CD16 Monoclonal Antibody (3G8), Functional Grade, eBioscience™ | Invitrogen 16-0166-85 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD16 Monoclonal Antibody (3G8), Functional Grade, eBioscience™

catalog :

16-0166-85

quantity :

500 µg

price :

US 497

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3G8

reactivity :

human, rhesus macaque

application :

immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 22

Published Application/Species/Sample/Dilution	Reference
flow cytometry; rhesus macaque; tbl 1	Williams D, Engle E, Shirk E, Queen S, Gama L, Mankowski J, et al. Splenic Damage during SIV Infection: Role of T-Cell Depletion and Macrophage Polarization and Infection. Am J Pathol. 2016;186:2068-2087 pubmed publisher
immunoprecipitation; human	Chauhan A, Chen C, Moore T, DiPaolo R. Induced expression of FcÎ³RIIIa (CD16a) on CD4+ T cells triggers generation of IFN-Î³high subset. J Biol Chem. 2015;290:5127-40 pubmed publisher
flow cytometry; human; fig 8	Babu R, Brown A. A consensus surface activation marker signature is partially dependent on human immunodeficiency virus type 1 Nef expression within productively infected macrophages. Retrovirology. 2013;10:155 pubmed publisher
flow cytometry; human; fig 1, 2, 3	Bratke K, Klein C, Kuepper M, Lommatzsch M, Virchow J. Differential development of plasmacytoid dendritic cells in Th1- and Th2-like cytokine milieus. Allergy. 2011;66:386-95 pubmed publisher
	Singh Y, Trautwein C, Fendel R, Krickeberg N, Berezhnoy G, Bissinger R, et al. SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells. Heliyon. 2021;7:e07147 pubmed publisher
	Warren C, Meyerson N, Dirasantha O, Feldman E, Wilkerson G, Sawyer S. Selective use of primate CD4 receptors by HIV-1. PLoS Biol. 2019;17:e3000304 pubmed publisher
	Blom A, Magda M, Kohl L, Shaughnessy J, Lambris J, Ram S, et al. Factor H-IgG Chimeric Proteins as a Therapeutic Approach against the Gram-Positive Bacterial Pathogen Streptococcus pyogenes. J Immunol. 2017;199:3828-3839 pubmed publisher
	Kyoizumi S, Kubo Y, Kajimura J, Yoshida K, Hayashi T, Nakachi K, et al. Fate Decision Between Group 3 Innate Lymphoid and Conventional NK Cell Lineages by Notch Signaling in Human Circulating Hematopoietic Progenitors. J Immunol. 2017;199:2777-2793 pubmed publisher
	Hasan M, Ryan P, Bergmeier L, Fortune F. Circulating NK cells and their subsets in Behçet's disease. Clin Exp Immunol. 2017;188:311-322 pubmed publisher
	Senbabaoglu Y, Gejman R, Winer A, Liu M, Van Allen E, de Velasco G, et al. Tumor immune microenvironment characterization in clear cell renal cell carcinoma identifies prognostic and immunotherapeutically relevant messenger RNA signatures. Genome Biol. 2016;17:231 pubmed
	Cheeseman H, Olejniczak N, Rogers P, Evans A, King D, Ziprin P, et al. Broadly Neutralizing Antibodies Display Potential for Prevention of HIV-1 Infection of Mucosal Tissue Superior to That of Nonneutralizing Antibodies. J Virol. 2017;91: pubmed publisher
	Wang Y, Ma C, Ling Y, Bousfiha A, Camcioglu Y, Jacquot S, et al. Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations. J Exp Med. 2016;213:2413-2435 pubmed
	Jordan N, Bardia A, Wittner B, Benes C, Ligorio M, Zheng Y, et al. HER2 expression identifies dynamic functional states within circulating breast cancer cells. Nature. 2016;537:102-106 pubmed publisher
	ClÃ©menceau B, Valsesia Wittmann S, Jallas A, Vivien R, Rousseau R, Marabelle A, et al. In Vitro and In Vivo Comparison of Lymphocytes Transduced with a Human CD16 or with a Chimeric Antigen Receptor Reveals Potential Off-Target Interactions due to the IgG2 CH2-CH3 CAR-Spacer. J Immunol Res. 2015;2015:482089 pubmed publisher
	Rosario M, Liu B, Kong L, Collins L, Schneider S, Chen X, et al. The IL-15-Based ALT-803 Complex Enhances FcÎ³RIIIa-Triggered NK Cell Responses and In Vivo Clearance of B Cell Lymphomas. Clin Cancer Res. 2016;22:596-608 pubmed publisher
	Kinder M, Greenplate A, Strohl W, Jordan R, Brezski R. An Fc engineering approach that modulates antibody-dependent cytokine release without altering cell-killing functions. MAbs. 2015;7:494-504 pubmed publisher
	Boltjes A, Groothuismink Z, van Oord G, Janssen H, Woltman A, Boonstra A. Monocytes from chronic HBV patients react in vitro to HBsAg and TLR by producing cytokines irrespective of stage of disease. PLoS ONE. 2014;9:e97006 pubmed publisher
	Watchmaker P, Lahl K, Lee M, Baumjohann D, Morton J, Kim S, et al. Comparative transcriptional and functional profiling defines conserved programs of intestinal DC differentiation in humans and mice. Nat Immunol. 2014;15:98-108 pubmed publisher
	Ming Lim C, Stephenson R, Salazar A, Ferris R. TLR3 agonists improve the immunostimulatory potential of cetuximab against EGFR+ head and neck cancer cells. Oncoimmunology. 2013;2:e24677 pubmed
	Algra S, Groeneveld K, Schadenberg A, Haas F, Evens F, Meerding J, et al. Cerebral ischemia initiates an immediate innate immune response in neonates during cardiac surgery. J Neuroinflammation. 2013;10:24 pubmed publisher
	Seu L, Burt T, Witte J, Martin J, Deeks S, McCune J. Variations in the heme oxygenase-1 microsatellite polymorphism are associated with plasma CD14 and viral load in HIV-infected African-Americans. Genes Immun. 2012;13:258-67 pubmed publisher
	Berard F, Blanco P, Davoust J, Neidhart Berard E, Nouri Shirazi M, Taquet N, et al. Cross-priming of naive CD8 T cells against melanoma antigens using dendritic cells loaded with killed allogeneic melanoma cells. J Exp Med. 2000;192:1535-44 pubmed

product information

Product Type :

Antibody

Product Name :

CD16 Monoclonal Antibody (3G8), Functional Grade, eBioscience™

Catalog # :

16-0166-85

Quantity :

500 µg

Price :

US 497

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Baboon, Chimpanzee, Cynomolgus Monkey, Human, Non-human primate, Rhesus Monkey

Applications :

Flow Cytometry: 1 µg/test, Functional assay: Assay-Dependent

Species :

Baboon, Chimpanzee, Cynomolgus Monkey, Human, Non-human primate, Rhesus Monkey

Clone :

3G8

Isotype :

IgG1

Storage :

4° C

Description :

CD16 (FCGR3A) is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Human CD16 is expressed in two forms – FCGR3A and FCGR3B. FCGR3A is associated with the FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FCGR3A is associated, with FcepsilonRI-beta subunit. Besides IgG, FCGR3A can be triggered also by oligomeric IgE. FCGR3B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a pro-adhesive phenotype. Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1 µg/test, Functional assay: Assay-Dependent

Aliases :

4833442P21Rik; CD16; CD16-2; CD16A; CD16a antigen; CD16B; CD32; CD32 receptor 2; CDw32; cytolytic trigger molecule G7; Fc fragment of IgG intermediate affinity IV receptor; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG receptor IIIa; Fc fragment of IgG receptor IIIb; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III; Fc gamma receptor IIIa; Fc gamma receptor III-A; Fc gamma receptor IIIb; Fc gamma RIIIa; Fc gammaRIV; Fc receptor, IgG, low affinity III; Fc receptor, IgG, low affinity IV; Fc receptor-like 3; Fcg receptor III; FCG2; FCG3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RII-b; Fc-gamma RII-c; fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; fc-gamma RIIIb; fc-gamma RIII-beta; Fc-gamma-RIIb; Fc-gamma-RIIc; FcgammaRIII; FcgammaRIII a.1; FcgammaRIII a.2; FcgammaRIII a.3; FcgammaRIIIA; FcgammaRIV; FCGR2; FCGR2A; FCGR2B; FCGR2C; FCGR3; Fcgr3a; FCGR3B; Fcgr4; FCGRIII; FcgRIV; FCR-10; FcRII-b; FcRII-c; FcRIII; FCRIIIA; FCRIIIb; Fcrl3; IGFR3; IgG Fc gamma receptor III; IgG Fc receptor III; igG Fc receptor III-1; IgG Fc receptor III-2; IMD20; immunoglobulin G Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor III; low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; Low affinity immunoglobulin gamma Fc region receptor IV; neutrophil-specific antigen NA; RP11-5K23.1; transmembrane receptor FcgammaRIII-X

more info or order :

Invitrogen product webpage