CD11a (LFA-1alpha) Monoclonal Antibody (M17/4), Functional Grade, eBioscience™ | Invitrogen 16-0111-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD11a (LFA-1alpha) Monoclonal Antibody (M17/4), Functional Grade, eBioscience™

catalog :

16-0111-82

quantity :

100 µg

price :

US 190

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

M17/4

reactivity :

mouse

application :

immunocytochemistry, flow cytometry, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 37

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:100; loading ...; fig 2f, 3g	Benkhoucha M, Tran N, Breville G, Senoner I, Bradfield P, Papayannopoulou T, et al. CD4+c-Met+Itgα4+ T cell subset promotes murine neuroinflammation. J Neuroinflammation. 2022;19:103 pubmed publisher
flow cytometry; mouse; loading ...	Hamminger P, Marchetti L, Preglej T, Platzer R, Zhu C, Kamnev A, et al. Histone deacetylase 1 controls CD4+ T cell trafficking in autoinflammatory diseases. J Autoimmun. 2021;119:102610 pubmed publisher
flow cytometry; mouse; loading ...; fig 2b	Muscate F, Stetter N, Schramm C, Schulze zur Wiesch J, Bosurgi L, Jacobs T. HVEM and CD160: Regulators of Immunopathology During Malaria Blood-Stage. Front Immunol. 2018;9:2611 pubmed publisher
flow cytometry; mouse; loading ...; fig 1c	Chu J, Mccormick B, Mazelyte G, Michael M, Vermeren S. HoxB8 neutrophils replicate Fcγ receptor and integrin-induced neutrophil signaling and functions. J Leukoc Biol. 2019;105:93-100 pubmed publisher
flow cytometry; mouse; 1:200; loading ...; fig 3a	Hailemichael Y, Woods A, Fu T, He Q, Nielsen M, Hasan F, et al. Cancer vaccine formulation dictates synergy with CTLA-4 and PD-L1 checkpoint blockade therapy. J Clin Invest. 2018;128:1338-1354 pubmed publisher
flow cytometry; mouse; loading ...; fig 3d	Capece T, Walling B, Lim K, Kim K, Bae S, Chung H, et al. A novel intracellular pool of LFA-1 is critical for asymmetric CD8+ T cell activation and differentiation. J Cell Biol. 2017;216:3817-3829 pubmed publisher
blocking or activating experiments; mouse; fig 8	Brinkman C, Iwami D, Hritzo M, Xiong Y, Ahmad S, Simon T, et al. Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration. Nat Commun. 2016;7:12021 pubmed publisher
flow cytometry; mouse; 1:150; fig s4	Ludigs K, Jandus C, Utzschneider D, Staehli F, Bessoles S, Dang A, et al. NLRC5 shields T lymphocytes from NK-cell-mediated elimination under inflammatory conditions. Nat Commun. 2016;7:10554 pubmed publisher
flow cytometry; mouse; fig 2	Moretto M, Khan I. IL-21 Is Important for Induction of KLRG1+ Effector CD8 T Cells during Acute Intracellular Infection. J Immunol. 2016;196:375-84 pubmed publisher
flow cytometry; mouse; fig 3	Zhang Z, Wu N, Lu Y, Davidson D, Colonna M, Veillette A. DNAM-1 controls NK cell activation via an ITT-like motif. J Exp Med. 2015;212:2165-82 pubmed publisher
flow cytometry; mouse; fig 2	Cabrera Perez J, Condotta S, James B, Kashem S, Brincks E, Rai D, et al. Alterations in antigen-specific naive CD4 T cell precursors after sepsis impairs their responsiveness to pathogen challenge. J Immunol. 2015;194:1609-20 pubmed publisher
flow cytometry; mouse; fig 2 immunocytochemistry; mouse; fig 2	Ueno N, Lodoen M, Hickey G, Robey E, Coombes J. Toxoplasma gondii-infected natural killer cells display a hypermotility phenotype in vivo. Immunol Cell Biol. 2015;93:508-13 pubmed publisher
flow cytometry; mouse; 1:100	Fu H, Kishore M, Gittens B, Wang G, Coe D, Komarowska I, et al. Self-recognition of the endothelium enables regulatory T-cell trafficking and defines the kinetics of immune regulation. Nat Commun. 2014;5:3436 pubmed publisher
flow cytometry; mouse	Misumi I, Whitmire J. B cell depletion curtails CD4+ T cell memory and reduces protection against disseminating virus infection. J Immunol. 2014;192:1597-608 pubmed publisher
flow cytometry; mouse	Hamdy S, Elamanchili P, Alshamsan A, Molavi O, Satou T, Samuel J. Enhanced antigen-specific primary CD4+ and CD8+ responses by codelivery of ovalbumin and toll-like receptor ligand monophosphoryl lipid A in poly(D,L-lactic-co-glycolic acid) nanoparticles. J Biomed Mater Res A. 2007;81:652-62 pubmed
	Pyka Fo x15b ciak G, Lis G, Litwin J. Adhesion Molecule Profile and the Effect of Anti-VLA-4 mAb Treatment in Experimental Autoimmune Encephalomyelitis, a Mouse Model of Multiple Sclerosis. Int J Mol Sci. 2022;23: pubmed publisher
	Tedford K, Steiner M, Koshutin S, Richter K, Tech L, Eggers Y, et al. The opposing forces of shear flow and sphingosine-1-phosphate control marginal zone B cell shuttling. Nat Commun. 2017;8:2261 pubmed publisher
	Ames R, Ting L, Gendlina I, Kim K, Macian F. The Transcription Factor NFAT1 Participates in the Induction of CD4+ T Cell Functional Exhaustion during Plasmodium yoelii Infection. Infect Immun. 2017;85: pubmed publisher
	Fontana M, Baccarella A, Craft J, Boyle M, McIntyre T, Wood M, et al. A Novel Model of Asymptomatic Plasmodium Parasitemia That Recapitulates Elements of the Human Immune Response to Chronic Infection. PLoS ONE. 2016;11:e0162132 pubmed publisher
	Guo H, Cranert S, Lu Y, Zhong M, Zhang S, Chen J, et al. Deletion of Slam locus in mice reveals inhibitory role of SLAM family in NK cell responses regulated by cytokines and LFA-1. J Exp Med. 2016;213:2187-207 pubmed publisher
	Hersrud S, Kovács A, Pearce D. Antigen presenting cell abnormalities in the Cln3(-/-) mouse model of juvenile neuronal ceroid lipofuscinosis. Biochim Biophys Acta. 2016;1862:1324-36 pubmed publisher
	Garcon F, Okkenhaug K. PI3K? promotes CD4(+) T-cell interactions with antigen-presenting cells by increasing LFA-1 binding to ICAM-1. Immunol Cell Biol. 2016;94:486-95 pubmed publisher
	Durward Diioia M, Harms J, Khan M, Hall C, Smith J, Splitter G. CD8+ T cell exhaustion, suppressed gamma interferon production, and delayed memory response induced by chronic Brucella melitensis infection. Infect Immun. 2015;83:4759-71 pubmed publisher
	Shindo Y, Unsinger J, Burnham C, Green J, Hotchkiss R. Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression. Shock. 2015;43:334-43 pubmed publisher
	Kim T, Kim M, Kim H, Lim S, Kim E, Kim K, et al. Homotypic NK cell-to-cell communication controls cytokine responsiveness of innate immune NK cells. Sci Rep. 2014;4:7157 pubmed publisher
	Harding M, Zhang K, Conly J, Kubes P. Neutrophil crawling in capillaries; a novel immune response to Staphylococcus aureus. PLoS Pathog. 2014;10:e1004379 pubmed publisher
	Verhagen J, Wraith D. Blockade of LFA-1 augments in vitro differentiation of antigen-induced Foxp3? Treg cells. J Immunol Methods. 2014;414:58-64 pubmed publisher
	Bodhankar S, Chen Y, Vandenbark A, Murphy S, Offner H. Treatment of experimental stroke with IL-10-producing B-cells reduces infarct size and peripheral and CNS inflammation in wild-type B-cell-sufficient mice. Metab Brain Dis. 2014;29:59-73 pubmed publisher
	Zumwalde N, Domae E, Mescher M, Shimizu Y. ICAM-1-dependent homotypic aggregates regulate CD8 T cell effector function and differentiation during T cell activation. J Immunol. 2013;191:3681-93 pubmed publisher
	Bankoti R, Gupta K, Levchenko A, Stager S. Marginal zone B cells regulate antigen-specific T cell responses during infection. J Immunol. 2012;188:3961-71 pubmed publisher
	Weiss K, Christiaansen A, Fulton R, Meyerholz D, Varga S. Multiple CD4+ T cell subsets produce immunomodulatory IL-10 during respiratory syncytial virus infection. J Immunol. 2011;187:3145-54 pubmed publisher
	Schmidt N, Butler N, Badovinac V, Harty J. Extreme CD8 T cell requirements for anti-malarial liver-stage immunity following immunization with radiation attenuated sporozoites. PLoS Pathog. 2010;6:e1000998 pubmed publisher
	Wernimont S, Simonson W, Greer P, Seroogy C, Huttenlocher A. Calpain 4 is not necessary for LFA-1-mediated function in CD4+ T cells. PLoS ONE. 2010;5:e10513 pubmed publisher
	Lin J, Huang J, Hung L, Wu S, Wu Hsieh B. Distinct roles of complement receptor 3, Dectin-1, and sialic acids in murine macrophage interaction with Histoplasma yeast. J Leukoc Biol. 2010;88:95-106 pubmed publisher
	Sarris M, Andersen K, Randow F, Mayr L, Betz A. Neuropilin-1 expression on regulatory T cells enhances their interactions with dendritic cells during antigen recognition. Immunity. 2008;28:402-13 pubmed publisher
	Kemball C, Harkins S, Whitton J. Enumeration and functional evaluation of virus-specific CD4+ and CD8+ T cells in lymphoid and peripheral sites of coxsackievirus B3 infection. J Virol. 2008;82:4331-42 pubmed publisher
	Back J, Chalifour A, Scarpellino L, Held W. Stable masking by H-2Dd cis ligand limits Ly49A relocalization to the site of NK cell/target cell contact. Proc Natl Acad Sci U S A. 2007;104:3978-83 pubmed

product information

Product Type :

Antibody

Product Name :

CD11a (LFA-1alpha) Monoclonal Antibody (M17/4), Functional Grade, eBioscience™

Catalog # :

16-0111-82

Quantity :

100 µg

Price :

US 190

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rat

Reactivity :

Mouse

Applications :

Control: Assay-Dependent, Flow Cytometry: 0.5 µg/test, Functional assay: Assay-Dependent

Species :

Mouse

Clone :

M17/4

Isotype :

IgG2a, kappa

Storage :

4° C

Description :

CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. CD11a is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of CD11a to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. CD11a is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of CD11a function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti- CD11a antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. Two transcript variants encoding different isoforms of CD11a have been found. Diseases associated with CD11a dysfunction include benign cephalic histiocytosis and leukocyte adhesion deficiency.

Format :

Liquid

Applications w/Dilutions :

Control: Assay-Dependent, Flow Cytometry: 0.5 µg/test, Functional assay: Assay-Dependent

Aliases :

(p180); alpha L integrin; antigen CD11A (p180); antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; antigen CD11A p180; CD11 antigen-like family member A; CD11a; integrin alpha L; integrin alphaL; integrin alpha-L; integrin alpha-L precursor; integrin gene promoter; integrin subunit alpha L; integrin surface receptor; integrin, alpha L; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); Itgal; Leukocyte adhesion glycoprotein LFA-1 alpha chain; Leukocyte function-associated molecule 1 alpha chain; LFA-1; LFA-1 alpha; LFA1A; LFA-1A; Ly-15; Ly-21; lymphocyte antigen 15; lymphocyte function associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen 1; lymphocyte function-associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen-1

more info or order :

Invitrogen product webpage