product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD80 (B7-1) Monoclonal Antibody (16-10A1), Functional Grade, eBioscience
catalog :
16-0801-85
quantity :
500 µg
price :
US 347
clonality :
monoclonal
host :
hamsters
conjugate :
nonconjugated
clone name :
16-10A1
reactivity :
human, mouse, dogs, pigs
application :
neutralization, flow cytometry
more info or order :
citations: 239
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:300; loading ...; fig 3m
Stebegg M, Bignon A, Hill D, Silva Cayetano A, Krueger C, Vanderleyden I, et al. Rejuvenating conventional dendritic cells and T follicular helper cell formation after vaccination. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Mizuno R, Sugiura D, Shimizu K, Maruhashi T, Watada M, Okazaki I, et al. PD-1 Primarily Targets TCR Signal in the Inhibition of Functional T Cell Activation. Front Immunol. 2019;10:630 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6c
Lee Y, Ju J, Shon W, Oh S, Min C, Kang M, et al. Skewed Dendritic Cell Differentiation of MyD88-Deficient Donor Bone Marrow Cells, Instead of Massive Expansion as Myeloid-Derived Suppressor Cells, Aggravates GVHD. Immune Netw. 2018;18:e44 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3h
Düsedau H, Kleveman J, Figueiredo C, Biswas A, Steffen J, Kliche S, et al. p75NTR regulates brain mononuclear cell function and neuronal structure in Toxoplasma infection-induced neuroinflammation. Glia. 2019;67:193-211 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2
Kaplanov I, Carmi Y, Kornetsky R, Shemesh A, Shurin G, Shurin M, et al. Blocking IL-1β reverses the immunosuppression in mouse breast cancer and synergizes with anti-PD-1 for tumor abrogation. Proc Natl Acad Sci U S A. 2019;116:1361-1369 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e5c
Du X, Wen J, Wang Y, Karmaus P, Khatamian A, Tan H, et al. Hippo/Mst signalling couples metabolic state and immune function of CD8α+ dendritic cells. Nature. 2018;558:141-145 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5d
Shrestha B, You D, Saravia J, Siefker D, Jaligama S, Lee G, et al. IL-4R? on dendritic cells in neonates and Th2 immunopathology in respiratory syncytial virus infection. J Leukoc Biol. 2017;102:153-161 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Inoue T, Shinnakasu R, Ise W, Kawai C, Egawa T, Kurosaki T. The transcription factor Foxo1 controls germinal center B cell proliferation in response to T cell help. J Exp Med. 2017;214:1181-1198 pubmed publisher
  • flow cytometry; mouse; fig s2b
Wang S, Xia P, Chen Y, Huang G, Xiong Z, Liu J, et al. Natural Killer-like B Cells Prime Innate Lymphocytes against Microbial Infection. Immunity. 2016;45:131-44 pubmed publisher
  • flow cytometry; mouse; 1:100; fig 2
Schachtner H, Weimershaus M, Stache V, Plewa N, Legler D, Höpken U, et al. Loss of Gadkin Affects Dendritic Cell Migration In Vitro. PLoS ONE. 2015;10:e0143883 pubmed publisher
  • flow cytometry; mouse
Sun L, Hua Y, Vergarajauregui S, Diab H, Puertollano R. Novel Role of TRPML2 in the Regulation of the Innate Immune Response. J Immunol. 2015;195:4922-32 pubmed publisher
  • flow cytometry; mouse
Carmi Y, Spitzer M, Linde I, Burt B, Prestwood T, Perlman N, et al. Allogeneic IgG combined with dendritic cell stimuli induce antitumour T-cell immunity. Nature. 2015;521:99-104 pubmed publisher
  • flow cytometry; mouse; fig s3
Pannu J, Belle J, Forster M, Duerr C, Shen S, Kane L, et al. Ubiquitin specific protease 21 is dispensable for normal development, hematopoiesis and lymphocyte differentiation. PLoS ONE. 2015;10:e0117304 pubmed publisher
  • flow cytometry; mouse; fig 3
Sun H, Zhang J, Chen F, Chen X, Zhou Z, Wang H. Activation of RAW264.7 macrophages by the polysaccharide from the roots of Actinidia eriantha and its molecular mechanisms. Carbohydr Polym. 2015;121:388-402 pubmed publisher
  • flow cytometry; mouse
Ikeda T, Hirata S, Takamatsu K, Haruta M, Tsukamoto H, Ito T, et al. Suppression of Th1-mediated autoimmunity by embryonic stem cell-derived dendritic cells. PLoS ONE. 2014;9:e115198 pubmed publisher
  • flow cytometry; mouse; fig 2
McDonnell A, Lesterhuis W, Khong A, Nowak A, Lake R, Currie A, et al. Tumor-infiltrating dendritic cells exhibit defective cross-presentation of tumor antigens, but is reversed by chemotherapy. Eur J Immunol. 2015;45:49-59 pubmed publisher
  • flow cytometry; mouse; fig 4
Rauen J, Kreer C, Paillard A, van Duikeren S, Benckhuijsen W, Camps M, et al. Enhanced cross-presentation and improved CD8+ T cell responses after mannosylation of synthetic long peptides in mice. PLoS ONE. 2014;9:e103755 pubmed publisher
  • flow cytometry; mouse
Skrnjug I, Rueckert C, Libanova R, Lienenklaus S, Weiss S, Guzman C. The mucosal adjuvant cyclic di-AMP exerts immune stimulatory effects on dendritic cells and macrophages. PLoS ONE. 2014;9:e95728 pubmed publisher
  • flow cytometry; mouse; fig 3
Harimoto H, Shimizu M, Nakagawa Y, Nakatsuka K, Wakabayashi A, Sakamoto C, et al. Inactivation of tumor-specific CD8? CTLs by tumor-infiltrating tolerogenic dendritic cells. Immunol Cell Biol. 2013;91:545-55 pubmed publisher
  • flow cytometry; mouse
Purtha W, Tedder T, Johnson S, Bhattacharya D, Diamond M. Memory B cells, but not long-lived plasma cells, possess antigen specificities for viral escape mutants. J Exp Med. 2011;208:2599-606 pubmed publisher
  • flow cytometry; mouse; fig 4
Ellestad K, Tsutsui S, Noorbakhsh F, Warren K, Yong V, Pittman Q, et al. Early life exposure to lipopolysaccharide suppresses experimental autoimmune encephalomyelitis by promoting tolerogenic dendritic cells and regulatory T cells. J Immunol. 2009;183:298-309 pubmed publisher
  • flow cytometry; mouse
Kiesel J, Buchwald Z, Aurora R. Cross-presentation by osteoclasts induces FoxP3 in CD8+ T cells. J Immunol. 2009;182:5477-87 pubmed publisher
  • flow cytometry; mouse
Hamazaki Y, Fujita H, Kobayashi T, Choi Y, Scott H, Matsumoto M, et al. Medullary thymic epithelial cells expressing Aire represent a unique lineage derived from cells expressing claudin. Nat Immunol. 2007;8:304-11 pubmed
  • flow cytometry; mouse; fig 5
  • flow cytometry; human
Hoffmann P, Kench J, Vondracek A, Kruk E, Daleke D, Jordan M, et al. Interaction between phosphatidylserine and the phosphatidylserine receptor inhibits immune responses in vivo. J Immunol. 2005;174:1393-404 pubmed
Wang S, Zaitoun I, Darjatmoko S, Sheibani N, Sorenson C. Bim Expression Promotes the Clearance of Mononuclear Phagocytes during Choroidal Neovascularization, Mitigating Scar Formation in Mice. Life (Basel). 2022;12: pubmed publisher
Jhan M, Chen C, Shen T, Tseng P, Wang Y, Satria R, et al. Polarization of Type 1 Macrophages Is Associated with the Severity of Viral Encephalitis Caused by Japanese Encephalitis Virus and Dengue Virus. Cells. 2021;10: pubmed publisher
Nascimento Da Conceicao V, Sun Y, Ramachandran K, Chauhan A, Raveendran A, Venkatesan M, et al. Resolving macrophage polarization through distinct Ca2+ entry channel that maintains intracellular signaling and mitochondrial bioenergetics. iScience. 2021;24:103339 pubmed publisher
Gao L, Jiang W, Liu H, Chen Z, Lin Y. Receptor-selective interleukin-4 mutein attenuates laser-induced choroidal neovascularization through the regulation of macrophage polarization in mice. Exp Ther Med. 2021;22:1367 pubmed publisher
Keum H, Kim D, Kim J, Kim T, Whang C, Jung W, et al. A bilirubin-derived nanomedicine attenuates the pathological cascade of pulmonary fibrosis. Biomaterials. 2021;275:120986 pubmed publisher
Wang J, Chen Q, Zhang Z, Wang S, Wang Y, Xiang M, et al. Azithromycin alleviates systemic lupus erythematosus via the promotion of M2 polarisation in lupus mice. Cell Death Discov. 2021;7:82 pubmed publisher
Kim H, Park J, Park J, Yasmin F, Kim C, Oh S, et al. Cell-permeable transgelin-2 as a potent therapeutic for dendritic cell-based cancer immunotherapy. J Hematol Oncol. 2021;14:43 pubmed publisher
Shen P, Li X, Deng S, Zhao L, Zhang Y, Deng X, et al. Urolithin A ameliorates experimental autoimmune encephalomyelitis by targeting aryl hydrocarbon receptor. EBioMedicine. 2021;64:103227 pubmed publisher
Chen H, Tan J, Li X, Li H, Wu W, Wu Y, et al. Myeloid and plasmacytoid dendritic cell combined vaccines loaded with heat-treated tumor cell lysates enhance antitumor activity in murine lung cancer. Oncol Lett. 2021;21:90 pubmed publisher
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Liu Y, Yin Z, Lu P, Ma Y, Luo B, Xiang L, et al. Lung Carcinoma Cells Secrete Exosomal MALAT1 to Inhibit Dendritic Cell Phagocytosis, Inflammatory Response, Costimulatory Molecule Expression and Promote Dendritic Cell Autophagy via AKT/mTOR Pathway. Onco Targets Ther. 2020;13:10693-10705 pubmed publisher
Sun P, Kim Y, Lee H, Kim J, Han B, Go E, et al. Carrot Pomace Polysaccharide (CPP) Improves Influenza Vaccine Efficacy in Immunosuppressed Mice via Dendritic Cell Activation. Nutrients. 2020;12: pubmed publisher
Li G, Yang L, Li D, Zhang J, Du L, Xia L, et al. Effects of combined treatment with PD‑L1 Ig and CD40L mAb on immune tolerance in the CBA/J x DBA/2 mouse model. Mol Med Rep. 2020;21:1789-1798 pubmed publisher
Chen G, Du J, Nie Q, Du Y, Liu S, Liu D, et al. Plasmodium yoelii 17XL infection modified maturation and function of dendritic cells by skewing Tregs and amplificating Th17. BMC Infect Dis. 2020;20:266 pubmed publisher
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Pan Y, Yu Y, Lin C, Lanier L, Chu C. Fc?RI ?-Chain Negatively Modulates Dectin-1 Responses in Dendritic Cells. Front Immunol. 2017;8:1424 pubmed publisher
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Baik S, Jenkinson E, Lane P, Anderson G, Jenkinson W. Generation of both cortical and Aire(+) medullary thymic epithelial compartments from CD205(+) progenitors. Eur J Immunol. 2013;43:589-94 pubmed publisher
Mittal A, Raber A, Schaefer U, Weissmann S, Ebensen T, Schulze K, et al. Non-invasive delivery of nanoparticles to hair follicles: a perspective for transcutaneous immunization. Vaccine. 2013;31:3442-51 pubmed publisher
Wanderley J, Thorpe P, Barcinski M, Soong L. Phosphatidylserine exposure on the surface of Leishmania amazonensis amastigotes modulates in vivo infection and dendritic cell function. Parasite Immunol. 2013;35:109-119 pubmed publisher
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Corbitt N, Kimura S, Isse K, Specht S, Chedwick L, Rosborough B, et al. Gut bacteria drive Kupffer cell expansion via MAMP-mediated ICAM-1 induction on sinusoidal endothelium and influence preservation-reperfusion injury after orthotopic liver transplantation. Am J Pathol. 2013;182:180-91 pubmed publisher
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Kulinski J, Leonardo S, Mounce B, Malherbe L, Gauld S, Tarakanova V. Ataxia telangiectasia mutated kinase controls chronic gammaherpesvirus infection. J Virol. 2012;86:12826-37 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
CD80 (B7-1) Monoclonal Antibody (16-10A1), Functional Grade, eBioscience
Catalog # :
16-0801-85
Quantity :
500 µg
Pricing :
US 347
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Armenian Hamster
Reactivity :
Canine, Mouse, Porcine
Applications :
Flow Cytometry: 0.5 µg/test, Functional assay: Assay-Dependent, Neutralization: Assay-Dependent
Species :
Canine, Mouse, Porcine
Clone :
16-10A1
Isotype :
IgG
Storage :
4° C
Description :
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test, Functional assay: Assay-Dependent, Neutralization: Assay-Dependent
Aliases :
Activation B7-1 antigen; B7; B7 protein; B7.1; B71; B7-1; B7-1 protein; B7-1 protein precursor; BB1; B-lymphocyte activation antigen B7; Cd28l; CD28LG; CD28LG1; CD80; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 anitgen; CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); Cd80 molecule; CD80 protein precursor; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; Ly53; Ly-53; MIC17; sCD 80; sCD80; secreted B7-1 protein precursor; soluble CD 80; soluble CD80; T-cell costimulatory molecule; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1; TSA1
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA