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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD140b (PDGFRB) Monoclonal Antibody (APB5), Functional Grade, eBioscience
catalog :
16-1402-82
quantity :
100 µg
price :
US 246
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
APB5
reactivity :
mouse
application :
western blot, immunohistochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 75
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; loading ...; fig 1b
Choi S, Bae H, Jeong S, Park I, Cho H, Hong S, et al. YAP/TAZ direct commitment and maturation of lymph node fibroblastic reticular cells. Nat Commun. 2020;11:519 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 7b
Nakaya M, Watari K, Tajima M, Nakaya T, Matsuda S, Ohara H, et al. Cardiac myofibroblast engulfment of dead cells facilitates recovery after myocardial infarction. J Clin Invest. 2017;127:383-401 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2
Patenaude J, Perreault C. Thymic Mesenchymal Cells Have a Distinct Transcriptomic Profile. J Immunol. 2016;196:4760-70 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 3c
Chen X, Wei S, Li J, Zhang Q, Wang Y, Zhao S, et al. Overexpression of Heme Oxygenase-1 Prevents Renal Interstitial Inflammation and Fibrosis Induced by Unilateral Ureter Obstruction. PLoS ONE. 2016;11:e0147084 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; fig 1
Naylor A, McGettrick H, Maynard W, May P, Barone F, Croft A, et al. A differential role for CD248 (Endosialin) in PDGF-mediated skeletal muscle angiogenesis. PLoS ONE. 2014;9:e107146 pubmed publisher
  • immunohistochemistry; mouse; fig 1
  • western blot; mouse; fig 9
Lee D, Shenoy S, Nigatu Y, Plotkin M. Id proteins regulate capillary repair and perivascular cell proliferation following ischemia-reperfusion injury. PLoS ONE. 2014;9:e88417 pubmed publisher
Huang X, Yan L, Kou S, Meng J, Lu Z, Lin C, et al. Generation and characterization of a Myh6-driven Cre knockin mouse line. Transgenic Res. 2021;30:821-835 pubmed publisher
Goss G, Rognoni E, Salameti V, Watt F. Distinct Fibroblast Lineages Give Rise to NG2+ Pericyte Populations in Mouse Skin Development and Repair. Front Cell Dev Biol. 2021;9:675080 pubmed publisher
Delgado A, Maldonado Soto A, Silva Vargas V, Mizrak D, von Kanel T, Tan K, et al. Release of stem cells from quiescence reveals gliogenic domains in the adult mouse brain. Science. 2021;372:1205-1209 pubmed publisher
Ruth J, Pant D, Pan T, Seidel H, Baksh S, Keister B, et al. Cellular dormancy in minimal residual disease following targeted therapy. Breast Cancer Res. 2021;23:63 pubmed publisher
Huang M, Chen M, Qi M, Ye G, Pan J, Shi C, et al. Perivascular cell-derived extracellular vesicles stimulate colorectal cancer revascularization after withdrawal of antiangiogenic drugs. J Extracell Vesicles. 2021;10:e12096 pubmed publisher
Julien A, Kanagalingam A, Martínez Sarrà E, Megret J, Luka M, Ménager M, et al. Direct contribution of skeletal muscle mesenchymal progenitors to bone repair. Nat Commun. 2021;12:2860 pubmed publisher
Díaz Lezama N, Wolf A, Koch S, Pfaller A, Biber J, Guillonneau X, et al. PDGF Receptor Alpha Signaling Is Key for Müller Cell Homeostasis Functions. Int J Mol Sci. 2021;22: pubmed publisher
Lee L, Khakoo A, Chintalgattu V. Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease. FEBS Open Bio. 2021;11:207-225 pubmed publisher
Kirita Y, Wu H, Uchimura K, Wilson P, Humphreys B. Cell profiling of mouse acute kidney injury reveals conserved cellular responses to injury. Proc Natl Acad Sci U S A. 2020;117:15874-15883 pubmed publisher
Bar O, Gelb S, Atamny K, Anzi S, Ben Zvi A. Angiomodulin (IGFBP7) is a cerebral specific angiocrine factor, but is probably not a blood-brain barrier inducer. Fluids Barriers CNS. 2020;17:27 pubmed publisher
Hreha T, Collins C, Daugherty A, Twentyman J, Paluri N, Hunstad D. TGFβ1 orchestrates renal fibrosis following Escherichia coli pyelonephritis. Physiol Rep. 2020;8:e14401 pubmed publisher
Lehmann G, Hanke Gogokhia C, Hu Y, Bareja R, Salfati Z, Ginsberg M, et al. Single-cell profiling reveals an endothelium-mediated immunomodulatory pathway in the eye choroid. J Exp Med. 2020;217: pubmed publisher
Castro Dias M, Coisne C, Baden P, Enzmann G, Garrett L, Becker L, et al. Claudin-12 is not required for blood-brain barrier tight junction function. Fluids Barriers CNS. 2019;16:30 pubmed publisher
Huang X, Feng T, Jiang Z, Meng J, Kou S, Lu Z, et al. Dual lineage tracing identifies intermediate mesenchymal stage for endocardial contribution to fibroblasts, coronary mural cells, and adipocytes. J Biol Chem. 2019;294:8894-8906 pubmed publisher
Camps J, Grosemans H, Gijsbers R, Maes C, Sampaolesi M. Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells. Int J Mol Sci. 2019;20: pubmed publisher
Andersson P, Yang Y, Hosaka K, Zhang Y, Fischer C, Braun H, et al. Molecular mechanisms of IL-33-mediated stromal interactions in cancer metastasis. JCI Insight. 2018;3: pubmed publisher
O Hainmhire E, Wu H, Muto Y, Donnelly E, Machado F, Fan L, et al. A conditionally immortalized Gli1-positive kidney mesenchymal cell line models myofibroblast transition. Am J Physiol Renal Physiol. 2019;316:F63-F75 pubmed publisher
Eilken H, Diéguez Hurtado R, Schmidt I, Nakayama M, Jeong H, Arf H, et al. Pericytes regulate VEGF-induced endothelial sprouting through VEGFR1. Nat Commun. 2017;8:1574 pubmed publisher
Epshtein A, Rachi E, Sakhneny L, Mizrachi S, Baer D, Landsman L. Neonatal pancreatic pericytes support β-cell proliferation. Mol Metab. 2017;6:1330-1338 pubmed publisher
Wang Y, Jin Y, Mäe M, Zhang Y, Ortsäter H, Betsholtz C, et al. Smooth muscle cell recruitment to lymphatic vessels requires PDGFB and impacts vessel size but not identity. Development. 2017;144:3590-3601 pubmed publisher
Naiman N, Fujioka K, Fujino M, Valerius M, Potter S, McMahon A, et al. Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development. Dev Cell. 2017;41:349-365.e3 pubmed publisher
Sarkar D, Singh S. Neonatal hypothyroidism affects testicular glucose homeostasis through increased oxidative stress in prepubertal mice: effects on GLUT3, GLUT8 and Cx43. Andrology. 2017;5:749-762 pubmed publisher
Gautam J, Nirwane A, Yao Y. Laminin differentially regulates the stemness of type I and type II pericytes. Stem Cell Res Ther. 2017;8:28 pubmed publisher
Scully K, Skowronska Krawczyk D, Krawczyk M, Merkurjev D, Taylor H, Livolsi A, et al. Epithelial cell integrin β1 is required for developmental angiogenesis in the pituitary gland. Proc Natl Acad Sci U S A. 2016;113:13408-13413 pubmed
Gautam J, Zhang X, Yao Y. The role of pericytic laminin in blood brain barrier integrity maintenance. Sci Rep. 2016;6:36450 pubmed publisher
He L, Vanlandewijck M, Raschperger E, Andaloussi Mäe M, Jung B, Lebouvier T, et al. Analysis of the brain mural cell transcriptome. Sci Rep. 2016;6:35108 pubmed publisher
Sato Y, Mii A, Hamazaki Y, Fujita H, Nakata H, Masuda K, et al. Heterogeneous fibroblasts underlie age-dependent tertiary lymphoid tissues in the kidney. JCI Insight. 2016;1:e87680 pubmed publisher
Chen Q, Zhang H, Liu Y, Adams S, Eilken H, Stehling M, et al. Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells. Nat Commun. 2016;7:12422 pubmed publisher
Gallini R, Huusko J, Yla Herttuala S, Betsholtz C, Andrae J. Isoform-Specific Modulation of Inflammation Induced by Adenoviral Mediated Delivery of Platelet-Derived Growth Factors in the Adult Mouse Heart. PLoS ONE. 2016;11:e0160930 pubmed publisher
Yang Y, Andersson P, Hosaka K, Zhang Y, Cao R, Iwamoto H, et al. The PDGF-BB-SOX7 axis-modulated IL-33 in pericytes and stromal cells promotes metastasis through tumour-associated macrophages. Nat Commun. 2016;7:11385 pubmed publisher
Yao Y, Norris E, Mason C, Strickland S. Laminin regulates PDGFR?(+) cell stemness and muscle development. Nat Commun. 2016;7:11415 pubmed publisher
Kusumbe A, Ramasamy S, Itkin T, Mäe M, Langen U, Betsholtz C, et al. Age-dependent modulation of vascular niches for haematopoietic stem cells. Nature. 2016;532:380-4 pubmed publisher
Przybyszewska M, Miłoszewska J, Kotlarz A, Swoboda P, Pyśniak K, Szczepek W, et al. Imatinib Inhibits the Renewal and Tumorigenicity of CT-26 Colon Cancer Cells after Cytoreductive Treatment with Doxorubicin. Arch Immunol Ther Exp (Warsz). 2017;65:51-67 pubmed publisher
Quattrocelli M, Swinnen M, Giacomazzi G, Camps J, Barthélemy I, Ceccarelli G, et al. Mesodermal iPSC-derived progenitor cells functionally regenerate cardiac and skeletal muscle. J Clin Invest. 2015;125:4463-82 pubmed publisher
Volz K, Jacobs A, Chen H, Poduri A, McKay A, Riordan D, et al. Pericytes are progenitors for coronary artery smooth muscle. elife. 2015;4: pubmed publisher
Maarouf O, Aravamudhan A, Rangarajan D, Kusaba T, Zhang V, Welborn J, et al. Paracrine Wnt1 Drives Interstitial Fibrosis without Inflammation by Tubulointerstitial Cross-Talk. J Am Soc Nephrol. 2016;27:781-90 pubmed publisher
Yin H, Frontini M, Arpino J, Nong Z, O Neil C, Xu Y, et al. Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases. J Biol Chem. 2015;290:22127-42 pubmed publisher
Reed N, Jo H, Chen C, Tsujino K, Arnold T, Degrado W, et al. The αvβ1 integrin plays a critical in vivo role in tissue fibrosis. Sci Transl Med. 2015;7:288ra79 pubmed publisher
Crouch E, Liu C, Silva Vargas V, Doetsch F. Regional and stage-specific effects of prospectively purified vascular cells on the adult V-SVZ neural stem cell lineage. J Neurosci. 2015;35:4528-39 pubmed publisher
Seo J, Maki T, Maeda M, Miyamoto N, Liang A, Hayakawa K, et al. Oligodendrocyte precursor cells support blood-brain barrier integrity via TGF-? signaling. PLoS ONE. 2014;9:e103174 pubmed publisher
Ben Zvi A, Lacoste B, Kur E, Andreone B, Mayshar Y, Yan H, et al. Mfsd2a is critical for the formation and function of the blood-brain barrier. Nature. 2014;509:507-11 pubmed publisher
Eskilsson A, Tachikawa M, Hosoya K, Blomqvist A. Distribution of microsomal prostaglandin E synthase-1 in the mouse brain. J Comp Neurol. 2014;522:3229-44 pubmed publisher
Yao Y, Chen Z, Norris E, Strickland S. Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity. Nat Commun. 2014;5:3413 pubmed publisher
Son A, Sheleg M, Cooper M, Sun Y, Kleiman N, Zhou R. Formation of persistent hyperplastic primary vitreous in ephrin-A5-/- mice. Invest Ophthalmol Vis Sci. 2014;55:1594-606 pubmed publisher
Osada M, Singh V, Wu K, Sant Angelo D, Pezzano M. Label retention identifies a multipotent mesenchymal stem cell-like population in the postnatal thymus. PLoS ONE. 2013;8:e83024 pubmed publisher
Nakayama A, Nakayama M, Turner C, Höing S, Lepore J, Adams R. Ephrin-B2 controls PDGFR? internalization and signaling. Genes Dev. 2013;27:2576-89 pubmed publisher
Qin Y, Zhou P, Zhou C, Li J, Gao W. The adipose-derived lineage-negative cells are enriched mesenchymal stem cells and promote limb ischemia recovery in mice. Stem Cells Dev. 2014;23:363-71 pubmed publisher
Gaál E, Tammela T, Anisimov A, Marbacher S, Honkanen P, Zarkada G, et al. Comparison of vascular growth factors in the murine brain reveals placenta growth factor as prime candidate for CNS revascularization. Blood. 2013;122:658-65 pubmed publisher
DiRocco D, Kobayashi A, Taketo M, McMahon A, Humphreys B. Wnt4/?-catenin signaling in medullary kidney myofibroblasts. J Am Soc Nephrol. 2013;24:1399-412 pubmed publisher
Mendel T, Clabough E, Kao D, Demidova Rice T, Durham J, Zotter B, et al. Pericytes derived from adipose-derived stem cells protect against retinal vasculopathy. PLoS ONE. 2013;8:e65691 pubmed publisher
Ruan J, Luo M, Wang C, Fan L, Yang S, Cárdenas M, et al. Imatinib disrupts lymphoma angiogenesis by targeting vascular pericytes. Blood. 2013;121:5192-202 pubmed publisher
Joo H, Choi D, Lim J, Park J, Lee S, Song S, et al. ROCK suppression promotes differentiation and expansion of endothelial cells from embryonic stem cell-derived Flk1(+) mesodermal precursor cells. Blood. 2012;120:2733-44 pubmed
Kinugasa Y, Takakura N. Monoclonal antibody selectively recognizing murine but not human CD44. Hybridoma (Larchmt). 2012;31:262-6 pubmed publisher
Hashitani H, Mitsui R, Shimizu Y, Higashi R, Nakamura K. Functional and morphological properties of pericytes in suburothelial venules of the mouse bladder. Br J Pharmacol. 2012;167:1723-36 pubmed publisher
Acharya A, Baek S, Huang G, Eskiocak B, Goetsch S, Sung C, et al. The bHLH transcription factor Tcf21 is required for lineage-specific EMT of cardiac fibroblast progenitors. Development. 2012;139:2139-49 pubmed publisher
Rock J, Barkauskas C, Cronce M, Xue Y, Harris J, Liang J, et al. Multiple stromal populations contribute to pulmonary fibrosis without evidence for epithelial to mesenchymal transition. Proc Natl Acad Sci U S A. 2011;108:E1475-83 pubmed publisher
Lee D, Wolstein J, Pudasaini B, Plotkin M. INK4a deletion results in improved kidney regeneration and decreased capillary rarefaction after ischemia-reperfusion injury. Am J Physiol Renal Physiol. 2012;302:F183-91 pubmed publisher
Fuxe J, Tabruyn S, Colton K, Zaid H, Adams A, Baluk P, et al. Pericyte requirement for anti-leak action of angiopoietin-1 and vascular remodeling in sustained inflammation. Am J Pathol. 2011;178:2897-909 pubmed publisher
Kubota Y, Takubo K, Hirashima M, Nagoshi N, Kishi K, Okuno Y, et al. Isolation and function of mouse tissue resident vascular precursors marked by myelin protein zero. J Exp Med. 2011;208:949-60 pubmed publisher
Link A, Hardie D, Favre S, Britschgi M, Adams D, Sixt M, et al. Association of T-zone reticular networks and conduits with ectopic lymphoid tissues in mice and humans. Am J Pathol. 2011;178:1662-75 pubmed publisher
Nolan Stevaux O, Truitt M, Pahler J, Olson P, Guinto C, Lee D, et al. Differential contribution to neuroendocrine tumorigenesis of parallel egfr signaling in cancer cells and pericytes. Genes Cancer. 2010;1:125-41 pubmed publisher
Gao J, Yan X, Li R, Liu Y, He W, Sun S, et al. Characterization of OP9 as authentic mesenchymal stem cell line. J Genet Genomics. 2010;37:475-82 pubmed publisher
Odaka C. Localization of mesenchymal cells in adult mouse thymus: their abnormal distribution in mice with disorganization of thymic medullary epithelium. J Histochem Cytochem. 2009;57:373-82 pubmed publisher
Lu K, Lamagna C, Bergers G. Chapter 3. Bone marrow-derived vascular progenitors and proangiogenic monocytes in tumors. Methods Enzymol. 2008;445:53-82 pubmed publisher
Katakai T, Suto H, Sugai M, Gonda H, Togawa A, Suematsu S, et al. Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs. J Immunol. 2008;181:6189-200 pubmed
Kobayashi A, Valerius M, Mugford J, Carroll T, Self M, Oliver G, et al. Six2 defines and regulates a multipotent self-renewing nephron progenitor population throughout mammalian kidney development. Cell Stem Cell. 2008;3:169-81 pubmed publisher
Treese C, Mittag A, Lange F, Tarnok A, Loesche A, Emmrich F, et al. Characterization of fibroblasts responsible for cartilage destruction in arthritis. Cytometry A. 2008;73:351-60 pubmed publisher
Jo N, Mailhos C, Ju M, Cheung E, Bradley J, Nishijima K, et al. Inhibition of platelet-derived growth factor B signaling enhances the efficacy of anti-vascular endothelial growth factor therapy in multiple models of ocular neovascularization. Am J Pathol. 2006;168:2036-53 pubmed
Kano M, Morishita Y, Iwata C, Iwasaka S, Watabe T, Ouchi Y, et al. VEGF-A and FGF-2 synergistically promote neoangiogenesis through enhancement of endogenous PDGF-B-PDGFRbeta signaling. J Cell Sci. 2005;118:3759-68 pubmed
product information
Product Type :
Antibody
Product Name :
CD140b (PDGFRB) Monoclonal Antibody (APB5), Functional Grade, eBioscience
Catalog # :
16-1402-82
Quantity :
100 µg
Price :
US 246
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 1 µg/test, Functional Assay: Assay-Dependent, Neutralization: Assay-Dependent
Species :
Mouse
Clone :
APB5
Isotype :
IgG2a, kappa
Storage :
4° C
Description :
PDGFRb is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. The gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1 µg/test, Functional Assay: Assay-Dependent, Neutralization: Assay-Dependent
Aliases :
AI528809; Beta platelet-derived growth factor receptor; beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140B; CD140b antigen; IBGC4; IMF1; JTK12; KOGS; PDGF beta chain; PDGF Receptor beta; Pdgfr; Pdgfr1; PDGFR-1; PDGFRB; PDGF-R-beta; PDGFR-beta; PENTT; platelet derived growth factor receptor beta; platelet derived growth factor receptor, beta polypeptide; platelet-derived growth factor receptor 1; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta variant 1; Platelet-derived growth factor receptor, beta; platelet-derived growth factor receptor, beta polypeptide
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA