HLA-E Monoclonal Antibody (3D12HLA-E), eBioscience | Invitrogen 14-9953-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HLA-E Monoclonal Antibody (3D12HLA-E), eBioscience

catalog :

14-9953-82

quantity :

100 µg

price :

US 248.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3D12HLA-E

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human	Lim D, Yawata N, Selva K, Li N, Tsai C, Yeong L, et al. The combination of type I IFN, TNF-Î±, and cell surface receptor engagement with dendritic cells enables NK cells to overcome immune evasion by dengue virus. J Immunol. 2014;193:5065-75 pubmed publisher
	Le Roy A, Prebet T, Castellano R, Goubard A, Riccardi F, Fauriat C, et al. Immunomodulatory Drugs Exert Anti-Leukemia Effects in Acute Myeloid Leukemia by Direct and Immunostimulatory Activities. Front Immunol. 2018;9:977 pubmed publisher
	Gornalusse G, Hirata R, Funk S, Riolobos L, Lopes V, Manske G, et al. HLA-E-expressing pluripotent stem cells escape allogeneic responses and lysis by NK cells. Nat Biotechnol. 2017;35:765-772 pubmed publisher
	Veluchamy J, Heeren A, Spanholtz J, van Eendenburg J, Heideman D, Kenter G, et al. High-efficiency lysis of cervical cancer by allogeneic NK cells derived from umbilical cord progenitors is independent of HLA status. Cancer Immunol Immunother. 2017;66:51-61 pubmed publisher
	Griffin B, Gram A, Mulder A, van Leeuwen D, Claas F, Wang F, et al. EBV BILF1 evolved to downregulate cell surface display of a wide range of HLA class I molecules through their cytoplasmic tail. J Immunol. 2013;190:1672-84 pubmed publisher
	Tjwa E, van Oord G, Biesta P, Boonstra A, Janssen H, Woltman A. Restoration of TLR3-activated myeloid dendritic cell activity leads to improved natural killer cell function in chronic hepatitis B virus infection. J Virol. 2012;86:4102-9 pubmed publisher
	Enqvist M, Nilsonne G, Hammarfjord O, Wallin R, Björkström N, Björnstedt M, et al. Selenite induces posttranscriptional blockade of HLA-E expression and sensitizes tumor cells to CD94/NKG2A-positive NK cells. J Immunol. 2011;187:3546-54 pubmed publisher
	Ravindranath M, Pham T, El Awar N, Kaneku H, Terasaki P. Anti-HLA-E mAb 3D12 mimics MEM-E/02 in binding to HLA-B and HLA-C alleles: Web-tools validate the immunogenic epitopes of HLA-E recognized by the antibodies. Mol Immunol. 2011;48:423-30 pubmed publisher
	Wooden S, Kalb S, Cotter R, Soloski M. Cutting edge: HLA-E binds a peptide derived from the ATP-binding cassette transporter multidrug resistance-associated protein 7 and inhibits NK cell-mediated lysis. J Immunol. 2005;175:1383-7 pubmed
	Strong R, Holmes M, Li P, Braun L, Lee N, Geraghty D. HLA-E allelic variants. Correlating differential expression, peptide affinities, crystal structures, and thermal stabilities. J Biol Chem. 2003;278:5082-90 pubmed
	Lee N, Llano M, Carretero M, Ishitani A, Navarro F, Lopez Botet M, et al. HLA-E is a major ligand for the natural killer inhibitory receptor CD94/NKG2A. Proc Natl Acad Sci U S A. 1998;95:5199-204 pubmed

product information

Product Type :

Antibody

Product Name :

HLA-E Monoclonal Antibody (3D12HLA-E), eBioscience

Catalog # :

14-9953-82

Quantity :

100 µg

Price :

US 248.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 1 µg/test, Immunoprecipitation: Assay-Dependent, Western Blot: Assay-Dependent

Species :

Human

Clone :

3D12HLA-E

Isotype :

IgG1, kappa

Storage :

4° C

Description :

HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1 µg/test, Immunoprecipitation: Assay-Dependent, Western Blot: Assay-Dependent

Aliases :

A29 protein; antigen presenting molecule; Aw-33; Aw-74; DAMA-277I14.1; DAQB-90C11.16; DKFZp686P19218; EA1.2; EA2.1; FLJ26655; HLA A; HLA class I antigen; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-30 alpha chain; HLA class I histocompatibility antigen, A-33 alpha chain; HLA class I histocompatibility antigen, A-74 alpha chain; HLA class I histocompatibility antigen, alpha chain E; HLA class I histocompatibility antigen, E alpha chain; HLA locus; HLA-6.2; HLAA; HLA-A; HLA-A locus alpha 2 domain; HLA-A null; HLA-A11; HLA-A33; HLA-Aw33.1; HLA-DQB1; HLA-DRB1; HLAE; HLA-E; Human leukocyte antigen A; leukocyte antigen class I-A; lymphocyte antigen; major histocompatibility complex, class I, A; major histocompatibility complex, class I, E; MCH class I antigen; MHC; MHC 1; MHC class 1 antigen; MHC class I anti; MHC Class I antigen; MHC class I antigen A*30; MHC class I antigen A*33; MHC class I antigen A*74; MHC class I antigen E; MHC class I antigen heavy chain; MHC class I antigen HLA-A heavy chain; MHC class I antigen HLA-A33; MHC class I antigen null protein; MHC class I antigene; MHC class II antigen; MHC classI antigen; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC1; QA1; sHLA-E; Soluble HLA class I histocompatibility antigen, alpha chain E; truncated MHC class I antigen

more info or order :

Invitrogen product webpage