product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD4 Monoclonal Antibody (RPA-T4), Functional Grade, eBioscience
catalog :
16-0049-85
quantity :
500 ug
price :
US 399.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
RPA-T4
reactivity :
human, mouse
application :
immunohistochemistry, immunocytochemistry, neutralization, flow cytometry
more info or order :
citations: 257
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; fig 5b
Laurent E, Sieber A, Salzer B, Wachernig A, Seigner J, Lehner M, et al. Directed Evolution of Stabilized Monomeric CD19 for Monovalent CAR Interaction Studies and Monitoring of CAR-T Cell Patients. ACS Synth Biol. 2021;10:1184-1198 pubmed publisher
  • flow cytometry; human; loading ...; fig 2
Webb L, Fra Bido S, Innocentin S, Matheson L, Attaf N, Bignon A, et al. Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ. Aging Cell. 2021;20:e13295 pubmed publisher
  • flow cytometry; human; 1:100; loading ...
Deng M, Gui X, Kim J, Xie L, Chen W, Li Z, et al. LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration. Nature. 2018;562:605-609 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Hu X, Majchrzak K, Liu X, Wyatt M, Spooner C, Moisan J, et al. In Vitro Priming of Adoptively Transferred T Cells with a RORγ Agonist Confers Durable Memory and Stemness In Vivo. Cancer Res. 2018;78:3888-3898 pubmed publisher
  • flow cytometry; human; loading ...; fig 3b
Szabo P, Goswami A, Mazzuca D, Kim K, O Gorman D, Hess D, et al. Rapid and Rigorous IL-17A Production by a Distinct Subpopulation of Effector Memory T Lymphocytes Constitutes a Novel Mechanism of Toxic Shock Syndrome Immunopathology. J Immunol. 2017;198:2805-2818 pubmed publisher
  • flow cytometry; human; loading ...; fig 1
Zhen A, Rezek V, Youn C, Lam B, Chang N, Rick J, et al. Targeting type I interferon-mediated activation restores immune function in chronic HIV infection. J Clin Invest. 2017;127:260-268 pubmed publisher
  • flow cytometry; human; fig s3
Pachnio A, Ciáurriz M, Begum J, Lal N, Zuo J, Beggs A, et al. Cytomegalovirus Infection Leads to Development of High Frequencies of Cytotoxic Virus-Specific CD4+ T Cells Targeted to Vascular Endothelium. PLoS Pathog. 2016;12:e1005832 pubmed publisher
  • flow cytometry; human; fig s1
Harper I, Ali J, Harper S, Wlodek E, Alsughayyir J, Negus M, et al. Augmentation of Recipient Adaptive Alloimmunity by Donor Passenger Lymphocytes within the Transplant. Cell Rep. 2016;15:1214-27 pubmed publisher
  • immunohistochemistry; human; fig 1
Egan C, Sodhi C, Good M, Lin J, Jia H, Yamaguchi Y, et al. Toll-like receptor 4-mediated lymphocyte influx induces neonatal necrotizing enterocolitis. J Clin Invest. 2016;126:495-508 pubmed
  • flow cytometry; mouse
von Moltke J, Ji M, Liang H, Locksley R. Tuft-cell-derived IL-25 regulates an intestinal ILC2-epithelial response circuit. Nature. 2016;529:221-5 pubmed publisher
  • flow cytometry; human; fig 1
Japp A, Kursunel M, Meier S, Mälzer J, Li X, Rahman N, et al. Dysfunction of PSA-specific CD8+ T cells in prostate cancer patients correlates with CD38 and Tim-3 expression. Cancer Immunol Immunother. 2015;64:1487-94 pubmed publisher
  • flow cytometry; human
Lee J, Jeong I, Joh J, Jung Y, Sim S, Choi B, et al. Differential expression of CD57 in antigen-reactive CD4+ T cells between active and latent tuberculosis infection. Clin Immunol. 2015;159:37-46 pubmed publisher
  • flow cytometry; human; fig 1
Severson J, Serracino H, Mateescu V, Raeburn C, McIntyre R, Sams S, et al. PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer. Cancer Immunol Res. 2015;3:620-30 pubmed publisher
  • flow cytometry; mouse; fig 2
Vettermann C, Timblin G, Lim V, Lai E, Schlissel M. The proximal J kappa germline-transcript promoter facilitates receptor editing through control of ordered recombination. PLoS ONE. 2015;10:e0113824 pubmed publisher
  • flow cytometry; human
Van Eyck L, Hershfield M, Pombal D, Kelly S, Ganson N, Moens L, et al. Hematopoietic stem cell transplantation rescues the immunologic phenotype and prevents vasculopathy in patients with adenosine deaminase 2 deficiency. J Allergy Clin Immunol. 2015;135:283-7.e5 pubmed publisher
  • flow cytometry; human
Chow I, Yang J, Gates T, James E, Mai D, Greenbaum C, et al. Assessment of CD4+ T cell responses to glutamic acid decarboxylase 65 using DQ8 tetramers reveals a pathogenic role of GAD65 121-140 and GAD65 250-266 in T1D development. PLoS ONE. 2014;9:e112882 pubmed publisher
  • flow cytometry; human; tbl 1
Gerna G, Lilleri D, Fornara C, Bruno F, Gabanti E, Cane I, et al. Differential kinetics of human cytomegalovirus load and antibody responses in primary infection of the immunocompetent and immunocompromised host. J Gen Virol. 2015;96:360-9 pubmed publisher
  • flow cytometry; human
Abramowski P, Ogrodowczyk C, Martin R, Pongs O. A truncation variant of the cation channel P2RX5 is upregulated during T cell activation. PLoS ONE. 2014;9:e104692 pubmed publisher
  • immunocytochemistry; human; fig 2
Steiner S, Daniel C, Fischer A, Atreya I, Hirschmann S, Waldner M, et al. Cyclosporine A regulates pro-inflammatory cytokine production in ulcerative colitis. Arch Immunol Ther Exp (Warsz). 2015;63:53-63 pubmed publisher
  • flow cytometry; human
Kobie J, Treanor J, Ritchlin C. Transient decrease in human peripheral blood myeloid dendritic cells following influenza vaccination correlates with induction of serum antibody. Immunol Invest. 2014;43:606-15 pubmed publisher
  • flow cytometry; human
Pastille E, Bardini K, Fleissner D, Adamczyk A, Frede A, Wadwa M, et al. Transient ablation of regulatory T cells improves antitumor immunity in colitis-associated colon cancer. Cancer Res. 2014;74:4258-69 pubmed publisher
  • flow cytometry; human
Abramowski P, Otto B, Martin R. The orally available, synthetic ether lipid edelfosine inhibits T cell proliferation and induces a type I interferon response. PLoS ONE. 2014;9:e91970 pubmed publisher
  • flow cytometry; mouse
Timblin G, Schlissel M. Ebf1 and c-Myb repress rag transcription downstream of Stat5 during early B cell development. J Immunol. 2013;191:4676-87 pubmed publisher
  • flow cytometry; mouse; fig s1
Nussbaum J, Van Dyken S, von Moltke J, Cheng L, Mohapatra A, Molofsky A, et al. Type 2 innate lymphoid cells control eosinophil homeostasis. Nature. 2013;502:245-8 pubmed publisher
  • flow cytometry; human
Sharma S, Pichichero M. Functional deficits of pertussis-specific CD4+ T cells in infants compared to adults following DTaP vaccination. Clin Exp Immunol. 2012;169:281-91 pubmed publisher
  • flow cytometry; human
Liang H, Reinhardt R, Bando J, Sullivan B, Ho I, Locksley R. Divergent expression patterns of IL-4 and IL-13 define unique functions in allergic immunity. Nat Immunol. 2011;13:58-66 pubmed publisher
  • flow cytometry; human
Wang J, Kobie J, Zhang L, Cochran M, Mosmann T, Ritchlin C, et al. An 11-color flow cytometric assay for identifying, phenotyping, and assessing endocytic ability of peripheral blood dendritic cell subsets in a single platform. J Immunol Methods. 2009;341:106-16 pubmed publisher
Wan Y, Mu X, Zhao J, Li L, Xu W, Zhang M. Myeloid‑derived suppressor cell accumulation induces Treg expansion and modulates lung malignancy progression. Biomed Rep. 2024;20:68 pubmed publisher
Dai Q, Shi R, Zhang G, Wang Y, Ye L, Peng L, et al. miR-539-5p targets BMP2 to regulate Treg activation in B-cell acute lymphoblastic leukemia through TGF-β/Smads/MAPK. Exp Biol Med (Maywood). 2024;249:10111 pubmed publisher
Long J, Chen X, He M, Ou S, Zhao Y, Yan Q, et al. HLA-class II restricted TCR targeting human papillomavirus type 18 E7 induces solid tumor remission in mice. Nat Commun. 2024;15:2271 pubmed publisher
Xu C, Song Y, Liu W, Liu R, Bai Q, Li L, et al. IL-4 activates ULK1/Atg9a/Rab9 in asthma, NLRP3 inflammasomes, and Golgi fragmentation by increasing autophagy flux and mitochondrial oxidative stress. Redox Biol. 2024;71:103090 pubmed publisher
Col xf3 n D, Wanderley C, Turato W, Borges V, Franchin M, Castanheira F, et al. Paediatric sepsis survivors are resistant to sepsis-induced long-term immune dysfunction. Br J Pharmacol. 2024;181:1308-1323 pubmed publisher
Laquintana V, Mottini C, Marchesi F, Marcozzi B, Terrenato I, Sperandio E, et al. Dynamics of humoral and cellular response to three doses of anti-SARS-CoV-2 BNT162b2 vaccine in patients with hematological malignancies and older subjects. Front Immunol. 2023;14:1221587 pubmed publisher
Peng H, Lv Y, Li C, Cheng Z, He S, Wang C, et al. Cathepsin S inhibition in dendritic cells prevents Th17 cell differentiation in perivascular adipose tissues following vascular injury in diabetic rats. J Biochem Mol Toxicol. 2023;37:e23419 pubmed publisher
Zhang M, Chong K, Chen Z, Guo H, Liu Y, Kang Y, et al. Rapamycin improves Graves' orbitopathy by suppressing CD4+ cytotoxic T lymphocytes. JCI Insight. 2023;8: pubmed publisher
Zhong J, Ding R, Jiang H, Li L, Wan J, Feng X, et al. Single-cell RNA sequencing reveals the molecular features of peripheral blood immune cells in children, adults and centenarians. Front Immunol. 2022;13:1081889 pubmed publisher
McEvoy C, Murphy J, Zhang L, Clotet Freixas S, Mathews J, An J, et al. Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity. Nat Commun. 2022;13:7634 pubmed publisher
Rode A, Buus T, Mraz V, Al Jaberi F, Lopez D, Ford S, et al. Induced Human Regulatory T Cells Express the Glucagon-like Peptide-1 Receptor. Cells. 2022;11: pubmed publisher
Wang B, Wang C, Wan Y, Gao J, Ma Y, Zhang Y, et al. Decoding the pathogenesis of Diamond-Blackfan anemia using single-cell RNA-seq. Cell Discov. 2022;8:41 pubmed publisher
Kierkels G, van Diest E, Hern xe1 ndez L xf3 pez P, Scheper W, de Bruin A, Frijlink E, et al. Characterization and modulation of anti-αβTCR antibodies and their respective binding sites at the βTCR chain to enrich engineered T cells. Mol Ther Methods Clin Dev. 2021;22:388-400 pubmed publisher
Kim G, Kim W, Lim S, Lee H, Koo J, Nam K, et al. In Vivo Induction of Regulatory T Cells Via CTLA-4 Signaling Peptide to Control Autoimmune Encephalomyelitis and Prevent Disease Relapse. Adv Sci (Weinh). 2021;8:2004973 pubmed publisher
YEKU O, Rao T, Laster I, Kononenko A, Purdon T, Wang P, et al. Bispecific T-Cell Engaging Antibodies Against MUC16 Demonstrate Efficacy Against Ovarian Cancer in Monotherapy and in Combination With PD-1 and VEGF Inhibition. Front Immunol. 2021;12:663379 pubmed publisher
Prat M, Salon M, Allain T, Dubreuil O, Noël G, Preisser L, et al. Murlentamab, a Low Fucosylated Anti-Müllerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation. Cancers (Basel). 2021;13: pubmed publisher
Yoon H, Sung J, Song M. Effects of the Antidiabetic Drugs Evogliptin and Sitagliptin on the Immune Function of CD26/DPP4 in Th1 Cells. Biomol Ther (Seoul). 2021;29:154-165 pubmed publisher
Møller S, Mellergaard M, Madsen M, Bermejo A, Jepsen S, Hansen M, et al. Cytoplasmic Citrate Flux Modulates the Immune Stimulatory NKG2D Ligand MICA in Cancer Cells. Front Immunol. 2020;11:1968 pubmed publisher
He J, Xu Y, Yi M, Gu C, Zhu Y, Hu G. Involvement of natural killer cells in the pathogenesis of endometriosis in patients with pelvic pain. J Int Med Res. 2020;48:300060519871407 pubmed publisher
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Kim Y, Park S, Ha H, Lee A, Won H, Cha H, et al. HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment. J Cancer. 2020;11:4059-4072 pubmed publisher
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Thomas R, Shaath H, Naik A, Toor S, Elkord E, Decock J. Identification of two HLA-A*0201 immunogenic epitopes of lactate dehydrogenase C (LDHC): potential novel targets for cancer immunotherapy. Cancer Immunol Immunother. 2020;69:449-463 pubmed publisher
Randzavola L, Strege K, Juzans M, Asano Y, Stinchcombe J, Gawden Bone C, et al. Loss of ARPC1B impairs cytotoxic T lymphocyte maintenance and cytolytic activity. J Clin Invest. 2019;129:5600-5614 pubmed publisher
Lv M, Li Z, Liu J, Lin F, Zhang Q, Li Z, et al. MicroRNA‑155 inhibits the proliferation of CD8+ T cells via upregulating regulatory T cells in vitiligo. Mol Med Rep. 2019;20:3617-3624 pubmed publisher
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Petrelli A, Mijnheer G, Hoytema van Konijnenburg D, van der Wal M, Giovannone B, Mocholí E, et al. PD-1+CD8+ T cells are clonally expanding effectors in human chronic inflammation. J Clin Invest. 2018;128:4669-4681 pubmed publisher
Kononchik J, Ireland J, Zou Z, Segura J, Holzapfel G, Chastain A, et al. HIV-1 targets L-selectin for adhesion and induces its shedding for viral release. Nat Commun. 2018;9:2825 pubmed publisher
Jiang W, Zhong W, Deng Y, Chen C, Wang Q, Zhou M, et al. Evaluation of a combination "lymphocyte apoptosis model" to predict survival of sepsis patients in an intensive care unit. BMC Anesthesiol. 2018;18:89 pubmed publisher
Silva Neta H, Brelaz de Castro M, Chagas M, Mariz H, de Arruda R, de Vasconcelos V, et al. CD4+CD45RA-FOXP3low Regulatory T Cells as Potential Biomarkers of Disease Activity in Systemic Lupus Erythematosus Brazilian Patients. Biomed Res Int. 2018;2018:3419565 pubmed publisher
Porichis F, Hart M, Massa A, Everett H, Morou A, Richard J, et al. Immune Checkpoint Blockade Restores HIV-Specific CD4 T Cell Help for NK Cells. J Immunol. 2018;201:971-981 pubmed publisher
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Tedesco D, Thapa M, Chin C, Ge Y, Gong M, Li J, et al. Alterations in Intestinal Microbiota Lead to Production of Interleukin 17 by Intrahepatic γδ T-Cell Receptor-Positive Cells and Pathogenesis of Cholestatic Liver Disease. Gastroenterology. 2018;154:2178-2193 pubmed publisher
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Hegazy A, West N, Stubbington M, Wendt E, Suijker K, Datsi A, et al. Circulating and Tissue-Resident CD4+ T Cells With Reactivity to Intestinal Microbiota Are Abundant in Healthy Individuals and Function Is Altered During Inflammation. Gastroenterology. 2017;153:1320-1337.e16 pubmed publisher
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Nouri Shirazi M, Bible B, Zeng M, Tamjidi S, Bossart G. Phenotyping and comparing the immune cell populations of free-ranging Atlantic bottlenose dolphins (Tursiops truncatus) and dolphins under human care. BMC Vet Res. 2017;13:78 pubmed publisher
Su S, Liao J, Liu J, Huang D, He C, Chen F, et al. Blocking the recruitment of naive CD4+ T cells reverses immunosuppression in breast cancer. Cell Res. 2017;27:461-482 pubmed publisher
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Chen J, Wang Q, Feng X, Zhang Z, Geng L, Xu T, et al. Umbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria. Stem Cells Int. 2016;2016:4062789 pubmed publisher
Ribeiro S, Tesio M, Ribot J, Macintyre E, Barata J, Silva Santos B. Casein kinase 2 controls the survival of normal thymic and leukemic ?? T cells via promotion of AKT signaling. Leukemia. 2017;31:1603-1610 pubmed publisher
de Bourayne M, Gallais Y, El Ali Z, Rousseau P, Damiens M, Cochet C, et al. Protein kinase CK2 controls T-cell polarization through dendritic cell activation in response to contact sensitizers. J Leukoc Biol. 2017;101:703-715 pubmed publisher
Wang Y, Ma C, Ling Y, Bousfiha A, Camcioglu Y, Jacquot S, et al. Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations. J Exp Med. 2016;213:2413-2435 pubmed
Sugita S, Iwasaki Y, Makabe K, Kimura T, Futagami T, Suegami S, et al. Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors. Stem Cell Reports. 2016;7:619-634 pubmed publisher
Hunt L, Hensor E, Nam J, Burska A, Parmar R, Emery P, et al. T cell subsets: an immunological biomarker to predict progression to clinical arthritis in ACPA-positive individuals. Ann Rheum Dis. 2016;75:1884-9 pubmed publisher
La Porta J, Matus Nicodemos R, Valentin Acevedo A, Covey L. The RNA-Binding Protein, Polypyrimidine Tract-Binding Protein 1 (PTBP1) Is a Key Regulator of CD4 T Cell Activation. PLoS ONE. 2016;11:e0158708 pubmed publisher
Noack M, Ndongo Thiam N, Miossec P. Interaction among activated lymphocytes and mesenchymal cells through podoplanin is critical for a high IL-17 secretion. Arthritis Res Ther. 2016;18:148 pubmed publisher
Noack M, Ndongo Thiam N, Miossec P. Role of podoplanin in the high interleukin-17A secretion resulting from interactions between activated lymphocytes and psoriatic skin-derived mesenchymal cells. Clin Exp Immunol. 2016;186:64-74 pubmed publisher
Tang Z, Hao Y, Zhang E, Xu C, Zhou Y, Zheng X, et al. CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade. Mol Med Rep. 2016;14:1107-16 pubmed publisher
Zhao J, Ma J, Zhang X, Li Q, Yang X. Equilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance. Exp Ther Med. 2016;11:2300-2304 pubmed
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product information
Product Type :
Antibody
Product Name :
CD4 Monoclonal Antibody (RPA-T4), Functional Grade, eBioscience
Catalog # :
16-0049-85
Quantity :
500 ug
Price :
US 399.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 1 ug/test, Functional Assay: Assay-Dependent, Neutralization: Assay-Dependent
Species :
Human
Clone :
RPA-T4
Isotype :
IgG1, kappa
Storage :
4 C
Description :
The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1 ug/test, Functional Assay: Assay-Dependent, Neutralization: Assay-Dependent
Aliases :
Activation B7-1 antigen; B7; B7.1; B7-1; BB1; B-lymphocyte activation antigen B7; CD28LG; CD28LG1; CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; Cd4 molecule; CD4 precursor; CD4 receptor; CD4, allele 1; cd4a; CD4mut; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule; cell surface glycoprotein CD4; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; fCD4; L3T4; LAB7; Leu-3; Ly-4; lymphocyte antigen CD4; lymphocyte antigen CD4 precursor; membrane protein; p55; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); T-lymphocyte activation antigen CD80; W3/25; W3/25 antigen
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA