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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD28 Monoclonal Antibody (CD28.2), Functional Grade, eBioscience™
catalog :
16-0289-025
quantity :
25 mg
price :
US 7500.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
CD28.2
reactivity :
human
application :
flow cytometry
more info or order :
citations: 68
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 4a
Liu G, Yu Y, Feng F, Zhu P, Zhang H, Zhang D, et al. Human CD8+CD28- T suppressor cells expanded by common gamma chain (γc) cytokines retain steady allospecific suppressive capacity in vivo. BMC Immunol. 2020;21:23 pubmed publisher
Evans R, Antonelou M, Sathiananthamoorthy S, Rega M, Henderson S, Ceron Gutierrez L, et al. Inherited salt-losing tubulopathies are associated with immunodeficiency due to impaired IL-17 responses. Nat Commun. 2020;11:4368 pubmed publisher
Ferrara M, Chialli G, Ferreira L, Ruggieri E, Careccia G, Preti A, et al. Oxidation of HMGB1 Is a Dynamically Regulated Process in Physiological and Pathological Conditions. Front Immunol. 2020;11:1122 pubmed publisher
Hu J, Lei B, Wen D, Ma B, Zhang T, Lu Z, et al. IL-2 enhanced MHC class I expression in papillary thyroid cancer with Hashimoto's thyroiditis overcomes immune escape in vitro. J Cancer. 2020;11:4250-4260 pubmed publisher
Lu Z, Hu J, Liu W, Wen D, Wei W, Wang Y, et al. IL-10 Restores MHC Class I Expression and Interferes With Immunity in Papillary Thyroid Cancer With Hashimoto Thyroiditis. Endocrinology. 2020;161: pubmed publisher
Futami M, Suzuki K, Kato S, Ohmae S, Tahara Y, Nojima M, et al. The novel multi-cytokine inhibitor TO-207 specifically inhibits pro-inflammatory cytokine secretion in monocytes without affecting the killing ability of CAR T cells. PLoS ONE. 2020;15:e0231896 pubmed publisher
Ma Y, Yang Q, Fan M, Zhang L, Gu Y, Jia W, et al. Placental endovascular extravillous trophoblasts (enEVTs) educate maternal T-cell differentiation along the maternal-placental circulation. Cell Prolif. 2020;53:e12802 pubmed publisher
Tanaskovic O, Verga Falzacappa M, Pelicci P. Human cord blood (hCB)-CD34+ humanized mice fail to reject human acute myeloid leukemia cells. PLoS ONE. 2019;14:e0217345 pubmed publisher
Han L, Hu J, Ma B, Wen D, Zhang T, Lu Z, et al. IL-17A increases MHC class I expression and promotes T cell activation in papillary thyroid cancer patients with coexistent Hashimoto's thyroiditis. Diagn Pathol. 2019;14:52 pubmed publisher
Opstelten R, Slot M, Lardy N, Lankester A, Mulder A, Claas F, et al. Determining the extent of maternal-foetal chimerism in cord blood. Sci Rep. 2019;9:5247 pubmed publisher
Tran H, Herz C, Ruf P, Stetter R, Lamy E. Human T2R38 Bitter Taste Receptor Expression in Resting and Activated Lymphocytes. Front Immunol. 2018;9:2949 pubmed publisher
Ding J, Zhou T, Zheng X, Wang X, Tong X, Luo C, et al. The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis. Acta Cardiol Sin. 2018;34:399-408 pubmed publisher
Idelson M, Alper R, Obolensky A, Yachimovich Cohen N, Rachmilewitz J, Ejzenberg A, et al. Immunological Properties of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells. Stem Cell Reports. 2018;11:681-695 pubmed publisher
Cuadrado E, van den Biggelaar M, de Kivit S, Chen Y, Slot M, Doubal I, et al. Proteomic Analyses of Human Regulatory T Cells Reveal Adaptations in Signaling Pathways that Protect Cellular Identity. Immunity. 2018;48:1046-1059.e6 pubmed publisher
Compeer E, Kraus F, Ecker M, Redpath G, Amiezer M, Rother N, et al. A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation. Nat Commun. 2018;9:1597 pubmed publisher
Shi Y, Zhang P, Wang G, Liu X, Sun X, Zhang X, et al. Description of organ-specific phenotype, and functional characteristics of tissue resident lymphocytes from liver transplantation donor and research on immune tolerance mechanism of liver. Oncotarget. 2018;9:15552-15565 pubmed publisher
Meng Y, Zhou W, Jin L, Liu L, Chang K, Mei J, et al. RANKL-mediated harmonious dialogue between fetus and mother guarantees smooth gestation by inducing decidual M2 macrophage polarization. Cell Death Dis. 2017;8:e3105 pubmed publisher
Hayashi T, Jean M, Huang H, Simpson S, Santoso N, Zhu J. Screening of an FDA-approved compound library identifies levosimendan as a novel anti-HIV-1 agent that inhibits viral transcription. Antiviral Res. 2017;146:76-85 pubmed publisher
Huang H, Liu S, Jean M, Simpson S, Huang H, Merkley M, et al. A Novel Bromodomain Inhibitor Reverses HIV-1 Latency through Specific Binding with BRD4 to Promote Tat and P-TEFb Association. Front Microbiol. 2017;8:1035 pubmed publisher
Chevrier S, Levine J, Zanotelli V, Silina K, Schulz D, Bacac M, et al. An Immune Atlas of Clear Cell Renal Cell Carcinoma. Cell. 2017;169:736-749.e18 pubmed publisher
Datlinger P, Rendeiro A, Schmidl C, Krausgruber T, Traxler P, Klughammer J, et al. Pooled CRISPR screening with single-cell transcriptome readout. Nat Methods. 2017;14:297-301 pubmed publisher
Cárcamo Orive I, Hoffman G, Cundiff P, Beckmann N, D Souza S, Knowles J, et al. Analysis of Transcriptional Variability in a Large Human iPSC Library Reveals Genetic and Non-genetic Determinants of Heterogeneity. Cell Stem Cell. 2017;20:518-532.e9 pubmed publisher
Zhu H, Hu F, Sun X, Zhang X, Zhu L, Liu X, et al. CD16+ Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus. Front Immunol. 2016;7:512 pubmed
Pageon S, Nicovich P, Mollazade M, Tabarin T, Gaus K. Clus-DoC: a combined cluster detection and colocalization analysis for single-molecule localization microscopy data. Mol Biol Cell. 2016;27:3627-3636 pubmed
Pageon S, Tabarin T, Yamamoto Y, Ma Y, Nicovich P, Bridgeman J, et al. Functional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination. Proc Natl Acad Sci U S A. 2016;113:E5454-63 pubmed publisher
Chen H, Händel N, Ngeow J, Muller J, Huhn M, Yang H, et al. Immune dysregulation in patients with PTEN hamartoma tumor syndrome: Analysis of FOXP3 regulatory T cells. J Allergy Clin Immunol. 2017;139:607-620.e15 pubmed publisher
Tang Z, Hao Y, Zhang E, Xu C, Zhou Y, Zheng X, et al. CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade. Mol Med Rep. 2016;14:1107-16 pubmed publisher
Jones R, Mueller S, O Connor R, Rimpel K, Sloan D, Karel D, et al. A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes. PLoS Pathog. 2016;12:e1005545 pubmed publisher
Kapoor Kaushik N, Hinde E, Compeer E, Yamamoto Y, Kraus F, Yang Z, et al. Distinct Mechanisms Regulate Lck Spatial Organization in Activated T Cells. Front Immunol. 2016;7:83 pubmed publisher
Hu H, Wang H, Xiao Y, Jin J, Chang J, Zou Q, et al. Otud7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination. J Exp Med. 2016;213:399-414 pubmed publisher
Soh K, Tario J, Colligan S, Maguire O, Pan D, Minderman H, et al. Simultaneous, Single-Cell Measurement of Messenger RNA, Cell Surface Proteins, and Intracellular Proteins. Curr Protoc Cytom. 2016;75:7.45.1-7.45.33 pubmed publisher
Liu C, Jiang J, Gao L, Wang X, Hu X, Wu M, et al. Soluble PD-1 aggravates progression of collagen-induced arthritis through Th1 and Th17 pathways. Arthritis Res Ther. 2015;17:340 pubmed publisher
Silacci M, Lembke W, Woods R, Attinger Toller I, Baenziger Tobler N, Batey S, et al. Discovery and characterization of COVA322, a clinical-stage bispecific TNF/IL-17A inhibitor for the treatment of inflammatory diseases. MAbs. 2016;8:141-9 pubmed publisher
Hsu P, Santner Nanan B, Hu M, Skarratt K, Lee C, Stormon M, et al. IL-10 Potentiates Differentiation of Human Induced Regulatory T Cells via STAT3 and Foxo1. J Immunol. 2015;195:3665-74 pubmed publisher
Elkord E, Abd Al Samid M, Chaudhary B. Helios, and not FoxP3, is the marker of activated Tregs expressing GARP/LAP. Oncotarget. 2015;6:20026-36 pubmed
Choi J, Goh G, Walradt T, Hong B, Bunick C, Chen K, et al. Genomic landscape of cutaneous T cell lymphoma. Nat Genet. 2015;47:1011-9 pubmed publisher
Chauhan A, Chen C, Moore T, DiPaolo R. Induced expression of FcγRIIIa (CD16a) on CD4+ T cells triggers generation of IFN-γhigh subset. J Biol Chem. 2015;290:5127-40 pubmed publisher
Kovacsovics Bankowski M, Chisholm L, Vercellini J, Tucker C, Montler R, Haley D, et al. Detailed characterization of tumor infiltrating lymphocytes in two distinct human solid malignancies show phenotypic similarities. J Immunother Cancer. 2014;2:38 pubmed publisher
Mellergaard M, Skovbakke S, Schneider C, Lauridsen F, Andresen L, Jensen H, et al. N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24. J Biol Chem. 2014;289:20078-91 pubmed publisher
Kang J, Wang P, Hwang P. Cell-based measurements of mitochondrial function in human subjects. Methods Enzymol. 2014;542:209-21 pubmed publisher
Hartmann N, Giese N, Giese T, Poschke I, Offringa R, Werner J, et al. Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer. Clin Cancer Res. 2014;20:3422-33 pubmed publisher
Wang D, Feng X, Lu L, Konkel J, Zhang H, Chen Z, et al. A CD8 T cell/indoleamine 2,3-dioxygenase axis is required for mesenchymal stem cell suppression of human systemic lupus erythematosus. Arthritis Rheumatol. 2014;66:2234-45 pubmed publisher
Pankratz S, Bittner S, Herrmann A, Schuhmann M, Ruck T, Meuth S, et al. Human CD4+ HLA-G+ regulatory T cells are potent suppressors of graft-versus-host disease in vivo. FASEB J. 2014;28:3435-45 pubmed publisher
Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, et al. Tumor-activated TCR??? T cells from gastric cancer patients induce the antitumor immune response of TCR??? T cells via their antigen-presenting cell-like effects. J Immunol Res. 2014;2014:593562 pubmed publisher
Guo Y, Riley K, Iwasaki A, Steitz J. Alternative capture of noncoding RNAs or protein-coding genes by herpesviruses to alter host T cell function. Mol Cell. 2014;54:67-79 pubmed publisher
Wertheimer A, Bennett M, Park B, Uhrlaub J, Martinez C, Pulko V, et al. Aging and cytomegalovirus infection differentially and jointly affect distinct circulating T cell subsets in humans. J Immunol. 2014;192:2143-55 pubmed publisher
McDonald G, Deepak S, Miguel L, Hall C, Isenberg D, Magee A, et al. Normalizing glycosphingolipids restores function in CD4+ T cells from lupus patients. J Clin Invest. 2014;124:712-24 pubmed publisher
Hellinger R, Koehbach J, Fedchuk H, Sauer B, Huber R, Gruber C, et al. Immunosuppressive activity of an aqueous Viola tricolor herbal extract. J Ethnopharmacol. 2014;151:299-306 pubmed publisher
Pham V, Nakayama M, Itoh Y, Ishigaki H, Kitano M, Arikata M, et al. Pathogenicity of pandemic H1N1 influenza A virus in immunocompromised cynomolgus macaques. PLoS ONE. 2013;8:e75910 pubmed publisher
Koelsch K, Wang Y, Maier Moore J, Sawalha A, Wren J. GFP affects human T cell activation and cytokine production following in vitro stimulation. PLoS ONE. 2013;8:e50068 pubmed publisher
Chicaybam L, Sodré A, Curzio B, Bonamino M. An efficient low cost method for gene transfer to T lymphocytes. PLoS ONE. 2013;8:e60298 pubmed publisher
Miller T, Kaur S, Ivins O Keefe K, Roberts D. Thrombospondin-1 is a CD47-dependent endogenous inhibitor of hydrogen sulfide signaling in T cell activation. Matrix Biol. 2013;32:316-24 pubmed publisher
Palin A, Ramachandran V, Acharya S, Lewis D. Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a. J Immunol. 2013;190:2682-91 pubmed publisher
Li M, Cao W, Liu H, Zhang W, Liu X, Cai Z, et al. MCPIP1 down-regulates IL-2 expression through an ARE-independent pathway. PLoS ONE. 2012;7:e49841 pubmed publisher
Qi Y, Operario D, Georas S, Mosmann T. The acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin. PLoS ONE. 2012;7:e39072 pubmed publisher
Phadwal K, Alegre Abarrategui J, Watson A, Pike L, Anbalagan S, Hammond E, et al. A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells. Autophagy. 2012;8:677-89 pubmed publisher
Ioannou M, Alissafi T, Lazaridis I, Deraos G, Matsoukas J, Gravanis A, et al. Crucial role of granulocytic myeloid-derived suppressor cells in the regulation of central nervous system autoimmune disease. J Immunol. 2012;188:1136-46 pubmed publisher
Miller T, Wang E, Gould S, Stein E, Kaur S, Lim L, et al. Hydrogen sulfide is an endogenous potentiator of T cell activation. J Biol Chem. 2012;287:4211-21 pubmed publisher
Sakane N, Kwon H, Pagans S, Kaehlcke K, Mizusawa Y, Kamada M, et al. Activation of HIV transcription by the viral Tat protein requires a demethylation step mediated by lysine-specific demethylase 1 (LSD1/KDM1). PLoS Pathog. 2011;7:e1002184 pubmed publisher
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Tario J, Muirhead K, Pan D, Munson M, Wallace P. Tracking immune cell proliferation and cytotoxic potential using flow cytometry. Methods Mol Biol. 2011;699:119-64 pubmed publisher
Pan W, Zhu S, Yuan M, Cui H, Wang L, Luo X, et al. MicroRNA-21 and microRNA-148a contribute to DNA hypomethylation in lupus CD4+ T cells by directly and indirectly targeting DNA methyltransferase 1. J Immunol. 2010;184:6773-81 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
CD28 Monoclonal Antibody (CD28.2), Functional Grade, eBioscience™
Catalog # :
16-0289-025
Quantity :
25 mg
Price :
US 7500.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 1 µg/test, Functional assay: Assay-Dependent
Species :
Human
Clone :
CD28.2
Isotype :
IgG1, kappa
Storage :
4° C
Description :
CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1 µg/test, Functional assay: Assay-Dependent
Aliases :
antigen CD28; CD28; CD28 antigen; CD28 antigen (Tp44); CD28 isoform; CD28 isoform 2; Cd28 molecule; CD28 precursor protein; CD28 protein; CD28 protein precursor; CD28RNA; cell surface protein; CHT28; contains partial extracellular domain; costimulatory molecule B7 receptor CD28; EGK_04709; MGC138290; sCD28; soluble CD28; T44; T-cell costimulatory molecule CD28; T-cell costimulatory molecule Tp44; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28 homolog; TP44
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA