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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD279 (PD-1) Monoclonal Antibody (J43), Functional Grade, eBioscience
catalog :
16-9985-85
quantity :
500 µg
price :
US 429
clonality :
monoclonal
host :
hamsters
conjugate :
nonconjugated
clone name :
J43
reactivity :
human, mouse
application :
flow cytometry, blocking or activating experiments
more info or order :
citations: 74
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:200; loading ...; fig 3e
Stebegg M, Bignon A, Hill D, Silva Cayetano A, Krueger C, Vanderleyden I, et al. Rejuvenating conventional dendritic cells and T follicular helper cell formation after vaccination. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Pan X, Ma B, You X, Chen S, Wu J, Wang T, et al. Synthesized natural peptides from amphibian skin secretions increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site. BMC Complement Altern Med. 2019;19:163 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Eldi P, Chaudhri G, Nutt S, Newsome T, Karupiah G. Viral Replicative Capacity, Antigen Availability via Hematogenous Spread, and High TFH:TFR Ratios Drive Induction of Potent Neutralizing Antibody Responses. J Virol. 2019;93: pubmed publisher
  • flow cytometry; mouse; fig 5
Seifert A, Zeng S, Zhang J, Kim T, Cohen N, Beckman M, et al. PD-1/PD-L1 Blockade Enhances T-cell Activity and Antitumor Efficacy of Imatinib in Gastrointestinal Stromal Tumors. Clin Cancer Res. 2017;23:454-465 pubmed publisher
  • flow cytometry; mouse; 1:100; fig 4, 7
McDonald P, Read K, Baker C, Anderson A, Powell M, Ballesteros Tato A, et al. IL-7 signalling represses Bcl-6 and the TFH gene program. Nat Commun. 2016;7:10285 pubmed publisher
  • flow cytometry; mouse; fig 4
Black L, Srivastava R, Schoeb T, Moore R, Barnes S, KABAROWSKI J. Cholesterol-Independent Suppression of Lymphocyte Activation, Autoimmunity, and Glomerulonephritis by Apolipoprotein A-I in Normocholesterolemic Lupus-Prone Mice. J Immunol. 2015;195:4685-98 pubmed publisher
  • flow cytometry; mouse
Xin L, Jiang T, Kinder J, Ertelt J, Way S. Infection susceptibility and immune senescence with advancing age replicated in accelerated aging Lmna(Dhe) mice. Aging Cell. 2015;14:1122-6 pubmed publisher
  • flow cytometry; mouse; fig 2
Choi Y, Gullicksrud J, Xing S, Zeng Z, Shan Q, Li F, et al. LEF-1 and TCF-1 orchestrate T(FH) differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6. Nat Immunol. 2015;16:980-90 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1e
Ngiow S, Young A, Jacquelot N, Yamazaki T, Enot D, Zitvogel L, et al. A Threshold Level of Intratumor CD8+ T-cell PD1 Expression Dictates Therapeutic Response to Anti-PD1. Cancer Res. 2015;75:3800-11 pubmed publisher
  • flow cytometry; mouse; fig s2
Kim Y, Lim H, Jung H, Wetsel R, Chung Y. Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells. PLoS ONE. 2015;10:e0120294 pubmed publisher
  • flow cytometry; mouse; fig 6
Clouthier D, Zhou A, Wortzman M, Luft O, Levy G, Watts T. GITR intrinsically sustains early type 1 and late follicular helper CD4 T cell accumulation to control a chronic viral infection. PLoS Pathog. 2015;11:e1004517 pubmed publisher
  • flow cytometry; mouse; fig 3
Shrestha S, Yang K, Guy C, Vogel P, Neale G, Chi H. Treg cells require the phosphatase PTEN to restrain TH1 and TFH cell responses. Nat Immunol. 2015;16:178-87 pubmed publisher
  • flow cytometry; mouse
Guo X, Tanaka Y, Kondo M. Thymic precursors of TCRαβ(+)CD8αα(+) intraepithelial lymphocytes are negative for CD103. Immunol Lett. 2015;163:40-8 pubmed publisher
  • flow cytometry; mouse; fig 2
Buchan S, Manzo T, Flutter B, Rogel A, Edwards N, Zhang L, et al. OX40- and CD27-mediated costimulation synergizes with anti-PD-L1 blockade by forcing exhausted CD8+ T cells to exit quiescence. J Immunol. 2015;194:125-133 pubmed publisher
  • flow cytometry; human
Zhu Y, Knolhoff B, Meyer M, Nywening T, West B, Luo J, et al. CSF1/CSF1R blockade reprograms tumor-infiltrating macrophages and improves response to T-cell checkpoint immunotherapy in pancreatic cancer models. Cancer Res. 2014;74:5057-69 pubmed publisher
  • flow cytometry; mouse
Vogelzang A, Perdomo C, Zedler U, Kuhlmann S, Hurwitz R, Gengenbacher M, et al. Central memory CD4+ T cells are responsible for the recombinant Bacillus Calmette-Guérin ?ureC::hly vaccine's superior protection against tuberculosis. J Infect Dis. 2014;210:1928-37 pubmed publisher
  • flow cytometry; mouse; fig 3
Van Der Werf N, Redpath S, Azuma M, Yagita H, Taylor M. Th2 cell-intrinsic hypo-responsiveness determines susceptibility to helminth infection. PLoS Pathog. 2013;9:e1003215 pubmed publisher
  • flow cytometry; mouse
Wollenberg I, Agua Doce A, Hernandez A, Almeida C, Oliveira V, Faro J, et al. Regulation of the germinal center reaction by Foxp3+ follicular regulatory T cells. J Immunol. 2011;187:4553-60 pubmed publisher
  • flow cytometry; mouse; fig 1
Tousif S, Singh Y, Prasad D, Sharma P, Van Kaer L, Das G. T cells from Programmed Death-1 deficient mice respond poorly to Mycobacterium tuberculosis infection. PLoS ONE. 2011;6:e19864 pubmed publisher
  • flow cytometry; mouse; fig 1
Nakae S, Iwakura Y, Suto H, Galli S. Phenotypic differences between Th1 and Th17 cells and negative regulation of Th1 cell differentiation by IL-17. J Leukoc Biol. 2007;81:1258-68 pubmed
  • blocking or activating experiments; mouse
Kuipers H, Muskens F, Willart M, Hijdra D, van Assema F, Coyle A, et al. Contribution of the PD-1 ligands/PD-1 signaling pathway to dendritic cell-mediated CD4+ T cell activation. Eur J Immunol. 2006;36:2472-82 pubmed
  • blocking or activating experiments; mouse; 10 ug/ml
  • flow cytometry; mouse
Schoop R, Wahl P, Le Hir M, Heemann U, Wang M, Wuthrich R. Suppressed T-cell activation by IFN-gamma-induced expression of PD-L1 on renal tubular epithelial cells. Nephrol Dial Transplant. 2004;19:2713-20 pubmed
Li Q, Zhang L, You W, Xu J, Dai J, Hua D, et al. PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells. Nat Commun. 2022;13:7677 pubmed publisher
Shen S, Li Y, Jin M, Fan D, Pan R, Lin A, et al. CD4+ CTLs Act as a Key Effector Population for Allograft Rejection of MSCs in a Donor MHC-II Dependent Manner in Injured Liver. Aging Dis. 2022;13:1919-1938 pubmed publisher
Wang W, Wu S, Cen Z, Zhang Y, Chen Y, Huang Y, et al. Mobilizing phospholipids on tumor plasma membrane implicates phosphatidylserine externalization blockade for cancer immunotherapy. Cell Rep. 2022;41:111582 pubmed publisher
Hu J, He J, Wang Y, Zhao Y, Fang K, Dong Y, et al. Ultrasound combined with nanobubbles promotes systemic anticancer immunity and augments anti-PD1 efficacy. J Immunother Cancer. 2022;10: pubmed publisher
Sato Y, Oguchi A, Fukushima Y, Masuda K, Toriu N, Taniguchi K, et al. CD153/CD30 signaling promotes age-dependent tertiary lymphoid tissue expansion and kidney injury. J Clin Invest. 2022;132: pubmed publisher
Wu G, Murugesan G, Nagala M, McCraw A, Haslam S, Dell A, et al. Activation of regulatory T cells triggers specific changes in glycosylation associated with Siglec-1-dependent inflammatory responses. Wellcome Open Res. 2021;6:134 pubmed publisher
Wilfahrt D, Philips R, Lama J, Kizerwetter M, Shapiro M, McCue S, et al. Histone deacetylase 3 represses cholesterol efflux during CD4+ T-cell activation. elife. 2021;10: pubmed publisher
Kalim K, Yang J, Modur V, Nguyen P, Li Y, Zheng Y, et al. Graded RhoA GTPase Expression in Treg Cells Distinguishes Tumor Immunity From Autoimmunity. Front Immunol. 2021;12:726393 pubmed publisher
Sun Y, Lu D, Yin Y, Song J, Liu Y, Hao W, et al. PTENα functions as an immune suppressor and promotes immune resistance in PTEN-mutant cancer. Nat Commun. 2021;12:5147 pubmed publisher
Zhou X, Zhu X, Li C, Li Y, Ye Z, Shapiro V, et al. Stearoyl-CoA Desaturase-Mediated Monounsaturated Fatty Acid Availability Supports Humoral Immunity. Cell Rep. 2021;34:108601 pubmed publisher
Gu M, Zhou X, Sohn J, Zhu L, Jie Z, Yang J, et al. NF-κB-inducing kinase maintains T cell metabolic fitness in antitumor immunity. Nat Immunol. 2021;22:193-204 pubmed publisher
Ferrere G, Tidjani Alou M, Liu P, Goubet A, Fidelle M, Kepp O, et al. Ketogenic diet and ketone bodies enhance the anticancer effects of PD-1 blockade. JCI Insight. 2021;6: pubmed publisher
Nguyen H, Ticer T, Bastian D, Kuril S, Li H, Du H, et al. Lysosomal Acid Lipase Is Required for Donor T Cells to Induce Graft-versus-Host Disease. Cell Rep. 2020;33:108316 pubmed publisher
Lee H, Park J, Yoo H, Lee H, Lee B, Kim J. The Selenoprotein MsrB1 Instructs Dendritic Cells to Induce T-Helper 1 Immune Responses. Antioxidants (Basel). 2020;9: pubmed publisher
Ye X, Waite J, Dhanik A, Gupta N, Zhong M, Adler C, et al. Endogenous retroviral proteins provide an immunodominant but not requisite antigen in a murine immunotherapy tumor model. Oncoimmunology. 2020;9:1758602 pubmed publisher
Fumagalli V, Di Lucia P, Venzin V, Bono E, Jordan R, Frey C, et al. Serum HBsAg clearance has minimal impact on CD8+ T cell responses in mouse models of HBV infection. J Exp Med. 2020;217: pubmed publisher
Shang K, Wang Z, Hu Y, Huang Y, Yuan K, Yu Y. Gene silencing of indoleamine 2,3-dioxygenase 1 inhibits lung cancer growth by suppressing T-cell exhaustion. Oncol Lett. 2020;19:3827-3838 pubmed publisher
Doo D, Meza Perez S, Londoño A, Goldsberry W, Katre A, Boone J, et al. Inhibition of the Wnt/β-catenin pathway enhances antitumor immunity in ovarian cancer. Ther Adv Med Oncol. 2020;12:1758835920913798 pubmed publisher
Ebelt N, Zuniga E, Johnson B, Diamond D, Manuel E. 5-Azacytidine Potentiates Anti-tumor Immunity in a Model of Pancreatic Ductal Adenocarcinoma. Front Immunol. 2020;11:538 pubmed publisher
Arroyo E, Pepper M. B cells are sufficient to prime the dominant CD4+ Tfh response to Plasmodium infection. J Exp Med. 2020;217: pubmed publisher
Burrack A, Spartz E, Raynor J, Wang I, Olson M, Stromnes I. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019;28:2140-2155.e6 pubmed publisher
Zhang X, Shi X, Li J, Mo L, Hu Z, Gao J, et al. PD-1 Blockade Overcomes Adaptive Immune Resistance in Treatment with Anchored-GM-CSF Bladder Cancer Cells Vaccine. J Cancer. 2018;9:4374-4381 pubmed publisher
Gaya M, Barral P, Burbage M, Aggarwal S, Montaner B, Warren Navia A, et al. Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells. Cell. 2018;172:517-533.e20 pubmed publisher
Deng R, Hurtz C, Song Q, Yue C, Xiao G, Yu H, et al. Extrafollicular CD4+ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease. Nat Commun. 2017;8:978 pubmed publisher
Yang K, Blanco D, Neale G, Vogel P, Avila J, Clish C, et al. Homeostatic control of metabolic and functional fitness of Treg cells by LKB1 signalling. Nature. 2017;548:602-606 pubmed publisher
Peer S, Baier G, Gruber T. Cblb-deficient T cells are less susceptible to PD-L1-mediated inhibition. Oncotarget. 2017;8:41841-41853 pubmed publisher
Jiang H, Rivera Molina Y, Gomez Manzano C, Clise Dwyer K, Bover L, Vence L, et al. Oncolytic Adenovirus and Tumor-Targeting Immune Modulatory Therapy Improve Autologous Cancer Vaccination. Cancer Res. 2017;77:3894-3907 pubmed publisher
Liu Y, Marin A, Ejlerskov P, Rasmussen L, Prinz M, Issazadeh Navikas S. Neuronal IFN-beta-induced PI3K/Akt-FoxA1 signalling is essential for generation of FoxA1+Treg cells. Nat Commun. 2017;8:14709 pubmed publisher
Fabian K, Chi Sabins N, Taylor J, Fecek R, Weinstein A, Storkus W. Therapeutic efficacy of combined vaccination against tumor pericyte-associated antigens DLK1 and DLK2 in mice. Oncoimmunology. 2017;6:e1290035 pubmed publisher
Zamarin D, Holmgaard R, Ricca J, Plitt T, Palese P, Sharma P, et al. Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity. Nat Commun. 2017;8:14340 pubmed publisher
Sonner J, Deumelandt K, Ott M, Thomé C, Rauschenbach K, Schulz S, et al. The stress kinase GCN2 does not mediate suppression of antitumor T cell responses by tryptophan catabolism in experimental melanomas. Oncoimmunology. 2016;5:e1240858 pubmed publisher
Chen S, Cai C, Li Z, Liu G, Wang Y, Blonska M, et al. Dissection of SAP-dependent and SAP-independent SLAM family signaling in NKT cell development and humoral immunity. J Exp Med. 2017;214:475-489 pubmed publisher
Chen L, Yi X, Goswami S, Ahn Y, Roybal J, Yang Y, et al. Growth and metastasis of lung adenocarcinoma is potentiated by BMP4-mediated immunosuppression. Oncoimmunology. 2016;5:e1234570 pubmed publisher
Tian Y, Kuo C, Akbari O, Ou J. Maternal-Derived Hepatitis B Virus e Antigen Alters Macrophage Function in Offspring to Drive Viral Persistence after Vertical Transmission. Immunity. 2016;44:1204-14 pubmed publisher
Tähtinen S, Kaikkonen S, Merisalo Soikkeli M, Grönberg Vähä Koskela S, Kanerva A, Parviainen S, et al. Favorable alteration of tumor microenvironment by immunomodulatory cytokines for efficient T-cell therapy in solid tumors. PLoS ONE. 2015;10:e0131242 pubmed publisher
Li C, Li W, Xiao J, Jiao S, Teng F, Xue S, et al. ADAP and SKAP55 deficiency suppresses PD-1 expression in CD8+ cytotoxic T lymphocytes for enhanced anti-tumor immunotherapy. EMBO Mol Med. 2015;7:754-69 pubmed publisher
Miyazaki M, Miyazaki K, Chen S, Chandra V, Wagatsuma K, Agata Y, et al. The E-Id protein axis modulates the activities of the PI3K-AKT-mTORC1-Hif1a and c-myc/p19Arf pathways to suppress innate variant TFH cell development, thymocyte expansion, and lymphomagenesis. Genes Dev. 2015;29:409-25 pubmed publisher
Chen L, Gibbons D, Goswami S, Cortez M, Ahn Y, Byers L, et al. Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression. Nat Commun. 2014;5:5241 pubmed publisher
Schachtele S, Hu S, Sheng W, Mutnal M, Lokensgard J. Glial cells suppress postencephalitic CD8+ T lymphocytes through PD-L1. Glia. 2014;62:1582-94 pubmed publisher
Chung J, Tamura K, Akiyoshi H, Cruz P, Ariizumi K. The DC-HIL/syndecan-4 pathway regulates autoimmune responses through myeloid-derived suppressor cells. J Immunol. 2014;192:2576-84 pubmed publisher
Zumwalde N, Domae E, Mescher M, Shimizu Y. ICAM-1-dependent homotypic aggregates regulate CD8 T cell effector function and differentiation during T cell activation. J Immunol. 2013;191:3681-93 pubmed publisher
Chang K, Burnham C, Compton S, Rasche D, Mazuski R, McDonough J, et al. Blockade of the negative co-stimulatory molecules PD-1 and CTLA-4 improves survival in primary and secondary fungal sepsis. Crit Care. 2013;17:R85 pubmed publisher
Kearl T, Jing W, Gershan J, Johnson B. Programmed death receptor-1/programmed death receptor ligand-1 blockade after transient lymphodepletion to treat myeloma. J Immunol. 2013;190:5620-8 pubmed publisher
Langenhorst D, Gogishvili T, Ribechini E, Kneitz S, McPherson K, Lutz M, et al. Sequential induction of effector function, tissue migration and cell death during polyclonal activation of mouse regulatory T-cells. PLoS ONE. 2012;7:e50080 pubmed publisher
Khan O, Headley M, Gérard A, Wei W, Liu L, Krummel M. Regulation of T cell priming by lymphoid stroma. PLoS ONE. 2011;6:e26138 pubmed publisher
Yang W, Yamada M, Tamura Y, Chang K, Mao J, Zou L, et al. Farnesyltransferase inhibitor FTI-277 reduces mortality of septic mice along with improved bacterial clearance. J Pharmacol Exp Ther. 2011;339:832-41 pubmed publisher
Kashiwakuma D, Suto A, Hiramatsu Y, Ikeda K, Takatori H, Suzuki K, et al. B and T lymphocyte attenuator suppresses IL-21 production from follicular Th cells and subsequent humoral immune responses. J Immunol. 2010;185:2730-6 pubmed publisher
Wang C, McDonald K, McDonough J, Newberry R. Murine isolated lymphoid follicles contain follicular B lymphocytes with a mucosal phenotype. Am J Physiol Gastrointest Liver Physiol. 2006;291:G595-604 pubmed
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Salama A, Chitnis T, Imitola J, Ansari M, Akiba H, Tushima F, et al. Critical role of the programmed death-1 (PD-1) pathway in regulation of experimental autoimmune encephalomyelitis. J Exp Med. 2003;198:71-8 pubmed
Ansari M, Salama A, Chitnis T, Smith R, Yagita H, Akiba H, et al. The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice. J Exp Med. 2003;198:63-9 pubmed
Okazaki I, Hiai H, Kakazu N, Yamada S, Muramatsu M, Kinoshita K, et al. Constitutive expression of AID leads to tumorigenesis. J Exp Med. 2003;197:1173-81 pubmed
product information
Product Type :
Antibody
Product Name :
CD279 (PD-1) Monoclonal Antibody (J43), Functional Grade, eBioscience
Catalog # :
16-9985-85
Quantity :
500 µg
Price :
US 429
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Armenian Hamster
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.5 µg/test, Functional Assay: Assay-Dependent
Species :
Mouse
Clone :
J43
Isotype :
IgG
Storage :
4° C
Description :
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test, Functional Assay: Assay-Dependent
Aliases :
CD279; EGK_05005; hPD1; hPD-1; hPD-l; hSLE1; Ly101; mPD-1; PD1; PD-1; Pdc1; Pdcd1; programmed cell death 1; programmed cell death 1 protein; programmed cell death protein 1; programmed cell death protein 1-like; programmed death 1; Protein PD1; protein PD-1; sCD279; SLEB2; soluble CD279; systemic lupus erythematosus susceptibility 2
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA