product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
IL-6 Monoclonal Antibody (MP5-20F3), Functional Grade, eBioscience
catalog :
16-7061-38
quantity :
5 mg
price :
US 2175.00
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
MP5-20F3
reactivity :
mouse
application :
neutralization, flow cytometry
more info or order :
citations: 49
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 4
Kim K, Lee J, Ahn S, Won R, Kim S, Jeong S, et al. The methanol extract of Guettarda speciosa Linn. Ameliorates acute lung injury in mice. BMC Complement Med Ther. 2020;20:40 pubmed publisher
  • ELISA; mouse; fig 5
Smith K, Filbey K, Reynolds L, Hewitson J, Harcus Y, Boon L, et al. Low-level regulatory T-cell activity is essential for functional type-2 effector immunity to expel gastrointestinal helminths. Mucosal Immunol. 2016;9:428-43 pubmed publisher
  • flow cytometry; mouse; fig 5
  • ELISA; mouse
Pannu J, Belle J, Forster M, Duerr C, Shen S, Kane L, et al. Ubiquitin specific protease 21 is dispensable for normal development, hematopoiesis and lymphocyte differentiation. PLoS ONE. 2015;10:e0117304 pubmed publisher
  • flow cytometry; mouse; fig 2
Zhang Y, Wu B, Metelli A, Thaxton J, Hong F, Rachidi S, et al. GP96 is a GARP chaperone and controls regulatory T cell functions. J Clin Invest. 2015;125:859-69 pubmed publisher
  • flow cytometry; mouse; fig 1
Mehta P, Nuotio Antar A, Smith C. γδ T cells promote inflammation and insulin resistance during high fat diet-induced obesity in mice. J Leukoc Biol. 2015;97:121-34 pubmed publisher
  • flow cytometry; mouse; fig 1
Lu M, Varley A, Munford R. Persistently active microbial molecules prolong innate immune tolerance in vivo. PLoS Pathog. 2013;9:e1003339 pubmed publisher
  • ELISA; mouse; 2 ug/ml
Hemmers S, Teijaro J, Arandjelovic S, Mowen K. PAD4-mediated neutrophil extracellular trap formation is not required for immunity against influenza infection. PLoS ONE. 2011;6:e22043 pubmed publisher
  • ELISA; mouse; fig 7b
Nijnik A, Madera L, Ma S, Waldbrook M, Elliott M, Easton D, et al. Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment. J Immunol. 2010;184:2539-50 pubmed publisher
  • flow cytometry; mouse; fig 4A
Nakae S, Iwakura Y, Suto H, Galli S. Phenotypic differences between Th1 and Th17 cells and negative regulation of Th1 cell differentiation by IL-17. J Leukoc Biol. 2007;81:1258-68 pubmed
Li D, Huang L, Kan R, Meng X, Wang S, Zou H, et al. LILRB2/PirB mediates macrophage recruitment in fibrogenesis of nonalcoholic steatohepatitis. Nat Commun. 2023;14:4436 pubmed publisher
Long F, Bhatti M, Kellenberger A, Sun W, Modica S, H xf6 ring M, et al. A low-carbohydrate diet induces hepatic insulin resistance and metabolic associated fatty liver disease in mice. Mol Metab. 2023;69:101675 pubmed publisher
Cheng N, Kim K, Lau L. Senescent hepatic stellate cells promote liver regeneration through IL-6 and ligands of CXCR2. JCI Insight. 2022;7: pubmed publisher
Chen D, Jia G, Zhang Y, Mao H, Zhao L, Li W, et al. Sox2 overexpression alleviates noise-induced hearing loss by inhibiting inflammation-related hair cell apoptosis. J Neuroinflammation. 2022;19:59 pubmed publisher
Qi M, Xia Y, Wu Y, Zhang Z, Wang X, Lu L, et al. Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression. Nat Commun. 2022;13:897 pubmed publisher
Luo Y, Guo J, Jia W, Wu M, Yin F, Niu G, et al. TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization. Oxid Med Cell Longev. 2021;2021:3877617 pubmed publisher
Lee G, Liao T, Wu J, Wu K, Kuo C. Restoration of 5-methoxytryptophan protects against atherosclerotic chondrogenesis and calcification in ApoE-/- mice fed high fat diet. J Biomed Sci. 2021;28:74 pubmed publisher
Zhou Z, Choi J, Shin J, Kim D, Kweon B, Oh H, et al. Betulinic Acid Ameliorates the Severity of Acute Pancreatitis via Inhibition of the NF-κB Signaling Pathway in Mice. Int J Mol Sci. 2021;22: pubmed publisher
Rupert J, Narasimhan A, Jengelley D, Jiang Y, Liu J, Au E, et al. Tumor-derived IL-6 and trans-signaling among tumor, fat, and muscle mediate pancreatic cancer cachexia. J Exp Med. 2021;218: pubmed publisher
Alford M, Choi K, Trimble M, Masoudi H, Kalsi P, Pletzer D, et al. Murine Model of Sinusitis Infection for Screening Antimicrobial and Immunomodulatory Therapies. Front Cell Infect Microbiol. 2021;11:621081 pubmed publisher
Ghergurovich J, García Cañaveras J, Wang J, Schmidt E, Zhang Z, Teslaa T, et al. A small molecule G6PD inhibitor reveals immune dependence on pentose phosphate pathway. Nat Chem Biol. 2020;16:731-739 pubmed publisher
Bottek J, Soun C, Lill J, Dixit A, Thiebes S, Beerlage A, et al. Spatial proteomics revealed a CX3CL1-dependent crosstalk between the urothelium and relocated macrophages through IL-6 during an acute bacterial infection in the urinary bladder. Mucosal Immunol. 2020;13:702-714 pubmed publisher
Yeh C, Wu J, Lee G, Wen H, Lin P, Kuo C. Vanadium Derivative Exposure Promotes Functional Alterations of VSMCs and Consequent Atherosclerosis via ROS/p38/NF-κB-Mediated IL-6 Production. Int J Mol Sci. 2019;20: pubmed publisher
Mielke L, Liao Y, Clemens E, Firth M, Duckworth B, Huang Q, et al. TCF-1 limits the formation of Tc17 cells via repression of the MAF-RORγt axis. J Exp Med. 2019;: pubmed publisher
Zhou L, Chu C, Teng F, Bessman N, Goc J, Santosa E, et al. Innate lymphoid cells support regulatory T cells in the intestine through interleukin-2. Nature. 2019;568:405-409 pubmed publisher
Mora Cartín R, Gutiérrez Jiménez C, Alfaro Alarcón A, Chaves Olarte E, Chacón Díaz C, Barquero Calvo E, et al. Neutrophils Dampen Adaptive Immunity in Brucellosis. Infect Immun. 2019;87: pubmed publisher
Düsedau H, Kleveman J, Figueiredo C, Biswas A, Steffen J, Kliche S, et al. p75NTR regulates brain mononuclear cell function and neuronal structure in Toxoplasma infection-induced neuroinflammation. Glia. 2019;67:193-211 pubmed publisher
Gubernatorova E, Gorshkova E, Namakanova O, Zvartsev R, Hidalgo J, Drutskaya M, et al. Non-redundant Functions of IL-6 Produced by Macrophages and Dendritic Cells in Allergic Airway Inflammation. Front Immunol. 2018;9:2718 pubmed publisher
Liu N, Luo J, Kuang D, Xu S, Duan Y, Xia Y, et al. Lactate inhibits ATP6V0d2 expression in tumor-associated macrophages to promote HIF-2α-mediated tumor progression. J Clin Invest. 2019;129:631-646 pubmed publisher
Rodriguez Cetina Biefer H, Heinbokel T, Uehara H, Camacho V, Minami K, Nian Y, et al. Mast cells regulate CD4+ T-cell differentiation in the absence of antigen presentation. J Allergy Clin Immunol. 2018;142:1894-1908.e7 pubmed publisher
Meier L, Auger J, Engelson B, Cowan H, Breed E, Gonzalez Torres M, et al. CD301b/MGL2+ Mononuclear Phagocytes Orchestrate Autoimmune Cardiac Valve Inflammation and Fibrosis. Circulation. 2018;137:2478-2493 pubmed publisher
Sheng L, Jena P, Hu Y, Liu H, Nagar N, Kalanetra K, et al. Hepatic inflammation caused by dysregulated bile acid synthesis is reversible by butyrate supplementation. J Pathol. 2017;243:431-441 pubmed publisher
Jin W, Shi S, Li Z, Li M, Wood K, Gonzales R, et al. Depletion of microglia exacerbates postischemic inflammation and brain injury. J Cereb Blood Flow Metab. 2017;37:2224-2236 pubmed publisher
Zamarron B, Mergian T, Cho K, Martinez Santibañez G, Luan D, Singer K, et al. Macrophage Proliferation Sustains Adipose Tissue Inflammation in Formerly Obese Mice. Diabetes. 2017;66:392-406 pubmed publisher
Pangrazzi L, Meryk A, Naismith E, Koziel R, Lair J, Krismer M, et al. "Inflamm-aging" influences immune cell survival factors in human bone marrow. Eur J Immunol. 2017;47:481-492 pubmed publisher
Nagashima H, Okuyama Y, Hayashi T, Ishii N, So T. TNFR-Associated Factors 2 and 5 Differentially Regulate the Instructive IL-6 Receptor Signaling Required for Th17 Development. J Immunol. 2016;196:4082-9 pubmed publisher
Nullens S, Staessens M, Peleman C, Plaeke P, Malhotra Kumar S, Francque S, et al. Beneficial Effects of Anti-Interleukin-6 Antibodies on Impaired Gastrointestinal Motility, Inflammation and Increased Colonic Permeability in a Murine Model of Sepsis Are Most Pronounced When Administered in a Preventive Setup. PLoS ONE. 2016;11:e0152914 pubmed publisher
Han Y, Choi J, Uyangaa E, Kim S, Kim J, Kim B, et al. Distinct dictation of Japanese encephalitis virus-induced neuroinflammation and lethality via triggering TLR3 and TLR4 signal pathways. PLoS Pathog. 2014;10:e1004319 pubmed publisher
Samuelson E, Laird R, Papillion A, Tatum A, Princiotta M, Hayes S. Reduced B lymphoid kinase (Blk) expression enhances proinflammatory cytokine production and induces nephrosis in C57BL/6-lpr/lpr mice. PLoS ONE. 2014;9:e92054 pubmed publisher
Macia A, Vaquero M, Gou Fabregas M, Castelblanco E, Valdivielso J, Anerillas C, et al. Sprouty1 induces a senescence-associated secretory phenotype by regulating NF?B activity: implications for tumorigenesis. Cell Death Differ. 2014;21:333-43 pubmed publisher
Namjoshi D, Martin G, Donkin J, Wilkinson A, Stukas S, Fan J, et al. The liver X receptor agonist GW3965 improves recovery from mild repetitive traumatic brain injury in mice partly through apolipoprotein E. PLoS ONE. 2013;8:e53529 pubmed publisher
Yee N, Hamerman J. ?(2) integrins inhibit TLR responses by regulating NF-?B pathway and p38 MAPK activation. Eur J Immunol. 2013;43:779-92 pubmed publisher
Tang G, Zhou H, Xu J, Xu J, Ji M, Wu H, et al. Schistosoma japonicum soluble egg antigens attenuate IFN-?-induced MHC class II expression in RAW 264.7 macrophages. PLoS ONE. 2012;7:e49234 pubmed publisher
Osterbur J, Sprague L, Muccioli M, Pate M, Mansfield K, McGinty J, et al. Adhesion to substrates induces dendritic cell endothelization and decreases immunological response. Immunobiology. 2013;218:64-75 pubmed publisher
Huang G, Wang Y, Shi L, Kanneganti T, Chi H. Signaling by the phosphatase MKP-1 in dendritic cells imprints distinct effector and regulatory T cell fates. Immunity. 2011;35:45-58 pubmed publisher
Lal G, Yin N, Xu J, Lin M, Schroppel S, Ding Y, et al. Distinct inflammatory signals have physiologically divergent effects on epigenetic regulation of Foxp3 expression and Treg function. Am J Transplant. 2011;11:203-14 pubmed publisher
Levy D, Kuo A, Chang Y, Schaefer U, Kitson C, Cheung P, et al. Lysine methylation of the NF-κB subunit RelA by SETD6 couples activity of the histone methyltransferase GLP at chromatin to tonic repression of NF-κB signaling. Nat Immunol. 2011;12:29-36 pubmed publisher
Wu Y, El Shikh M, El Sayed R, Best A, Szakal A, Tew J. IL-6 produced by immune complex-activated follicular dendritic cells promotes germinal center reactions, IgG responses and somatic hypermutation. Int Immunol. 2009;21:745-56 pubmed publisher
Maynard C, Hatton R, Helms W, Oliver J, Stephensen C, Weaver C. Contrasting roles for all-trans retinoic acid in TGF-beta-mediated induction of Foxp3 and Il10 genes in developing regulatory T cells. J Exp Med. 2009;206:343-57 pubmed publisher
Kielar M, John R, Bennett M, Richardson J, Shelton J, Chen L, et al. Maladaptive role of IL-6 in ischemic acute renal failure. J Am Soc Nephrol. 2005;16:3315-25 pubmed
product information
Product Type :
Antibody
Product Name :
IL-6 Monoclonal Antibody (MP5-20F3), Functional Grade, eBioscience
Catalog # :
16-7061-38
Quantity :
5 mg
Price :
US 2175.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Functional Assay: Assay-Dependent, Immunostaining: 0.5-2 µg/mL, Neutralization: Assay-Dependent
Species :
Mouse
Clone :
MP5-20F3
Isotype :
IgG1, kappa
Storage :
4° C
Description :
IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
Format :
Liquid
Applications w/Dilutions :
Functional Assay: Assay-Dependent, Immunostaining: 0.5-2 µg/mL, Neutralization: Assay-Dependent
Aliases :
B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA