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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD25 Monoclonal Antibody (PC61.5), PE-Cyanine5, eBioscience™
catalog :
15-0251-82
quantity :
100 µg
price :
280 USD
clonality :
monoclonal
host :
rat
conjugate :
PE-Cy5
clone name :
PC61.5
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 54
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; fig 7B
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Xu W, Cherrier D, Chea S, Vosshenrich C, Serafini N, Petit M, et al. An Id2RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials. Immunity. 2019;50:1054-1068.e3 pubmed publisher
Xu Y, Tang X, Yang M, Zhang S, Li S, Chen Y, et al. Interleukin 10 Gene-Modified Bone Marrow-Derived Dendritic Cells Attenuate Liver Fibrosis in Mice by Inducing Regulatory T Cells and Inhibiting the TGF-β/Smad Signaling Pathway. Mediators Inflamm. 2019;2019:4652596 pubmed publisher
Li Q, Shan Q, Sang X, Zhu R, Chen X, Cao G. Total Glycosides of Peony Protects Against Inflammatory Bowel Disease by Regulating IL-23/IL-17 Axis and Th17/Treg Balance. Am J Chin Med. 2019;47:177-201 pubmed publisher
Ng K, Yui M, Mehta A, Siu S, Irwin B, Pease S, et al. A stochastic epigenetic switch controls the dynamics of T-cell lineage commitment. elife. 2018;7: pubmed publisher
Chen J, Yang N, Liu H, Yao H, Wang J, Yang Y, et al. Immunological effects of a low-dose cytarabine, aclarubicin and granulocyte-colony stimulating factor priming regimen on a mouse leukemia model. Oncol Lett. 2018;16:3022-3028 pubmed publisher
Manresa Arraut A, Johansen F, Brakebusch C, Issazadeh Navikas S, Hasseldam H. RhoA Drives T-Cell Activation and Encephalitogenic Potential in an Animal Model of Multiple Sclerosis. Front Immunol. 2018;9:1235 pubmed publisher
Kiyota T, Machhi J, Lu Y, Dyavarshetty B, Nemati M, Yokoyama I, et al. Granulocyte-macrophage colony-stimulating factor neuroprotective activities in Alzheimer's disease mice. J Neuroimmunol. 2018;319:80-92 pubmed publisher
Wei Y, Zhang Z, Wang F, Zhou S. Assessment of tumor growth factor-β1 neutralizing antibody in the treatment of allergic rhinitis and asthma. Exp Ther Med. 2018;15:649-656 pubmed publisher
Hammer A, Waschbisch A, Knippertz I, Zinser E, Berg J, Jörg S, et al. Role of Nuclear Factor (Erythroid-Derived 2)-Like 2 Signaling for Effects of Fumaric Acid Esters on Dendritic Cells. Front Immunol. 2017;8:1922 pubmed publisher
Tamura R, Uemoto S, Tabata Y. Immunosuppressive effect of mesenchymal stem cell-derived exosomes on a concanavalin A-induced liver injury model. Inflamm Regen. 2016;36:26 pubmed publisher
Wang Q, Ning H, Peng H, Wei L, Hou R, Hoft D, et al. Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses. Nat Commun. 2017;8:867 pubmed publisher
Alzubi J, Pallant C, Mussolino C, Howe S, Thrasher A, Cathomen T. Targeted genome editing restores T cell differentiation in a humanized X-SCID pluripotent stem cell disease model. Sci Rep. 2017;7:12475 pubmed publisher
Hammer A, Schliep A, Jörg S, Haghikia A, Gold R, Kleinewietfeld M, et al. Impact of combined sodium chloride and saturated long-chain fatty acid challenge on the differentiation of T helper cells in neuroinflammation. J Neuroinflammation. 2017;14:184 pubmed publisher
Levine A, Mendoza A, Hemmers S, Moltedo B, Niec R, Schizas M, et al. Stability and function of regulatory T cells expressing the transcription factor T-bet. Nature. 2017;546:421-425 pubmed publisher
Yang H, Guo H, Li X, Lin J, Zhang W, Zhao J, et al. Viral RNA-Unprimed Rig-I Restrains Stat3 Activation in the Modulation of Regulatory T Cell/Th17 Cell Balance. J Immunol. 2017;199:119-128 pubmed publisher
Chen J, Zhang M, Zhou F, Wang J, Niu B, Zhang W. Immunological effects of vaccines combined with granulocyte colony-stimulating factor on a murine WEHI-3 leukemia model. Oncol Lett. 2017;13:2323-2329 pubmed publisher
Zhu F, Willette Brown J, Song N, Lomada D, Song Y, Xue L, et al. Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis. Cell Host Microbe. 2017;21:478-493.e7 pubmed publisher
Scharschmidt T, Vasquez K, Pauli M, Leitner E, Chu K, Truong H, et al. Commensal Microbes and Hair Follicle Morphogenesis Coordinately Drive Treg Migration into Neonatal Skin. Cell Host Microbe. 2017;21:467-477.e5 pubmed publisher
Ruan G, Tao B, Wang D, Li Y, Wu J, Yin G. Chinese herbal medicine formula Gu-Ben-Fang-Xiao-Tang attenuates airway inflammation by modulating Th17/Treg balance in an ovalbumin-induced murine asthma model. Exp Ther Med. 2016;12:1428-1434 pubmed
Stifter K, Schuster C, Schlosser M, Boehm B, Schirmbeck R. Exploring the induction of preproinsulin-specific Foxp3(+) CD4(+) Treg cells that inhibit CD8(+) T cell-mediated autoimmune diabetes by DNA vaccination. Sci Rep. 2016;6:29419 pubmed publisher
Allison K, Sajti E, Collier J, Gosselin D, Troutman T, Stone E, et al. Affinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells. elife. 2016;5: pubmed publisher
Lee H, Kim J, Quan W, Lee J, Kim M, Kim S, et al. Autophagy deficiency in myeloid cells increases susceptibility to obesity-induced diabetes and experimental colitis. Autophagy. 2016;12:1390-403 pubmed publisher
Brinkman C, Iwami D, Hritzo M, Xiong Y, Ahmad S, Simon T, et al. Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration. Nat Commun. 2016;7:12021 pubmed publisher
Gu L, Deng W, Sun X, Zhou H, Xu Q. Rapamycin ameliorates CCl4-induced liver fibrosis in mice through reciprocal regulation of the Th17/Treg cell balance. Mol Med Rep. 2016;14:1153-61 pubmed publisher
Carofino B, Ayanga B, Tracey L, Brooke Bisschop T, Justice M. PRDM14 promotes RAG-dependent Notch1 driver mutations in mouse T-ALL. Biol Open. 2016;5:645-53 pubmed publisher
Yue Y, Li P, Song N, Li B, Li Z, Guo Y, et al. Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model. Mol Med Rep. 2016;13:4183-90 pubmed publisher
Traka M, Podojil J, McCarthy D, Miller S, Popko B. Oligodendrocyte death results in immune-mediated CNS demyelination. Nat Neurosci. 2016;19:65-74 pubmed publisher
Zhong Y, Tang H, Wang X, Zeng Q, Liu Y, Zhao X, et al. Intranasal immunization with heat shock protein 60 induces CD4(+) CD25(+) GARP(+) and type 1 regulatory T cells and inhibits early atherosclerosis. Clin Exp Immunol. 2016;183:452-68 pubmed publisher
Kawahara M, Takaku H. A tumor lysate is an effective vaccine antigen for the stimulation of CD4(+) T-cell function and subsequent induction of antitumor immunity mediated by CD8(+) T cells. Cancer Biol Ther. 2015;16:1616-25 pubmed publisher
Laidlaw B, Cui W, Amezquita R, Gray S, Guan T, Lu Y, et al. Production of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells. Nat Immunol. 2015;16:871-9 pubmed publisher
Tähtinen S, Kaikkonen S, Merisalo Soikkeli M, Grönberg Vähä Koskela S, Kanerva A, Parviainen S, et al. Favorable alteration of tumor microenvironment by immunomodulatory cytokines for efficient T-cell therapy in solid tumors. PLoS ONE. 2015;10:e0131242 pubmed publisher
Khan I, Perrard X, Brunner G, Lui H, Sparks L, Smith S, et al. Intermuscular and perimuscular fat expansion in obesity correlates with skeletal muscle T cell and macrophage infiltration and insulin resistance. Int J Obes (Lond). 2015;39:1607-18 pubmed publisher
Li Q, Zou J, Wang M, Ding X, Chepelev I, Zhou X, et al. Critical role of histone demethylase Jmjd3 in the regulation of CD4+ T-cell differentiation. Nat Commun. 2014;5:5780 pubmed publisher
Chan M, Chang C, Tung C, Sun J, Suen J, Wu S. Low-dose 5-aza-2'-deoxycytidine pretreatment inhibits experimental autoimmune encephalomyelitis by induction of regulatory T cells. Mol Med. 2014;20:248-56 pubmed publisher
Liu H, Liu Z, Chao H, Li Z, Song Z, Yang Y, et al. High-dose interferon-? promotes abortion in mice by suppressing Treg and Th17 polarization. J Interferon Cytokine Res. 2014;34:394-403 pubmed publisher
Zanello G, Goethel A, Forster K, Geddes K, Philpott D, Croitoru K. Nod2 activates NF-kB in CD4+ T cells but its expression is dispensable for T cell-induced colitis. PLoS ONE. 2013;8:e82623 pubmed publisher
Diaz de Durana Y, Lau J, Knee D, Filippi C, Londei M, McNamara P, et al. IL-2 immunotherapy reveals potential for innate beta cell regeneration in the non-obese diabetic mouse model of autoimmune diabetes. PLoS ONE. 2013;8:e78483 pubmed publisher
Martin B, Auffray C, Delpoux A, Pommier A, Durand A, Charvet C, et al. Highly self-reactive naive CD4 T cells are prone to differentiate into regulatory T cells. Nat Commun. 2013;4:2209 pubmed publisher
Roy S, Stevens M, So L, Edinger A. Reciprocal effects of rab7 deletion in activated and neglected T cells. Autophagy. 2013;9:1009-23 pubmed publisher
Guo C, Kogan S, Amano S, Wang M, Dagdeviren S, Friedline R, et al. CD40 deficiency in mice exacerbates obesity-induced adipose tissue inflammation, hepatic steatosis, and insulin resistance. Am J Physiol Endocrinol Metab. 2013;304:E951-63 pubmed publisher
Gupta S, Marcel N, Sarin A, Shivashankar G. Role of actin dependent nuclear deformation in regulating early gene expression. PLoS ONE. 2012;7:e53031 pubmed publisher
Wan W, Lionakis M, Liu Q, Roffe E, Murphy P. Genetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice. Cardiovasc Res. 2013;97:580-8 pubmed publisher
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Wang R, Xie H, Huang Z, Ma J, Fang X, Ding Y, et al. T cell factor 1 regulates thymocyte survival via a RORγt-dependent pathway. J Immunol. 2011;187:5964-73 pubmed publisher
Zhen Y, Sun L, Liu H, Duan K, Zeng C, Zhang L, et al. Alterations of peripheral CD4+CD25+Foxp3+ T regulatory cells in mice with STZ-induced diabetes. Cell Mol Immunol. 2012;9:75-85 pubmed publisher
Liu C, Ye P, Yen B, Miao C. In vivo expansion of regulatory T cells with IL-2/IL-2 mAb complexes prevents anti-factor VIII immune responses in hemophilia A mice treated with factor VIII plasmid-mediated gene therapy. Mol Ther. 2011;19:1511-20 pubmed publisher
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Chen G, Han G, Feng J, Wang J, Wang R, Xu R, et al. Glutamic acid decarboxylase-derived epitopes with specific domains expand CD4(+)CD25(+) regulatory T cells. PLoS ONE. 2009;4:e7034 pubmed publisher
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Hayashi T, Hasegawa K, Adachi C. Elimination of CD4(+)CD25(+) T cell accelerates the development of glomerulonephritis during the preactive phase in autoimmune-prone female NZB x NZW F mice. Int J Exp Pathol. 2005;86:289-96 pubmed
product information
Product Type :
Antibody
Product Name :
CD25 Monoclonal Antibody (PC61.5), PE-Cyanine5, eBioscience™
Catalog # :
15-0251-82
Quantity :
100 µg
Price :
280 USD
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.125 µg/test
Species :
Mouse
Clone :
PC61.5
Isotype :
IgG1, lambda
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.125 µg/test
Aliases :
CD25; cytokine receptor; IDDM10; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; il-2 receptor alpha; IL-2 Receptor alpha chain; IL-2 receptor alpha subunit; IL2 receptor subunit alpha; IL-2 receptor subunit alpha; IL2R; IL-2R alpha; IL-2R alpha chain; IL2R subunit alpha; IL-2R subunit alpha; Il2ra; IL-2RA; IL-2-RA; IL2-RA; Il2ra antibody; IL2RAC; IMD41; interleukin 2 receptor; interleukin 2 receptor alpha chain; interleukin 2 receptor subunit alpha; interleukin 2 receptor, alpha; interleukin 2 receptor, alpha chain; interleukin-2 receptor alpha; interleukin-2 receptor alpha chain; interleukin-2 receptor beta chain; Interleukin2 receptor subunit alpha; interleukin-2 receptor subunit alpha; Ly-43; p55; p55 chain; sIL 2R; soluble IL 2 receptor; TAC antigen; TCGFR
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA