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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD16/CD32 Monoclonal Antibody (93), Functional Grade, eBioscience™
catalog :
16-0161-82
quantity :
100 µg
price :
US 179
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
93
reactivity :
human, mouse
application :
immunocytochemistry, neutralization, flow cytometry, blocking or activating experiments
more info or order :
citations: 70
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s1e
Baumgartner C, Toifl S, Farlik M, Halbritter F, Scheicher R, Fischer I, et al. An ERK-Dependent Feedback Mechanism Prevents Hematopoietic Stem Cell Exhaustion. Cell Stem Cell. 2018;22:879-892.e6 pubmed publisher
  • blocking or activating experiments; mouse; loading ...; fig s3c
Cordova Z, Grönholm A, Kytölä V, Taverniti V, Hämäläinen S, Aittomäki S, et al. Myeloid cell expressed proprotein convertase FURIN attenuates inflammation. Oncotarget. 2016;7:54392-54404 pubmed publisher
  • blocking or activating experiments; mouse
  • flow cytometry; mouse
Foy S, Sennino B, dela Cruz T, Cote J, Gordon E, Kemp F, et al. Poxvirus-Based Active Immunotherapy with PD-1 and LAG-3 Dual Immune Checkpoint Inhibition Overcomes Compensatory Immune Regulation, Yielding Complete Tumor Regression in Mice. PLoS ONE. 2016;11:e0150084 pubmed publisher
  • flow cytometry; mouse
Chen J, Miyanishi M, Wang S, Yamazaki S, Sinha R, Kao K, et al. Hoxb5 marks long-term haematopoietic stem cells and reveals a homogenous perivascular niche. Nature. 2016;530:223-7 pubmed publisher
  • flow cytometry; mouse; 1:400; fig s4
Ludigs K, Jandus C, Utzschneider D, Staehli F, Bessoles S, Dang A, et al. NLRC5 shields T lymphocytes from NK-cell-mediated elimination under inflammatory conditions. Nat Commun. 2016;7:10554 pubmed publisher
  • flow cytometry; mouse; fig 5, 6
Sagoo P, Garcia Z, Breart B, Lemaître F, Michonneau D, Albert M, et al. In vivo imaging of inflammasome activation reveals a subcapsular macrophage burst response that mobilizes innate and adaptive immunity. Nat Med. 2016;22:64-71 pubmed publisher
  • blocking or activating experiments; mouse; loading ...
Cole C, Verdoni A, Ketkar S, Leight E, Russler Germain D, Lamprecht T, et al. PML-RARA requires DNA methyltransferase 3A to initiate acute promyelocytic leukemia. J Clin Invest. 2016;126:85-98 pubmed publisher
  • flow cytometry; human; tbl 5
Hayashi Y, Bardsley M, Toyomasu Y, Milosavljevic S, Gajdos G, Choi K, et al. Platelet-Derived Growth Factor Receptor-α Regulates Proliferation of Gastrointestinal Stromal Tumor Cells With Mutations in KIT by Stabilizing ETV1. Gastroenterology. 2015;149:420-32.e16 pubmed publisher
  • flow cytometry; mouse; fig 1
Johnson A, Costanzo A, Gareau M, Armando A, Quehenberger O, Jameson J, et al. High fat diet causes depletion of intestinal eosinophils associated with intestinal permeability. PLoS ONE. 2015;10:e0122195 pubmed publisher
  • flow cytometry; mouse; fig 4
Jobsri J, Allen A, Rajagopal D, Shipton M, Kanyuka K, Lomonossoff G, et al. Plant virus particles carrying tumour antigen activate TLR7 and Induce high levels of protective antibody. PLoS ONE. 2015;10:e0118096 pubmed publisher
  • blocking or activating experiments; mouse; 5 ug/ml
Sell S, Dietz M, Schneider A, Holtappels R, Mach M, Winkler T. Control of murine cytomegalovirus infection by γδ T cells. PLoS Pathog. 2015;11:e1004481 pubmed publisher
  • blocking or activating experiments; mouse; loading ...; fig 2c
Herbst S, Shah A, Mazon Moya M, Marzola V, Jensen B, Reed A, et al. Phagocytosis-dependent activation of a TLR9-BTK-calcineurin-NFAT pathway co-ordinates innate immunity to Aspergillus fumigatus. EMBO Mol Med. 2015;7:240-58 pubmed publisher
  • flow cytometry; human
Hannani D, Vétizou M, Enot D, Rusakiewicz S, Chaput N, Klatzmann D, et al. Anticancer immunotherapy by CTLA-4 blockade: obligatory contribution of IL-2 receptors and negative prognostic impact of soluble CD25. Cell Res. 2015;25:208-24 pubmed publisher
  • blocking or activating experiments; mouse
Teixeira L, Moreira J, Melo J, Bezerra F, Marques R, Ferreirinha P, et al. Immune response in the adipose tissue of lean mice infected with the protozoan parasite Neospora caninum. Immunology. 2015;145:242-57 pubmed publisher
  • flow cytometry; mouse; fig 2
Krysiak K, Tibbitts J, Shao J, Liu T, Ndonwi M, Walter M. Reduced levels of Hspa9 attenuate Stat5 activation in mouse B cells. Exp Hematol. 2015;43:319-30.e10 pubmed publisher
  • flow cytometry; mouse; 1:200
Peschke K, Dudeck A, Rabenhorst A, Hartmann K, Roers A. Cre/loxP-based mouse models of mast cell deficiency and mast cell-specific gene inactivation. Methods Mol Biol. 2015;1220:403-21 pubmed publisher
  • blocking or activating experiments; mouse
Škrnjug I, Guzmán C, Rueckert C, Ruecker C. Cyclic GMP-AMP displays mucosal adjuvant activity in mice. PLoS ONE. 2014;9:e110150 pubmed publisher
  • flow cytometry; mouse
Skrnjug I, Rueckert C, Libanova R, Lienenklaus S, Weiss S, Guzman C. The mucosal adjuvant cyclic di-AMP exerts immune stimulatory effects on dendritic cells and macrophages. PLoS ONE. 2014;9:e95728 pubmed publisher
  • flow cytometry; mouse
Satpathy A, Briseño C, Lee J, Ng D, Manieri N, Kc W, et al. Notch2-dependent classical dendritic cells orchestrate intestinal immunity to attaching-and-effacing bacterial pathogens. Nat Immunol. 2013;14:937-48 pubmed publisher
  • flow cytometry; mouse; fig 5a
Wu X, Satpathy A, Kc W, Liu P, Murphy T, Murphy K. Bcl11a controls Flt3 expression in early hematopoietic progenitors and is required for pDC development in vivo. PLoS ONE. 2013;8:e64800 pubmed publisher
  • flow cytometry; mouse; fig 7
Vink P, Smout W, Driessen Engels L, de Bruin A, Delsing D, Krajnc Franken M, et al. In vivo knockdown of TAK1 accelerates bone marrow proliferation/differentiation and induces systemic inflammation. PLoS ONE. 2013;8:e57348 pubmed publisher
  • flow cytometry; mouse
Ripich T, Jessberger R. SWAP-70 regulates erythropoiesis by controlling ?4 integrin. Haematologica. 2011;96:1743-52 pubmed publisher
  • immunocytochemistry; mouse; 5 ug/ml; fig 5
Weisel F, Appelt U, Schneider A, Horlitz J, Van Rooijen N, Korner H, et al. Unique requirements for reactivation of virus-specific memory B lymphocytes. J Immunol. 2010;185:4011-21 pubmed publisher
  • flow cytometry; mouse; tbl 1
Zavitz C, Bauer C, Gaschler G, Fraser K, Strieter R, Hogaboam C, et al. Dysregulated macrophage-inflammatory protein-2 expression drives illness in bacterial superinfection of influenza. J Immunol. 2010;184:2001-13 pubmed publisher
  • flow cytometry; mouse
Maillard I, Chen Y, Friedman A, Yang Y, Tubbs A, Shestova O, et al. Menin regulates the function of hematopoietic stem cells and lymphoid progenitors. Blood. 2009;113:1661-9 pubmed publisher
  • blocking or activating experiments; mouse
  • flow cytometry; mouse; fig s5
Waskow C, Liu K, Darrasse Jèze G, Guermonprez P, Ginhoux F, Merad M, et al. The receptor tyrosine kinase Flt3 is required for dendritic cell development in peripheral lymphoid tissues. Nat Immunol. 2008;9:676-83 pubmed publisher
  • blocking or activating experiments; mouse
Jiang L, Yang P, He H, Li B, Lin X, Hou S, et al. Increased expression of Foxp3 in splenic CD8+ T cells from mice with anterior chamber-associated immune deviation. Mol Vis. 2007;13:968-74 pubmed
  • flow cytometry; mouse; fig 4
Gupta R, Karpatkin S, Basch R. Hematopoiesis and stem cell renewal in long-term bone marrow cultures containing catalase. Blood. 2006;107:1837-46 pubmed
Berube A, Gmyrek G, Royer D, Carr D. Tripartite-Motif 21 (TRIM21) Deficiency Results in a Modest Loss of Herpes Simplex Virus (HSV)-1 Surveillance in the Trigeminal Ganglia Following Cornea Infection. Viruses. 2022;14: pubmed publisher
Picard M, Yonekura S, Slowicka K, Petta I, Rauber C, Routy B, et al. Ileal immune tonus is a prognosis marker of proximal colon cancer in mice and patients. Cell Death Differ. 2021;28:1532-1547 pubmed publisher
Newman L, Jasim D, Prestat E, Lozano N, de Lázaro I, Nam Y, et al. Splenic Capture and In Vivo Intracellular Biodegradation of Biological-Grade Graphene Oxide Sheets. ACS Nano. 2020;14:10168-10186 pubmed publisher
Agalave N, Lane B, Mody P, Szabo Pardi T, Burton M. Isolation, culture, and downstream characterization of primary microglia and astrocytes from adult rodent brain and spinal cord. J Neurosci Methods. 2020;340:108742 pubmed publisher
Donado C, Cao A, Simmons D, Croker B, Brennan P, Brenner M. A Two-Cell Model for IL-1β Release Mediated by Death-Receptor Signaling. Cell Rep. 2020;31:107466 pubmed publisher
Balog S, Li Y, Ogawa T, Miki T, Saito T, French S, et al. Development of Capsular Fibrosis Beneath the Liver Surface in Humans and Mice. Hepatology. 2020;71:291-305 pubmed publisher
Ershaid N, Sharon Y, Doron H, Raz Y, Shani O, Cohen N, et al. NLRP3 inflammasome in fibroblasts links tissue damage with inflammation in breast cancer progression and metastasis. Nat Commun. 2019;10:4375 pubmed publisher
Luan H, Wang A, Hilliard B, Carvalho F, Rosen C, Ahasic A, et al. GDF15 Is an Inflammation-Induced Central Mediator of Tissue Tolerance. Cell. 2019;178:1231-1244.e11 pubmed publisher
Senserrich J, Batsivari A, Rybtsov S, Gordon Keylock S, Souilhol C, Buchholz F, et al. Analysis of Runx1 Using Induced Gene Ablation Reveals Its Essential Role in Pre-liver HSC Development and Limitations of an In Vivo Approach. Stem Cell Reports. 2018;11:784-794 pubmed publisher
Metidji A, Omenetti S, Crotta S, Li Y, Nye E, Ross E, et al. The Environmental Sensor AHR Protects from Inflammatory Damage by Maintaining Intestinal Stem Cell Homeostasis and Barrier Integrity. Immunity. 2018;49:353-362.e5 pubmed publisher
Lam J, van den Bosch M, Wegrzyn J, Parker J, Ibrahim R, Slowski K, et al. miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling. Nat Commun. 2018;9:2418 pubmed publisher
Youssif C, Cubillos Rojas M, Comalada M, Llonch E, Perna C, Djouder N, et al. Myeloid p38α signaling promotes intestinal IGF-1 production and inflammation-associated tumorigenesis. EMBO Mol Med. 2018;10: pubmed publisher
Clark H, Abbondante S, Minns M, Greenberg E, Sun Y, Pearlman E. Protein Deiminase 4 and CR3 Regulate Aspergillus fumigatus and β-Glucan-Induced Neutrophil Extracellular Trap Formation, but Hyphal Killing Is Dependent Only on CR3. Front Immunol. 2018;9:1182 pubmed publisher
Shourian M, Ralph B, Angers I, Sheppard D, Qureshi S. Contribution of IL-1RI Signaling to Protection against Cryptococcus neoformans 52D in a Mouse Model of Infection. Front Immunol. 2017;8:1987 pubmed publisher
Colinot D, Garbuz T, Bosland M, Wang L, Rice S, Sullivan W, et al. The common parasite Toxoplasma gondii induces prostatic inflammation and microglandular hyperplasia in a mouse model. Prostate. 2017;77:1066-1075 pubmed publisher
Schampel A, Volovitch O, Koeniger T, Scholz C, Jörg S, Linker R, et al. Nimodipine fosters remyelination in a mouse model of multiple sclerosis and induces microglia-specific apoptosis. Proc Natl Acad Sci U S A. 2017;114:E3295-E3304 pubmed publisher
Hérault A, Binnewies M, Leong S, Calero Nieto F, Zhang S, Kang Y, et al. Myeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis. Nature. 2017;544:53-58 pubmed publisher
Liu Z, Ravindranathan R, Kalinski P, Guo Z, Bartlett D. Rational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy. Nat Commun. 2017;8:14754 pubmed publisher
Le Q, Yao W, Chen Y, Yan B, Liu C, Yuan M, et al. GRK6 regulates ROS response and maintains hematopoietic stem cell self-renewal. Cell Death Dis. 2016;7:e2478 pubmed publisher
Wang A, Huen S, Luan H, Yu S, Zhang C, Gallezot J, et al. Opposing Effects of Fasting Metabolism on Tissue Tolerance in Bacterial and Viral Inflammation. Cell. 2016;166:1512-1525.e12 pubmed publisher
Hu D, Yin C, Mohanta S, Weber C, Habenicht A. Preparation of Single Cell Suspensions from Mouse Aorta. Bio Protoc. 2016;6: pubmed
Stentzel S, Teufelberger A, Nordengrün M, Kolata J, Schmidt F, Van Crombruggen K, et al. Staphylococcal serine protease-like proteins are pacemakers of allergic airway reactions to Staphylococcus aureus. J Allergy Clin Immunol. 2017;139:492-500.e8 pubmed publisher
Seehus C, Kaye J. In vitro Differentiation of Murine Innate Lymphoid Cells from Common Lymphoid Progenitor Cells. Bio Protoc. 2016;6: pubmed
O Shea N, Chew T, Dunne J, Marnane R, Nedjat Shokouhi B, Smith P, et al. Critical Role of the Disintegrin Metalloprotease ADAM-like Decysin-1 [ADAMDEC1] for Intestinal Immunity and Inflammation. J Crohns Colitis. 2016;10:1417-1427 pubmed
Patel M, Kim J, Theodros D, Tam A, Velarde E, Kochel C, et al. Agonist anti-GITR monoclonal antibody and stereotactic radiation induce immune-mediated survival advantage in murine intracranial glioma. J Immunother Cancer. 2016;4:28 pubmed publisher
Schlam D, Canton J, Carreño M, Kopinski H, Freeman S, Grinstein S, et al. Gliotoxin Suppresses Macrophage Immune Function by Subverting Phosphatidylinositol 3,4,5-Trisphosphate Homeostasis. MBio. 2016;7:e02242 pubmed publisher
Karmakar M, Katsnelson M, Dubyak G, Pearlman E. Neutrophil P2X7 receptors mediate NLRP3 inflammasome-dependent IL-1β secretion in response to ATP. Nat Commun. 2016;7:10555 pubmed publisher
Lizotte P, Wen A, Sheen M, Fields J, Rojanasopondist P, Steinmetz N, et al. In situ vaccination with cowpea mosaic virus nanoparticles suppresses metastatic cancer. Nat Nanotechnol. 2016;11:295-303 pubmed publisher
Clark H, Jhingran A, Sun Y, Vareechon C, de Jesus Carrion S, Skaar E, et al. Zinc and Manganese Chelation by Neutrophil S100A8/A9 (Calprotectin) Limits Extracellular Aspergillus fumigatus Hyphal Growth and Corneal Infection. J Immunol. 2016;196:336-44 pubmed publisher
Benson M, Manzanero S, Borges K. Complex alterations in microglial M1/M2 markers during the development of epilepsy in two mouse models. Epilepsia. 2015;56:895-905 pubmed publisher
Nakagawa M, Thummar K, Mandelbaum J, Pasqualucci L, RATHINAM C. Lack of the ubiquitin-editing enzyme A20 results in loss of hematopoietic stem cell quiescence. J Exp Med. 2015;212:203-16 pubmed publisher
Llewellyn D, de Cassan S, Williams A, Douglas A, Forbes E, Adame Gallegos J, et al. Assessment of antibody-dependent respiratory burst activity from mouse neutrophils on Plasmodium yoelii malaria challenge outcome. J Leukoc Biol. 2014;95:369-82 pubmed publisher
Silva E, Goodyear A, Sutherland M, Podnecky N, Gonzalez Juarrero M, Schweizer H, et al. Correlates of immune protection following cutaneous immunization with an attenuated Burkholderia pseudomallei vaccine. Infect Immun. 2013;81:4626-34 pubmed publisher
Guirnalda P, Wood L, Goenka R, Crespo J, Paterson Y. Interferon ?-induced intratumoral expression of CXCL9 alters the local distribution of T cells following immunotherapy with Listeria monocytogenes. Oncoimmunology. 2013;2:e25752 pubmed
Kaanta A, Virtanen C, Selfors L, Brugge J, Neel B. Evidence for a multipotent mammary progenitor with pregnancy-specific activity. Breast Cancer Res. 2013;15:R65 pubmed
Pirnes Karhu S, Mäntymaa P, Sironen R, Mäkinen P, Wojciechowski S, Juutinen S, et al. Enhanced polyamine catabolism disturbs hematopoietic lineage commitment and leads to a myeloproliferative disease in mice overexpressing spermidine/spermine N¹-acetyltransferase. Amino Acids. 2014;46:689-700 pubmed publisher
Guirnalda P, Paterson Y. Vaccination with immunotherapeutic Listeria monocytogenes induces IL-17(+) ?? T cells in a murine model for HPV associated cancer. Oncoimmunology. 2012;1:822-828 pubmed
Pello O, Chevre R, Laoui D, De Juan A, Lolo F, Andrés Manzano M, et al. In vivo inhibition of c-MYC in myeloid cells impairs tumor-associated macrophage maturation and pro-tumoral activities. PLoS ONE. 2012;7:e45399 pubmed publisher
Herrmann M, Schafer C, Heiss A, Gräber S, Kinkeldey A, Büscher A, et al. Clearance of fetuin-A--containing calciprotein particles is mediated by scavenger receptor-A. Circ Res. 2012;111:575-84 pubmed publisher
Ninkovic J, Roy S. Morphine decreases bacterial phagocytosis by inhibiting actin polymerization through cAMP-, Rac-1-, and p38 MAPK-dependent mechanisms. Am J Pathol. 2012;180:1068-79 pubmed publisher
Hsiao E, Patterson P. Activation of the maternal immune system induces endocrine changes in the placenta via IL-6. Brain Behav Immun. 2011;25:604-15 pubmed publisher
Buhtoiarov I, Rakhmilevich A, Lanier L, Ranheim E, Sondel P. Naive mouse macrophages become activated following recognition of L5178Y lymphoma cells via concurrent ligation of CD40, NKG2D, and CD18 molecules. J Immunol. 2009;182:1940-53 pubmed publisher
product information
Product Type :
Antibody
Product Name :
CD16/CD32 Monoclonal Antibody (93), Functional Grade, eBioscience™
Catalog # :
16-0161-82
Quantity :
100 µg
Price :
US 179
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.5 µg/test, Functional assay: Assay-Dependent, Neutralization: Assay-Dependent
Species :
Mouse
Clone :
93
Isotype :
IgG2a, lambda
Storage :
4° C
Description :
CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test, Functional assay: Assay-Dependent, Neutralization: Assay-Dependent
Aliases :
AI528646; CD16; CD16A; CD16B; CD32; CD32 receptor 2; CD32B; CDw32; F630109E10Rik; Fc fragment of IgG receptor IIb; Fc fragment of IgG, low affinity IIb, receptor (CD32); fc gamma receptor IIB; Fc gamma receptor III; Fc gamma receptor RII; Fc gamma RIIB; Fc gamma RIIIa; Fc receptor, IgG, low affinity IIb; Fc receptor, IgG, low affinity III; Fc[g]RII; Fcg receptor III; FCG2; fc-gamma RII; Fc-gamma RII-b; Fc-gamma RII-c; Fc-gamma RIII; Fc-gamma-RII; FcgammaRIIB; fc-gamma-RIIB; Fc-gamma-RIIc; FcgammaRIII; Fcgr2; Fcgr2a; Fcgr2b; FCGR2C; FCGR3; FCGR3A; FCGR3B; FcgRII; FCGRIII; FCR-10; Fcr-2; Fcr-3; fcRII; FcRII-b; FcRII-c; FcRIII; FCRIIIA; FGFR2B; igG Fc receptor II beta; IgG Fc receptor III; low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; low affinity immunoglobulin gamma Fc region receptor III; Ly-17; Lym-1; Ly-m20; Lymphocyte antigen 17; RP11-5K23.1
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA