Caspase 8 Monoclonal Antibody (1H10), eBioscience™ | Invitrogen 14-9934-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Caspase 8 Monoclonal Antibody (1H10), eBioscience™

catalog :

14-9934-82

quantity :

100 µg

price :

US 350

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

1H10

reactivity :

human

application :

western blot

more info or order :

Invitrogen product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000	Meng P, Yoshida H, Tanji K, Matsumiya T, Xing F, Hayakari R, et al. Carnosic acid attenuates apoptosis induced by amyloid-Î² 1-42 or 1-43 in SH-SY5Y human neuroblastoma cells. Neurosci Res. 2015;94:1-9 pubmed publisher
	Sanchez I, Xu C, Juo P, Kakizaka A, Blenis J, Yuan J. Caspase-8 is required for cell death induced by expanded polyglutamine repeats. Neuron. 1999;22:623-33 pubmed
	Li H, Zhu H, Xu C, Yuan J. Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis. Cell. 1998;94:491-501 pubmed

product information

Product Type :

Antibody

Product Name :

Caspase 8 Monoclonal Antibody (1H10), eBioscience™

Catalog # :

14-9934-82

Quantity :

100 µg

Price :

US 350

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rat

Reactivity :

Human

Applications :

Western Blot: 1-5 µg/mL

Species :

Human

Clone :

1H10

Isotype :

IgG2a

Storage :

4° C

Description :

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases play a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a pro-domain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. Caspase 8 is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of Caspase 8 suggests that it may interact with Fas-interacting protein FADD. Caspase 8 was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the pro-form of the Caspase 8 protein. Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby committing the cell to undergo apoptosis. In addition, Caspase 8 also reacts with Jurkat cells and Tonsil. Overexpression of Caspase 8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family. Many alternatively spliced transcript variants encoding different isoforms have been described for Caspase 8, however, not all variants have had their full-length sequences determined.

Format :

Liquid

Applications w/Dilutions :

Western Blot: 1-5 µg/mL

Aliases :

ALPS2B; apoptosis-related cysteine peptidase; apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; Casp8; Casp-8; caspase 8; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; Caspase8; caspase-8; Caspase-8 precursor; Caspase-8 subunit p10; Caspase-8 subunit p18; EC 3.4.22.61; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; Fas-linked ICE-like protease; FLICE; FLJ17672; ICE8; ICE-like apoptotic protease 5; MACH; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; Mch5; MGC78473; MORT1-associated ced-3 homolog; OTTHUMP00000163720; OTTHUMP00000165063; OTTHUMP00000206557; OTTHUMP00000206581

more info or order :

Invitrogen product webpage