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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Functional Grade, eBioscience
catalog :
16-5321-050
quantity :
50 mg
price :
US 12850
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
M5/114.15.2
reactivity :
mouse
application :
neutralization, flow cytometry, immunohistochemistry - paraffin section
more info or order :
citations: 97
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s1
Hoffman R, Huang S, Chalasani G, Vallejo A. Disparate Recruitment and Retention of Plasmacytoid Dendritic Cells to The Small Intestinal Mucosa between Young and Aged Mice. Aging Dis. 2021;12:1183-1196 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4b
Petermann M, Orfanos Z, Sellau J, Gharaibeh M, Lotter H, Fleischer B, et al. CCR2 Deficiency Impairs Ly6Clo and Ly6Chi Monocyte Responses in Orientia tsutsugamushi Infection. Front Immunol. 2021;12:670219 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1d, 3f
Oikonomou N, Schuijs M, Chatzigiagkos A, Androulidaki A, Aidinis V, Hammad H, et al. Airway epithelial cell necroptosis contributes to asthma exacerbation in a mouse model of house dust mite-induced allergic inflammation. Mucosal Immunol. 2021;14:1160-1171 pubmed publisher
  • flow cytometry; mouse; loading ...
McElrath C, Espinosa V, Lin J, Peng J, Sridhar R, Dutta O, et al. Critical role of interferons in gastrointestinal injury repair. Nat Commun. 2021;12:2624 pubmed publisher
  • flow cytometry; mouse
Chu A, Kok S, TSUI J, Lin M, Aguirre B, Wadehra M. Epithelial membrane protein 2 (Emp2) modulates innate immune cell population recruitment at the maternal-fetal interface. J Reprod Immunol. 2021;145:103309 pubmed publisher
  • flow cytometry; mouse; fig 4a
Zhao L, Hu S, Davila M, Yang J, Lin Y, Albanese J, et al. Coordinated co-migration of CCR10+ antibody-producing B cells with helper T cells for colonic homeostatic regulation. Mucosal Immunol. 2021;14:420-430 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...
Jensen I, Jensen S, Sjaastad F, Gibson Corley K, Dileepan T, Griffith T, et al. Sepsis impedes EAE disease development and diminishes autoantigen-specific naive CD4 T cells. elife. 2020;9: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 4a
Williams G, Marmion D, Schonhoff A, Jurkuvenaite A, Won W, Standaert D, et al. T cell infiltration in both human multiple system atrophy and a novel mouse model of the disease. Acta Neuropathol. 2020;139:855-874 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e10
Majer O, Liu B, Kreuk L, Krogan N, Barton G. UNC93B1 recruits syntenin-1 to dampen TLR7 signalling and prevent autoimmunity. Nature. 2019;575:366-370 pubmed publisher
Budhu S, Giese R, Gupta A, Fitzgerald K, Zappasodi R, Schad S, et al. Targeting Phosphatidylserine Enhances the Anti-tumor Response to Tumor-Directed Radiation Therapy in a Preclinical Model of Melanoma. Cell Rep. 2021;34:108620 pubmed publisher
Huang L, Fang X, Zhang X, Wu W, Yao H, Fang Q. RON Expression Mediates Lipopolysaccharide-Mediated Dendritic Cell Maturation via March-I. Front Cell Infect Microbiol. 2020;10:606340 pubmed publisher
Kadir N, Acosta A, Sarmiento M, Norazmi M. Immunomodulatory Effects of Recombinant Mycobacterium smegmatis Expressing Antigen-85B Epitopes in Infected J774A.1 Murine Macrophages. Pathogens. 2020;9: pubmed publisher
Lu S, Jolly A, Strand K, Dubner A, Mutryn M, Moulton K, et al. Smooth muscle-derived progenitor cell myofibroblast differentiation through KLF4 downregulation promotes arterial remodeling and fibrosis. JCI Insight. 2020;5: pubmed publisher
Menga A, Serra M, Todisco S, Riera Domingo C, Ammarah U, Ehling M, et al. Glufosinate constrains synchronous and metachronous metastasis by promoting anti-tumor macrophages. EMBO Mol Med. 2020;12:e11210 pubmed publisher
Ko H, Hong S, Verma R, Jung J, Lee M, Kim N, et al. Dietary Glucose Consumption Promotes RALDH Activity in Small Intestinal CD103+CD11b+ Dendritic Cells. Front Immunol. 2020;11:1897 pubmed publisher
Campesato L, Budhu S, Tchaicha J, Weng C, Gigoux M, Cohen I, et al. Blockade of the AHR restricts a Treg-macrophage suppressive axis induced by L-Kynurenine. Nat Commun. 2020;11:4011 pubmed publisher
Aires I, Ribeiro Rodrigues T, Boia R, Catarino S, Girao H, Ambrósio A, et al. Exosomes derived from microglia exposed to elevated pressure amplify the neuroinflammatory response in retinal cells. Glia. 2020;68:2705-2724 pubmed publisher
Zhu X, Liu H, Zhang Z, Wei R, Zhou X, Wang Z, et al. MiR-103 protects from recurrent spontaneous abortion via inhibiting STAT1 mediated M1 macrophage polarization. Int J Biol Sci. 2020;16:2248-2264 pubmed publisher
LUCAS B, White A, Cosway E, Parnell S, James K, Jones N, et al. Diversity in medullary thymic epithelial cells controls the activity and availability of iNKT cells. Nat Commun. 2020;11:2198 pubmed publisher
Alkhani A, Levy C, Tsui M, Rosenberg K, Polovina K, Mattis A, et al. Ly6cLo non-classical monocytes promote resolution of rhesus rotavirus-mediated perinatal hepatic inflammation. Sci Rep. 2020;10:7165 pubmed publisher
Neuwelt A, Kimball A, Johnson A, Arnold B, Bullock B, Kaspar R, et al. Cancer cell-intrinsic expression of MHC II in lung cancer cell lines is actively restricted by MEK/ERK signaling and epigenetic mechanisms. J Immunother Cancer. 2020;8: pubmed publisher
Hu X, Deng Q, Ma L, Li Q, Chen Y, Liao Y, et al. Meningeal lymphatic vessels regulate brain tumor drainage and immunity. Cell Res. 2020;30:229-243 pubmed publisher
Wang L, Li Y, Wang X, Wang P, Essandoh K, Cui S, et al. GDF3 Protects Mice against Sepsis-Induced Cardiac Dysfunction and Mortality by Suppression of Macrophage Pro-Inflammatory Phenotype. Cells. 2020;9: pubmed publisher
Dokoshi T, Zhang L, Li F, Nakatsuji T, Butcher A, Yoshida H, et al. Hyaluronan Degradation by Cemip Regulates Host Defense against Staphylococcus aureus Skin Infection. Cell Rep. 2020;30:61-68.e4 pubmed publisher
Fang D, Tang Q, Kong Y, Wang Q, Gu J, Fang X, et al. MDM2 inhibitor APG-115 synergizes with PD-1 blockade through enhancing antitumor immunity in the tumor microenvironment. J Immunother Cancer. 2019;7:327 pubmed publisher
Aires I, Madeira M, Boia R, Rodrigues Neves A, Martins J, Ambrósio A, et al. Intravitreal injection of adenosine A2A receptor antagonist reduces neuroinflammation, vascular leakage and cell death in the retina of diabetic mice. Sci Rep. 2019;9:17207 pubmed publisher
Wang Z, Chen J, Hu J, Zhang H, Xu F, He W, et al. cGAS/STING axis mediates a topoisomerase II inhibitor-induced tumor immunogenicity. J Clin Invest. 2019;129:4850-4862 pubmed publisher
Sheng W, Chauhan P, Hu S, Prasad S, Lokensgard J. Antiallodynic Effects of Cannabinoid Receptor 2 (CB2R) Agonists on Retrovirus Infection-Induced Neuropathic Pain. Pain Res Manag. 2019;2019:1260353 pubmed publisher
Lin J, Wang Z, Wang J, Yang Q. Microarray analysis of infectious bronchitis virus infection of chicken primary dendritic cells. BMC Genomics. 2019;20:557 pubmed publisher
Pascual García M, Bonfill Teixidor E, Planas Rigol E, Rubio Perez C, Iurlaro R, Arias A, et al. LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8+ T cell tumor-infiltration impairing anti-PD1 therapy. Nat Commun. 2019;10:2416 pubmed publisher
Flores C, Wildes T, Drake J, Moore G, Dean B, Abraham R, et al. Lin-CCR2+ hematopoietic stem and progenitor cells overcome resistance to PD-1 blockade. Nat Commun. 2018;9:4313 pubmed publisher
Buxade M, Huerga Encabo H, Riera Borrull M, Quintana Gallardo L, López Cotarelo P, Tellechea M, et al. Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5. J Exp Med. 2018;215:2901-2918 pubmed publisher
Klein B, Mrowetz H, Barker C, Lange S, Rivera F, Aigner L. Age Influences Microglial Activation After Cuprizone-Induced Demyelination. Front Aging Neurosci. 2018;10:278 pubmed publisher
Bonacina F, Coe D, Wang G, Longhi M, Baragetti A, Moregola A, et al. Myeloid apolipoprotein E controls dendritic cell antigen presentation and T cell activation. Nat Commun. 2018;9:3083 pubmed publisher
García Nores G, Ly C, Cuzzone D, Kataru R, Hespe G, Torrisi J, et al. CD4+ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema. Nat Commun. 2018;9:1970 pubmed publisher
Huang C, Zhang L, Ling F, Wen S, Luo Y, Liu H, et al. Effect of immune tolerance induced by immature dendritic cells and CTLA4-Ig on systemic lupus erythematosus: An in vivo study. Exp Ther Med. 2018;15:2499-2506 pubmed publisher
Bai W, Li C, Zhang C, Xu L, Zeng Q, Hu B, et al. Prolonged Deleterious Influences of Chemotherapeutic Agent CPT-11 on Resident Peritoneal Macrophages and B1 Cells. Front Immunol. 2017;8:1919 pubmed publisher
Geslewitz W, Percopo C, Rosenberg H. FACS isolation of live mouse eosinophils at high purity via a protocol that does not target Siglec F. J Immunol Methods. 2018;454:27-31 pubmed publisher
Gaya M, Barral P, Burbage M, Aggarwal S, Montaner B, Warren Navia A, et al. Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells. Cell. 2018;172:517-533.e20 pubmed publisher
Kim S, Bielawski J, Yang H, Kong Y, Zhou B, Li J. Functional antagonism of sphingosine-1-phosphate receptor 1 prevents cuprizone-induced demyelination. Glia. 2017;: pubmed publisher
Harms A, Delic V, Thome A, Bryant N, Liu Z, Chandra S, et al. α-Synuclein fibrils recruit peripheral immune cells in the rat brain prior to neurodegeneration. Acta Neuropathol Commun. 2017;5:85 pubmed publisher
Martin T, Möglich A, Felix I, Förtsch C, Rittlinger A, Palmer A, et al. Rho-inhibiting C2IN-C3 fusion toxin inhibits chemotactic recruitment of human monocytes ex vivo and in mice in vivo. Arch Toxicol. 2018;92:323-336 pubmed publisher
Wang J, Dai X, Hsu C, Ming C, He Y, Zhang J, et al. Discrimination of the heterogeneity of bone marrow‑derived dendritic cells. Mol Med Rep. 2017;16:6787-6793 pubmed publisher
Cruz S, Hari A, Qin Z, Couture P, Huang H, Lagace D, et al. Loss of IRF2BP2 in Microglia Increases Inflammation and Functional Deficits after Focal Ischemic Brain Injury. Front Cell Neurosci. 2017;11:201 pubmed publisher
McDonald L, Johnson S, Russell D, Young M, LaRue A. Role of a novel immune modulating DDR2-expressing population in silica-induced pulmonary fibrosis. PLoS ONE. 2017;12:e0180724 pubmed publisher
Nouri Shirazi M, Bible B, Zeng M, Tamjidi S, Bossart G. Phenotyping and comparing the immune cell populations of free-ranging Atlantic bottlenose dolphins (Tursiops truncatus) and dolphins under human care. BMC Vet Res. 2017;13:78 pubmed publisher
Macpherson K, Sompol P, Kannarkat G, Chang J, Sniffen L, Wildner M, et al. Peripheral administration of the soluble TNF inhibitor XPro1595 modifies brain immune cell profiles, decreases beta-amyloid plaque load, and rescues impaired long-term potentiation in 5xFAD mice. Neurobiol Dis. 2017;102:81-95 pubmed publisher
Ogawa Y, He H, Mukai S, Imada T, Nakamura S, Su C, et al. Heavy Chain-Hyaluronan/Pentraxin 3 from Amniotic Membrane Suppresses Inflammation and Scarring in Murine Lacrimal Gland and Conjunctiva of Chronic Graft-versus-Host Disease. Sci Rep. 2017;7:42195 pubmed publisher
Berlato C, Khan M, Schioppa T, Thompson R, Maniati E, Montfort A, et al. A CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer. J Clin Invest. 2017;127:801-813 pubmed publisher
Wang H, Hu W, Liang Z, Zeng L, Li J, Yan H, et al. Thiol peroxiredoxin, a novel allergen from Bombyx mori, modulates functions of macrophages and dendritic cells. Am J Transl Res. 2016;8:5320-5329 pubmed
Le Blon D, Guglielmetti C, Hoornaert C, Quarta A, Daans J, Dooley D, et al. Intracerebral transplantation of interleukin 13-producing mesenchymal stem cells limits microgliosis, oligodendrocyte loss and demyelination in the cuprizone mouse model. J Neuroinflammation. 2016;13:288 pubmed
Guglielmetti C, Le Blon D, Santermans E, Salas Perdomo A, Daans J, De Vocht N, et al. Interleukin-13 immune gene therapy prevents CNS inflammation and demyelination via alternative activation of microglia and macrophages. Glia. 2016;64:2181-2200 pubmed publisher
Klein B, Mrowetz H, Thalhamer J, Scheiblhofer S, Weiss R, Aigner L. Allergy Enhances Neurogenesis and Modulates Microglial Activation in the Hippocampus. Front Cell Neurosci. 2016;10:169 pubmed publisher
Raguz J, Jerić I, Niault T, Nowacka J, Kuzet S, Rupp C, et al. Epidermal RAF prevents allergic skin disease. elife. 2016;5: pubmed publisher
McCullough L, Mirza M, Xu Y, Bentivegna K, Steffens E, Ritzel R, et al. Stroke sensitivity in the aged: sex chromosome complement vs. gonadal hormones. Aging (Albany NY). 2016;8:1432-41 pubmed publisher
Menendez C, Jinkins J, Carr D. Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency. J Immunol. 2016;197:1262-75 pubmed publisher
Zheng H, Zou W, Shen J, Xu L, Wang S, Fu Y, et al. Opposite Effects of Coinjection and Distant Injection of Mesenchymal Stem Cells on Breast Tumor Cell Growth. Stem Cells Transl Med. 2016;5:1216-28 pubmed publisher
Lopes Pinheiro M, Kamermans A, Garcia Vallejo J, Van het Hof B, Wierts L, O Toole T, et al. Internalization and presentation of myelin antigens by the brain endothelium guides antigen-specific T cell migration. elife. 2016;5: pubmed publisher
Xu Y, Liu Y, Yang C, Kang L, Wang M, Hu J, et al. Macrophages transfer antigens to dendritic cells by releasing exosomes containing dead-cell-associated antigens partially through a ceramide-dependent pathway to enhance CD4(+) T-cell responses. Immunology. 2016;149:157-71 pubmed publisher
Vinet J, Vainchtein I, Spano C, Giordano C, Bordini D, Curia G, et al. Microglia are less pro-inflammatory than myeloid infiltrates in the hippocampus of mice exposed to status epilepticus. Glia. 2016;64:1350-62 pubmed publisher
Brandstätter O, Schanz O, Vorac J, König J, Mori T, Maruyama T, et al. Balancing intestinal and systemic inflammation through cell type-specific expression of the aryl hydrocarbon receptor repressor. Sci Rep. 2016;6:26091 pubmed publisher
Qin H, Buckley J, Li X, Liu Y, Fox T, Meares G, et al. Inhibition of the JAK/STAT Pathway Protects Against α-Synuclein-Induced Neuroinflammation and Dopaminergic Neurodegeneration. J Neurosci. 2016;36:5144-59 pubmed publisher
Xu X, Gao Y, Zhai Z, Zhang S, Shan F, Feng J. Repulsive guidance molecule a blockade exerts the immunoregulatory function in DCs stimulated with ABP and LPS. Hum Vaccin Immunother. 2016;12:2169-2180 pubmed
Xiao J, Shao L, Shen J, Jiang W, Feng Y, Zheng P, et al. Effects of ketanserin on experimental colitis in mice and macrophage function. Int J Mol Med. 2016;37:659-68 pubmed publisher
Grabert K, Michoel T, Karavolos M, Clohisey S, Baillie J, Stevens M, et al. Microglial brain region-dependent diversity and selective regional sensitivities to aging. Nat Neurosci. 2016;19:504-16 pubmed publisher
Connor A, Kelley P, Tempero R. Lymphatic endothelial lineage assemblage during corneal lymphangiogenesis. Lab Invest. 2016;96:270-82 pubmed publisher
Lagassé H, Anidi I, Craig J, Limjunyawong N, Poupore A, Mitzner W, et al. Recruited monocytes modulate malaria-induced lung injury through CD36-mediated clearance of sequestered infected erythrocytes. J Leukoc Biol. 2016;99:659-71 pubmed publisher
Plaks V, Boldajipour B, Linnemann J, Nguyen N, Kersten K, Wolf Y, et al. Adaptive Immune Regulation of Mammary Postnatal Organogenesis. Dev Cell. 2015;34:493-504 pubmed publisher
DeFalco T, Potter S, Williams A, Waller B, Kan M, Capel B. Macrophages Contribute to the Spermatogonial Niche in the Adult Testis. Cell Rep. 2015;12:1107-19 pubmed publisher
Lee N, Muramatsu S, Chien Y, Liu W, Wang W, Cheng C, et al. Benefits of Neuronal Preferential Systemic Gene Therapy for Neurotransmitter Deficiency. Mol Ther. 2015;23:1572-81 pubmed publisher
Huang L, Kong Y, Wang J, Sun J, Shi Q, Qiu Y. Reducing progression of experimental lupus nephritis via inhibition of the B7/CD28 signaling pathway. Mol Med Rep. 2015;12:4187-4195 pubmed publisher
Kurzejamska E, Johansson J, Jirström K, Prakash V, Ananthaseshan S, Boon L, et al. C/EBPβ expression is an independent predictor of overall survival in breast cancer patients by MHCII/CD4-dependent mechanism of metastasis formation. Oncogenesis. 2014;3:e125 pubmed publisher
Xiao G, Liu I, Cheng C, Chang C, Lee Y, Cheng W, et al. Amniotic fluid stem cells prevent follicle atresia and rescue fertility of mice with premature ovarian failure induced by chemotherapy. PLoS ONE. 2014;9:e106538 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Functional Grade, eBioscience
Catalog # :
16-5321-050
Quantity :
50 mg
Price :
US 12850
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.125 µg/test, Functional assay: Assay-Dependent, Neutralization: Assay-Dependent
Species :
Mouse
Clone :
M5/114.15.2
Isotype :
IgG2b, kappa
Storage :
4° C
Description :
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.125 µg/test, Functional assay: Assay-Dependent, Neutralization: Assay-Dependent
Aliases :
Abeta; AI323765; AI845868; cell surface glycoprotein; E-alpha-f; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; H-2Ab; H2-Ab; H2-Ab1; H-2Ea; H2-Ea; H2-Ea-ps; H2Eb; H-2Eb; H2-Eb1; H2-iabeta; H2-IE-alpha; histocompatibility 2, class II antigen A, beta 1; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; histocompatibility 2, class II antigen E beta; Ia2; Ia-2; Ia3; Ia-3; Ia4; Ia-4; IAb; I-Ab; I-Abeta; I-Ad; I-Aq; I-E alpha MHC class II; I-E beta MHC class II; I-Ealpha; I-Ed; I-Ek; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; MHC class 2; MHC class II antigen A beta; MHC class II antigen E alpha; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC class II protein; MHC H2-IE-beta cell surface glycoprotein; response to metastatic cancers 1; Rmcs1
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA