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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
IFN gamma Monoclonal Antibody (AN-18), Functional Grade, eBioscience
catalog :
16-7313-85
quantity :
500 µg
price :
US 353
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
AN-18
reactivity :
mouse
application :
ELISA, flow cytometry
more info or order :
citations: 68
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 2f, 3c,
Raphael I, Gomez Rivera F, Raphael R, Robinson R, Nalawade S, Forsthuber T. TNFR2 limits proinflammatory astrocyte functions during EAE induced by pathogenic DR2b-restricted T cells. JCI Insight. 2019;4: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4d
Yang X, Lin Y, Shi Y, Li B, Liu W, Yin W, et al. FAP Promotes Immunosuppression by Cancer-Associated Fibroblasts in the Tumor Microenvironment via STAT3-CCL2 Signaling. Cancer Res. 2016;76:4124-35 pubmed publisher
  • ELISA; mouse; fig 3D
Arellano B, Hussain R, Miller Little W, Herndon E, Lambracht Washington D, Eagar T, et al. A Single Amino Acid Substitution Prevents Recognition of a Dominant Human Aquaporin-4 Determinant in the Context of HLA-DRB1*03:01 by a Murine TCR. PLoS ONE. 2016;11:e0152720 pubmed publisher
Miranda Katz M, Erickson J, Lan J, Ecker A, Zhang Y, Joyce S, et al. Novel HLA-B7-restricted human metapneumovirus epitopes enhance viral clearance in mice and are recognized by human CD8+ T cells. Sci Rep. 2021;11:20769 pubmed publisher
Morelli A, Sumpter T, Rojas Canales D, Bandyopadhyay M, Chen Z, Tkacheva O, et al. Neurokinin-1 Receptor Signaling Is Required for Efficient Ca2+ Flux in T-Cell-Receptor-Activated T Cells. Cell Rep. 2020;30:3448-3465.e8 pubmed publisher
Kwak J, Kim Y, Park S, Yu M, Kwon H, Eo S, et al. Development of a SFTSV DNA vaccine that confers complete protection against lethal infection in ferrets. Nat Commun. 2019;10:3836 pubmed publisher
Gibson H, Munns S, FREYTAG S, Barton K, Veenstra J, Bettahi I, et al. Immunotherapeutic intervention with oncolytic adenovirus in mouse mammary tumors. Oncoimmunology. 2015;4:e984523 pubmed
Tsou Y, Lin Y, Shao H, Yu S, Wu S, Lin H, et al. Recombinant adeno-vaccine expressing enterovirus 71-like particles against hand, foot, and mouth disease. PLoS Negl Trop Dis. 2015;9:e0003692 pubmed publisher
Kim S, Chung H, Lee K, Lim J. Identification of novel epitopes from human papillomavirus type 18 E7 that can sensitize PBMCs of multiple HLA class I against human cervical cancer. J Transl Med. 2014;12:229 pubmed publisher
Lee J, Kim J, Lee J, Shin S, Shin E. DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B elicits polyfunctional CD8(+) T cell responses. Clin Exp Vaccine Res. 2014;3:235-43 pubmed publisher
Wagage S, John B, Krock B, Hall A, Randall L, Karp C, et al. The aryl hydrocarbon receptor promotes IL-10 production by NK cells. J Immunol. 2014;192:1661-70 pubmed publisher
Sosa R, Murphey C, Ji N, Cardona A, Forsthuber T. The kinetics of myelin antigen uptake by myeloid cells in the central nervous system during experimental autoimmune encephalomyelitis. J Immunol. 2013;191:5848-57 pubmed publisher
Ji N, Somanaboeina A, Dixit A, Kawamura K, Hayward N, Self C, et al. Small molecule inhibitor of antigen binding and presentation by HLA-DR2b as a therapeutic strategy for the treatment of multiple sclerosis. J Immunol. 2013;191:5074-84 pubmed publisher
Batoulis H, Recks M, Holland F, Thomalla F, Williams R, Kuerten S. Blockade of tumour necrosis factor-? in experimental autoimmune encephalomyelitis reveals differential effects on the antigen-specific immune response and central nervous system histopathology. Clin Exp Immunol. 2014;175:41-8 pubmed publisher
Jaini R, Rayman P, Cohen P, Finke J, Tuohy V. Combination of sunitinib with anti-tumor vaccination inhibits T cell priming and requires careful scheduling to achieve productive immunotherapy. Int J Cancer. 2014;134:1695-705 pubmed publisher
Li W, Murthy A, Lanka G, Chetty S, Yu J, Chambers J, et al. A T cell epitope-based vaccine protects against chlamydial infection in HLA-DR4 transgenic mice. Vaccine. 2013;31:5722-8 pubmed publisher
Kuerten S, Wunsch M, Lehmann P. Longitudinal T cell-derived IFN-?/IL-17 balances do not correlate with the disease course in two mouse models of experimental autoimmune encephalomyelitis. J Immunol Methods. 2013;398-399:68-75 pubmed publisher
Monk J, Hou T, Turk H, McMurray D, Chapkin R. n3 PUFAs reduce mouse CD4+ T-cell ex vivo polarization into Th17 cells. J Nutr. 2013;143:1501-8 pubmed publisher
Garcia J, Pino P, Mizutani M, Cardona S, Charo I, Ransohoff R, et al. Regulation of adaptive immunity by the fractalkine receptor during autoimmune inflammation. J Immunol. 2013;191:1063-72 pubmed publisher
Ryan S, Johnson J, Cobb B. Neutrophils confer T cell resistance to myeloid-derived suppressor cell-mediated suppression to promote chronic inflammation. J Immunol. 2013;190:5037-47 pubmed publisher
Gilchuk P, Spencer C, Conant S, Hill T, Gray J, Niu X, et al. Discovering naturally processed antigenic determinants that confer protective T cell immunity. J Clin Invest. 2013;123:1976-87 pubmed publisher
Blum L, Mohanan S, Fabre M, Yafawi R, Appleton J. Intestinal infection with Trichinella spiralis induces distinct, regional immune responses. Vet Parasitol. 2013;194:101-5 pubmed publisher
Scalfone L, Nel H, Gagliardo L, Cameron J, Al Shokri S, Leifer C, et al. Participation of MyD88 and interleukin-33 as innate drivers of Th2 immunity to Trichinella spiralis. Infect Immun. 2013;81:1354-63 pubmed publisher
Kozak K, Wang J, Lye M, Chuh J, Takkar R, Kim N, et al. Micro-volume wall-less immunoassays using patterned planar plates. Lab Chip. 2013;13:1342-50 pubmed publisher
Hundgeburth L, Wunsch M, Rovituso D, Recks M, Addicks K, Lehmann P, et al. The complement system contributes to the pathology of experimental autoimmune encephalomyelitis by triggering demyelination and modifying the antigen-specific T and B cell response. Clin Immunol. 2013;146:155-64 pubmed publisher
Monk J, Hou T, Turk H, Weeks B, Wu C, McMurray D, et al. Dietary n-3 polyunsaturated fatty acids (PUFA) decrease obesity-associated Th17 cell-mediated inflammation during colitis. PLoS ONE. 2012;7:e49739 pubmed publisher
Young E, Vichaya E, Reusser N, Cook J, Steelman A, Welsh C, et al. Chronic social stress impairs virus specific adaptive immunity during acute Theiler's virus infection. J Neuroimmunol. 2013;254:19-27 pubmed publisher
Hall A, Beiting D, Tato C, John B, Oldenhove G, Lombana C, et al. The cytokines interleukin 27 and interferon-? promote distinct Treg cell populations required to limit infection-induced pathology. Immunity. 2012;37:511-23 pubmed publisher
Weinger J, Weist B, Plaisted W, Klaus S, Walsh C, Lane T. MHC mismatch results in neural progenitor cell rejection following spinal cord transplantation in a model of viral-induced demyelination. Stem Cells. 2012;30:2584-95 pubmed publisher
Kuerten S, Schickel A, Kerkloh C, Recks M, Addicks K, Ruddle N, et al. Tertiary lymphoid organ development coincides with determinant spreading of the myelin-specific T cell response. Acta Neuropathol. 2012;124:861-73 pubmed publisher
Batoulis H, Uhl M, Addicks K, Lehmann P, Kuerten S. The magnitude of the antigen-specific T cell response is separated from the severity of spinal cord histopathology in remitting-relapsing experimental autoimmune encephalomyelitis. Glia. 2012;60:794-805 pubmed publisher
Boué J, Blanpied C, Djata Cabral M, Pelletier L, Vergnolle N, Dietrich G. Immune conditions associated with CD4+ T effector-induced opioid release and analgesia. Pain. 2012;153:485-93 pubmed publisher
Massilamany C, Asojo O, Gangaplara A, Steffen D, Reddy J. Identification of a second mimicry epitope from Acanthamoeba castellanii that induces CNS autoimmunity by generating cross-reactive T cells for MBP 89-101 in SJL mice. Int Immunol. 2011;23:729-39 pubmed publisher
Massilamany C, Gangaplara A, Steffen D, Reddy J. Identification of novel mimicry epitopes for cardiac myosin heavy chain-? that induce autoimmune myocarditis in A/J mice. Cell Immunol. 2011;271:438-49 pubmed publisher
Lee D, Kim S, Kang W, Choi Y, Lee S, Lee S, et al. Adjuvant effect of bacterial outer membrane vesicles with penta-acylated lipopolysaccharide on antigen-specific T cell priming. Vaccine. 2011;29:8293-301 pubmed publisher
Collins E, Jager L, Dabelic R, Benitez P, Holdstein K, Lau K, et al. Inhibition of SOCS1-/- lethal autoinflammatory disease correlated to enhanced peripheral Foxp3+ regulatory T cell homeostasis. J Immunol. 2011;187:2666-76 pubmed publisher
Paterson A, Brown K, Keir M, Vanguri V, Riella L, Chandraker A, et al. The programmed death-1 ligand 1:B7-1 pathway restrains diabetogenic effector T cells in vivo. J Immunol. 2011;187:1097-105 pubmed publisher
Rottlaender A, Villwock H, Addicks K, Kuerten S. Neuroprotective role of fibroblast growth factor-2 in experimental autoimmune encephalomyelitis. Immunology. 2011;133:370-8 pubmed publisher
Javeri S, Rodi M, Tary Lehmann M, Lehmann P, Addicks K, Kuerten S. Involvement of brain-derived neurotrophic factor (BDNF) in MP4-induced autoimmune encephalomyelitis. Clin Immunol. 2010;137:181-9 pubmed publisher
Gürlevik E, Woller N, Strüver N, Schache P, Kloos A, Manns M, et al. Selectivity of oncolytic viral replication prevents antiviral immune response and toxicity, but does not improve antitumoral immunity. Mol Ther. 2010;18:1972-82 pubmed publisher
Chan P, Liu S, Cheung T, Yeo W, Ngai S, Cheung J, et al. T-cell response to human papillomavirus type 58 L1, E6, And E7 peptides in women with cleared infection, cervical intraepithelial neoplasia, or invasive cancer. Clin Vaccine Immunol. 2010;17:1315-21 pubmed publisher
Li W, Murthy A, Guentzel M, Chambers J, Forsthuber T, Seshu J, et al. Immunization with a combination of integral chlamydial antigens and a defined secreted protein induces robust immunity against genital chlamydial challenge. Infect Immun. 2010;78:3942-9 pubmed publisher
Nesbeth Y, Martinez D, Toraya S, Scarlett U, Cubillos Ruiz J, Rutkowski M, et al. CD4+ T cells elicit host immune responses to MHC class II-negative ovarian cancer through CCL5 secretion and CD40-mediated licensing of dendritic cells. J Immunol. 2010;184:5654-62 pubmed publisher
Noor S, Habashy A, Nance J, Clark R, Nemati K, Carson M, et al. CCR7-dependent immunity during acute Toxoplasma gondii infection. Infect Immun. 2010;78:2257-63 pubmed publisher
Kuerten S, Rodi M, Javeri S, Gruppe T, Tary Lehmann M, Lehmann P, et al. Delineating the impact of neuroantigen vs genetic diversity on MP4-induced EAE of C57BL/6 and B6.129 mice. APMIS. 2009;117:923-35 pubmed publisher
Narazaki H, Zhu Y, Luo L, Zhu G, Chen L. CD137 agonist antibody prevents cancer recurrence: contribution of CD137 on both hematopoietic and nonhematopoietic cells. Blood. 2010;115:1941-8 pubmed publisher
Massilamany C, Steffen D, Reddy J. An epitope from Acanthamoeba castellanii that cross-react with proteolipid protein 139-151-reactive T cells induces autoimmune encephalomyelitis in SJL mice. J Neuroimmunol. 2010;219:17-24 pubmed publisher
Burrows M, Assundani D, Celis E, Tufaro F, Tanaka A, Bradley W. Oral administration of PPC enhances antigen-specific CD8+ T cell responses while reducing IgE levels in sensitized mice. BMC Complement Altern Med. 2009;9:49 pubmed publisher
Tigno Aranjuez J, Jaini R, Tuohy V, Lehmann P, Tary Lehmann M. Encephalitogenicity of complete Freund's adjuvant relative to CpG is linked to induction of Th17 cells. J Immunol. 2009;183:5654-61 pubmed publisher
Fanning S, Appel M, Berger S, Korngold R, Friedman T. The immunological impact of genetic drift in the B10.BR congenic inbred mouse strain. J Immunol. 2009;183:4261-72 pubmed publisher
Cubillos Ruiz J, Engle X, Scarlett U, Martinez D, Barber A, Elgueta R, et al. Polyethylenimine-based siRNA nanocomplexes reprogram tumor-associated dendritic cells via TLR5 to elicit therapeutic antitumor immunity. J Clin Invest. 2009;119:2231-44 pubmed publisher
Steelman A, Dean D, Young C, Smith R, Prentice T, Meagher M, et al. Restraint stress modulates virus specific adaptive immunity during acute Theiler's virus infection. Brain Behav Immun. 2009;23:830-43 pubmed publisher
Bai L, Lennon D, Eaton V, Maier K, Caplan A, Miller S, et al. Human bone marrow-derived mesenchymal stem cells induce Th2-polarized immune response and promote endogenous repair in animal models of multiple sclerosis. Glia. 2009;57:1192-203 pubmed publisher
Edukulla R, Ramakrishna E, Woller N, Mundt B, Knocke S, Gürlevik E, et al. Antitumoral immune response by recruitment and expansion of dendritic cells in tumors infected with telomerase-dependent oncolytic viruses. Cancer Res. 2009;69:1448-58 pubmed publisher
Whitlock G, Lukaszewski R, Judy B, Paessler S, Torres A, Estes D. Host immunity in the protective response to vaccination with heat-killed Burkholderia mallei. BMC Immunol. 2008;9:55 pubmed publisher
Huarte E, Cubillos Ruiz J, Nesbeth Y, Scarlett U, Martinez D, Buckanovich R, et al. Depletion of dendritic cells delays ovarian cancer progression by boosting antitumor immunity. Cancer Res. 2008;68:7684-91 pubmed publisher
Wegmann K, Wagner C, Whitham R, Hinrichs D. Synthetic Peptide dendrimers block the development and expression of experimental allergic encephalomyelitis. J Immunol. 2008;181:3301-9 pubmed
Kawamura K, McLaughlin K, Weissert R, Forsthuber T. Myelin-reactive type B T cells and T cells specific for low-affinity MHC-binding myelin peptides escape tolerance in HLA-DR transgenic mice. J Immunol. 2008;181:3202-11 pubmed
Hamdy S, Molavi O, Ma Z, Haddadi A, Alshamsan A, Gobti Z, et al. Co-delivery of cancer-associated antigen and Toll-like receptor 4 ligand in PLGA nanoparticles induces potent CD8+ T cell-mediated anti-tumor immunity. Vaccine. 2008;26:5046-57 pubmed publisher
Couper K, Blount D, Hafalla J, Van Rooijen N, de Souza J, Riley E. Macrophage-mediated but gamma interferon-independent innate immune responses control the primary wave of Plasmodium yoelii parasitemia. Infect Immun. 2007;75:5806-18 pubmed
Denkinger C, Denkinger M, Forsthuber T. Pertussis toxin-induced cytokine differentiation and clonal expansion of T cells is mediated predominantly via costimulation. Cell Immunol. 2007;246:46-54 pubmed
Grigorian A, Lee S, Tian W, Chen I, Gao G, Mendelsohn R, et al. Control of T Cell-mediated autoimmunity by metabolite flux to N-glycan biosynthesis. J Biol Chem. 2007;282:20027-35 pubmed
Hofstetter H, Kovalovsky A, Shive C, Lehmann P, Forsthuber T. Neonatal induction of myelin-specific Th1/Th17 immunity does not result in experimental autoimmune encephalomyelitis and can protect against the disease in adulthood. J Neuroimmunol. 2007;187:20-30 pubmed
Malboeuf C, Simon D, Lee Y, Lankes H, Dewhurst S, Frelinger J, et al. Human papillomavirus-like particles mediate functional delivery of plasmid DNA to antigen presenting cells in vivo. Vaccine. 2007;25:3270-6 pubmed
Liang S, Tan X, Luxenberg D, Karim R, Dunussi Joannopoulos K, Collins M, et al. Interleukin (IL)-22 and IL-17 are coexpressed by Th17 cells and cooperatively enhance expression of antimicrobial peptides. J Exp Med. 2006;203:2271-9 pubmed
Kuerten S, Lichtenegger F, Faas S, Angelov D, Tary Lehmann M, Lehmann P. MBP-PLP fusion protein-induced EAE in C57BL/6 mice. J Neuroimmunol. 2006;177:99-111 pubmed
Kort J, Kawamura K, Fugger L, Weissert R, Forsthuber T. Efficient presentation of myelin oligodendrocyte glycoprotein peptides but not protein by astrocytes from HLA-DR2 and HLA-DR4 transgenic mice. J Neuroimmunol. 2006;173:23-34 pubmed
Nekrasova T, Shive C, Gao Y, Kawamura K, Guardia R, Landreth G, et al. ERK1-deficient mice show normal T cell effector function and are highly susceptible to experimental autoimmune encephalomyelitis. J Immunol. 2005;175:2374-80 pubmed
product information
Product Type :
Antibody
Product Name :
IFN gamma Monoclonal Antibody (AN-18), Functional Grade, eBioscience
Catalog # :
16-7313-85
Quantity :
500 µg
Price :
US 353
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Control: Assay-Dependent, Functional Assay: Assay-Dependent, Immunostaining: 1-4 µg/mL
Species :
Mouse
Clone :
AN-18
Isotype :
IgG1, kappa
Storage :
4° C
Description :
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
Format :
Liquid
Applications w/Dilutions :
Control: Assay-Dependent, Functional Assay: Assay-Dependent, Immunostaining: 1-4 µg/mL
Aliases :
BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA