product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD16 Monoclonal Antibody (eBioCB16 (CB16)), Functional Grade, eBioscience™
catalog :
16-0168-85
quantity :
500 µg
price :
US 473
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
eBioCB16 (CB16)
reactivity :
human
application :
flow cytometry
more info or order :
citations: 60
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:25; loading ...
Noz M, Bekkering S, Groh L, Nielen T, Lamfers E, Schlitzer A, et al. Reprogramming of bone marrow myeloid progenitor cells in patients with severe coronary artery disease. elife. 2020;9: pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Brouwer P, Caniels T, van der Straten K, Snitselaar J, Aldon Y, Bangaru S, et al. Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability. Science. 2020;369:643-650 pubmed publisher
  • flow cytometry; human; 1:50; loading ...; fig 1d
Stebegg M, Bignon A, Hill D, Silva Cayetano A, Krueger C, Vanderleyden I, et al. Rejuvenating conventional dendritic cells and T follicular helper cell formation after vaccination. elife. 2020;9: pubmed publisher
  • flow cytometry; human; loading ...; fig s1b, s1c, s1d
Bottermann M, Foss S, Caddy S, Clift D, van Tienen L, Vaysburd M, et al. Complement C4 Prevents Viral Infection through Capsid Inactivation. Cell Host Microbe. 2019;25:617-629.e7 pubmed publisher
  • flow cytometry; human; loading ...; fig 9e
Chennupati V, Veiga D, Maslowski K, Andina N, Tardivel A, Yu E, et al. Ribonuclease inhibitor 1 regulates erythropoiesis by controlling GATA1 translation. J Clin Invest. 2018;128:1597-1614 pubmed publisher
  • flow cytometry; human; fig 2
Yin W, Tong S, Zhang Q, Shao J, Liu Q, Peng H, et al. Functional dichotomy of Vδ2 γδ T cells in chronic hepatitis C virus infections: role in cytotoxicity but not for IFN-γ production. Sci Rep. 2016;6:26296 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Dimitrova M, Zenarruzabeitia O, Borrego F, Simhadri V. CD300c is uniquely expressed on CD56 bright Natural Killer Cells and differs from CD300a upon ligand recognition. Sci Rep. 2016;6:23942 pubmed publisher
  • flow cytometry; human; fig 1
Boltjes A, van Montfoort N, Biesta P, Op den Brouw M, Kwekkeboom J, van der Laan L, et al. Kupffer cells interact with hepatitis B surface antigen in vivo and in vitro, leading to proinflammatory cytokine production and natural killer cell function. J Infect Dis. 2015;211:1268-78 pubmed publisher
  • flow cytometry; human
Chao Y, Kaliaperumal N, Chretien A, Tang S, Lee B, Poidinger M, et al. Human plasmacytoid dendritic cells regulate IFN-α production through activation-induced splicing of IL-18Rα. J Leukoc Biol. 2014;96:1037-46 pubmed publisher
Yi J, Peng F, Zhao J, Gong X. Prognostic Lnc-S100B-2 Affects Cell Apoptosis and Microenvironment of Colorectal Cancer through MLLT10 Signaling. J Oncol. 2022;2022:3565118 pubmed publisher
Cui C, Schoenfelt K, Becker K, Becker L. Isolation of polymorphonuclear neutrophils and monocytes from a single sample of human peripheral blood. STAR Protoc. 2021;2:100845 pubmed publisher
Reyes L, A Sanchez Garcia M, Morrison T, Howden A, Watts E, Arienti S, et al. -------A type I IFN, prothrombotic hyperinflammatory neutrophil signature is distinct for COVID-19 ARDS--. Wellcome Open Res. 2021;6:38 pubmed publisher
Agudelo M, Palus M, Keeffe J, Bianchini F, Svoboda P, Sal xe1 t J, et al. Broad and potent neutralizing human antibodies to tick-borne flaviviruses protect mice from disease. J Exp Med. 2021;218: pubmed publisher
Xue J, Xu L, Zhu H, Bai M, Li X, Zhao Z, et al. CD14+CD16- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK. Arthritis Res Ther. 2020;22:221 pubmed publisher
Veras F, Pontelli M, Silva C, Toller Kawahisa J, de Lima M, Nascimento D, et al. SARS-CoV-2-triggered neutrophil extracellular traps mediate COVID-19 pathology. J Exp Med. 2020;217: pubmed publisher
Antonucci L, Porcu C, Timperi E, Santini S, Iannucci G, Balsano C. Circulating Neutrophils of Nonalcoholic Steatohepatitis Patients Show an Activated Phenotype and Suppress T Lymphocytes Activity. J Immunol Res. 2020;2020:4570219 pubmed publisher
Markman J, Porritt R, Wakita D, Lane M, Martinon D, Noval Rivas M, et al. Loss of testosterone impairs anti-tumor neutrophil function. Nat Commun. 2020;11:1613 pubmed publisher
Catapano M, Vergnano M, Romano M, Mahil S, Choon S, Burden A, et al. IL-36 Promotes Systemic IFN-I Responses in Severe Forms of Psoriasis. J Invest Dermatol. 2020;140:816-826.e3 pubmed publisher
Wang Y, Chen C, Qi J, Wu F, Guan J, Chen Z, et al. Altered PGE2-EP2 is associated with an excessive immune response in HBV-related acute-on-chronic liver failure. J Transl Med. 2019;17:93 pubmed publisher
Violari A, Cotton M, Kuhn L, Schramm D, Paximadis M, Loubser S, et al. A child with perinatal HIV infection and long-term sustained virological control following antiretroviral treatment cessation. Nat Commun. 2019;10:412 pubmed publisher
Lebedeva A, Vorobyeva D, Vagida M, Ivanova O, Felker E, Fitzgerald W, et al. Ex vivo culture of human atherosclerotic plaques: A model to study immune cells in atherogenesis. Atherosclerosis. 2017;267:90-98 pubmed publisher
Chiang C, Chao T, Su Y, Hsu C, Chien C, Chiu K, et al. Metformin-treated cancer cells modulate macrophage polarization through AMPK-NF-?B signaling. Oncotarget. 2017;8:20706-20718 pubmed publisher
Lu X, Chen Q, Rong Y, Yang G, Li C, Xu N, et al. LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells. Nat Commun. 2016;7:12719 pubmed publisher
Choi H, Ha S, Kim H, Ahn S, Kang M, Kim K, et al. The prognostic effects of tumor infiltrating regulatory T cells and myeloid derived suppressor cells assessed by multicolor flow cytometry in gastric cancer patients. Oncotarget. 2016;7:7940-51 pubmed publisher
Wang H, Yu J, Li L. A DNA vaccine encoding mutated HPV58 mE6E7-Fc-GPI fusion antigen and GM-CSF and B7.1. Onco Targets Ther. 2015;8:3067-77 pubmed publisher
Sakthivel P, Grunewald J, Eklund A, Bruder D, Wahlström J. Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution. Clin Exp Immunol. 2016;183:294-306 pubmed publisher
Rönn R, Guibentif C, Moraghebi R, Chaves P, Saxena S, Garcia B, et al. Retinoic acid regulates hematopoietic development from human pluripotent stem cells. Stem Cell Reports. 2015;4:269-81 pubmed publisher
Nunes Cabaço H, Matoso P, Foxall R, Tendeiro R, Pires A, Carvalho T, et al. Thymic HIV-2 infection uncovers posttranscriptional control of viral replication in human thymocytes. J Virol. 2015;89:2201-8 pubmed publisher
Wengerter B, Katakowski J, Rosenberg J, Park C, Almo S, Palliser D, et al. Aptamer-targeted antigen delivery. Mol Ther. 2014;22:1375-1387 pubmed publisher
Duggal N, Beswetherick A, Upton J, Hampson P, Phillips A, Lord J. Depressive symptoms in hip fracture patients are associated with reduced monocyte superoxide production. Exp Gerontol. 2014;54:27-34 pubmed publisher
Kragstrup T, Jalilian B, Hvid M, Kjærgaard A, Østgård R, Schiøttz Christensen B, et al. Decreased plasma levels of soluble CD18 link leukocyte infiltration with disease activity in spondyloarthritis. Arthritis Res Ther. 2014;16:R42 pubmed publisher
Oshansky C, Gartland A, Wong S, Jeevan T, Wang D, Roddam P, et al. Mucosal immune responses predict clinical outcomes during influenza infection independently of age and viral load. Am J Respir Crit Care Med. 2014;189:449-62 pubmed publisher
Zhang Y, Kwak Kim J, Huang D, Hu L, Liao A. Cyclic changes and relationship between peripheral and endometrial NK cells from women with repeated failure after artificial insemination by donor sperm. Am J Reprod Immunol. 2014;71:44-54 pubmed publisher
He X, Liang H, Hong K, Li H, Peng H, Zhao Y, et al. The potential role of CD16+ V?2V?2 T cell-mediated antibody-dependent cell-mediated cytotoxicity in control of HIV type 1 disease. AIDS Res Hum Retroviruses. 2013;29:1562-70 pubmed publisher
Os A, Burgler S, Ribes A, Funderud A, Wang D, Thompson K, et al. Chronic lymphocytic leukemia cells are activated and proliferate in response to specific T helper cells. Cell Rep. 2013;4:566-77 pubmed publisher
Peruzzi G, Femnou L, Gil Krzewska A, Borrego F, Weck J, Krzewski K, et al. Membrane-type 6 matrix metalloproteinase regulates the activation-induced downmodulation of CD16 in human primary NK cells. J Immunol. 2013;191:1883-94 pubmed publisher
Ohira M, Nishida S, Matsuura T, Muraoka I, Tryphonopoulos P, Fan J, et al. Comparative analysis of T-cell depletion method for clinical immunotherapy-anti-hepatitis c effects of natural killer cells via interferon-? production. Transplant Proc. 2013;45:2045-50 pubmed publisher
Schwarzmaier D, Foell D, Weinhage T, Varga G, Däbritz J. Peripheral monocyte functions and activation in patients with quiescent Crohn's disease. PLoS ONE. 2013;8:e62761 pubmed publisher
Lim S, Kim T, Lee J, Sonn C, Kim K, Kim J, et al. Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy. Cancer Res. 2013;73:2598-607 pubmed publisher
Besser M, Shoham T, Harari Steinberg O, Zabari N, Ortenberg R, Yakirevitch A, et al. Development of allogeneic NK cell adoptive transfer therapy in metastatic melanoma patients: in vitro preclinical optimization studies. PLoS ONE. 2013;8:e57922 pubmed publisher
Domaica C, Fuertes M, Uriarte I, Girart M, Sardañons J, Comas D, et al. Human natural killer cell maturation defect supports in vivo CD56(bright) to CD56(dim) lineage development. PLoS ONE. 2012;7:e51677 pubmed publisher
Al ofi E, Coffelt S, Anumba D. Monocyte subpopulations from pre-eclamptic patients are abnormally skewed and exhibit exaggerated responses to Toll-like receptor ligands. PLoS ONE. 2012;7:e42217 pubmed publisher
Fiedler R, Neugebauer F, Ulrich C, Wienke A, Gromann C, Storr M, et al. Randomized controlled pilot study of 2 weeks' treatment with high cutoff membrane for hemodialysis patients with elevated C-reactive protein. Artif Organs. 2012;36:886-93 pubmed publisher
Pham B, Piard Ruster K, Silva R, Gallo A, Esquivel C, Martinez O, et al. Changes in natural killer cell subsets in pediatric liver transplant recipients. Pediatr Transplant. 2012;16:176-82 pubmed publisher
Rudnicka K, Włodarczyk M, Moran A, Rechcinski T, Miszczyk E, Matusiak A, et al. Helicobacter pylori antigens as potential modulators of lymphocytes' cytotoxic activity. Microbiol Immunol. 2012;56:62-75 pubmed publisher
Lu Y, Zeng B, Zhang Y, Xiang W, Hu L, Liao A. Quantitative and functional changes in peripheral natural killer cells in women with reproductive failure after artificial insemination with donor sperm. J Reprod Immunol. 2011;91:83-9 pubmed publisher
Landrigan A, Wong M, Utz P. CpG and non-CpG oligodeoxynucleotides directly costimulate mouse and human CD4+ T cells through a TLR9- and MyD88-independent mechanism. J Immunol. 2011;187:3033-43 pubmed publisher
Moustaki A, Argyropoulos K, Baxevanis C, Papamichail M, Perez S. Effect of the simultaneous administration of glucocorticoids and IL-15 on human NK cell phenotype, proliferation and function. Cancer Immunol Immunother. 2011;60:1683-95 pubmed publisher
Araya N, Takahashi K, Sato T, Nakamura T, Sawa C, Hasegawa D, et al. Fucoidan therapy decreases the proviral load in patients with human T-lymphotropic virus type-1-associated neurological disease. Antivir Ther. 2011;16:89-98 pubmed publisher
Qin H, Lee I, Panagiotopoulos C, Wang X, Chu A, Utz P, et al. Natural killer cells from children with type 1 diabetes have defects in NKG2D-dependent function and signaling. Diabetes. 2011;60:857-66 pubmed publisher
Banerjee S, Lin C, Skinner K, Schiffhauer L, Peacock J, Hicks D, et al. Heat shock protein 27 differentiates tolerogenic macrophages that may support human breast cancer progression. Cancer Res. 2011;71:318-27 pubmed publisher
Allan D, Rybalov B, Awong G, Zuniga Pflucker J, Kopcow H, Carlyle J, et al. TGF-? affects development and differentiation of human natural killer cell subsets. Eur J Immunol. 2010;40:2289-95 pubmed publisher
Bankey P, Banerjee S, Zucchiatti A, De M, Sleem R, Lin C, et al. Cytokine induced expression of programmed death ligands in human neutrophils. Immunol Lett. 2010;129:100-7 pubmed publisher
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Azakami K, Sato T, Araya N, Utsunomiya A, Kubota R, Suzuki K, et al. Severe loss of invariant NKT cells exhibiting anti-HTLV-1 activity in patients with HTLV-1-associated disorders. Blood. 2009;114:3208-15 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
CD16 Monoclonal Antibody (eBioCB16 (CB16)), Functional Grade, eBioscience™
Catalog # :
16-0168-85
Quantity :
500 µg
Price :
US 473
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Control: Assay-Dependent, Flow Cytometry: 0.5 µg/test, Functional assay: Assay-Dependent
Species :
Human
Clone :
eBioCB16 (CB16)
Isotype :
IgG1, kappa
Storage :
4° C
Description :
CD16 (FCGR3A) is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Human CD16 is expressed in two forms – FCGR3A and FCGR3B. FCGR3A is associated with the FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FCGR3A is associated, with FcepsilonRI-beta subunit. Besides IgG, FCGR3A can be triggered also by oligomeric IgE. FCGR3B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a pro-adhesive phenotype. Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.
Format :
Liquid
Applications w/Dilutions :
Control: Assay-Dependent, Flow Cytometry: 0.5 µg/test, Functional assay: Assay-Dependent
Aliases :
4833442P21Rik; CD16; CD16-2; CD16A; CD16a antigen; CD16B; CD32; CD32 receptor 2; CDw32; cytolytic trigger molecule G7; Fc fragment of IgG intermediate affinity IV receptor; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG receptor IIIa; Fc fragment of IgG receptor IIIb; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III; Fc gamma receptor IIIa; Fc gamma receptor III-A; Fc gamma receptor IIIb; Fc gamma RIIIa; Fc gammaRIV; Fc receptor, IgG, low affinity III; Fc receptor, IgG, low affinity IV; Fc receptor-like 3; Fcg receptor III; FCG2; FCG3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RII-b; Fc-gamma RII-c; fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; fc-gamma RIIIb; fc-gamma RIII-beta; Fc-gamma-RIIb; Fc-gamma-RIIc; FcgammaRIII; FcgammaRIII a.1; FcgammaRIII a.2; FcgammaRIII a.3; FcgammaRIIIA; FcgammaRIV; FCGR2; FCGR2A; FCGR2B; FCGR2C; FCGR3; Fcgr3a; FCGR3B; Fcgr4; FCGRIII; FcgRIV; FCR-10; FcRII-b; FcRII-c; FcRIII; FCRIIIA; FCRIIIb; Fcrl3; IGFR3; IgG Fc gamma receptor III; IgG Fc receptor III; igG Fc receptor III-1; IgG Fc receptor III-2; IMD20; immunoglobulin G Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor III; low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; Low affinity immunoglobulin gamma Fc region receptor IV; neutrophil-specific antigen NA; RP11-5K23.1; transmembrane receptor FcgammaRIII-X
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA