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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD47 Monoclonal Antibody (B6H12), Functional Grade, eBioscienceâ„¢
catalog :
16-0479-85
quantity :
500µg
price :
US 390
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
B6H12
reactivity :
human, mouse
application :
flow cytometry, blocking or activating experiments
more info or order :
Invitrogen product webpage
citations: 11
Published Application/Species/Sample/DilutionReference
  • blocking or activating experiments; mouse; 20 ug/ml
Reed Geaghan E, Savage J, Hise A, Landreth G. CD14 and toll-like receptors 2 and 4 are required for fibrillar A{beta}-stimulated microglial activation. J Neurosci. 2009;29:11982-92 pubmed publisher
Mair B, Aldridge P, Atwal R, Philpott D, Zhang M, Masud S, et al. High-throughput genome-wide phenotypic screening via immunomagnetic cell sorting. Nat Biomed Eng. 2019;3:796-805 pubmed publisher
Logtenberg M, Jansen J, Raaben M, Toebes M, Franke K, Brandsma A, et al. Glutaminyl cyclase is an enzymatic modifier of the CD47- SIRPα axis and a target for cancer immunotherapy. Nat Med. 2019;25:612-619 pubmed publisher
Guillon J, Petit C, Moreau M, Toutain B, Henry C, Roche H, et al. Regulation of senescence escape by TSP1 and CD47 following chemotherapy treatment. Cell Death Dis. 2019;10:199 pubmed publisher
Hain A, Krämer M, Linka R, Nakhaei Rad S, Ahmadian M, Häussinger D, et al. IL-2 Inducible Kinase ITK is Critical for HIV-1 Infection of Jurkat T-cells. Sci Rep. 2018;8:3217 pubmed publisher
Chen J, Zhong M, Guo H, Davidson D, Mishel S, Lu Y, et al. SLAMF7 is critical for phagocytosis of haematopoietic tumour cells via Mac-1 integrin. Nature. 2017;544:493-497 pubmed publisher
Liu R, Wei H, Gao P, Yu H, Wang K, Fu Z, et al. CD47 promotes ovarian cancer progression by inhibiting macrophage phagocytosis. Oncotarget. 2017;8:39021-39032 pubmed publisher
Kaur S, Chang T, Singh S, Lim L, Mannan P, Garfield S, et al. CD47 signaling regulates the immunosuppressive activity of VEGF in T cells. J Immunol. 2014;193:3914-24 pubmed publisher
Koido S, Homma S, Okamoto M, Namiki Y, Takakura K, Takahara A, et al. Augmentation of antitumor immunity by fusions of ethanol-treated tumor cells and dendritic cells stimulated via dual TLRs through TGF-?1 blockade and IL-12p70 production. PLoS ONE. 2013;8:e63498 pubmed publisher
Bandyopadhyay G, Bankey P, Miller Graziano C. Trauma patients' elevated tumor necrosis related apoptosis inducing ligand (TRAIL) contributes to increased T cell apoptosis. Clin Immunol. 2012;145:44-54 pubmed publisher
Abreu M, Sealy L. Cells expressing the C/EBPbeta isoform, LIP, engulf their neighbors. PLoS ONE. 2012;7:e41807 pubmed publisher
product information
Product Type :
Antibody
Product Name :
CD47 Monoclonal Antibody (B6H12), Functional Grade, eBioscienceâ„¢
Catalog # :
16-0479-85
Quantity :
500µg
Price :
US 390
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 1 µg/test, Functional assay: Assay-Dependent
Species :
Human
Clone :
B6H12
Isotype :
IgG1, kappa
Storage :
4° C
Description :
CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1 µg/test, Functional assay: Assay-Dependent
Aliases :
9130415E20Rik; AA407862; AI848868; antigen identified by monoclonal antibody 1D8; Antigenic surface determinant protein OA3; AW108519; B430305P08Rik; Cd47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; CD47 molecule; CD47/IAP; IAP; integrin associated protein; Integrin-associated protein; integrin-associated signal transducer; Itgp; Leukocyte surface antigen CD47; MER6; OA3; Protein MER6; Rh-related antigen; sCD47; soluble CD 47; soluble CD47
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA