This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD86 (B7-2) Monoclonal Antibody (PO3.1), Functional Grade, eBioscience
catalog :
16-0861-81
quantity :
50 µg
price :
US 136
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
PO3.1
reactivity :
human, mouse
application :
neutralization, flow cytometry
citations: 21
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:80; loading ...; fig 1a
Kouwenberg M, Jacobs C, van der Vlag J, Hilbrands L. Allostimulatory Effects of Dendritic Cells with Characteristic Features of a Regulatory Phenotype. PLoS ONE. 2016;11:e0159986 pubmed publisher
  • flow cytometry; mouse
Ikeda T, Hirata S, Takamatsu K, Haruta M, Tsukamoto H, Ito T, et al. Suppression of Th1-mediated autoimmunity by embryonic stem cell-derived dendritic cells. PLoS ONE. 2014;9:e115198 pubmed publisher
  • flow cytometry; mouse
Kiesel J, Buchwald Z, Aurora R. Cross-presentation by osteoclasts induces FoxP3 in CD8+ T cells. J Immunol. 2009;182:5477-87 pubmed publisher
  • flow cytometry; mouse; fig 2
Zhang J, Raper A, Sugita N, Hingorani R, Salio M, Palmowski M, et al. Characterization of Siglec-H as a novel endocytic receptor expressed on murine plasmacytoid dendritic cell precursors. Blood. 2006;107:3600-8 pubmed
  • flow cytometry; human
  • flow cytometry; mouse; fig 5
Hoffmann P, Kench J, Vondracek A, Kruk E, Daleke D, Jordan M, et al. Interaction between phosphatidylserine and the phosphatidylserine receptor inhibits immune responses in vivo. J Immunol. 2005;174:1393-404 pubmed
Du X, Tang F, Liu M, Su J, Zhang Y, Wu W, et al. A reappraisal of CTLA-4 checkpoint blockade in cancer immunotherapy. Cell Res. 2018;28:416-432 pubmed publisher
van Nieuwenhuijze A, Dooley J, Humblet Baron S, Sreenivasan J, Koenders M, Schlenner S, et al. Defective germinal center B-cell response and reduced arthritic pathology in microRNA-29a-deficient mice. Cell Mol Life Sci. 2017;74:2095-2106 pubmed publisher
Lee P, Zhu Z, Hachmann J, Nojima T, Kitamura D, Salvesen G, et al. Differing Requirements for MALT1 Function in Peripheral B Cell Survival and Differentiation. J Immunol. 2017;198:1066-1080 pubmed publisher
Maj T, Slawek A, Chelmonska Soyta A. CD80 and CD86 costimulatory molecules differentially regulate OT-II CD4? T lymphocyte proliferation and cytokine response in cocultures with antigen-presenting cells derived from pregnant and pseudopregnant mice. Mediators Inflamm. 2014;2014:769239 pubmed publisher
Lucas C, Cordero Nieves H, Erbe R, McAlees J, Bhatia S, Hodes R, et al. Prohibitins and the cytoplasmic domain of CD86 cooperate to mediate CD86 signaling in B lymphocytes. J Immunol. 2013;190:723-36 pubmed publisher
Nakajima K, Yamashita T, Kita T, Takeda M, Sasaki N, Kasahara K, et al. Orally administered eicosapentaenoic acid induces rapid regression of atherosclerosis via modulating the phenotype of dendritic cells in LDL receptor-deficient mice. Arterioscler Thromb Vasc Biol. 2011;31:1963-72 pubmed publisher
Takeda M, Yamashita T, Sasaki N, Nakajima K, Kita T, Shinohara M, et al. Oral administration of an active form of vitamin D3 (calcitriol) decreases atherosclerosis in mice by inducing regulatory T cells and immature dendritic cells with tolerogenic functions. Arterioscler Thromb Vasc Biol. 2010;30:2495-503 pubmed publisher
Peters M, Dudziak K, Stiehm M, Bufe A. T-cell polarization depends on concentration of the danger signal used to activate dendritic cells. Immunol Cell Biol. 2010;88:537-44 pubmed publisher
Zhang J, Zou L, Liu Q, Li J, Zhou J, Wang Y, et al. Rapid generation of dendritic cell specific transgenic mice by lentiviral vectors. Transgenic Res. 2009;18:921-31 pubmed publisher
Yi H, Yu X, Gao P, Wang Y, Baek S, Chen X, et al. Pattern recognition scavenger receptor SRA/CD204 down-regulates Toll-like receptor 4 signaling-dependent CD8 T-cell activation. Blood. 2009;113:5819-28 pubmed publisher
Siracusa M, Reece J, Urban J, Scott A. Dynamics of lung macrophage activation in response to helminth infection. J Leukoc Biol. 2008;84:1422-33 pubmed publisher
Porubsky S, Speak A, Luckow B, Cerundolo V, Platt F, Grone H. Normal development and function of invariant natural killer T cells in mice with isoglobotrihexosylceramide (iGb3) deficiency. Proc Natl Acad Sci U S A. 2007;104:5977-82 pubmed
Wang C, McDonald K, McDonough J, Newberry R. Murine isolated lymphoid follicles contain follicular B lymphocytes with a mucosal phenotype. Am J Physiol Gastrointest Liver Physiol. 2006;291:G595-604 pubmed
Podojil J, Kin N, Sanders V. CD86 and beta2-adrenergic receptor signaling pathways, respectively, increase Oct-2 and OCA-B Expression and binding to the 3'-IgH enhancer in B cells. J Biol Chem. 2004;279:23394-404 pubmed
Hausl C, Ahmad R, Schwarz H, Muchitsch E, Turecek P, Dorner F, et al. Preventing restimulation of memory B cells in hemophilia A: a potential new strategy for the treatment of antibody-dependent immune disorders. Blood. 2004;104:115-22 pubmed
Nakajima A, Azuma M, Kodera S, Nuriya S, Terashi A, Abe M, et al. Preferential dependence of autoantibody production in murine lupus on CD86 costimulatory molecule. Eur J Immunol. 1995;25:3060-9 pubmed
product information
Product Type :
Antibody
Product Name :
CD86 (B7-2) Monoclonal Antibody (PO3.1), Functional Grade, eBioscience
Catalog # :
16-0861-81
Quantity :
50 µg
Price :
US 136
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.25 µg/test, Functional Assay: Assay-Dependent, Neutralization: Assay-Dependent
Species :
Mouse
Clone :
PO3.1
Isotype :
IgG2b, kappa
Storage :
4° C
Description :
CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.25 µg/test, Functional Assay: Assay-Dependent, Neutralization: Assay-Dependent
Aliases :
activation B7-2 antigen; B lymphocyte activation antigen B72; B7; B7.2; B70; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86; CD86 antigen; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 antigen precursor; CD86 molecule; CLS1; CTLA-4 counter-receptor B7.2; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly58; Ly-58; MB7; MB7-2; membrane glycoprotein; MGC34413; T-lymphocyte activation antigen CD86; TS/A-2
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA