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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
TNF alpha Monoclonal Antibody (MAb1), Functional Grade, eBioscience
catalog :
16-7348-81
quantity :
50 µg
price :
US 133
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MAb1
reactivity :
human
application :
ELISA, neutralization, flow cytometry, blocking or activating experiments
more info or order :
Invitrogen product webpage
citations: 30
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; fig s2c
Wang Y, Zhong H, Xie X, Chen C, Huang D, Shen L, et al. Long noncoding RNA derived from CD244 signaling epigenetically controls CD8+ T-cell immune responses in tuberculosis infection. Proc Natl Acad Sci U S A. 2015;112:E3883-92 pubmed publisher
  • blocking or activating experiments; human; 10 ug/ml; fig 3
Tsai C, Liong K, Gunalan M, Li N, Lim D, Fisher D, et al. Type I IFNs and IL-18 regulate the antiviral response of primary human γδ T cells against dendritic cells infected with Dengue virus. J Immunol. 2015;194:3890-900 pubmed publisher
  • ELISA; human; 1:500
Vogelpoel L, Hansen I, Rispens T, Muller F, van Capel T, Turina M, et al. Fc gamma receptor-TLR cross-talk elicits pro-inflammatory cytokine production by human M2 macrophages. Nat Commun. 2014;5:5444 pubmed publisher
  • blocking or activating experiments; human; 5 ug/ml
Lim D, Yawata N, Selva K, Li N, Tsai C, Yeong L, et al. The combination of type I IFN, TNF-α, and cell surface receptor engagement with dendritic cells enables NK cells to overcome immune evasion by dengue virus. J Immunol. 2014;193:5065-75 pubmed publisher
  • flow cytometry; human; 1 ug/ml; fig 3
Nagy L, Grishina I, Macal M, Hirao L, Hu W, Sankaran Walters S, et al. Chronic HIV infection enhances the responsiveness of antigen presenting cells to commensal Lactobacillus. PLoS ONE. 2013;8:e72789 pubmed publisher
Birkl D, Quirós M, García Hernández V, Zhou D, Brazil J, Hilgarth R, et al. TNFα promotes mucosal wound repair through enhanced platelet activating factor receptor signaling in the epithelium. Mucosal Immunol. 2019;: pubmed publisher
Liu W, Sun Y, He Y, Zhang H, Zheng Y, Yao Y, et al. IL-1? impedes the chondrogenic differentiation of synovial fluid mesenchymal stem cells in the human temporomandibular joint. Int J Mol Med. 2017;39:317-326 pubmed publisher
Sullivan J, Jankowska Gan E, Hegde S, Pestrak M, Agashe V, Park A, et al. Th17 Responses to Collagen Type V, kα1-Tubulin, and Vimentin Are Present Early in Human Development and Persist Throughout Life. Am J Transplant. 2017;17:944-956 pubmed publisher
Hristodorov D, Mladenov R, Brehm H, Fischer R, Barth S, Thepen T. Recombinant H22(scFv) blocks CD64 and prevents the capture of anti-TNF monoclonal antibody. A potential strategy to enhance anti-TNF therapy. MAbs. 2014;6:1283-9 pubmed publisher
Jayaraman P, Sada Ovalle I, Nishimura T, Anderson A, Kuchroo V, Remold H, et al. IL-1? promotes antimicrobial immunity in macrophages by regulating TNFR signaling and caspase-3 activation. J Immunol. 2013;190:4196-204 pubmed publisher
Gellatly S, Needham B, Madera L, Trent M, Hancock R. The Pseudomonas aeruginosa PhoP-PhoQ two-component regulatory system is induced upon interaction with epithelial cells and controls cytotoxicity and inflammation. Infect Immun. 2012;80:3122-31 pubmed publisher
Stegmann K, Björkström N, Ciesek S, Lunemann S, Jaroszewicz J, Wiegand J, et al. Interferon ?-stimulated natural killer cells from patients with acute hepatitis C virus (HCV) infection recognize HCV-infected and uninfected hepatoma cells via DNAX accessory molecule-1. J Infect Dis. 2012;205:1351-62 pubmed publisher
Vohra P, Poxton I. Induction of cytokines in a macrophage cell line by proteins of Clostridium difficile. FEMS Immunol Med Microbiol. 2012;65:96-104 pubmed publisher
Nijnik A, Pistolic J, Cho P, Filewod N, Falsafi R, Ramin A, et al. The role of the Src family kinase Lyn in the immunomodulatory activities of cathelicidin peptide LL-37 on monocytic cells. J Leukoc Biol. 2012;91:599-607 pubmed publisher
Lakhashe S, Wang W, Siddappa N, Hemashettar G, Polacino P, Hu S, et al. Vaccination against heterologous R5 clade C SHIV: prevention of infection and correlates of protection. PLoS ONE. 2011;6:e22010 pubmed publisher
Ryba M, Marek N, Hak Ł, Rybarczyk Kapturska K, Myśliwiec M, Trzonkowski P, et al. Anti-TNF rescue CD4+Foxp3+ regulatory T cells in patients with type 1 diabetes from effects mediated by TNF. Cytokine. 2011;55:353-61 pubmed publisher
Li W, Katz B, Spinola S. Haemophilus ducreyi lipooligosaccharides induce expression of the immunosuppressive enzyme indoleamine 2,3-dioxygenase via type I interferons and tumor necrosis factor alpha in human dendritic cells. Infect Immun. 2011;79:3338-47 pubmed publisher
Nijnik A, Pistolic J, Wyatt A, Tam S, Hancock R. Human cathelicidin peptide LL-37 modulates the effects of IFN-gamma on APCs. J Immunol. 2009;183:5788-98 pubmed publisher
Matheson L, Duong T, Rosenberg A, Yeung R. Assessment of sample collection and storage methods for multicenter immunologic research in children. J Immunol Methods. 2008;339:82-9 pubmed publisher
Caminschi I, Proietto A, Ahmet F, Kitsoulis S, Shin Teh J, Lo J, et al. The dendritic cell subtype-restricted C-type lectin Clec9A is a target for vaccine enhancement. Blood. 2008;112:3264-73 pubmed publisher
Ma C, Chew G, Simpson N, Priyadarshi A, Wong M, Grimbacher B, et al. Deficiency of Th17 cells in hyper IgE syndrome due to mutations in STAT3. J Exp Med. 2008;205:1551-7 pubmed publisher
Reske A, Pollara G, Krummenacher C, Katz D, Chain B. Glycoprotein-dependent and TLR2-independent innate immune recognition of herpes simplex virus-1 by dendritic cells. J Immunol. 2008;180:7525-36 pubmed
Inglefield J, Dumitru C, Alkan S, Gibson S, Lipson K, Tomai M, et al. TLR7 agonist 852A inhibition of tumor cell proliferation is dependent on plasmacytoid dendritic cells and type I IFN. J Interferon Cytokine Res. 2008;28:253-63 pubmed publisher
Erridge C, Attina T, Spickett C, Webb D. A high-fat meal induces low-grade endotoxemia: evidence of a novel mechanism of postprandial inflammation. Am J Clin Nutr. 2007;86:1286-92 pubmed
Nishikawa H, Tsuji T, Jager E, Briones G, Ritter G, Old L, et al. Induction of regulatory T cell-resistant helper CD4+ T cells by bacterial vector. Blood. 2008;111:1404-12 pubmed
Kobayashi R, Okamura S, Ohno T, Saito H, Mori M, Ra C, et al. Hyperexpression of FcgammaRI and Toll-like receptor 4 in the intestinal mast cells of Crohn's disease patients. Clin Immunol. 2007;125:149-58 pubmed
Gorski K, Waller E, Bjornton Severson J, Hanten J, Riter C, Kieper W, et al. Distinct indirect pathways govern human NK-cell activation by TLR-7 and TLR-8 agonists. Int Immunol. 2006;18:1115-26 pubmed
Nagaoka I, Tamura H, Hirata M. An antimicrobial cathelicidin peptide, human CAP18/LL-37, suppresses neutrophil apoptosis via the activation of formyl-peptide receptor-like 1 and P2X7. J Immunol. 2006;176:3044-52 pubmed
Barchet W, Price J, Cella M, Colonna M, MacMillan S, Cobb J, et al. Complement-induced regulatory T cells suppress T-cell responses but allow for dendritic-cell maturation. Blood. 2006;107:1497-504 pubmed
Gavin I, Kukhtin A, Glesne D, Schabacker D, Chandler D. Analysis of protein interaction and function with a 3-dimensional MALDI-MS protein array. Biotechniques. 2005;39:99-107 pubmed
product information
Product Type :
Antibody
Product Name :
TNF alpha Monoclonal Antibody (MAb1), Functional Grade, eBioscience
Catalog # :
16-7348-81
Quantity :
50 µg
Price :
US 133
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
ELISA: Assay-Dependent, Functional Assay: Assay-Dependent, Neutralization: Assay-Dependent
Species :
Human
Clone :
MAb1
Isotype :
IgG1, kappa
Storage :
4° C
Description :
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-Dependent, Functional Assay: Assay-Dependent, Neutralization: Assay-Dependent
Aliases :
APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA