CD28 Monoclonal Antibody (CD28.6), Functional Grade, eBioscience™ | Invitrogen 16-0288-81 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD28 Monoclonal Antibody (CD28.6), Functional Grade, eBioscience™

catalog :

16-0288-81

quantity :

50 µg

price :

117 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CD28.6

reactivity :

human

application :

western blot, immunocytochemistry, neutralization, flow cytometry, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 23

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; tbl 1	Villanueva Cabello T, Martinez Duncker I. Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy. J Vis Exp. 2016;: pubmed publisher
neutralization; human	Bashour K, Gondarenko A, Chen H, Shen K, Liu X, Huse M, et al. CD28 and CD3 have complementary roles in T-cell traction forces. Proc Natl Acad Sci U S A. 2014;111:2241-6 pubmed publisher
blocking or activating experiments; human; 15 ug/ml	Quiroga M, Pasquinelli V, Martinez G, Jurado J, Zorrilla L, Musella R, et al. Inducible costimulator: a modulator of IFN-gamma production in human tuberculosis. J Immunol. 2006;176:5965-74 pubmed
	Boutboul D, Kuehn H, Van de Wyngaert Z, Niemela J, Callebaut I, Stoddard J, et al. Dominant-negative IKZF1 mutations cause a T, B, and myeloid cell combined immunodeficiency. J Clin Invest. 2018;128:3071-3087 pubmed publisher
	Chowdhury K, Kumar U, Das S, Chaudhuri J, Kumar P, Kanjilal M, et al. Synovial IL-9 facilitates neutrophil survival, function and differentiation of Th17 cells in rheumatoid arthritis. Arthritis Res Ther. 2018;20:18 pubmed publisher
	Spiesberger K, Paulfranz F, Egger A, Reiser J, Vogl C, Rudolf Scholik J, et al. Large-Scale Purification of r28M: A Bispecific scFv Antibody Targeting Human Melanoma Produced in Transgenic Cattle. PLoS ONE. 2015;10:e0140471 pubmed publisher
	Leone P, Berardi S, Frassanito M, Ria R, De Re V, Cicco S, et al. Dendritic cells accumulate in the bone marrow of myeloma patients where they protect tumor plasma cells from CD8+ T-cell killing. Blood. 2015;126:1443-51 pubmed publisher
	Goettel J, Biswas S, Lexmond W, Yeste A, Passerini L, Patel B, et al. Fatal autoimmunity in mice reconstituted with human hematopoietic stem cells encoding defective FOXP3. Blood. 2015;125:3886-95 pubmed publisher
	Amarnath S, Foley J, Farthing D, Gress R, Laurence A, Eckhaus M, et al. Bone marrow-derived mesenchymal stromal cells harness purinergenic signaling to tolerize human Th1 cells in vivo. Stem Cells. 2015;33:1200-12 pubmed publisher
	Kshirsagar S, Riedl M, Billing H, TÃ¶nshoff B, Thangavadivel S, Steuber C, et al. Akt-dependent enhanced migratory capacity of Th17 cells from children with lupus nephritis. J Immunol. 2014;193:4895-903 pubmed publisher
	Karimi M, Aguilar O, Zou B, Bachmann M, Carlyle J, Baldwin C, et al. A truncated human NKG2D splice isoform negatively regulates NKG2D-mediated function. J Immunol. 2014;193:2764-2771 pubmed publisher
	Chen Z, Wang S, Erekosima N, Li Y, Hong J, Qi X, et al. IL-4 confers resistance to IL-27-mediated suppression on CD4+ T cells by impairing signal transducer and activator of transcription 1 signaling. J Allergy Clin Immunol. 2013;132:912-21.e1-5 pubmed publisher
	Hsu H, Kuan Y, Lin T, Tsao S, Hsu J, Ma L, et al. Reishi Protein LZ-8 Induces FOXP3(+) Treg Expansion via a CD45-Dependent Signaling Pathway and Alleviates Acute Intestinal Inflammation in Mice. Evid Based Complement Alternat Med. 2013;2013:513542 pubmed publisher
	Hasegawa M, Fujimoto M, Matsushita T, Hamaguchi Y, Takehara K. Augmented ICOS expression in patients with early diffuse cutaneous systemic sclerosis. Rheumatology (Oxford). 2013;52:242-51 pubmed publisher
	Tacke R, Lee H, Goh C, Courtney J, Polyak S, Rosen H, et al. Myeloid suppressor cells induced by hepatitis C virus suppress T-cell responses through the production of reactive oxygen species. Hepatology. 2012;55:343-53 pubmed publisher
	Wei J, Wu A, Kong L, Wang Y, Fuller G, Fokt I, et al. Hypoxia potentiates glioma-mediated immunosuppression. PLoS ONE. 2011;6:e16195 pubmed publisher
	Wu A, Wei J, Kong L, Wang Y, Priebe W, Qiao W, et al. Glioma cancer stem cells induce immunosuppressive macrophages/microglia. Neuro Oncol. 2010;12:1113-25 pubmed publisher
	Wei J, Barr J, Kong L, Wang Y, Wu A, Sharma A, et al. Glioblastoma cancer-initiating cells inhibit T-cell proliferation and effector responses by the signal transducers and activators of transcription 3 pathway. Mol Cancer Ther. 2010;9:67-78 pubmed publisher
	Yang J, Zhang J, Cai Q, Zhao D, Wang J, Guo P, et al. Expression and function of inducible costimulator on peripheral blood T cells in patients with systemic lupus erythematosus. Rheumatology (Oxford). 2005;44:1245-54 pubmed
	Zhang X, Olive D, Devos J, Rebouissou C, Ghiotto Ragueneau M, Ferlin M, et al. Malignant plasma cell lines express a functional CD28 molecule. Leukemia. 1998;12:610-8 pubmed
	Kariv I, Truneh A, Sweet R. Analysis of the site of interaction of CD28 with its counter-receptors CD80 and CD86 and correlation with function. J Immunol. 1996;157:29-38 pubmed
	Nunes J, Klasen S, Ragueneau M, Pavon C, Couez D, Mawas C, et al. CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events. Int Immunol. 1993;5:311-5 pubmed
	Bryan R, Li Y, Lai J, Van M, Rice N, Rich R, et al. Effect of CD28 signal transduction on c-Rel in human peripheral blood T cells. Mol Cell Biol. 1994;14:7933-42 pubmed

product information

Product Type :

Antibody

Product Name :

CD28 Monoclonal Antibody (CD28.6), Functional Grade, eBioscience™

Catalog # :

16-0288-81

Quantity :

50 µg

Price :

117 USD

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 1 µg/test, Functional assay: Assay-Dependent, Neutralization: Assay-Dependent, Western Blot: Assay-Dependent

Species :

Human

Clone :

CD28.6

Isotype :

IgG2a, kappa

Storage :

4° C

Description :

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1 µg/test, Functional assay: Assay-Dependent, Neutralization: Assay-Dependent, Western Blot: Assay-Dependent

Aliases :

antigen CD28; Cd28; CD28 antigen; CD28 antigen (Tp44); CD28 isoform; CD28 isoform 2; CD28 molecule; CD28 precursor protein; CD28 protein; CD28 protein precursor; CD28RNA; cell surface protein; CHT28; contains partial extracellular domain; costimulatory molecule B7 receptor CD28; EGK_04709; MGC138290; sCD28; soluble CD28; T44; T-cell costimulatory molecule CD28; T-cell costimulatory molecule Tp44; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28 homolog; TP44

more info or order :

Invitrogen product webpage