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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), Functional Grade, eBioscience™
catalog :
16-0492-85
quantity :
500µg
price :
US 323
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
R1-2
reactivity :
mouse
application :
flow cytometry, blocking or activating experiments
more info or order :
citations: 50
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 6c
Lewis G, Wehrens E, Labarta Bajo L, Streeck H, Zuniga E. TGF-? receptor maintains CD4 T helper cell identity during chronic viral infections. J Clin Invest. 2016;126:3799-3813 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Hwang S, Cobb D, Bhadra R, Youngblood B, Khan I. Blimp-1-mediated CD4 T cell exhaustion causes CD8 T cell dysfunction during chronic toxoplasmosis. J Exp Med. 2016;213:1799-818 pubmed publisher
  • blocking or activating experiments; mouse; fig 8
Brinkman C, Iwami D, Hritzo M, Xiong Y, Ahmad S, Simon T, et al. Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration. Nat Commun. 2016;7:12021 pubmed publisher
  • flow cytometry; mouse; 1:100; fig s4
Ludigs K, Jandus C, Utzschneider D, Staehli F, Bessoles S, Dang A, et al. NLRC5 shields T lymphocytes from NK-cell-mediated elimination under inflammatory conditions. Nat Commun. 2016;7:10554 pubmed publisher
  • flow cytometry; mouse; fig 6
Zhang Y, Wu B, Metelli A, Thaxton J, Hong F, Rachidi S, et al. GP96 is a GARP chaperone and controls regulatory T cell functions. J Clin Invest. 2015;125:859-69 pubmed publisher
  • flow cytometry; mouse
Glatigny S, Duhen R, Arbelaez C, Kumari S, Bettelli E. Integrin alpha L controls the homing of regulatory T cells during CNS autoimmunity in the absence of integrin alpha 4. Sci Rep. 2015;5:7834 pubmed publisher
  • flow cytometry; mouse
Hong F, Liu B, Chiosis G, Gewirth D, Li Z. ?7 helix region of ?I domain is crucial for integrin binding to endoplasmic reticulum chaperone gp96: a potential therapeutic target for cancer metastasis. J Biol Chem. 2013;288:18243-8 pubmed publisher
Rolot M, Dougall A, Chetty A, Javaux J, Chen T, Xiao X, et al. Helminth-induced IL-4 expands bystander memory CD8+ T cells for early control of viral infection. Nat Commun. 2018;9:4516 pubmed publisher
Kobori T, Hamasaki S, Kitaura A, Yamazaki Y, Nishinaka T, Niwa A, et al. Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis. Front Immunol. 2018;9:334 pubmed publisher
Deniset J, Surewaard B, Lee W, Kubes P. Splenic Ly6Ghigh mature and Ly6Gint immature neutrophils contribute to eradication of S. pneumoniae. J Exp Med. 2017;214:1333-1350 pubmed publisher
Fontana M, Baccarella A, Craft J, Boyle M, McIntyre T, Wood M, et al. A Novel Model of Asymptomatic Plasmodium Parasitemia That Recapitulates Elements of the Human Immune Response to Chronic Infection. PLoS ONE. 2016;11:e0162132 pubmed publisher
Vila Leahey A, Rogers D, Marshall J. The impact of ranitidine on monocyte responses in the context of solid tumors. Oncotarget. 2016;7:10891-904 pubmed publisher
Shimizu K, Sakurai F, Tomita K, Nagamoto Y, Nakamura S, Katayama K, et al. Suppression of leaky expression of adenovirus genes by insertion of microRNA-targeted sequences in the replication-incompetent adenovirus vector genome. Mol Ther Methods Clin Dev. 2014;1:14035 pubmed publisher
Shindo Y, Unsinger J, Burnham C, Green J, Hotchkiss R. Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression. Shock. 2015;43:334-43 pubmed publisher
Langhauser F, Kraft P, Göb E, Leinweber J, Schuhmann M, Lorenz K, et al. Blocking of ?4 integrin does not protect from acute ischemic stroke in mice. Stroke. 2014;45:1799-806 pubmed publisher
Marandu T, Finsterbusch K, Kröger A, Cicin Sain L. Mouse CMV infection delays antibody class switch upon an unrelated virus challenge. Exp Gerontol. 2014;54:101-8 pubmed publisher
Renkema K, Li G, Wu A, Smithey M, Nikolich Žugich J. Two separate defects affecting true naive or virtual memory T cell precursors combine to reduce naive T cell responses with aging. J Immunol. 2014;192:151-9 pubmed publisher
Beus J, Hashmi S, Selvaraj S, Duan D, Stempora L, Monday S, et al. Heterologous immunity triggered by a single, latent virus in Mus musculus: combined costimulation- and adhesion- blockade decrease rejection. PLoS ONE. 2013;8:e71221 pubmed publisher
Brinkman C, Rouhani S, Srinivasan N, Engelhard V. Peripheral tissue homing receptors enable T cell entry into lymph nodes and affect the anatomical distribution of memory cells. J Immunol. 2013;191:2412-25 pubmed publisher
Karlsson F, Martinez N, Gray L, Zhang S, Tsunoda I, Grisham M. Therapeutic evaluation of ex vivo-generated versus natural regulatory T-cells in a mouse model of chronic gut inflammation. Inflamm Bowel Dis. 2013;19:2282-94 pubmed publisher
Kavanagh D, Yemm A, Zhao Y, Frampton J, Kalia N. Mechanisms of adhesion and subsequent actions of a haematopoietic stem cell line, HPC-7, in the injured murine intestinal microcirculation in vivo. PLoS ONE. 2013;8:e59150 pubmed publisher
Virtuoso L, Harden J, Sotomayor P, Sigurdson W, Yoshimura F, Egilmez N, et al. Characterization of iNOS(+) Neutrophil-like ring cell in tumor-bearing mice. J Transl Med. 2012;10:152 pubmed publisher
Shokeen M, Zheleznyak A, Wilson J, Jiang M, Liu R, Ferdani R, et al. Molecular imaging of very late antigen-4 (?4?1 integrin) in the premetastatic niche. J Nucl Med. 2012;53:779-86 pubmed publisher
Bankoti R, Gupta K, Levchenko A, Stager S. Marginal zone B cells regulate antigen-specific T cell responses during infection. J Immunol. 2012;188:3961-71 pubmed publisher
Nobuhisa I, Yamasaki S, Ramadan A, Taga T. CD45(low)c-Kit(high) cells have hematopoietic properties in the mouse aorta-gonad-mesonephros region. Exp Cell Res. 2012;318:705-15 pubmed publisher
Glatigny S, Duhen R, Oukka M, Bettelli E. Cutting edge: loss of ?4 integrin expression differentially affects the homing of Th1 and Th17 cells. J Immunol. 2011;187:6176-9 pubmed publisher
Young K, MacLean S, Dudani R, Krishnan L, Sad S. CD8+ T cells primed in the periphery provide time-bound immune-surveillance to the central nervous system. J Immunol. 2011;187:1192-200 pubmed publisher
Silva Filho J, Souza M, Henriques M, Morrot A, Savino W, Nunes M, et al. AT1 receptor-mediated angiotensin II activation and chemotaxis of T lymphocytes. Mol Immunol. 2011;48:1835-43 pubmed publisher
Uchida Y, Kawai K, Ibusuki A, Kanekura T. Role for E-cadherin as an inhibitory receptor on epidermal gammadelta T cells. J Immunol. 2011;186:6945-54 pubmed publisher
Jang E, Cho W, Cho M, Park H, Oh H, Kang S, et al. Foxp3+ regulatory T cells control humoral autoimmunity by suppressing the development of long-lived plasma cells. J Immunol. 2011;186:1546-53 pubmed publisher
Welch J, Klco J, Varghese N, Nagarajan R, Ley T. Rara haploinsufficiency modestly influences the phenotype of acute promyelocytic leukemia in mice. Blood. 2011;117:2460-8 pubmed publisher
Racine R, McLaughlin M, Jones D, Wittmer S, MacNamara K, Woodland D, et al. IgM production by bone marrow plasmablasts contributes to long-term protection against intracellular bacterial infection. J Immunol. 2011;186:1011-21 pubmed publisher
Chopin M, Quemeneur L, Ripich T, Jessberger R. SWAP-70 controls formation of the splenic marginal zone through regulating T1B-cell differentiation. Eur J Immunol. 2010;40:3544-56 pubmed publisher
Cheung D, Ehlenbach S, Kitchens R, Riley D, Thomas L, Holtzman M, et al. Cutting edge: CD49d+ neutrophils induce FcepsilonRI expression on lung dendritic cells in a mouse model of postviral asthma. J Immunol. 2010;185:4983-7 pubmed publisher
Ryan G, Wang C, Chamberlain J, Attridge K, Schmidt E, Kenefeck R, et al. B1 cells promote pancreas infiltration by autoreactive T cells. J Immunol. 2010;185:2800-7 pubmed publisher
Fulton R, Meyerholz D, Varga S. Foxp3+ CD4 regulatory T cells limit pulmonary immunopathology by modulating the CD8 T cell response during respiratory syncytial virus infection. J Immunol. 2010;185:2382-92 pubmed publisher
Chen L, Lin S, Amin S, Overbergh L, Maggiolino G, Chan L. VCAM-1 blockade delays disease onset, reduces disease severity and inflammatory cells in an atopic dermatitis model. Immunol Cell Biol. 2010;88:334-42 pubmed publisher
Lin K, Tan P, Freeman S, Lam M, McNagny K, Gold M. The Rap GTPases regulate the migration, invasiveness and in vivo dissemination of B-cell lymphomas. Oncogene. 2010;29:608-15 pubmed publisher
Papathanasiou P, Attema J, Karsunky H, Xu J, Smale S, Weissman I. Evaluation of the long-term reconstituting subset of hematopoietic stem cells with CD150. Stem Cells. 2009;27:2498-508 pubmed publisher
Hsu D, Chernyavsky A, Chen H, Yu L, Grando S, Liu F. Endogenous galectin-3 is localized in membrane lipid rafts and regulates migration of dendritic cells. J Invest Dermatol. 2009;129:573-83 pubmed publisher
Brinkman C, Sheasley O neill S, Ferguson A, Engelhard V. Activated CD8 T cells redistribute to antigen-free lymph nodes and exhibit effector and memory characteristics. J Immunol. 2008;181:1814-24 pubmed
Racine R, Chatterjee M, Winslow G. CD11c expression identifies a population of extrafollicular antigen-specific splenic plasmablasts responsible for CD4 T-independent antibody responses during intracellular bacterial infection. J Immunol. 2008;181:1375-85 pubmed
Zhao J, Qi Q, Yang Y, Gu H, Lu N, Liu W, et al. Inhibition of alpha(4) integrin mediated adhesion was involved in the reduction of B16-F10 melanoma cells lung colonization in C57BL/6 mice treated with gambogic acid. Eur J Pharmacol. 2008;589:127-31 pubmed publisher
Kim T, Terwey T, Zakrzewski J, Suh D, Kochman A, Chen M, et al. Organ-derived dendritic cells have differential effects on alloreactive T cells. Blood. 2008;111:2929-40 pubmed publisher
Matsutani T, Tanaka T, Tohya K, Otani K, Jang M, Umemoto E, et al. Plasmacytoid dendritic cells employ multiple cell adhesion molecules sequentially to interact with high endothelial venule cells--molecular basis of their trafficking to lymph nodes. Int Immunol. 2007;19:1031-7 pubmed
Guarda G, Hons M, Soriano S, Huang A, Polley R, MartIn Fontecha A, et al. L-selectin-negative CCR7- effector and memory CD8+ T cells enter reactive lymph nodes and kill dendritic cells. Nat Immunol. 2007;8:743-52 pubmed
Zhu X, Nishimura F, Sasaki K, Fujita M, Dusak J, Eguchi J, et al. Toll like receptor-3 ligand poly-ICLC promotes the efficacy of peripheral vaccinations with tumor antigen-derived peptide epitopes in murine CNS tumor models. J Transl Med. 2007;5:10 pubmed
Ferguson G, Milne L, Kulkarni S, Sasaki T, Walker S, Andrews S, et al. PI(3)Kgamma has an important context-dependent role in neutrophil chemokinesis. Nat Cell Biol. 2007;9:86-91 pubmed
Jin H, Aiyer A, Su J, Borgstrom P, Stupack D, Friedlander M, et al. A homing mechanism for bone marrow-derived progenitor cell recruitment to the neovasculature. J Clin Invest. 2006;116:652-62 pubmed
Gatto D, Pfister T, Jegerlehner A, Martin S, Kopf M, Bachmann M. Complement receptors regulate differentiation of bone marrow plasma cell precursors expressing transcription factors Blimp-1 and XBP-1. J Exp Med. 2005;201:993-1005 pubmed
product information
Product Type :
Antibody
Product Name :
CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), Functional Grade, eBioscience™
Catalog # :
16-0492-85
Quantity :
500µg
Price :
US 323
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Control: Assay-Dependent, Flow Cytometry: 0.5 µg/test, Functional assay: Assay-Dependent
Species :
Mouse
Clone :
R1-2
Isotype :
IgG2b, kappa
Storage :
4° C
Description :
The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.
Format :
Liquid
Applications w/Dilutions :
Control: Assay-Dependent, Flow Cytometry: 0.5 µg/test, Functional assay: Assay-Dependent
Aliases :
269C wild type; alpha 4 subunit of VLA-4 receptor; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; CD49D; IA4; integrin alpha 4; Integrin alpha-4; integrin alpha4B; Integrin alpha-IV; integrin subunit alpha 4; integrin, alpha 4; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); ITGA4; Itga4B; ITGA-IV; LPAM subunit alpha; Lymphocyte Peyer patch adhesion molecules subunit alpha; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA