This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD284 (TLR4) Monoclonal Antibody (HTA125), Functional Grade, eBioscience
catalog :
16-9917-025
quantity :
25 mg
price :
US 8250
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
HTA125
reactivity :
human
application :
neutralization, flow cytometry
citations: 42
Reference
Wen S, Hong Z, Chen C, Chang H, Fu H. Helicobacter pylori Neutrophil-Activating Protein Directly Interacts with and Activates Toll-like Receptor 2 to Induce the Secretion of Interleukin-8 from Neutrophils and ATRA-Induced Differentiated HL-60 Cells. Int J Mol Sci. 2021;22: pubmed publisher
Yang Y, Hu Y, Zhou Y, Liang T, Tang H, Ju H, et al. Lys694Arg polymorphism leads to blunted responses to LPS by interfering TLR4 with recruitment of MyD88. Innate Immun. 2020;:1753425920927479 pubmed publisher
Quirino Teixeira A, Rozini S, Barbosa Lima G, Coelho D, Carneiro P, Mohana Borges R, et al. Inflammatory signaling in dengue-infected platelets requires translation and secretion of nonstructural protein 1. Blood Adv. 2020;4:2018-2031 pubmed publisher
Suzuki K, Ohkuma M, Nagaoka I. Bacterial lipopolysaccharide and antimicrobial LL-37 enhance ICAM-1 expression and NF-κB p65 phosphorylation in senescent endothelial cells. Int J Mol Med. 2019;44:1187-1196 pubmed publisher
Xiao T, Wu S, Yan C, Zhao C, Jin H, Yan N, et al. Butyrate upregulates the TLR4 expression and the phosphorylation of MAPKs and NK-κB in colon cancer cell in vitro. Oncol Lett. 2018;16:4439-4447 pubmed publisher
Hamon R, Tran H, Roscioli E, Ween M, Jersmann H, Hodge S. Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function. Sci Rep. 2018;8:13424 pubmed publisher
Bartlett D, Willis L, Slentz C, Hoselton A, KELLY L, Huebner J, et al. Ten weeks of high-intensity interval walk training is associated with reduced disease activity and improved innate immune function in older adults with rheumatoid arthritis: a pilot study. Arthritis Res Ther. 2018;20:127 pubmed publisher
Li X, Ding M, Ding C, Li L, Feng J, Yu X. Toll‑Like receptor 4 promotes the phosphorylation of CRMP2 via the activation of Rho‑kinase in MCAO rats. Mol Med Rep. 2018;18:342-348 pubmed publisher
Tobouti P, BOLT R, Radhakrishnan R, de Sousa S, Hunter K. Altered Toll-like receptor expression and function in HPV-associated oropharyngeal carcinoma. Oncotarget. 2018;9:236-248 pubmed publisher
Wu B, Wang L, Jiang L, Dong L, Xu F, Lu Y, et al. n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils. Mol Med Rep. 2017;: pubmed publisher
Bi J, Koivisto L, Pang A, Li M, Jiang G, Aurora S, et al. Suppression of αvβ6 Integrin Expression by Polymicrobial Oral Biofilms in Gingival Epithelial Cells. Sci Rep. 2017;7:4411 pubmed publisher
Trugilho M, Hottz E, Brunoro G, Teixeira Ferreira A, Carvalho P, Salazar G, et al. Platelet proteome reveals novel pathways of platelet activation and platelet-mediated immunoregulation in dengue. PLoS Pathog. 2017;13:e1006385 pubmed publisher
Li Q, Bao F, Zhi D, Liu M, Yan Q, Zheng X, et al. Lipopolysaccharide induces SBD-1 expression via the P38 MAPK signaling pathway in ovine oviduct epithelial cells. Lipids Health Dis. 2016;15:127 pubmed publisher
Lindsay S, Johnstone S, McGrath M, Mallinson D, Barnett S. Comparative miRNA-Based Fingerprinting Reveals Biological Differences in Human Olfactory Mucosa- and Bone-Marrow-Derived Mesenchymal Stromal Cells. Stem Cell Reports. 2016;6:729-742 pubmed publisher
Shi R, Wang Q, Ouyang Y, Wang Q, Xiong X. Picfeltarraenin IA inhibits lipopolysaccharide-induced inflammatory cytokine production by the nuclear factor-κB pathway in human pulmonary epithelial A549 cells. Oncol Lett. 2016;11:1195-1200 pubmed
Rossi O, Caboni M, Negrea A, Necchi F, Alfini R, Micoli F, et al. Toll-Like Receptor Activation by Generalized Modules for Membrane Antigens from Lipid A Mutants of Salmonella enterica Serovars Typhimurium and Enteritidis. Clin Vaccine Immunol. 2016;23:304-14 pubmed publisher
Avlas O, Bragg A, Fuks A, Nicholson J, Farkash A, Porat E, et al. TLR4 Expression Is Associated with Left Ventricular Dysfunction in Patients Undergoing Coronary Artery Bypass Surgery. PLoS ONE. 2015;10:e0120175 pubmed publisher
Johnston J, Nickerson J, Daroszewski J, Mogg T, Burton G. Biologically active polymers from spontaneous carotenoid oxidation: a new frontier in carotenoid activity. PLoS ONE. 2014;9:e111346 pubmed publisher
Liaunardy Jopeace A, Bryant C, Gay N. The COP II adaptor protein TMED7 is required to initiate and mediate the delivery of TLR4 to the plasma membrane. Sci Signal. 2014;7:ra70 pubmed publisher
Nogueira A, Nokhbehsaim M, Eick S, Bourauel C, Jager A, Jepsen S, et al. Biomechanical loading modulates proinflammatory and bone resorptive mediators in bacterial-stimulated PDL cells. Mediators Inflamm. 2014;2014:425421 pubmed publisher
Bretz N, Ridinger J, Rupp A, Rimbach K, Keller S, Rupp C, et al. Body fluid exosomes promote secretion of inflammatory cytokines in monocytic cells via Toll-like receptor signaling. J Biol Chem. 2013;288:36691-702 pubmed publisher
Nazli A, Kafka J, Ferreira V, Anipindi V, Mueller K, Osborne B, et al. HIV-1 gp120 induces TLR2- and TLR4-mediated innate immune activation in human female genital epithelium. J Immunol. 2013;191:4246-58 pubmed publisher
Velegraki M, Papakonstanti E, Mavroudi I, Psyllaki M, Tsatsanis C, Oulas A, et al. Impaired clearance of apoptotic cells leads to HMGB1 release in the bone marrow of patients with myelodysplastic syndromes and induces TLR4-mediated cytokine production. Haematologica. 2013;98:1206-15 pubmed publisher
Deghmane A, El Kafsi H, Giorgini D, Abaza A, Taha M. Late repression of NF-?B activity by invasive but not non-invasive meningococcal isolates is required to display apoptosis of epithelial cells. PLoS Pathog. 2011;7:e1002403 pubmed publisher
Chávez Galán L, Sada Ovalle I, Báez Saldaña R, Chavez R, Lascurain R. Monocytes from tuberculosis patients that exhibit cleaved caspase 9 and denaturalized cytochrome c are more susceptible to death mediated by Toll-like receptor 2. Immunology. 2012;135:299-311 pubmed publisher
Geraghty P, Dabo A, D ARMIENTO J. TLR4 protein contributes to cigarette smoke-induced matrix metalloproteinase-1 (MMP-1) expression in chronic obstructive pulmonary disease. J Biol Chem. 2011;286:30211-8 pubmed publisher
Lv B, Wang H, Tang Y, Fan Z, Xiao X, Chen F. High-mobility group box 1 protein induces tissue factor expression in vascular endothelial cells via activation of NF-kappaB and Egr-1. Thromb Haemost. 2009;102:352-9 pubmed publisher
Hashimoto K, Jayachandran M, Owen W, Miller V. Aggregation and microparticle production through toll-like receptor 4 activation in platelets from recently menopausal women. J Cardiovasc Pharmacol. 2009;54:57-62 pubmed publisher
Krüger B, Krick S, Dhillon N, Lerner S, Ames S, Bromberg J, et al. Donor Toll-like receptor 4 contributes to ischemia and reperfusion injury following human kidney transplantation. Proc Natl Acad Sci U S A. 2009;106:3390-5 pubmed publisher
Dasu M, Riosvelasco A, Jialal I. Candesartan inhibits Toll-like receptor expression and activity both in vitro and in vivo. Atherosclerosis. 2009;202:76-83 pubmed publisher
Schierloh P, Yokobori N, Aleman M, Landoni V, Geffner L, Musella R, et al. Mycobacterium tuberculosis-induced gamma interferon production by natural killer cells requires cross talk with antigen-presenting cells involving Toll-like receptors 2 and 4 and the mannose receptor in tuberculous pleurisy. Infect Immun. 2007;75:5325-37 pubmed
de Graaf R, Kloppenburg G, Kitslaar P, Bruggeman C, Stassen F. Human heat shock protein 60 stimulates vascular smooth muscle cell proliferation through Toll-like receptors 2 and 4. Microbes Infect. 2006;8:1859-65 pubmed
Regueiro V, Campos M, Pons J, Alberti S, Bengoechea J. The uptake of a Klebsiella pneumoniae capsule polysaccharide mutant triggers an inflammatory response by human airway epithelial cells. Microbiology. 2006;152:555-66 pubmed
MacRedmond R, Greene C, Taggart C, McElvaney N, O Neill S. Respiratory epithelial cells require Toll-like receptor 4 for induction of human beta-defensin 2 by lipopolysaccharide. Respir Res. 2005;6:116 pubmed
Vermi W, Facchetti F, Riboldi E, Heine H, Scutera S, Stornello S, et al. Role of dendritic cell-derived CXCL13 in the pathogenesis of Bartonella henselae B-rich granuloma. Blood. 2006;107:454-62 pubmed
Nagy I, Pivarcsi A, Koreck A, Szell M, Urban E, Kemeny L. Distinct strains of Propionibacterium acnes induce selective human beta-defensin-2 and interleukin-8 expression in human keratinocytes through toll-like receptors. J Invest Dermatol. 2005;124:931-8 pubmed
Chen D, Texada D, Duggan C, Liang C, Reden T, Kooragayala L, et al. Surface calreticulin mediates muramyl dipeptide-induced apoptosis in RK13 cells. J Biol Chem. 2005;280:22425-36 pubmed
Gervassi A, Alderson M, Suchland R, Maisonneuve J, Grabstein K, Probst P. Differential regulation of inflammatory cytokine secretion by human dendritic cells upon Chlamydia trachomatis infection. Infect Immun. 2004;72:7231-9 pubmed
Leifer C, Kennedy M, Mazzoni A, Lee C, Kruhlak M, Segal D. TLR9 is localized in the endoplasmic reticulum prior to stimulation. J Immunol. 2004;173:1179-83 pubmed
Mirlashari M, Lyberg T. Expression and involvement of Toll-like receptors (TLR)2, TLR4, and CD14 in monocyte TNF-alpha production induced by lipopolysaccharides from Neisseria meningitidis. Med Sci Monit. 2003;9:BR316-24 pubmed
Tabeta K, Yamazaki K, Akashi S, Miyake K, Kumada H, Umemoto T, et al. Toll-like receptors confer responsiveness to lipopolysaccharide from Porphyromonas gingivalis in human gingival fibroblasts. Infect Immun. 2000;68:3731-5 pubmed
Shimazu R, Akashi S, Ogata H, Nagai Y, Fukudome K, Miyake K, et al. MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med. 1999;189:1777-82 pubmed
product information
Product Type :
Antibody
Product Name :
CD284 (TLR4) Monoclonal Antibody (HTA125), Functional Grade, eBioscience
Catalog # :
16-9917-025
Quantity :
25 mg
Price :
US 8250
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 1 µg/test, Functional Assay: Assay-Dependent, Neutralization: Assay-Dependent
Species :
Human
Clone :
HTA125
Isotype :
IgG2a, kappa
Storage :
4° C
Description :
TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1 µg/test, Functional Assay: Assay-Dependent, Neutralization: Assay-Dependent
Aliases :
ARMD10; CD284; EGK_07381; homolog of Drosophila toll; hToll; lipopolysaccharide response; Lps; Ly87; Ran/M1; Rasl2-8; Tlr4; TLR-4; TOLL; toll like receptor 4; toll/interleukin-l receptor (TIR) domain; toll4; toll-like receptor 4; toll-like receptor 4 immunity-related protein; Toll-like receptor 4-like protein; Toll-like receptor4 protein
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA