
Klusa D, Lohaus F, Franken A, Baumbach M, Cojoc M, Dowling P, et al. Dynamics of CXCR4 positive circulating tumor cells in prostate cancer patients during radiotherapy. Int J Cancer. 2023;152:2639-2654 pubmed publisher
Cheng W, Liu F, Ren Z, Chen W, Chen Y, Liu T, et al. Parallel functional assessment of m6A sites in human endodermal differentiation with base editor screens. Nat Commun. 2022;13:478 pubmed publisher
Burkard T, Dreis C, Herrero San Juan M, Huhn M, Weigert A, Pfeilschifter J, et al. Enhanced CXCR4 Expression of Human CD8Low T Lymphocytes Is Driven by S1P4. Front Immunol. 2021;12:668884 pubmed publisher
Nian Z, Zheng X, Dou Y, Du X, Zhou L, Fu B, et al. Rapamycin Pretreatment Rescues the Bone Marrow AML Cell Elimination Capacity of CAR-T Cells. Clin Cancer Res. 2021;27:6026-6038 pubmed publisher
Genkel V, Dolgushin I, Baturina I, Savochkina A, Kuznetsova A, Pykhova L, et al. Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia. Int J Inflam. 2021;2021:6695468 pubmed publisher
Di Paolo D, Pontis F, Moro M, Centonze G, Bertolini G, Milione M, et al. Cotargeting of miR-126-3p and miR-221-3p inhibits PIK3R2 and PTEN, reducing lung cancer growth and metastasis by blocking AKT and CXCR4 signalling. Mol Oncol. 2021;15:2969-2988 pubmed publisher
Zhang S, Jiang V, Han G, Hao D, Lian J, Liu Y, et al. Longitudinal single-cell profiling reveals molecular heterogeneity and tumor-immune evolution in refractory mantle cell lymphoma. Nat Commun. 2021;12:2877 pubmed publisher
Choi Y, Kim Y, Oh S, Suh K, Kim Y, Lee G, et al. Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer. Adv Sci (Weinh). 2021;8:2002497 pubmed publisher
Mehta A, Tahhan A, Liu C, Dhindsa D, Nayak A, Hooda A, et al. Circulating Progenitor Cells in Patients With Coronary Artery Disease and Renal Insufficiency. JACC Basic Transl Sci. 2020;5:770-782 pubmed publisher
OhAinle M, Helms L, Vermeire J, Roesch F, Humes D, Basom R, et al. A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV. elife. 2018;7: pubmed publisher
Berning P, Schaefer C, Clemens D, Korsching E, Dirksen U, Potratz J. The CXCR4 antagonist plerixafor (AMD3100) promotes proliferation of Ewing sarcoma cell lines in vitro and activates receptor tyrosine kinase signaling. Cell Commun Signal. 2018;16:21 pubmed publisher
Zheng J, Wang H, Zhou W. Modulatory effects of trophoblast-secreted CXCL12 on the migration and invasion of human first-trimester decidual epithelial cells are mediated by CXCR4 rather than CXCR7. Reprod Biol Endocrinol. 2018;16:17 pubmed publisher
Webber B, Osborn M, McElroy A, Twaroski K, Lonetree C, Defeo A, et al. CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa. NPJ Regen Med. 2016;1: pubmed publisher
Berger R, Sun Y, Kulik M, Lee J, Nairn A, Moremen K, et al. ST8SIA4-Dependent Polysialylation is Part of a Developmental Program Required for Germ Layer Formation from Human Pluripotent Stem Cells. Stem Cells. 2016;34:1742-52 pubmed publisher
Han A, Lee J, Kim H, Min W, Park G, Kim S. A CXCR4 antagonist leads to tumor suppression by activation of immune cells in a leukemia-induced microenvironment. Oncol Rep. 2015;34:2880-8 pubmed publisher
Hayasaka H, Kobayashi D, Yoshimura H, Nakayama E, Shioda T, Miyasaka M. The HIV-1 Gp120/CXCR4 axis promotes CCR7 ligand-dependent CD4 T cell migration: CCR7 homo- and CCR7/CXCR4 hetero-oligomer formation as a possible mechanism for up-regulation of functional CCR7. PLoS ONE. 2015;10:e0117454 pubmed publisher
Tian L, Prasad N, Jang Y. In Vitro Modeling of Alcohol-Induced Liver Injury Using Human-Induced Pluripotent Stem Cells. Methods Mol Biol. 2016;1353:271-83 pubmed publisher
Pelekanos R, Ting M, Sardesai V, Ryan J, Lim Y, Chan J, et al. Intracellular trafficking and endocytosis of CXCR4 in fetal mesenchymal stem/stromal cells. BMC Cell Biol. 2014;15:15 pubmed publisher
Beider K, Darash Yahana M, Blaier O, Koren Michowitz M, Abraham M, Wald H, et al. Combination of imatinib with CXCR4 antagonist BKT140 overcomes the protective effect of stroma and targets CML in vitro and in vivo. Mol Cancer Ther. 2014;13:1155-69 pubmed publisher
Ray Saha S, Huber T, Sakmar T. Antibody epitopes on g protein-coupled receptors mapped with genetically encoded photoactivatable cross-linkers. Biochemistry. 2014;53:1302-10 pubmed publisher
Serrano Pertierra E, Cernuda Morollón E, Lopez Larrea C. NKG2D- and CD28-mediated costimulation regulate CD8+ T cell chemotaxis through different mechanisms: the role of Cdc42/N-WASp. J Leukoc Biol. 2014;95:487-95 pubmed publisher
Miles B, Zakhary I, El Awady A, Scisci E, CARRION J, O Neill J, et al. Secondary lymphoid organ homing phenotype of human myeloid dendritic cells disrupted by an intracellular oral pathogen. Infect Immun. 2014;82:101-11 pubmed publisher
Cimato T, Palka B, Lang J, Young R. LDL cholesterol modulates human CD34+ HSPCs through effects on proliferation and the IL-17 G-CSF axis. PLoS ONE. 2013;8:e73861 pubmed publisher
Park B, Kook S, Lee S, Jeong J, Brufsky A, Lee B. An isoform of C/EBP?, LIP, regulates expression of the chemokine receptor CXCR4 and modulates breast cancer cell migration. J Biol Chem. 2013;288:28656-67 pubmed publisher
Peters J, Koenen H, Fasse E, Tijssen H, Ijzermans J, Groenen P, et al. Human secondary lymphoid organs typically contain polyclonally-activated proliferating regulatory T cells. Blood. 2013;122:2213-23 pubmed publisher
Turaç G, Hindley C, Thomas R, Davis J, Deleidi M, Gasser T, et al. Combined flow cytometric analysis of surface and intracellular antigens reveals surface molecule markers of human neuropoiesis. PLoS ONE. 2013;8:e68519 pubmed publisher
Lu C, Guo J, Gu J, Ge D, Hou Y, Lin Z, et al. CXCR4 heterogeneous expression in esophageal squamous cell cancer and stronger metastatic potential with CXCR4-positive cancer cells. Dis Esophagus. 2014;27:294-302 pubmed publisher
Cany J, van der Waart A, Tordoir M, Franssen G, Hangalapura B, De Vries J, et al. Natural killer cells generated from cord blood hematopoietic progenitor cells efficiently target bone marrow-residing human leukemia cells in NOD/SCID/IL2Rg(null) mice. PLoS ONE. 2013;8:e64384 pubmed publisher
Sison E, McIntyre E, Magoon D, Brown P. Dynamic chemotherapy-induced upregulation of CXCR4 expression: a mechanism of therapeutic resistance in pediatric AML. Mol Cancer Res. 2013;11:1004-16 pubmed publisher
Lim S, Kim T, Lee J, Sonn C, Kim K, Kim J, et al. Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy. Cancer Res. 2013;73:2598-607 pubmed publisher
Rieber N, Brand A, Hector A, Graepler Mainka U, Ost M, Schäfer I, et al. Flagellin induces myeloid-derived suppressor cells: implications for Pseudomonas aeruginosa infection in cystic fibrosis lung disease. J Immunol. 2013;190:1276-84 pubmed publisher
Warner J, Zwezdaryk K, Day B, Sullivan D, Pridjian G, Morris C. Human cytomegalovirus infection inhibits CXCL12- mediated migration and invasion of human extravillous cytotrophoblasts. Virol J. 2012;9:255 pubmed publisher
Frick S, Bacher N, Baier G, Mailänder V, Landfester K, Steinbrink K. Functionalized polystyrene nanoparticles trigger human dendritic cell maturation resulting in enhanced CD4+ T cell activation. Macromol Biosci. 2012;12:1637-47 pubmed publisher
Danova Alt R, Heider A, Egger D, Cross M, Alt R. Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics. PLoS ONE. 2012;7:e34899 pubmed publisher
Kroenke M, Eto D, Locci M, Cho M, Davidson T, Haddad E, et al. Bcl6 and Maf cooperate to instruct human follicular helper CD4 T cell differentiation. J Immunol. 2012;188:3734-44 pubmed publisher
Prabakar K, Dominguez Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, et al. Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells. Cell Transplant. 2012;21:1321-39 pubmed publisher
Kean L, Sen S, Onabajo O, Singh K, Robertson J, Stempora L, et al. Significant mobilization of both conventional and regulatory T cells with AMD3100. Blood. 2011;118:6580-90 pubmed publisher
Hildebrandt M, Dijkstra D, Gollasch H, Daemen K, Stevanovic Meyer M, Ludwig W. Apheresis-related enrichment of CD26++ T lymphocytes: phenotypic characterization and correlation with unfavorable outcome in autologous hematopoietic progenitor cell transplantation. Transfusion. 2012;52:765-76 pubmed publisher
Zhang R, Pan X, Huang Z, Weber G, Zhang G. Osteopontin enhances the expression and activity of MMP-2 via the SDF-1/CXCR4 axis in hepatocellular carcinoma cell lines. PLoS ONE. 2011;6:e23831 pubmed publisher
Cheng M, Ma J, Chen Y, Zhang J, Zhao W, Zhang J, et al. Establishment, characterization, and successful adaptive therapy against human tumors of NKG cell, a new human NK cell line. Cell Transplant. 2011;20:1731-46 pubmed publisher
Rasmussen T, Andersen T, Hvid M, Hetland M, Hørslev Petersen K, Stengaard Pedersen K, et al. Increased interleukin 21 (IL-21) and IL-23 are associated with increased disease activity and with radiographic status in patients with early rheumatoid arthritis. J Rheumatol. 2010;37:2014-20 pubmed publisher
Kwajah M M S, Schwarz H. CD137 ligand signaling induces human monocyte to dendritic cell differentiation. Eur J Immunol. 2010;40:1938-49 pubmed publisher
Ali Z, Yan L, Plagman N, Reichenberg A, Hintz M, Jomaa H, et al. Gammadelta T cell immune manipulation during chronic phase of simian-human immunodeficiency virus infection [corrected] confers immunological benefits. J Immunol. 2009;183:5407-17 pubmed publisher
Huang E, Singh B, Cristofanilli M, Gelovani J, Wei C, Vincent L, et al. A CXCR4 antagonist CTCE-9908 inhibits primary tumor growth and metastasis of breast cancer. J Surg Res. 2009;155:231-6 pubmed publisher
Dubeykovskaya Z, Dubeykovskiy A, Solal Cohen J, Wang T. Secreted trefoil factor 2 activates the CXCR4 receptor in epithelial and lymphocytic cancer cell lines. J Biol Chem. 2009;284:3650-62 pubmed publisher
Mikami S, Nakase H, Yamamoto S, Takeda Y, Yoshino T, Kasahara K, et al. Blockade of CXCL12/CXCR4 axis ameliorates murine experimental colitis. J Pharmacol Exp Ther. 2008;327:383-92 pubmed publisher
Li G, Basu S, Han M, Kim Y, Broxmeyer H. Influence of ERK activation on decreased chemotaxis of mature human cord blood monocyte-derived dendritic cells to CCL19 and CXCL12. Blood. 2007;109:3173-6 pubmed
Zhang C, Cui G, Liu F, Wu Q, Chen Y. Inhibitory effect of triptolide on lymph node metastasis in patients with non-Hodgkin lymphoma by regulating SDF-1/CXCR4 axis in vitro. Acta Pharmacol Sin. 2006;27:1438-46 pubmed
Laudanski K, De A, Pellegrini J, Miller Graziano C. Simultaneous aberrations in Mphi and T cell function adversely affect trauma patients' clinical outcome: a possible faulty IL-13 feedback loop. Clin Immunol. 2006;118:332-41 pubmed
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Pelus L, Bian H, Fukuda S, Wong D, Merzouk A, Salari H. The CXCR4 agonist peptide, CTCE-0021, rapidly mobilizes polymorphonuclear neutrophils and hematopoietic progenitor cells into peripheral blood and synergizes with granulocyte colony-stimulating factor. Exp Hematol. 2005;33:295-307 pubmed
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product information
Product Type :
Antibody
Product Name :
CD184 (CXCR4) Monoclonal Antibody (12G5), Functional Grade, eBioscience
Catalog # :
16-9999-85
Quantity :
500 µg
Price :
US 478
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 0.5 µg/test, Functional Assay: Assay-Dependent, Neutralization: Assay-Dependent
Species :
Human
Clone :
12G5
Isotype :
IgG2a, kappa
Storage :
4° C
Description :
CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test, Functional Assay: Assay-Dependent, Neutralization: Assay-Dependent
Aliases :
b2b220Clo; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; CD184 antigen; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C) receptor 4; chemokine receptor; chemokine receptor (LCR1); chemokine receptor 4; chemokine receptor CXCR4; Cmkar4; CXC; CXC chemokine receptor; C-X-C chemokine receptor type 4; CXC motif chemokine; C-X-C motif chemokine receptor 4; CXCR4; CXC-R4; CXCR-4; D2S201E; FB22; Fusin; HM89; HSY3RR; HUMSTR; LAP3; LAP-3; LCR1; LESTR; Leukocyte-derived seven transmembrane domain receptor; leukocyte-expressed seven-transmembrane-domain; lipopolysaccharide-associated protein 3; LPS-associated protein 3; neuropeptide Y receptor Y3; neuropeptide Y3 receptor; NPY3R; NPYR; NPYRL; NPYY3R; PB-CKR; PBSF/SDF-1; pre-B-cell-derived chemokine receptor; SDF-1 receptor; Sdf1r; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; stromal cell-derived factor 1 receptor; WHIM; WHIMS
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA