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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), Functional Grade, eBioscience
catalog :
16-5999-82
quantity :
100 µg
price :
US 154
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
28-14-8
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 37
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 4a
James K, Cosway E, LUCAS B, White A, Parnell S, Carvalho Gaspar M, et al. Endothelial cells act as gatekeepers for LTβR-dependent thymocyte emigration. J Exp Med. 2018;215:2984-2993 pubmed publisher
  • flow cytometry; mouse; 1:250; fig 3
Ludigs K, Jandus C, Utzschneider D, Staehli F, Bessoles S, Dang A, et al. NLRC5 shields T lymphocytes from NK-cell-mediated elimination under inflammatory conditions. Nat Commun. 2016;7:10554 pubmed publisher
  • flow cytometry; mouse; fig 3
Sun H, Zhang J, Chen F, Chen X, Zhou Z, Wang H. Activation of RAW264.7 macrophages by the polysaccharide from the roots of Actinidia eriantha and its molecular mechanisms. Carbohydr Polym. 2015;121:388-402 pubmed publisher
  • flow cytometry; mouse
Wei H, Nash W, Makrigiannis A, Brown M. Impaired NK-cell education diminishes resistance to murine CMV infection. Eur J Immunol. 2014;44:3273-82 pubmed publisher
  • flow cytometry; mouse; fig 4
Rauen J, Kreer C, Paillard A, van Duikeren S, Benckhuijsen W, Camps M, et al. Enhanced cross-presentation and improved CD8+ T cell responses after mannosylation of synthetic long peptides in mice. PLoS ONE. 2014;9:e103755 pubmed publisher
Hinterbrandner M, Rubino V, Stoll C, Forster S, Schnüriger N, Radpour R, et al. Tnfrsf4-expressing regulatory T cells promote immune escape of chronic myeloid leukemia stem cells. JCI Insight. 2021;6: pubmed publisher
Bekeschus S, Clemen R, Nießner F, Sagwal S, Freund E, Schmidt A. Medical Gas Plasma Jet Technology Targets Murine Melanoma in an Immunogenic Fashion. Adv Sci (Weinh). 2020;7:1903438 pubmed publisher
Westrich J, Vermeer D, Silva A, Bonney S, Berger J, Cicchini L, et al. CXCL14 suppresses human papillomavirus-associated head and neck cancer through antigen-specific CD8+ T-cell responses by upregulating MHC-I expression. Oncogene. 2019;: pubmed publisher
Buxade M, Huerga Encabo H, Riera Borrull M, Quintana Gallardo L, López Cotarelo P, Tellechea M, et al. Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5. J Exp Med. 2018;215:2901-2918 pubmed publisher
Murakami G, Edamura M, Furukawa T, Kawasaki H, Kosugi I, Fukuda A, et al. MHC class I in dopaminergic neurons suppresses relapse to reward seeking. Sci Adv. 2018;4:eaap7388 pubmed publisher
Ahn R, Sabourin V, Bolt A, Hebert S, Totten S, De Jay N, et al. The Shc1 adaptor simultaneously balances Stat1 and Stat3 activity to promote breast cancer immune suppression. Nat Commun. 2017;8:14638 pubmed publisher
Kreer C, Kuepper J, Zehner M, Quast T, Kolanus W, Schumak B, et al. N-glycosylation converts non-glycoproteins into mannose receptor ligands and reveals antigen-specific T cell responses in vivo. Oncotarget. 2017;8:6857-6872 pubmed publisher
Lizotte P, Jones R, Keogh L, Ivanova E, Liu H, Awad M, et al. Fine needle aspirate flow cytometric phenotyping characterizes immunosuppressive nature of the mesothelioma microenvironment. Sci Rep. 2016;6:31745 pubmed publisher
Yu C, Burns J, Robinson W, Utz P, Ho P, Steinman L, et al. Identification of Candidate Tolerogenic CD8(+) T Cell Epitopes for Therapy of Type 1 Diabetes in the NOD Mouse Model. J Diabetes Res. 2016;2016:9083103 pubmed publisher
Rota G, Ludigs K, Siegert S, Tardivel A, Morgado L, Reith W, et al. T Cell Priming by Activated Nlrc5-Deficient Dendritic Cells Is Unaffected despite Partially Reduced MHC Class I Levels. J Immunol. 2016;196:2939-46 pubmed publisher
Geherin S, Gómez D, Glabman R, Ruthel G, Hamann A, Debes G. IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require α4β1 Integrin To Migrate between the Peritoneum and Inflamed Skin. J Immunol. 2016;196:2514-2525 pubmed publisher
Plaks V, Boldajipour B, Linnemann J, Nguyen N, Kersten K, Wolf Y, et al. Adaptive Immune Regulation of Mammary Postnatal Organogenesis. Dev Cell. 2015;34:493-504 pubmed publisher
Vom Berg J, Vrohlings M, Haller S, Haimovici A, Kulig P, Sledzińska A, et al. Intratumoral IL-12 combined with CTLA-4 blockade elicits T cell-mediated glioma rejection. J Exp Med. 2013;210:2803-11 pubmed publisher
Qin Y, Zhou P, Zhou C, Li J, Gao W. The adipose-derived lineage-negative cells are enriched mesenchymal stem cells and promote limb ischemia recovery in mice. Stem Cells Dev. 2014;23:363-71 pubmed publisher
Brentville V, Metheringham R, Gunn B, Durrant L. High avidity cytotoxic T lymphocytes can be selected into the memory pool but they are exquisitely sensitive to functional impairment. PLoS ONE. 2012;7:e41112 pubmed publisher
Cho H, Reyes Vargas E, Delgado J, Celis E. A potent vaccination strategy that circumvents lymphodepletion for effective antitumor adoptive T-cell therapy. Cancer Res. 2012;72:1986-95 pubmed publisher
Nouri Shirazi M, Guinet E. Exposure to nicotine adversely affects the dendritic cell system and compromises host response to vaccination. J Immunol. 2012;188:2359-70 pubmed publisher
Kurotaki D, Kon S, Bae K, Ito K, Matsui Y, Nakayama Y, et al. CSF-1-dependent red pulp macrophages regulate CD4 T cell responses. J Immunol. 2011;186:2229-37 pubmed publisher
Nice T, Deng W, Coscoy L, Raulet D. Stress-regulated targeting of the NKG2D ligand Mult1 by a membrane-associated RING-CH family E3 ligase. J Immunol. 2010;185:5369-76 pubmed publisher
van den Boorn J, Konijnenberg D, Tjin E, Picavet D, Meeuwenoord N, Filippov D, et al. Effective melanoma immunotherapy in mice by the skin-depigmenting agent monobenzone and the adjuvants imiquimod and CpG. PLoS ONE. 2010;5:e10626 pubmed publisher
Eweda G, Suzuki D, Nagata T, Tsujimura K, Koide Y. Identification of murine T-cell epitopes on low-molecular-mass secretory proteins (CFP11, CFP17, and TB18.5) of Mycobacterium tuberculosis. Vaccine. 2010;28:4616-25 pubmed publisher
Suzuki D, Nagata T, Eweda G, Matsumoto S, Matsumoto M, Tsujimura K, et al. Characterization of murine T-cell epitopes on mycobacterial DNA-binding protein 1 (MDP1) using DNA vaccination. Vaccine. 2010;28:2020-5 pubmed publisher
Zeng M, Guinet E, Nouri Shirazi M. Comparative analysis of dendritic cells and anti-CD3/CD28 expanded regulatory T cells for application in transplantation. Transpl Immunol. 2009;22:82-92 pubmed publisher
Phares T, Ramakrishna C, Parra G, Epstein A, Chen L, Atkinson R, et al. Target-dependent B7-H1 regulation contributes to clearance of central nervous system infection and dampens morbidity. J Immunol. 2009;182:5430-8 pubmed publisher
Panchal R, Ulrich R, Bradfute S, Lane D, Ruthel G, Kenny T, et al. Reduced expression of CD45 protein-tyrosine phosphatase provides protection against anthrax pathogenesis. J Biol Chem. 2009;284:12874-85 pubmed publisher
Frenzel L, Abdullah Z, Kriegeskorte A, Dieterich R, Lange N, Busch D, et al. Role of natural-killer group 2 member D ligands and intercellular adhesion molecule 1 in natural killer cell-mediated lysis of murine embryonic stem cells and embryonic stem cell-derived cardiomyocytes. Stem Cells. 2009;27:307-16 pubmed publisher
Rifa i M, Shi Z, Zhang S, Lee Y, Shiku H, Isobe K, et al. CD8+CD122+ regulatory T cells recognize activated T cells via conventional MHC class I-alphabetaTCR interaction and become IL-10-producing active regulatory cells. Int Immunol. 2008;20:937-47 pubmed publisher
Malone K, Stohlman S, Ramakrishna C, Macklin W, Bergmann C. Induction of class I antigen processing components in oligodendroglia and microglia during viral encephalomyelitis. Glia. 2008;56:426-35 pubmed publisher
Kuwano Y, Prazma C, Yazawa N, Watanabe R, Ishiura N, Kumanogoh A, et al. CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells. Int Immunol. 2007;19:977-92 pubmed
Xie X, Dighe A, Clark P, Sabastian P, Buss S, Brown M. Deficient major histocompatibility complex-linked innate murine cytomegalovirus immunity in MA/My.L-H2b mice and viral downregulation of H-2k class I proteins. J Virol. 2007;81:229-36 pubmed
Kemball C, Lee E, Vezys V, Pearson T, Larsen C, Lukacher A. Late priming and variability of epitope-specific CD8+ T cell responses during a persistent virus infection. J Immunol. 2005;174:7950-60 pubmed
Blattman J, Antia R, Sourdive D, Wang X, Kaech S, Murali Krishna K, et al. Estimating the precursor frequency of naive antigen-specific CD8 T cells. J Exp Med. 2002;195:657-64 pubmed
product information
Product Type :
Antibody
Product Name :
MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), Functional Grade, eBioscience
Catalog # :
16-5999-82
Quantity :
100 µg
Price :
US 154
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Mouse
Applications :
Control: Assay-Dependent, Flow Cytometry: 0.5 µg/test, Functional Assay: Assay-Dependent
Species :
Mouse
Clone :
28-14-8
Isotype :
IgG2a, kappa
Storage :
4° C
Description :
This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
Format :
Liquid
Applications w/Dilutions :
Control: Assay-Dependent, Flow Cytometry: 0.5 µg/test, Functional Assay: Assay-Dependent
Aliases :
H-2 class I histocompatibility antigen, D-B alpha chain; H-2D; H2-D; H-2D cell surface glycoprotein; H-2D(B); H2D1; H2-D1; H2DB; H2-DC1-beta transplantation antigen protein; H2L; H2LD; histocompatibility 2, D region locus 1; MHC class I antigen; MHC class I H2 antigen; MHC class I H-2Dp; MHC H2-D-q alpha-chain; Q5k protein
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA