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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD279 (PD-1) Monoclonal Antibody (J116), eBioscience
catalog :
14-9989-82
quantity :
100 µg
price :
US 247
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
J116
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
Invitrogen product webpage
citations: 41
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...
Deng M, Gui X, Kim J, Xie L, Chen W, Li Z, et al. LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration. Nature. 2018;562:605-609 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:25; loading ...; fig 1
Chen Z, Mei J, Liu L, Wang G, Li Z, Hou J, et al. PD-L1 expression is associated with advanced non-small cell lung cancer. Oncol Lett. 2016;12:921-927 pubmed
  • western blot; human; fig 2d
Yoon K, Byun S, Kwon E, Hwang S, Chu K, Hiraki M, et al. Control of signaling-mediated clearance of apoptotic cells by the tumor suppressor p53. Science. 2015;349:1261669 pubmed publisher
  • blocking or activating experiments; human; 10 ug/ml
Sawada Y, Yoshikawa T, Shimomura M, Iwama T, Endo I, Nakatsura T. Programmed death-1 blockade enhances the antitumor effects of peptide vaccine-induced peptide-specific cytotoxic T lymphocytes. Int J Oncol. 2015;46:28-36 pubmed publisher
  • flow cytometry; human; fig 1
Raptopoulou A, Bertsias G, Makrygiannakis D, Verginis P, Kritikos I, Tzardi M, et al. The programmed death 1/programmed death ligand 1 inhibitory pathway is up-regulated in rheumatoid synovium and regulates peripheral T cell responses in human and murine arthritis. Arthritis Rheum. 2010;62:1870-80 pubmed publisher
Dergham A, Vaz de Paula C, Nagashima S, Olandoski M, de Noronha L, Sotomaior V. Immunohistochemical Profiling of PD-1, PD-L1, CD8, MSI, and p53 and Prognostic Implications in Advanced Serous Ovarian Carcinoma: A Retrospective Study. J Pers Med. 2023;13: pubmed publisher
Islam M, Sevak J, Sharma M, Jindal A, Vyas A, Bajpai M, et al. Immune predictors of hepatitis B surface antigen seroconversion in patients with hepatitis B reactivation. Aliment Pharmacol Ther. 2023;57:689-708 pubmed publisher
Liang Q, Tong L, Xiang L, Shen S, Pan C, Liu C, et al. Correlations of the expression of γδ T cells and their co-stimulatory molecules TIGIT, PD-1, ICOS and BTLA with PR and PIBF in the peripheral blood and decidual tissues of women with unexplained recurrent spontaneous abortion. Clin Exp Immunol. 2021;203:55-65 pubmed publisher
Sabins N, Chornoguz O, Leander K, Kaplan F, Carter R, Kinder M, et al. TIM-3 Engagement Promotes Effector Memory T Cell Differentiation of Human Antigen-Specific CD8 T Cells by Activating mTORC1. J Immunol. 2017;199:4091-4102 pubmed publisher
Hock B, MacPherson S, McKenzie J. Idelalisib and caffeine reduce suppression of T cell responses mediated by activated chronic lymphocytic leukemia cells. PLoS ONE. 2017;12:e0172858 pubmed publisher
Tjin E, Krebbers G, Meijlink K, van de Kasteele W, Rosenberg E, Sanders J, et al. Immune-escape markers in relation to clinical outcome of advanced melanoma patients following immunotherapy. Cancer Immunol Res. 2014;2:538-46 pubmed publisher
Singh A, Mohan A, Dey A, Mitra D. Inhibiting the programmed death 1 pathway rescues Mycobacterium tuberculosis-specific interferon ?-producing T cells from apoptosis in patients with pulmonary tuberculosis. J Infect Dis. 2013;208:603-15 pubmed publisher
Sherman A, Trehanpati N, Daucher M, Davey R, Masur H, Sarin S, et al. Augmentation of hepatitis B virus-specific cellular immunity with programmed death receptor-1/programmed death receptor-L1 blockade in hepatitis B virus and HIV/hepatitis B virus coinfected patients treated with adefovir. AIDS Res Hum Retroviruses. 2013;29:665-72 pubmed publisher
Sakhno L, Tikhonova M, Tyrinova T, Leplina O, Shevela E, Nikonov S, et al. Cytotoxic activity of dendritic cells as a possible mechanism of negative regulation of T lymphocytes in pulmonary tuberculosis. Clin Dev Immunol. 2012;2012:628635 pubmed publisher
Singh A, Dey A, Mohan A, Sharma P, Mitra D. Foxp3+ regulatory T cells among tuberculosis patients: impact on prognosis and restoration of antigen specific IFN-? producing T cells. PLoS ONE. 2012;7:e44728 pubmed publisher
Jurado J, Pasquinelli V, Alvarez I, Peña D, Rovetta A, Tateosian N, et al. IL-17 and IFN-? expression in lymphocytes from patients with active tuberculosis correlates with the severity of the disease. J Leukoc Biol. 2012;91:991-1002 pubmed publisher
Zhang E, Zhang X, Liu J, Wang B, Tian Y, Kosinska A, et al. The expression of PD-1 ligands and their involvement in regulation of T cell functions in acute and chronic woodchuck hepatitis virus infection. PLoS ONE. 2011;6:e26196 pubmed publisher
Wang B, Bao J, Wang J, Wang Y, Jiang M, Xing M, et al. Immunostaining of PD-1/PD-Ls in liver tissues of patients with hepatitis and hepatocellular carcinoma. World J Gastroenterol. 2011;17:3322-9 pubmed publisher
Tjin E, Konijnenberg D, Krebbers G, Mallo H, Drijfhout J, Franken K, et al. T-cell immune function in tumor, skin, and peripheral blood of advanced stage melanoma patients: implications for immunotherapy. Clin Cancer Res. 2011;17:5736-47 pubmed publisher
Otani T, Tsuji Takayama K, Okochi A, Yamamoto M, Takeuchi M, Yamasaki F, et al. Stromal cells' B7-1 is a key stimulatory molecule for interleukin-10 production by HOZOT, a multifunctional regulatory T-cell line. Immunol Cell Biol. 2011;89:246-54 pubmed publisher
Alvarez I, Pasquinelli V, Jurado J, Abbate E, Musella R, de la Barrera S, et al. Role played by the programmed death-1-programmed death ligand pathway during innate immunity against Mycobacterium tuberculosis. J Infect Dis. 2010;202:524-32 pubmed publisher
Cacere C, Mendes Giannini M, Fontes C, Kono A, Duarte A, Benard G. Altered expression of the costimulatory molecules CD80, CD86, CD152, PD-1 and ICOS on T-cells from paracoccidioidomycosis patients: lack of correlation with T-cell hyporesponsiveness. Clin Immunol. 2008;129:341-9 pubmed publisher
Jurado J, Alvarez I, Pasquinelli V, Martinez G, Quiroga M, Abbate E, et al. Programmed death (PD)-1:PD-ligand 1/PD-ligand 2 pathway inhibits T cell effector functions during human tuberculosis. J Immunol. 2008;181:116-25 pubmed
Taglauer E, Trikhacheva A, Slusser J, Petroff M. Expression and function of PDCD1 at the human maternal-fetal interface. Biol Reprod. 2008;79:562-9 pubmed publisher
Ghebeh H, Barhoush E, Tulbah A, Elkum N, Al Tweigeri T, Dermime S. FOXP3+ Tregs and B7-H1+/PD-1+ T lymphocytes co-infiltrate the tumor tissues of high-risk breast cancer patients: Implication for immunotherapy. BMC Cancer. 2008;8:57 pubmed publisher
Wiesemann E, Deb M, Trebst C, Hemmer B, Stangel M, Windhagen A. Effects of interferon-beta on co-signaling molecules: upregulation of CD40, CD86 and PD-L2 on monocytes in relation to clinical response to interferon-beta treatment in patients with multiple sclerosis. Mult Scler. 2008;14:166-76 pubmed
Geng L, Jiang G, Fang Y, Dong S, Xie H, Chen Y, et al. B7-H1 expression is upregulated in peripheral blood CD14+ monocytes of patients with chronic hepatitis B virus infection, which correlates with higher serum IL-10 levels. J Viral Hepat. 2006;13:725-33 pubmed
LaGier A, Pober J. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum Immunol. 2006;67:568-78 pubmed
Salih H, Wintterle S, Krusch M, Kroner A, Huang Y, Chen L, et al. The role of leukemia-derived B7-H1 (PD-L1) in tumor-T-cell interactions in humans. Exp Hematol. 2006;34:888-94 pubmed
Chen Y, Zhang J, Li J, Zou L, Zhao T, Tang Y, et al. Expression of B7-H1 in inflammatory renal tubular epithelial cells. Nephron Exp Nephrol. 2006;102:e81-92 pubmed
Kroner A, Mehling M, Hemmer B, Rieckmann P, Toyka K, Maurer M, et al. A PD-1 polymorphism is associated with disease progression in multiple sclerosis. Ann Neurol. 2005;58:50-7 pubmed
Ding H, Wu X, Gao W. PD-L1 is expressed by human renal tubular epithelial cells and suppresses T cell cytokine synthesis. Clin Immunol. 2005;115:184-91 pubmed
Saudemont A, Jouy N, Hetuin D, Quesnel B. NK cells that are activated by CXCL10 can kill dormant tumor cells that resist CTL-mediated lysis and can express B7-H1 that stimulates T cells. Blood. 2005;105:2428-35 pubmed
Godfrey W, Spoden D, Ge Y, Baker S, Liu B, Levine B, et al. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Blood. 2005;105:750-8 pubmed
Hatachi S, Iwai Y, Kawano S, Morinobu S, Kobayashi M, Koshiba M, et al. CD4+ PD-1+ T cells accumulate as unique anergic cells in rheumatoid arthritis synovial fluid. J Rheumatol. 2003;30:1410-9 pubmed
Iwai Y, Okazaki T, Nishimura H, Kawasaki A, Yagita H, Honjo T. Microanatomical localization of PD-1 in human tonsils. Immunol Lett. 2002;83:215-20 pubmed
Dong H, Strome S, Salomao D, Tamura H, Hirano F, Flies D, et al. Tumor-associated B7-H1 promotes T-cell apoptosis: a potential mechanism of immune evasion. Nat Med. 2002;8:793-800 pubmed
Latchman Y, Wood C, Chernova T, Chaudhary D, Borde M, Chernova I, et al. PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2001;2:261-8 pubmed
Freeman G, Long A, Iwai Y, Bourque K, Chernova T, Nishimura H, et al. Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med. 2000;192:1027-34 pubmed
Nishimura H, Agata Y, Kawasaki A, Sato M, Imamura S, Minato N, et al. Developmentally regulated expression of the PD-1 protein on the surface of double-negative (CD4-CD8-) thymocytes. Int Immunol. 1996;8:773-80 pubmed
Agata Y, Kawasaki A, Nishimura H, Ishida Y, Tsubata T, Yagita H, et al. Expression of the PD-1 antigen on the surface of stimulated mouse T and B lymphocytes. Int Immunol. 1996;8:765-72 pubmed
product information
Product Type :
Antibody
Product Name :
CD279 (PD-1) Monoclonal Antibody (J116), eBioscience
Catalog # :
14-9989-82
Quantity :
100 µg
Price :
US 247
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 1 µg/test, Functional Assay: Assay-Dependent, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent
Species :
Human
Clone :
J116
Isotype :
IgG1, kappa
Storage :
4° C
Description :
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1 µg/test, Functional Assay: Assay-Dependent, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent
Aliases :
CD279; EGK_05005; hPD1; hPD-1; hPD-l; hSLE1; Ly101; mPD-1; PD1; PD-1; Pdc1; Pdcd1; programmed cell death 1; programmed cell death 1 protein; programmed cell death protein 1; programmed cell death protein 1-like; programmed death 1; Protein PD1; protein PD-1; sCD279; SLEB2; soluble CD279; systemic lupus erythematosus susceptibility 2
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA