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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD103 (Integrin alpha E) Monoclonal Antibody (2E7), Functional Grade, eBioscience
catalog :
16-1031-82
quantity :
100 µg
price :
US 229
clonality :
monoclonal
host :
hamsters
conjugate :
nonconjugated
clone name :
2E7
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 64
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:400; loading ...; fig 2f
Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s19
Mai J, Li Z, Xia X, Zhang J, Li J, Liu H, et al. Synergistic Activation of Antitumor Immunity by a Particulate Therapeutic Vaccine. Adv Sci (Weinh). 2021;8:2100166 pubmed publisher
  • flow cytometry; human; 1:100; loading ...; fig 7b
Gao M, Wang T, Ji L, Bai S, Tian L, Song H. Therapy With Carboplatin and Anti-PD-1 Antibodies Before Surgery Demonstrates Sustainable Anti-Tumor Effects for Secondary Cancers in Mice With Triple-Negative Breast Cancer. Front Immunol. 2020;11:366 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s8b
Sato Y, Bolzenius J, Eteleeb A, Su X, Maher C, Sehn J, et al. CD4+ T cells induce rejection of urothelial tumors after immune checkpoint blockade. JCI Insight. 2018;3: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1b
Macdougall C, Wood E, Loschko J, Scagliotti V, Cassidy F, Robinson M, et al. Visceral Adipose Tissue Immune Homeostasis Is Regulated by the Crosstalk between Adipocytes and Dendritic Cell Subsets. Cell Metab. 2018;27:588-601.e4 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6c
Turner D, Goldklang M, Cvetkovski F, Paik D, Trischler J, Barahona J, et al. Biased Generation and In Situ Activation of Lung Tissue-Resident Memory CD4 T Cells in the Pathogenesis of Allergic Asthma. J Immunol. 2018;200:1561-1569 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a
Leonardi I, Li X, Semon A, Li D, Doron I, Putzel G, et al. CX3CR1+ mononuclear phagocytes control immunity to intestinal fungi. Science. 2018;359:232-236 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2d
Whitney P, Makhlouf C, MacLeod B, Ma J, Gressier E, Greyer M, et al. Effective Priming of Herpes Simplex Virus-Specific CD8+ T Cells In Vivo Does Not Require Infected Dendritic Cells. J Virol. 2018;92: pubmed publisher
  • flow cytometry; mouse; fig s1b
Danahy D, Anthony S, Jensen I, Hartwig S, Shan Q, Xue H, et al. Polymicrobial sepsis impairs bystander recruitment of effector cells to infected skin despite optimal sensing and alarming function of skin resident memory CD8 T cells. PLoS Pathog. 2017;13:e1006569 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4
Torcellan T, Hampton H, Bailey J, Tomura M, Brink R, Chtanova T. In vivo photolabeling of tumor-infiltrating cells reveals highly regulated egress of T-cell subsets from tumors. Proc Natl Acad Sci U S A. 2017;114:5677-5682 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Meinicke H, Bremser A, Brack M, Schrenk K, Pircher H, Izcue A. KLRG1 impairs regulatory T-cell competitive fitness in the gut. Immunology. 2017;152:65-73 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Meinicke H, Bremser A, Brack M, Akeus P, Pearson C, Bullers S, et al. Tumour-associated changes in intestinal epithelial cells cause local accumulation of KLRG1+ GATA3+ regulatory T cells in mice. Immunology. 2017;152:74-88 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Shrestha B, You D, Saravia J, Siefker D, Jaligama S, Lee G, et al. IL-4R? on dendritic cells in neonates and Th2 immunopathology in respiratory syncytial virus infection. J Leukoc Biol. 2017;102:153-161 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s4b
Griffiths K, Ahmed M, Das S, Gopal R, Horne W, Connell T, et al. Targeting dendritic cells to accelerate T-cell activation overcomes a bottleneck in tuberculosis vaccine efficacy. Nat Commun. 2016;7:13894 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1f
Muschaweckh A, Buchholz V, Fellenzer A, Hessel C, König P, Tao S, et al. Antigen-dependent competition shapes the local repertoire of tissue-resident memory CD8+ T cells. J Exp Med. 2016;213:3075-3086 pubmed
  • flow cytometry; mouse; loading ...; fig 1b
Kretzer N, Theisen D, Tussiwand R, Briseño C, Grajales Reyes G, Wu X, et al. RAB43 facilitates cross-presentation of cell-associated antigens by CD8?+ dendritic cells. J Exp Med. 2016;213:2871-2883 pubmed
  • flow cytometry; mouse; fig 5
Man A, Gicheva N, Regoli M, Rowley G, De Cunto G, Wellner N, et al. CX3CR1+ Cell-Mediated Salmonella Exclusion Protects the Intestinal Mucosa during the Initial Stage of Infection. J Immunol. 2017;198:335-343 pubmed
  • flow cytometry; mouse; loading ...; fig 2f
Cummings R, Barbet G, Bongers G, Hartmann B, Gettler K, Muniz L, et al. Different tissue phagocytes sample apoptotic cells to direct distinct homeostasis programs. Nature. 2016;539:565-569 pubmed publisher
  • flow cytometry; mouse; fig 2c
Boddupalli C, Nair S, Gray S, Nowyhed H, Verma R, Gibson J, et al. ABC transporters and NR4A1 identify a quiescent subset of tissue-resident memory T cells. J Clin Invest. 2016;126:3905-3916 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2e
Chopra M, Biehl M, Steinfatt T, Brandl A, Kums J, Amich J, et al. Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion. J Exp Med. 2016;213:1881-900 pubmed publisher
  • flow cytometry; mouse; fig s3
Brandstätter O, Schanz O, Vorac J, König J, Mori T, Maruyama T, et al. Balancing intestinal and systemic inflammation through cell type-specific expression of the aryl hydrocarbon receptor repressor. Sci Rep. 2016;6:26091 pubmed publisher
  • flow cytometry; mouse; fig 2
Nieves W, Hung L, Oniskey T, Boon L, Foretz M, Viollet B, et al. Myeloid-Restricted AMPK?1 Promotes Host Immunity and Protects against IL-12/23p40-Dependent Lung Injury during Hookworm Infection. J Immunol. 2016;196:4632-40 pubmed publisher
  • flow cytometry; mouse; fig 2
Kabat A, Harrison O, Riffelmacher T, Moghaddam A, Pearson C, Laing A, et al. The autophagy gene Atg16l1 differentially regulates Treg and TH2 cells to control intestinal inflammation. elife. 2016;5:e12444 pubmed publisher
  • flow cytometry; mouse
Xin L, Jiang T, Kinder J, Ertelt J, Way S. Infection susceptibility and immune senescence with advancing age replicated in accelerated aging Lmna(Dhe) mice. Aging Cell. 2015;14:1122-6 pubmed publisher
  • flow cytometry; mouse; fig 3
Thiault N, Darrigues J, Adoue V, Gros M, Binet B, Pérals C, et al. Peripheral regulatory T lymphocytes recirculating to the thymus suppress the development of their precursors. Nat Immunol. 2015;16:628-34 pubmed publisher
  • flow cytometry; mouse; fig s5
Wiesner D, Specht C, Lee C, Smith K, Mukaremera L, Lee S, et al. Chitin recognition via chitotriosidase promotes pathologic type-2 helper T cell responses to cryptococcal infection. PLoS Pathog. 2015;11:e1004701 pubmed publisher
  • flow cytometry; mouse; fig 2
Wensveen F, Jelenčić V, Valentić S, Šestan M, Wensveen T, Theurich S, et al. NK cells link obesity-induced adipose stress to inflammation and insulin resistance. Nat Immunol. 2015;16:376-85 pubmed publisher
  • flow cytometry; mouse
Glatigny S, Duhen R, Arbelaez C, Kumari S, Bettelli E. Integrin alpha L controls the homing of regulatory T cells during CNS autoimmunity in the absence of integrin alpha 4. Sci Rep. 2015;5:7834 pubmed publisher
  • flow cytometry; mouse
Yin Y, Qin T, Wang X, Lin J, Yu Q, Yang Q. CpG DNA assists the whole inactivated H9N2 influenza virus in crossing the intestinal epithelial barriers via transepithelial uptake of dendritic cell dendrites. Mucosal Immunol. 2015;8:799-814 pubmed publisher
  • flow cytometry; mouse; fig 1
Guo X, Tanaka Y, Kondo M. Thymic precursors of TCRαβ(+)CD8αα(+) intraepithelial lymphocytes are negative for CD103. Immunol Lett. 2015;163:40-8 pubmed publisher
  • flow cytometry; mouse; fig s1
van Blijswijk J, Schraml B, Rogers N, Whitney P, Zelenay S, Acton S, et al. Altered lymph node composition in diphtheria toxin receptor-based mouse models to ablate dendritic cells. J Immunol. 2015;194:307-15 pubmed publisher
  • flow cytometry; mouse
Tassi I, Claudio E, Wang H, Tang W, Ha H, Saret S, et al. The NF-κB regulator Bcl-3 governs dendritic cell antigen presentation functions in adaptive immunity. J Immunol. 2014;193:4303-11 pubmed publisher
  • flow cytometry; mouse; fig 2
Berod L, Stüve P, Varela F, Behrends J, Swallow M, Kruse F, et al. Rapid rebound of the Treg compartment in DEREG mice limits the impact of Treg depletion on mycobacterial burden, but prevents autoimmunity. PLoS ONE. 2014;9:e102804 pubmed publisher
  • flow cytometry; mouse
Longman R, Diehl G, Victorio D, Huh J, Galan C, Miraldi E, et al. CX?CR1? mononuclear phagocytes support colitis-associated innate lymphoid cell production of IL-22. J Exp Med. 2014;211:1571-83 pubmed publisher
  • flow cytometry; mouse; fig 8
Satpathy A, Briseño C, Lee J, Ng D, Manieri N, Kc W, et al. Notch2-dependent classical dendritic cells orchestrate intestinal immunity to attaching-and-effacing bacterial pathogens. Nat Immunol. 2013;14:937-48 pubmed publisher
  • flow cytometry; mouse; fig 5b
Wu X, Satpathy A, Kc W, Liu P, Murphy T, Murphy K. Bcl11a controls Flt3 expression in early hematopoietic progenitors and is required for pDC development in vivo. PLoS ONE. 2013;8:e64800 pubmed publisher
  • flow cytometry; mouse
Powell N, Walker A, Stolarczyk E, Canavan J, Gökmen M, Marks E, et al. The transcription factor T-bet regulates intestinal inflammation mediated by interleukin-7 receptor+ innate lymphoid cells. Immunity. 2012;37:674-84 pubmed publisher
  • flow cytometry; mouse
Zhang N, Bevan M. TGF-? signaling to T cells inhibits autoimmunity during lymphopenia-driven proliferation. Nat Immunol. 2012;13:667-73 pubmed publisher
  • flow cytometry; mouse
Wollenberg I, Agua Doce A, Hernandez A, Almeida C, Oliveira V, Faro J, et al. Regulation of the germinal center reaction by Foxp3+ follicular regulatory T cells. J Immunol. 2011;187:4553-60 pubmed publisher
  • flow cytometry; mouse
MANICONE A, Huizar I, McGuire J. Matrilysin (Matrix Metalloproteinase-7) regulates anti-inflammatory and antifibrotic pulmonary dendritic cells that express CD103 (alpha(E)beta(7)-integrin). Am J Pathol. 2009;175:2319-31 pubmed publisher
  • flow cytometry; mouse
Saito K, Torii M, Ma N, Tsuchiya T, Wang L, Hori T, et al. Differential regulatory function of resting and preactivated allergen-specific CD4+ CD25+ regulatory T cells in Th2-type airway inflammation. J Immunol. 2008;181:6889-97 pubmed
  • flow cytometry; mouse; fig 1C
Stephens G, Andersson J, Shevach E. Distinct subsets of FoxP3+ regulatory T cells participate in the control of immune responses. J Immunol. 2007;178:6901-11 pubmed
Vonderhaar E, Barnekow N, McAllister D, McOlash L, Eid M, Riese M, et al. STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer. Cell Mol Gastroenterol Hepatol. 2021;12:41-58 pubmed publisher
Leach S, Gibbings S, Tewari A, Atif S, Vestal B, Danhorn T, et al. Human and Mouse Transcriptome Profiling Identifies Cross-Species Homology in Pulmonary and Lymph Node Mononuclear Phagocytes. Cell Rep. 2020;33:108337 pubmed publisher
Tong D, Zhang L, Ning F, Xu Y, Hu X, Shi Y. Contact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survival. Protein Cell. 2020;11:108-123 pubmed publisher
Rosato P, Wijeyesinghe S, Stolley J, Nelson C, Davis R, Manlove L, et al. Virus-specific memory T cells populate tumors and can be repurposed for tumor immunotherapy. Nat Commun. 2019;10:567 pubmed publisher
Funk K, Klein R. CSF1R antagonism limits local restimulation of antiviral CD8+ T cells during viral encephalitis. J Neuroinflammation. 2019;16:22 pubmed publisher
Kawasaki T, Ito K, Miyata H, Akira S, Kawai T. Deletion of PIKfyve alters alveolar macrophage populations and exacerbates allergic inflammation in mice. EMBO J. 2017;36:1707-1718 pubmed publisher
Pelly V, Coomes S, Kannan Y, Gialitakis M, Entwistle L, Perez Lloret J, et al. Interleukin 4 promotes the development of ex-Foxp3 Th2 cells during immunity to intestinal helminths. J Exp Med. 2017;214:1809-1826 pubmed publisher
Lyons Cohen M, Thomas S, Cook D, Nakano H. Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells. J Vis Exp. 2017;: pubmed publisher
Chen L, Wilson J, Koenigsknecht M, Chou W, Montgomery S, Truax A, et al. NLRP12 attenuates colon inflammation by maintaining colonic microbial diversity and promoting protective commensal bacterial growth. Nat Immunol. 2017;18:541-551 pubmed publisher
Oh J, Oh D, Jung H, Lee H. A mechanism for the induction of type 2 immune responses by a protease allergen in the genital tract. Proc Natl Acad Sci U S A. 2017;114:E1188-E1195 pubmed publisher
Marshall N, Vong A, Devarajan P, Brauner M, Kuang Y, Nayar R, et al. NKG2C/E Marks the Unique Cytotoxic CD4 T Cell Subset, ThCTL, Generated by Influenza Infection. J Immunol. 2017;198:1142-1155 pubmed publisher
Chudnovskiy A, Mortha A, Kana V, Kennard A, Ramírez J, Rahman A, et al. Host-Protozoan Interactions Protect from Mucosal Infections through Activation of the Inflammasome. Cell. 2016;167:444-456.e14 pubmed publisher
Steinbach K, Vincenti I, Kreutzfeldt M, Page N, Muschaweckh A, Wagner I, et al. Brain-resident memory T cells represent an autonomous cytotoxic barrier to viral infection. J Exp Med. 2016;213:1571-87 pubmed publisher
Menendez C, Jinkins J, Carr D. Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency. J Immunol. 2016;197:1262-75 pubmed publisher
Ochi T, Feng Y, Kitamoto S, Nagao Kitamoto H, Kuffa P, Atarashi K, et al. Diet-dependent, microbiota-independent regulation of IL-10-producing lamina propria macrophages in the small intestine. Sci Rep. 2016;6:27634 pubmed publisher
Kim M, Taparowsky E, Kim C. Retinoic Acid Differentially Regulates the Migration of Innate Lymphoid Cell Subsets to the Gut. Immunity. 2015;43:107-19 pubmed publisher
Clatworthy M, Aronin C, Mathews R, Morgan N, Smith K, Germain R. Immune complexes stimulate CCR7-dependent dendritic cell migration to lymph nodes. Nat Med. 2014;20:1458-63 pubmed publisher
Qin Q, Wang Z, Pan P, Cao Z, Xia Q, Tan H, et al. Lung dendritic cells undergo maturation and polarization towards a T helper type 2-stimulating phenotype in a mouse model of asthma: Role of nerve growth factor. Exp Ther Med. 2014;8:1402-1408 pubmed
Thomas D, Wong F, Zaccone P, Green E, Wallberg M. Protection of islet grafts through transforming growth factor-?-induced tolerogenic dendritic cells. Diabetes. 2013;62:3132-42 pubmed publisher
Sun C, Hall J, Blank R, Bouladoux N, Oukka M, Mora J, et al. Small intestine lamina propria dendritic cells promote de novo generation of Foxp3 T reg cells via retinoic acid. J Exp Med. 2007;204:1775-85 pubmed
Sather B, Treuting P, Perdue N, Miazgowicz M, Fontenot J, Rudensky A, et al. Altering the distribution of Foxp3(+) regulatory T cells results in tissue-specific inflammatory disease. J Exp Med. 2007;204:1335-47 pubmed
Suzuki R, Nakao A, Kanamaru Y, Okumura K, Ogawa H, Ra C. Localization of intestinal intraepithelial T lymphocytes involves regulation of alphaEbeta7 expression by transforming growth factor-beta. Int Immunol. 2002;14:339-45 pubmed
product information
Product Type :
Antibody
Product Name :
CD103 (Integrin alpha E) Monoclonal Antibody (2E7), Functional Grade, eBioscience
Catalog # :
16-1031-82
Quantity :
100 µg
Price :
US 229
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Armenian Hamster
Reactivity :
Mouse
Applications :
Flow Cytometry: 1 µg/test, Functional Assay: Assay-Dependent
Species :
Mouse
Clone :
2E7
Isotype :
IgG
Storage :
4° C
Description :
CD103 (ITGAE, Integrin alpha E) is molecule that is expressed on mucosa-associated T lymphocytes and activated cells and on subet of TGF beta -1 cells. In particular, CD103 includes a 150kDa alpha chain and a 120kDa beta chain. CD103 is expressed by 0.5 to 2% of resting lymphocytes in peripheral blood and lymphoid organs. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is part of the integrin family which are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. ITGAE encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, CD103 protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. CD103 is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1 µg/test, Functional Assay: Assay-Dependent
Aliases :
A530055J10; alpha-E1; alpha-M290; aM290; antigen CD103; antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103; DC; DCs; HML-1 antigen; human mucosal lymphocyte antigen 1, alpha polypeptide; HUMINAE; integrin alpha E; integrin alpha E, epithelial-associated; integrin alpha E1, epithelial-associated; integrin alpha M290; Integrin alpha-E; Integrin alpha-E heavy chain; Integrin alpha-E light chain; Integrin alpha-IEL; integrin subunit alpha E; integrin, alpha E; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide); integrin, alpha E, epithelial-associated; Intregrin alpha E2; Itgae; MGC141996; Mucosal lymphocyte 1 antigen
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA