product summary
Loading...
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD3e Monoclonal Antibody (eBio500A2 (500A2)), Functional Grade, eBioscience
catalog :
16-0033-81
quantity :
50 µg
price :
US 97.50
clonality :
monoclonal
host :
Syrian golden hamster
conjugate :
nonconjugated
clone name :
eBio500A2 (500A2)
reactivity :
mouse
application :
immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 58
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 2d
Stebegg M, Bignon A, Hill D, Silva Cayetano A, Krueger C, Vanderleyden I, et al. Rejuvenating conventional dendritic cells and T follicular helper cell formation after vaccination. elife. 2020;9: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig 1a
Melo Gonzalez F, Kammoun H, Evren E, Dutton E, Papadopoulou M, Bradford B, et al. Antigen-presenting ILC3 regulate T cell-dependent IgA responses to colonic mucosal bacteria. J Exp Med. 2019;216:728-742 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 4a
Nayar S, Campos J, Smith C, Iannizzotto V, Gardner D, Colafrancesco S, et al. Phosphatidylinositol 3-kinase delta pathway: a novel therapeutic target for Sjögren's syndrome. Ann Rheum Dis. 2019;78:249-260 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig s1b
Moriyama S, Brestoff J, Flamar A, Moeller J, Klose C, Rankin L, et al. β2-adrenergic receptor-mediated negative regulation of group 2 innate lymphoid cell responses. Science. 2018;359:1056-1061 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1g
Siracusa F, McGrath M, Maschmeyer P, Bardua M, Lehmann K, Heinz G, et al. Nonfollicular reactivation of bone marrow resident memory CD4 T cells in immune clusters of the bone marrow. Proc Natl Acad Sci U S A. 2018;115:1334-1339 pubmed publisher
  • flow cytometry; mouse; 1:200; tbl 1
Jackson Jones L, Duncan S, Magalhaes M, Campbell S, Maizels R, McSorley H, et al. Fat-associated lymphoid clusters control local IgM secretion during pleural infection and lung inflammation. Nat Commun. 2016;7:12651 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 3a
Yamashita K, Kawata K, Matsumiya H, Kamekura R, Jitsukawa S, Nagaya T, et al. Bob1 limits cellular frequency of T-follicular helper cells. Eur J Immunol. 2016;46:1361-70 pubmed publisher
  • flow cytometry; mouse
Kaplan J, Marshall M, C McSkimming C, Harmon D, Garmey J, Oldham S, et al. Adipocyte progenitor cells initiate monocyte chemoattractant protein-1-mediated macrophage accumulation in visceral adipose tissue. Mol Metab. 2015;4:779-94 pubmed publisher
  • flow cytometry; mouse; 5 ug/ml
Hu Y, Xiao H, Shi T, Oppenheim J, Chen X. Progranulin promotes tumour necrosis factor-induced proliferation of suppressive mouse CD4? Foxp3? regulatory T cells. Immunology. 2014;142:193-201 pubmed publisher
  • flow cytometry; mouse; fig 1
Rommel P, Bosque D, Gitlin A, Croft G, Heintz N, Casellas R, et al. Fate mapping for activation-induced cytidine deaminase (AID) marks non-lymphoid cells during mouse development. PLoS ONE. 2013;8:e69208 pubmed publisher
  • flow cytometry; mouse; tbl 1
Zavitz C, Bauer C, Gaschler G, Fraser K, Strieter R, Hogaboam C, et al. Dysregulated macrophage-inflammatory protein-2 expression drives illness in bacterial superinfection of influenza. J Immunol. 2010;184:2001-13 pubmed publisher
  • flow cytometry; mouse; fig 1
Zou Y, Chen T, Han M, Wang H, Yan W, Song G, et al. Increased killing of liver NK cells by Fas/Fas ligand and NKG2D/NKG2D ligand contributes to hepatocyte necrosis in virus-induced liver failure. J Immunol. 2010;184:466-75 pubmed publisher
  • flow cytometry; mouse
MANICONE A, Huizar I, McGuire J. Matrilysin (Matrix Metalloproteinase-7) regulates anti-inflammatory and antifibrotic pulmonary dendritic cells that express CD103 (alpha(E)beta(7)-integrin). Am J Pathol. 2009;175:2319-31 pubmed publisher
Teng F, Tach xf3 Pi xf1 ot R, Sung B, Farber D, Worgall S, Hammad H, et al. ILC3s control airway inflammation by limiting T cell responses to allergens and microbes. Cell Rep. 2021;37:110051 pubmed publisher
Zhang X, Feng J, Chen S, Yang H, Dong Z. Synergized regulation of NK cell education by NKG2A and specific Ly49 family members. Nat Commun. 2019;10:5010 pubmed publisher
Compeer E, Kraus F, Ecker M, Redpath G, Amiezer M, Rother N, et al. A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation. Nat Commun. 2018;9:1597 pubmed publisher
Ibiza S, García Cassani B, Ribeiro H, Carvalho T, Almeida L, Marques R, et al. Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence. Nature. 2016;535:440-443 pubmed publisher
Casiraghi C, Márquez A, Shanina I, Horwitz M. Latent virus infection upregulates CD40 expression facilitating enhanced autoimmunity in a model of multiple sclerosis. Sci Rep. 2015;5:13995 pubmed publisher
Brown E, Wlodarska M, Willing B, Vonaesch P, Han J, Reynolds L, et al. Diet and specific microbial exposure trigger features of environmental enteropathy in a novel murine model. Nat Commun. 2015;6:7806 pubmed publisher
Song K, Herzog B, Fu J, Sheng M, Bergstrom K, McDaniel J, et al. Loss of Core 1-derived O-Glycans Decreases Breast Cancer Development in Mice. J Biol Chem. 2015;290:20159-66 pubmed publisher
Vijayachari P, Vedhagiri K, Mallilankaraman K, Mathur P, Sardesai N, Weiner D, et al. Immunogenicity of a novel enhanced consensus DNA vaccine encoding the leptospiral protein LipL45. Hum Vaccin Immunother. 2015;11:1945-53 pubmed publisher
Torabi Parizi P, Vrisekoop N, Kastenmuller W, Gerner M, Egen J, Germain R. Pathogen-related differences in the abundance of presented antigen are reflected in CD4+ T cell dynamic behavior and effector function in the lung. J Immunol. 2014;192:1651-1660 pubmed publisher
Chen X, Yang Y, Zhou Q, Weiss J, Howard O, McPherson J, et al. Effective chemoimmunotherapy with anti-TGF? antibody and cyclophosphamide in a mouse model of breast cancer. PLoS ONE. 2014;9:e85398 pubmed publisher
Eva M, Yuki K, Dauphinee S, Schwartzentruber J, Pyzik M, Paquet M, et al. Altered IFN-?-mediated immunity and transcriptional expression patterns in N-Ethyl-N-nitrosourea-induced STAT4 mutants confer susceptibility to acute typhoid-like disease. J Immunol. 2014;192:259-70 pubmed publisher
Zhou Q, Hu Y, Howard O, Oppenheim J, Chen X. In vitro generated Th17 cells support the expansion and phenotypic stability of CD4(+)Foxp3(+) regulatory T cells in vivo. Cytokine. 2014;65:56-64 pubmed publisher
Baird J, Fox B, Sanders K, Lizotte P, Cubillos Ruiz J, Scarlett U, et al. Avirulent Toxoplasma gondii generates therapeutic antitumor immunity by reversing immunosuppression in the ovarian cancer microenvironment. Cancer Res. 2013;73:3842-51 pubmed publisher
Mielke L, Jones S, Raverdeau M, Higgs R, Stefanska A, Groom J, et al. Retinoic acid expression associates with enhanced IL-22 production by ?? T cells and innate lymphoid cells and attenuation of intestinal inflammation. J Exp Med. 2013;210:1117-24 pubmed publisher
Huong L, Kobayashi M, Nakata M, Shioi G, Miyachi H, Honjo T, et al. In vivo analysis of Aicda gene regulation: a critical balance between upstream enhancers and intronic silencers governs appropriate expression. PLoS ONE. 2013;8:e61433 pubmed publisher
Eissler N, Mysliwietz J, Deppisch N, Ruf P, Lindhofer H, Mocikat R. Potential of the trifunctional bispecific antibody surek depends on dendritic cells: rationale for a new approach of tumor immunotherapy. Mol Med. 2013;19:54-61 pubmed publisher
Sepulveda F, Debeurme F, Menasche G, Kurowska M, Côte M, Pachlopnik Schmid J, et al. Distinct severity of HLH in both human and murine mutants with complete loss of cytotoxic effector PRF1, RAB27A, and STX11. Blood. 2013;121:595-603 pubmed publisher
Kulinski J, Leonardo S, Mounce B, Malherbe L, Gauld S, Tarakanova V. Ataxia telangiectasia mutated kinase controls chronic gammaherpesvirus infection. J Virol. 2012;86:12826-37 pubmed publisher
Parker D, Prince A. Staphylococcus aureus induces type I IFN signaling in dendritic cells via TLR9. J Immunol. 2012;189:4040-6 pubmed publisher
Parsa R, Andresen P, Gillett A, Mia S, Zhang X, Mayans S, et al. Adoptive transfer of immunomodulatory M2 macrophages prevents type 1 diabetes in NOD mice. Diabetes. 2012;61:2881-92 pubmed publisher
Haymaker C, Guloglu F, Cascio J, Hardaway J, Dhakal M, Wan X, et al. Bone marrow-derived IL-13R?1-positive thymic progenitors are restricted to the myeloid lineage. J Immunol. 2012;188:3208-16 pubmed publisher
Obata Y, Takahashi D, Ebisawa M, Kakiguchi K, Yonemura S, Jinnohara T, et al. Epithelial cell-intrinsic Notch signaling plays an essential role in the maintenance of gut immune homeostasis. J Immunol. 2012;188:2427-36 pubmed publisher
Akcay A, Nguyen Q, He Z, Turkmen K, Won Lee D, Hernando A, et al. IL-33 exacerbates acute kidney injury. J Am Soc Nephrol. 2011;22:2057-67 pubmed publisher
Tawara I, Nieves E, Liu C, Evers R, Toubai T, Sun Y, et al. Host basophils are dispensable for induction of donor T helper 2 cell differentiation and severity of experimental graft-versus-host disease. Biol Blood Marrow Transplant. 2011;17:1747-53 pubmed publisher
Kautz Neu K, Kostka S, Dinges S, Iwakura Y, Udey M, von Stebut E. A role for leukocyte-derived IL-1RA in DC homeostasis revealed by increased susceptibility of IL-1RA-deficient mice to cutaneous leishmaniasis. J Invest Dermatol. 2011;131:1650-9 pubmed publisher
Kim S, Han S, Withers D, Gaspal F, Bae J, Baik S, et al. CD117? CD3? CD56? OX40Lhigh cells express IL-22 and display an LTi phenotype in human secondary lymphoid tissues. Eur J Immunol. 2011;41:1563-72 pubmed publisher
Gardam S, Turner V, Anderton H, Limaye S, Basten A, Koentgen F, et al. Deletion of cIAP1 and cIAP2 in murine B lymphocytes constitutively activates cell survival pathways and inactivates the germinal center response. Blood. 2011;117:4041-51 pubmed publisher
Lochner M, Ohnmacht C, Presley L, Bruhns P, Si Tahar M, Sawa S, et al. Microbiota-induced tertiary lymphoid tissues aggravate inflammatory disease in the absence of RORgamma t and LTi cells. J Exp Med. 2011;208:125-34 pubmed publisher
Zhang X, Kang Y, Xiao L, Li R, Ding G, Wang Z, et al. Effects and mechanisms of tacrolimus on development of murine Th17 cells. Transplant Proc. 2010;42:3779-83 pubmed publisher
Boucheron N, Sharif O, Schebesta A, Croxford A, Raberger J, Schmidt U, et al. The protein tyrosine kinase Tec regulates a CD44highCD62L- Th17 subset. J Immunol. 2010;185:5111-9 pubmed publisher
Sun X, Wu Y, Gao W, Enjyoji K, Csizmadia E, Muller C, et al. CD39/ENTPD1 expression by CD4+Foxp3+ regulatory T cells promotes hepatic metastatic tumor growth in mice. Gastroenterology. 2010;139:1030-40 pubmed publisher
Chen X, Hamano R, Subleski J, Hurwitz A, Howard O, Oppenheim J. Expression of costimulatory TNFR2 induces resistance of CD4+FoxP3- conventional T cells to suppression by CD4+FoxP3+ regulatory T cells. J Immunol. 2010;185:174-82 pubmed publisher
Neill D, Wong S, Bellosi A, Flynn R, Daly M, Langford T, et al. Nuocytes represent a new innate effector leukocyte that mediates type-2 immunity. Nature. 2010;464:1367-70 pubmed publisher
Lahl K, Mayer C, Bopp T, Huehn J, Loddenkemper C, Eberl G, et al. Nonfunctional regulatory T cells and defective control of Th2 cytokine production in natural scurfy mutant mice. J Immunol. 2009;183:5662-72 pubmed publisher
Steinmetz O, Turner J, Paust H, Lindner M, Peters A, Heiss K, et al. CXCR3 mediates renal Th1 and Th17 immune response in murine lupus nephritis. J Immunol. 2009;183:4693-704 pubmed publisher
Lienenklaus S, Cornitescu M, Zietara N, Łyszkiewicz M, Gekara N, Jabłónska J, et al. Novel reporter mouse reveals constitutive and inflammatory expression of IFN-beta in vivo. J Immunol. 2009;183:3229-36 pubmed publisher
Tambuyzer B, Bergwerf I, De Vocht N, Reekmans K, Daans J, Jorens P, et al. Allogeneic stromal cell implantation in brain tissue leads to robust microglial activation. Immunol Cell Biol. 2009;87:267-73 pubmed publisher
Lahl K, Loddenkemper C, Drouin C, Freyer J, Arnason J, Eberl G, et al. Selective depletion of Foxp3+ regulatory T cells induces a scurfy-like disease. J Exp Med. 2007;204:57-63 pubmed
Rubinstein M, Kovar M, Purton J, Cho J, Boyman O, Surh C, et al. Converting IL-15 to a superagonist by binding to soluble IL-15R{alpha}. Proc Natl Acad Sci U S A. 2006;103:9166-71 pubmed
Hirrlinger P, Scheller A, Braun C, Hirrlinger J, Kirchhoff F. Temporal control of gene recombination in astrocytes by transgenic expression of the tamoxifen-inducible DNA recombinase variant CreERT2. Glia. 2006;54:11-20 pubmed
Day Y, Huang L, Ye H, Li L, Linden J, Okusa M. Renal ischemia-reperfusion injury and adenosine 2A receptor-mediated tissue protection: the role of CD4+ T cells and IFN-gamma. J Immunol. 2006;176:3108-14 pubmed
de Souza A, Oriss T, O Malley K, Ray A, Kane L. T cell Ig and mucin 1 (TIM-1) is expressed on in vivo-activated T cells and provides a costimulatory signal for T cell activation. Proc Natl Acad Sci U S A. 2005;102:17113-8 pubmed
Ortaldo J, Winkler Pickett R, Mason A, Mason L. The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells. J Immunol. 1998;160:1158-65 pubmed
Havran W, Poenie M, Kimura J, Tsien R, Weiss A, Allison J. Expression and function of the CD3-antigen receptor on murine CD4+8+ thymocytes. Nature. 1987;330:170-3 pubmed
Portoles P, Rojo J, Golby A, Bonneville M, Gromkowski S, Greenbaum L, et al. Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation. J Immunol. 1989;142:4169-75 pubmed
product information
Product Type :
Antibody
Product Name :
CD3e Monoclonal Antibody (eBio500A2 (500A2)), Functional Grade, eBioscience
Catalog # :
16-0033-81
Quantity :
50 µg
Price :
US 97.50
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Syrian Hamster
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.5 µg/test, Functional Assay: Assay-Dependent
Species :
Mouse
Clone :
eBio500A2 (500A2)
Isotype :
IgG
Storage :
4° C
Description :
The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test, Functional Assay: Assay-Dependent
Aliases :
4930549J05Rik; A430104F18Rik; AI504783; antigen CD3D, delta polypeptide (TiT3 complex); antigen CD3E, epsilon polypeptide (TiT3 complex); antigen CD3G, gamma polypeptide; antigen CD3Z, zeta polypeptide; AW552088; Cd247; CD247 antigen; CD247 antigen, zeta subunit; Cd247 molecule; CD3; CD3 antigen delta chain; CD3 antigen delta polypeptide; CD3 antigen gamma chain; CD3 antigen, delta polypeptide; CD3 antigen, delta subunit; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 delta; CD3 epsilon; CD3 epsilon chain; CD3 epsilon subunit; CD3 epsilon subunit precursor; CD3 gamma-chain; CD3 glycoprotein; CD3 glycoprotein precursor; CD3 molecule delta polypeptide; CD3 molecule, delta; CD3 molecule, epsilon; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma; CD3 molecule, gamma polypeptide; CD3 protein; CD3 TCR complex; CD3 type I transmembrane glycoprotein; CD3 type I transmembrane glycoprotein precursor; CD3 zeta chain; Cd3d; CD3D antigen delta; CD3D antigen, delta polypeptide (TiT3 complex); CD3d molecule; CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; Cd3e; CD3E antigen, epsilon polypeptide; CD3E antigen, epsilon polypeptide (TiT3 complex); CD3e molecule; CD3e molecule, epsilon (CD3-TCR complex); CD3epsilon; CD3-epsilon; Cd3-eta; Cd3g; CD3G antigen, gamma polypeptide; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule; CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3-GAMMA; Cd3h; CD3Q; Cd3z; CD3Z antigen, zeta polypeptide (TiT3 complex); Cd3zeta; Cd3-zeta; CD3zeta chain; CD3-zeta/eta; Ctg3; Ctg-3; FLJ18683; IMD17; IMD18; IMD19; IMD25; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T3/TCR complex; T3d; T3e; T3g; T3Z; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor CD3 epsilon chain; T-cell receptor CD3 epsilon subunit; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell receptor zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T-cell surface protein; TcR CD3 delta-chain; TcR CD3 gamma-chain; TCR zeta chain; TCR zeta chain subunit; TCRE; Tcrk; TCRZ; TCRzeta; TiT3 complex; type I transmembrane protein; T-cell surface molecule CD3
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA