This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
IL-10 Monoclonal Antibody (JES5-2A5), Functional Grade, eBioscience
catalog :
16-7102-025
quantity :
25 mg
price :
US 8250
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
JES5-2A5
reactivity :
mouse
application :
neutralization
citations: 22
Published Application/Species/Sample/DilutionReference
  • ELISA; mouse; loading ...; fig 5
Akgul A, Maddaloni M, Jun S, Nelson A, Odreman V, Hoffman C, et al. Stimulation of regulatory T cells with Lactococcus lactis expressing enterotoxigenic E. coli colonization factor antigen 1 retains salivary flow in a genetic model of Sjögren's syndrome. Arthritis Res Ther. 2021;23:99 pubmed publisher
Glenn J, Smith M, Xue P, Chan Li Y, Collins S, Calabresi P, et al. CNS-targeted autoimmunity leads to increased influenza mortality in mice. J Exp Med. 2017;214:297-307 pubmed publisher
Howes A, Taubert C, Blankley S, Spink N, Wu X, Graham C, et al. Differential Production of Type I IFN Determines the Reciprocal Levels of IL-10 and Proinflammatory Cytokines Produced by C57BL/6 and BALB/c Macrophages. J Immunol. 2016;197:2838-53 pubmed publisher
Peda J, Salah S, Wallace D, Fields P, Grantham C, Fields T, et al. Autocrine IL-10 activation of the STAT3 pathway is required for pathological macrophage differentiation in polycystic kidney disease. Dis Model Mech. 2016;9:1051-61 pubmed publisher
Yao Y, Vent Schmidt J, McGeough M, Wong M, Hoffman H, Steiner T, et al. Tr1 Cells, but Not Foxp3+ Regulatory T Cells, Suppress NLRP3 Inflammasome Activation via an IL-10-Dependent Mechanism. J Immunol. 2015;195:488-97 pubmed publisher
Kashi V, Ortega S, Karandikar N. Neuroantigen-specific autoregulatory CD8+ T cells inhibit autoimmune demyelination through modulation of dendritic cell function. PLoS ONE. 2014;9:e105763 pubmed publisher
Mooney J, Butler B, Lokken K, Xavier M, Chau J, Schaltenberg N, et al. The mucosal inflammatory response to non-typhoidal Salmonella in the intestine is blunted by IL-10 during concurrent malaria parasite infection. Mucosal Immunol. 2014;7:1302-11 pubmed publisher
Toda M, Shao Z, Yamaguchi K, Takagi T, D Alessandro Gabazza C, Taguchi O, et al. Differential gene expression in thrombomodulin (TM; CD141)(+) and TM(-) dendritic cell subsets. PLoS ONE. 2013;8:e72392 pubmed publisher
He X, Berland R, Mekasha S, Christensen T, Alroy J, Kramnik I, et al. The sst1 resistance locus regulates evasion of type I interferon signaling by Chlamydia pneumoniae as a disease tolerance mechanism. PLoS Pathog. 2013;9:e1003569 pubmed publisher
Mineo S, Niikura M, Inoue S, Kuroda M, Kobayashi F. Development of severe pathology in immunized pregnant mice challenged with lethal malaria parasites. Infect Immun. 2013;81:3865-71 pubmed publisher
Mays L, Ammon Treiber S, Mothes B, Alkhaled M, Rottenberger J, Müller Hermelink E, et al. Modified Foxp3 mRNA protects against asthma through an IL-10-dependent mechanism. J Clin Invest. 2013;123:1216-28 pubmed publisher
Tang G, Zhou H, Xu J, Xu J, Ji M, Wu H, et al. Schistosoma japonicum soluble egg antigens attenuate IFN-?-induced MHC class II expression in RAW 264.7 macrophages. PLoS ONE. 2012;7:e49234 pubmed publisher
Gaddis D, Maynard C, Weaver C, Michalek S, Katz J. Role of TLR2-dependent IL-10 production in the inhibition of the initial IFN-? T cell response to Porphyromonas gingivalis. J Leukoc Biol. 2013;93:21-31 pubmed publisher
Silberman D, Bucknum A, Bartlett T, Composto G, Kozlowski M, Walker A, et al. CD28 ligation increases macrophage suppression of T-cell proliferation. Cell Mol Immunol. 2012;9:341-9 pubmed publisher
Hashimoto D, Chow A, Greter M, Saenger Y, Kwan W, Leboeuf M, et al. Pretransplant CSF-1 therapy expands recipient macrophages and ameliorates GVHD after allogeneic hematopoietic cell transplantation. J Exp Med. 2011;208:1069-82 pubmed publisher
Lajoie S, Lewkowich I, Suzuki Y, Clark J, Sproles A, Dienger K, et al. Complement-mediated regulation of the IL-17A axis is a central genetic determinant of the severity of experimental allergic asthma. Nat Immunol. 2010;11:928-35 pubmed publisher
Nijnik A, Madera L, Ma S, Waldbrook M, Elliott M, Easton D, et al. Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment. J Immunol. 2010;184:2539-50 pubmed publisher
Connolly M, Mallen St Clair J, Bedrosian A, Malhotra A, Vera V, Ibrahim J, et al. Distinct populations of metastases-enabling myeloid cells expand in the liver of mice harboring invasive and preinvasive intra-abdominal tumor. J Leukoc Biol. 2010;87:713-25 pubmed publisher
Jørgensen T, Alfaro J, Enriquez H, Jiang C, Loo W, Atencio S, et al. Development of murine lupus involves the combined genetic contribution of the SLAM and FcgammaR intervals within the Nba2 autoimmune susceptibility locus. J Immunol. 2010;184:775-86 pubmed publisher
Sheng J, Li L, Ganesh B, Prabhakar B, Meriggioli M. Regulatory T cells induced by GM-CSF suppress ongoing experimental myasthenia gravis. Clin Immunol. 2008;128:172-80 pubmed publisher
Tsuboi Y, Abe H, Nakagawa R, Oomizu S, Watanabe K, Nishi N, et al. Galectin-9 protects mice from the Shwartzman reaction by attracting prostaglandin E2-producing polymorphonuclear leukocytes. Clin Immunol. 2007;124:221-33 pubmed
Flano E, Kayhan B, Woodland D, Blackman M. Infection of dendritic cells by a gamma2-herpesvirus induces functional modulation. J Immunol. 2005;175:3225-34 pubmed
product information
Product Type :
Antibody
Product Name :
IL-10 Monoclonal Antibody (JES5-2A5), Functional Grade, eBioscience
Catalog # :
16-7102-025
Quantity :
25 mg
Price :
US 8250
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Functional Assay: Assay-Dependent, Neutralization: Assay-Dependent
Species :
Mouse
Clone :
JES5-2A5
Isotype :
IgG1, kappa
Storage :
4° C
Description :
Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.
Format :
Liquid
Applications w/Dilutions :
Functional Assay: Assay-Dependent, Neutralization: Assay-Dependent
Aliases :
CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA