IL-27 p28 Monoclonal Antibody (MM27-7B1), Functional Grade, eBioscience | Invitrogen 16-7285-85 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

IL-27 p28 Monoclonal Antibody (MM27-7B1), Functional Grade, eBioscience

catalog :

16-7285-85

quantity :

500 µg

price :

US 564.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MM27-7B1

reactivity :

mouse

application :

neutralization, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; mouse; loading ...; fig 3a	Jirmo A, Daluege K, Happle C, Albrecht M, Dittrich A, Busse M, et al. IL-27 Is Essential for Suppression of Experimental Allergic Asthma by the TLR7/8 Agonist R848 (Resiquimod). J Immunol. 2016;197:4219-4227 pubmed
	Pyle C, Uwadiae F, Swieboda D, Harker J. Early IL-6 signalling promotes IL-27 dependent maturation of regulatory T cells in the lungs and resolution of viral immunopathology. PLoS Pathog. 2017;13:e1006640 pubmed publisher
	Bosmann M, Haggadone M, Hemmila M, Zetoune F, Sarma J, Ward P. Complement activation product C5a is a selective suppressor of TLR4-induced, but not TLR3-induced, production of IL-27(p28) from macrophages. J Immunol. 2012;188:5086-93 pubmed publisher

product information

Product Type :

Antibody

Product Name :

IL-27 p28 Monoclonal Antibody (MM27-7B1), Functional Grade, eBioscience

Catalog # :

16-7285-85

Quantity :

500 µg

Price :

US 564.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Mouse

Applications :

Functional Assay: Assay-Dependent, Neutralization: Assay-Dependent

Species :

Mouse

Clone :

MM27-7B1

Isotype :

IgG2a, kappa

Storage :

4° C

Description :

IL-27, a member of the IL-12 family, is a heterodimeric protein consisting of the p40-related protein Epstein-Barr virus-induced gene 3 (EBI3) non-covalently linked to an IL-12p35-related protein, p28 (also known as IL-30). IL-27 is produced by activated APCs and mature dendritic cells. IL-27 exerts its activities on NK cells and naive CD4+ T cells; mRNA expression analysis of IL-27 receptor components (WSX-1/TCCR and gp130) suggests that IL-27 may also target other cells, including mast cells and monocytes. Binding of IL-27 to WSX-1/gp130 activates JAK1, STAT1, and STAT3 and STAT1/3 phosphorylation. WSX-1/TCCR-deficient mice develop impaired Th1 responses and are more susceptible to infection with L. monocytogenes suggesting that Th1 responses require IL-27. Although activation of WSX-1 is required for the initiation of Th1 responses, it is not necessary for maintaining Th1 responses. IL-27 alone is not able to induce the differentiation of CD4+ T cells into IFN- -producing cells, suggesting a role for IL-27 as an initial activator of Th1 responses. An important effect of IL-27 in initiating Th1 responses is the induction of the Th1-specific transcription factor T-bet as well as the suppression of the Th2-specific transcription factor GATA-3. T-bet plays a critical role in Th1 differentiation by its ability to maintain IL-12R 2 expression following CD4+ T cell activation. Recent studies indicate that IL-27 has a potent antitumor activity. In vitro, IL-27 has been found to act directly on naive CD8 cells, generating CTL with enhanced granzyme B expression. In vivo, IL-27 has been reported to augment CTL activity, inhibit tumor growth, and induce complete regression of primary and metastatic neuroblastoma tumors.

Format :

Liquid

Applications w/Dilutions :

Functional Assay: Assay-Dependent, Neutralization: Assay-Dependent

Aliases :

IL27; IL-27; IL-27 p28 subunit; IL-27 subunit alpha; Il27a; IL-27A; IL-27-A; IL27-A; IL27p28; IL-27p28; IL30; IL-30; ILN; Interleukin; interleukin 27; interleukin 30; Interleukin-27; interleukin-27 subunit alpha; Interleukin-30; p28

more info or order :

Invitrogen product webpage