This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
IL-10 Monoclonal Antibody (JES5-16E3), Functional Grade, eBioscience
catalog :
16-7101-025
quantity :
25 mg
price :
US 8250
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
JES5-16E3
reactivity :
mouse
application :
neutralization, flow cytometry
citations: 34
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s3, 4b
Keller E, Dvorina N, Jørgensen T. Spontaneous CD4+ T Cell Activation and Differentiation in Lupus-Prone B6.Nba2 Mice Is IFNAR-Independent. Int J Mol Sci. 2022;23: pubmed publisher
Ma S, Patel S, Abe Y, Chen N, Patel P, Cho B, et al. RORγt phosphorylation protects against T cell-mediated inflammation. Cell Rep. 2022;38:110520 pubmed publisher
Medina Buelvas D, Rodr xed guez Sosa M, Vega L. Characterisation of Macrophage Polarisation in Mice Infected with Ninoa Strain of Trypanosoma cruzi. Pathogens. 2021;10: pubmed publisher
Lin C, Chen M, Lin L, Christian D, Min B, Hunter C, et al. Gut epithelial IL-27 confers intestinal immunity through the induction of intraepithelial lymphocytes. J Exp Med. 2021;218: pubmed publisher
Tong L, Hao H, Zhang Z, Lv Y, Liang X, Liu Q, et al. Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota. Theranostics. 2021;11:8570-8586 pubmed publisher
Chen J, Ding Y, Huang F, Lan R, Wang Z, Huang W, et al. Irradiated whole-cell vaccine suppresses hepatocellular carcinoma growth in mice via Th9 cells. Oncol Lett. 2021;21:409 pubmed publisher
Kim J, Kim S, Park J, Hwang S, Choi J, Jung K, et al. Metformin improves salivary gland inflammation and hypofunction in murine Sjögren's syndrome. Arthritis Res Ther. 2019;21:136 pubmed publisher
Crosby F, Lundgren A, Hoffman C, Pascual D, Barbet A. VirB10 vaccination for protection against Anaplasma phagocytophilum. BMC Microbiol. 2018;18:217 pubmed publisher
Pan W, Xu H, Hao W, Sun F, Qin Y, Hao S, et al. The excretory-secretory products of Echinococcus granulosus protoscoleces stimulated IL-10 production in B cells via TLR-2 signaling. BMC Immunol. 2018;19:29 pubmed publisher
Huang S, Wei P, Hwang Verslues W, Kuo W, Jeng Y, Hu C, et al. TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB. EMBO Mol Med. 2017;9:1660-1680 pubmed publisher
Ni G, Liao Z, Chen S, Wang T, Yuan J, Pan X, et al. Blocking IL-10 signalling at the time of immunization does not increase unwanted side effects in mice. BMC Immunol. 2017;18:40 pubmed publisher
Barrett J, Henry R, Villapol S, Stoica B, Kumar A, Burns M, et al. NOX2 deficiency alters macrophage phenotype through an IL-10/STAT3 dependent mechanism: implications for traumatic brain injury. J Neuroinflammation. 2017;14:65 pubmed publisher
Cruz L, Hashemifar S, Wu C, Cho S, Nguyen D, Lin L, et al. Excessive expression of miR-27 impairs Treg-mediated immunological tolerance. J Clin Invest. 2017;127:530-542 pubmed publisher
Alrefai H, Muhammad K, Rudolf R, Pham D, Klein Hessling S, Patra A, et al. NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells. Nat Commun. 2016;7:11724 pubmed publisher
Tian Y, Kuo C, Akbari O, Ou J. Maternal-Derived Hepatitis B Virus e Antigen Alters Macrophage Function in Offspring to Drive Viral Persistence after Vertical Transmission. Immunity. 2016;44:1204-14 pubmed publisher
Singh S, Vedi S, Samrat S, Li W, Kumar R, Agrawal B. Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines. PLoS ONE. 2016;11:e0146404 pubmed publisher
Roberts R, Eitas T, Byrne J, Johnson B, Short P, McKinnon K, et al. Towards programming immune tolerance through geometric manipulation of phosphatidylserine. Biomaterials. 2015;72:1-10 pubmed publisher
Kratochvill F, Gratz N, Qualls J, Van De Velde L, Chi H, Kovarik P, et al. Tristetraprolin Limits Inflammatory Cytokine Production in Tumor-Associated Macrophages in an mRNA Decay-Independent Manner. Cancer Res. 2015;75:3054-64 pubmed publisher
Song N, Gao L, Qiu H, Huang C, Cheng H, Zhou H, et al. Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant. Int J Mol Med. 2015;36:139-49 pubmed publisher
Suryawanshi A, Manoharan I, Hong Y, Swafford D, Majumdar T, Taketo M, et al. Canonical wnt signaling in dendritic cells regulates Th1/Th17 responses and suppresses autoimmune neuroinflammation. J Immunol. 2015;194:3295-304 pubmed publisher
Kasahara K, Sasaki N, Yamashita T, Kita T, Yodoi K, Sasaki Y, et al. CD3 antibody and IL-2 complex combination therapy inhibits atherosclerosis by augmenting a regulatory immune response. J Am Heart Assoc. 2014;3:e000719 pubmed publisher
Bakhru P, Sirisaengtaksin N, Soudani E, Mukherjee S, Khan A, Jagannath C. BCG vaccine mediated reduction in the MHC-II expression of macrophages and dendritic cells is reversed by activation of Toll-like receptors 7 and 9. Cell Immunol. 2014;287:53-61 pubmed publisher
Wang S, Wu B, Xue J, Wang M, Chen R, Wang B. Nizatidine, a small molecular compound, enhances killed H5N1 vaccine cell-mediated responses and protects mice from lethal viral challenge. Hum Vaccin Immunother. 2014;10:461-8 pubmed publisher
Obregón Henao A, Henao Tamayo M, Orme I, Ordway D. Gr1(int)CD11b+ myeloid-derived suppressor cells in Mycobacterium tuberculosis infection. PLoS ONE. 2013;8:e80669 pubmed publisher
Kalli F, Machiorlatti R, Battaglia F, Parodi A, Conteduca G, Ferrera F, et al. Comparative analysis of cancer vaccine settings for the selection of an effective protocol in mice. J Transl Med. 2013;11:120 pubmed publisher
Barron L, Smith A, El Kasmi K, Qualls J, Huang X, Cheever A, et al. Role of arginase 1 from myeloid cells in th2-dominated lung inflammation. PLoS ONE. 2013;8:e61961 pubmed publisher
Wan W, Lionakis M, Liu Q, Roffe E, Murphy P. Genetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice. Cardiovasc Res. 2013;97:580-8 pubmed publisher
Manta C, Heupel E, Radulovic K, Rossini V, Garbi N, Riedel C, et al. CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium. Mucosal Immunol. 2013;6:177-88 pubmed publisher
Ramon H, Beal A, Liu Y, Worthen G, Oliver P. The E3 ubiquitin ligase adaptor Ndfip1 regulates Th17 differentiation by limiting the production of proinflammatory cytokines. J Immunol. 2012;188:4023-31 pubmed publisher
Kar U, Srivastava M, Andersson A, Baratelli F, Huang M, Kickhoefer V, et al. Novel CCL21-vault nanocapsule intratumoral delivery inhibits lung cancer growth. PLoS ONE. 2011;6:e18758 pubmed publisher
COGHILL J, Carlson M, Panoskaltsis Mortari A, West M, Burgents J, Blazar B, et al. Separation of graft-versus-host disease from graft-versus-leukemia responses by targeting CC-chemokine receptor 7 on donor T cells. Blood. 2010;115:4914-22 pubmed publisher
Nijnik A, Madera L, Ma S, Waldbrook M, Elliott M, Easton D, et al. Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment. J Immunol. 2010;184:2539-50 pubmed publisher
Arakaki R, Nagaoka A, Ishimaru N, Yamada A, Yoshida S, Hayashi Y. Role of plasmacytoid dendritic cells for aberrant class II expression in exocrine glands from estrogen-deficient mice of healthy background. Am J Pathol. 2009;174:1715-24 pubmed publisher
Singh A, Carson W, Secor E, Guernsey L, Flavell R, Clark R, et al. Regulatory role of B cells in a murine model of allergic airway disease. J Immunol. 2008;180:7318-26 pubmed
product information
Product Type :
Antibody
Product Name :
IL-10 Monoclonal Antibody (JES5-16E3), Functional Grade, eBioscience
Catalog # :
16-7101-025
Quantity :
25 mg
Price :
US 8250
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
ELISA: 1-4 µg/mL, Functional Assay: Assay-Dependent, Neutralization: Assay-Dependent
Species :
Mouse
Clone :
JES5-16E3
Isotype :
IgG2b, kappa
Storage :
4° C
Description :
Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.
Format :
Liquid
Applications w/Dilutions :
ELISA: 1-4 µg/mL, Functional Assay: Assay-Dependent, Neutralization: Assay-Dependent
Aliases :
CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA