product summary
Loading...
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Perforin Monoclonal Antibody (dG9 (delta G9)), eBioscience
catalog :
14-9994-82
quantity :
100 µg
price :
US 341
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
dG9 (delta G9)
reactivity :
human, pigs
application :
immunohistochemistry, immunoprecipitation, flow cytometry
more info or order :
Invitrogen product webpage
citations: 24
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 3a
Gorvel L, Korenfeld D, Tung T, Klechevsky E. Dendritic Cell-Derived IL-32?: A Novel Inhibitory Cytokine of NK Cell Function. J Immunol. 2017;199:1290-1300 pubmed publisher
  • flow cytometry; human; loading ...; fig 3d
Tong A, Hashem H, Eid S, Allen F, Kingsley D, Huang A. Adoptive natural killer cell therapy is effective in reducing pulmonary metastasis of Ewing sarcoma. Oncoimmunology. 2017;6:e1303586 pubmed publisher
  • flow cytometry; human; loading ...; fig 3b
Collins A, Rothman N, Liu K, Reiner S. Eomesodermin and T-bet mark developmentally distinct human natural killer cells. JCI Insight. 2017;2:e90063 pubmed publisher
  • flow cytometry; human; loading ...; fig 1e
Ryan P, Sumaria N, Holland C, Bradford C, Izotova N, Grandjean C, et al. Heterogeneous yet stable Vδ2(+) T-cell profiles define distinct cytotoxic effector potentials in healthy human individuals. Proc Natl Acad Sci U S A. 2016;113:14378-14383 pubmed
  • flow cytometry; human; loading ...; fig 4a
Srivastava R, Khan A, Garg S, Syed S, Furness J, Vahed H, et al. Human Asymptomatic Epitopes Identified from the Herpes Simplex Virus Tegument Protein VP13/14 (UL47) Preferentially Recall Polyfunctional Effector Memory CD44high CD62Llow CD8+ TEM Cells and Protect Humanized HLA-A*02:01 Transgenic Mice against Ocula. J Virol. 2017;91: pubmed publisher
  • flow cytometry; human; fig 3
Yin W, Tong S, Zhang Q, Shao J, Liu Q, Peng H, et al. Functional dichotomy of Vδ2 γδ T cells in chronic hepatitis C virus infections: role in cytotoxicity but not for IFN-γ production. Sci Rep. 2016;6:26296 pubmed publisher
  • flow cytometry; human; fig 3
Marquardt N, Béziat V, Nyström S, Hengst J, Ivarsson M, Kekäläinen E, et al. Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells. J Immunol. 2015;194:2467-71 pubmed publisher
  • flow cytometry; human
Jiang B, Wu X, Li X, Yang X, Zhou Y, Yan H, et al. Expansion of NK cells by engineered K562 cells co-expressing 4-1BBL and mMICA, combined with soluble IL-21. Cell Immunol. 2014;290:10-20 pubmed publisher
  • flow cytometry; human
Randall K, Chan S, Ma C, Fung I, Mei Y, Yabas M, et al. DOCK8 deficiency impairs CD8 T cell survival and function in humans and mice. J Exp Med. 2011;208:2305-20 pubmed publisher
Kim H, Kim H, Nam M, Park J, Lee M, Chung S, et al. Suppression of the antitumoral activity of natural killer cells under indirect coculture with cancer-associated fibroblasts in a pancreatic TIME-on-chip model. Cancer Cell Int. 2023;23:219 pubmed publisher
Galat Y, Du Y, Perepitchka M, Li X, Balyasnikova I, Tse W, et al. In vitro vascular differentiation system efficiently produces natural killer cells for cancer immunotherapies. Oncoimmunology. 2023;12:2240670 pubmed publisher
Lao L, Zeng W, Huang P, Chen H, Jia Z, Wang P, et al. CD8+ T cell-Dependent Remodeling of the Tumor Microenvironment Overcomes Chemoresistance. Cancer Immunol Res. 2023;11:320-338 pubmed publisher
Zhang M, Chong K, Chen Z, Guo H, Liu Y, Kang Y, et al. Rapamycin improves Graves' orbitopathy by suppressing CD4+ cytotoxic T lymphocytes. JCI Insight. 2023;8: pubmed publisher
Liu X, Jin S, Hu S, Li R, Pan H, Liu Y, et al. Single-cell transcriptomics links malignant T cells to the tumor immune landscape in cutaneous T cell lymphoma. Nat Commun. 2022;13:1158 pubmed publisher
Li H, Yang Z, Yang X, Zhang F, Wang J, Wu Z, et al. LINC01123 promotes immune escape by sponging miR-214-3p to regulate B7-H3 in head and neck squamous-cell carcinoma. Cell Death Dis. 2022;13:109 pubmed publisher
Singh N, Frey N, Engels B, Barrett D, Shestova O, Ravikumar P, et al. Antigen-independent activation enhances the efficacy of 4-1BB-costimulated CD22 CAR T cells. Nat Med. 2021;27:842-850 pubmed publisher
Rha M, Jeong H, Ko J, Choi S, Seo I, Lee J, et al. PD-1-Expressing SARS-CoV-2-Specific CD8+ T Cells Are Not Exhausted, but Functional in Patients with COVID-19. Immunity. 2021;54:44-52.e3 pubmed publisher
Billiet L, Goetgeluk G, Bonte S, De Munter S, De Cock L, Pille M, et al. Human Thymic CD10+ PD-1+ Intraepithelial Lymphocyte Precursors Acquire Interleukin-15 Responsiveness at the CD1a- CD95+ CD28- CCR7- Developmental Stage. Int J Mol Sci. 2020;21: pubmed publisher
Komlósi Z, Kovacs N, Van De Veen W, Kirsch A, Fahrner H, Wawrzyniak M, et al. Human CD40 ligand-expressing type 3 innate lymphoid cells induce IL-10-producing immature transitional regulatory B cells. J Allergy Clin Immunol. 2018;142:178-194.e11 pubmed publisher
Seebauer C, Brunner S, Glockzin G, Piso P, Ruemmele P, Schlitt H, et al. Peritoneal carcinomatosis of colorectal cancer is characterized by structural and functional reorganization of the tumor microenvironment inducing senescence and proliferation arrest in cancer cells. Oncoimmunology. 2016;5:e1242543 pubmed publisher
Su S, Liao J, Liu J, Huang D, He C, Chen F, et al. Blocking the recruitment of naive CD4+ T cells reverses immunosuppression in breast cancer. Cell Res. 2017;27:461-482 pubmed publisher
Komori M, Lin Y, Cortese I, Blake A, Ohayon J, Cherup J, et al. Insufficient disease inhibition by intrathecal rituximab in progressive multiple sclerosis. Ann Clin Transl Neurol. 2016;3:166-79 pubmed publisher
Barnig C, Cernadas M, Dutile S, Liu X, Perrella M, Kazani S, et al. Lipoxin A4 regulates natural killer cell and type 2 innate lymphoid cell activation in asthma. Sci Transl Med. 2013;5:174ra26 pubmed publisher
Hameed A, Olsen K, Cheng L, Fox W, Hruban R, Podack E. Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody. Am J Pathol. 1992;140:1025-30 pubmed
product information
Product Type :
Antibody
Product Name :
Perforin Monoclonal Antibody (dG9 (delta G9)), eBioscience
Catalog # :
14-9994-82
Quantity :
100 µg
Price :
US 341
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human, Porcine
Applications :
Immunohistochemistry: Assay-Dependent, Immunoprecipitation: Assay-Dependent
Species :
Human, Porcine
Clone :
dG9 (delta G9)
Isotype :
IgG2b, kappa
Storage :
4° C
Description :
Perforin is one of the major cytolytic proteins of cytolytic granules. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes. Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.
Format :
Liquid
Applications w/Dilutions :
Immunohistochemistry: Assay-Dependent, Immunoprecipitation: Assay-Dependent
Aliases :
Cyta; Cytolysin; FLH2; HPLH2; lymphocyte pore forming protein; lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; P1; perforin 1; perforin 1 (pore forming protein); Perforin1; perforin-1; Pfn; PFN1; Pfp; PGFL; PIGF; PIGF-2; PLGF; pore forming protein; pore-forming protein; PRF1; Prf-1; PRF1 (pore forming protein 1); RATCYTA; RP11-710A11.3
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA