CD282 (TLR2) Monoclonal Antibody (TL2.1), eBioscience™ | Invitrogen 14-9922-80 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD282 (TLR2) Monoclonal Antibody (TL2.1), eBioscience™

catalog :

14-9922-80

quantity :

25 µg

price :

US 138

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

TL2.1

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 63

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; fig 6	SnÃ¤ll J, LinnÃ©r A, Uhlmann J, Siemens N, Ibold H, Janos M, et al. Differential neutrophil responses to bacterial stimuli: Streptococcal strains are potent inducers of heparin-binding protein and resistin-release. Sci Rep. 2016;6:21288 pubmed publisher
blocking or activating experiments; human; 1 ug/ml; fig 3	Nagy L, Grishina I, Macal M, Hirao L, Hu W, Sankaran Walters S, et al. Chronic HIV infection enhances the responsiveness of antigen presenting cells to commensal Lactobacillus. PLoS ONE. 2013;8:e72789 pubmed publisher
flow cytometry; human; fig 8	Laudanski K, De A, Miller Graziano C. Exogenous heat shock protein 27 uniquely blocks differentiation of monocytes to dendritic cells. Eur J Immunol. 2007;37:2812-24 pubmed
flow cytometry; human	Sendide K, Reiner N, Lee J, Bourgoin S, Talal A, Hmama Z. Cross-talk between CD14 and complement receptor 3 promotes phagocytosis of mycobacteria: regulation by phosphatidylinositol 3-kinase and cytohesin-1. J Immunol. 2005;174:4210-9 pubmed
	Hsu S, Chang M, Lin S, Ko Y, Chou L, Tian Y, et al. Peptidoglycan mediates Leptospira outer membrane protein Loa22 to toll-like receptor 2 for inflammatory interaction: a novel innate immune recognition. Sci Rep. 2021;11:1064 pubmed publisher
	Aguilar Briseño J, Upasani V, Ellen B, Moser J, Pauzuolis M, Ruiz Silva M, et al. TLR2 on blood monocytes senses dengue virus infection and its expression correlates with disease pathogenesis. Nat Commun. 2020;11:3177 pubmed publisher
	Bahraoui E, Serrero M, Planès R. HIV-1 Tat - TLR4/MD2 interaction drives the expression of IDO-1 in monocytes derived dendritic cells through NF-κB dependent pathway. Sci Rep. 2020;10:8177 pubmed publisher
	Chen Y, Huang Z, Chen X, Ye H. Activation of the Toll‑like receptor 2 signaling pathway inhibits the proliferation of HCC cells in vitro. Oncol Rep. 2019;42:2267-2278 pubmed publisher
	Faraj T, Stover C, Erridge C. Dietary Toll-Like Receptor Stimulants Promote Hepatic Inflammation and Impair Reverse Cholesterol Transport in Mice via Macrophage-Dependent Interleukin-1 Production. Front Immunol. 2019;10:1404 pubmed publisher
	Wang Y, Chen C, Qi J, Wu F, Guan J, Chen Z, et al. Altered PGE2-EP2 is associated with an excessive immune response in HBV-related acute-on-chronic liver failure. J Transl Med. 2019;17:93 pubmed publisher
	Wang F, Li Y, Yang C, Mu Y, Wang Y, Zhang W, et al. Mannan-Binding Lectin Suppresses Peptidoglycan-Induced TLR2 Activation and Inflammatory Responses. Mediators Inflamm. 2019;2019:1349784 pubmed publisher
	Graustein A, Misch E, Musvosvi M, Shey M, Shah J, Seshadri C, et al. Toll-like receptor chaperone HSP90B1 and the immune response to Mycobacteria. PLoS ONE. 2018;13:e0208940 pubmed publisher
	Nazli A, Dizzell S, Zahoor M, Ferreira V, Kafka J, Woods M, et al. Interferon-β induced in female genital epithelium by HIV-1 glycoprotein 120 via Toll-like-receptor 2 pathway acts to protect the mucosal barrier. Cell Mol Immunol. 2019;16:178-194 pubmed publisher
	Wu B, Wang L, Jiang L, Dong L, Xu F, Lu Y, et al. n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils. Mol Med Rep. 2017;: pubmed publisher
	Hielpos M, Ferrero M, FernÃ¡ndez A, Bonetto J, Giambartolomei G, Fossati C, et al. CCL20 and Beta-Defensin 2 Production by Human Lung Epithelial Cells and Macrophages in Response to Brucella abortus Infection. PLoS ONE. 2015;10:e0140408 pubmed publisher
	Wang M, Wang F, Yang J, Zhao D, Wang H, Shao F, et al. Mannan-binding lectin inhibits Candida albicans-induced cellular responses in PMA-activated THP-1 cells through Toll-like receptor 2 and Toll-like receptor 4. PLoS ONE. 2013;8:e83517 pubmed publisher
	Wei Y, Xia W, Ye X, Fan Y, Shi J, Wen W, et al. The antimicrobial protein short palate, lung, and nasal epithelium clone 1 (SPLUNC1) is differentially modulated in eosinophilic and noneosinophilic chronic rhinosinusitis with nasal polyps. J Allergy Clin Immunol. 2014;133:420-8 pubmed publisher
	Ben Haij N, Leghmari K, Planès R, Thieblemont N, Bahraoui E. HIV-1 Tat protein binds to TLR4-MD2 and signals to induce TNF-? and IL-10. Retrovirology. 2013;10:123 pubmed publisher
	Nazli A, Kafka J, Ferreira V, Anipindi V, Mueller K, Osborne B, et al. HIV-1 gp120 induces TLR2- and TLR4-mediated innate immune activation in human female genital epithelium. J Immunol. 2013;191:4246-58 pubmed publisher
	Sanchez A, Espinosa P, Garcia T, Mancilla R. The 19 kDa Mycobacterium tuberculosis lipoprotein (LpqH) induces macrophage apoptosis through extrinsic and intrinsic pathways: a role for the mitochondrial apoptosis-inducing factor. Clin Dev Immunol. 2012;2012:950503 pubmed publisher
	Al ofi E, Coffelt S, Anumba D. Monocyte subpopulations from pre-eclamptic patients are abnormally skewed and exhibit exaggerated responses to Toll-like receptor ligands. PLoS ONE. 2012;7:e42217 pubmed publisher
	Olliver M, Hiew J, Mellroth P, Henriques Normark B, Bergman P. Human monocytes promote Th1 and Th17 responses to Streptococcus pneumoniae. Infect Immun. 2011;79:4210-7 pubmed publisher
	Scian R, Barrionuevo P, Giambartolomei G, De Simone E, Vanzulli S, Fossati C, et al. Potential role of fibroblast-like synoviocytes in joint damage induced by Brucella abortus infection through production and induction of matrix metalloproteinases. Infect Immun. 2011;79:3619-32 pubmed publisher
	Liang J, Jiang D, Jung Y, Xie T, Ingram J, Church T, et al. Role of hyaluronan and hyaluronan-binding proteins in human asthma. J Allergy Clin Immunol. 2011;128:403-411.e3 pubmed publisher
	Coenen M, Nischalke H, Kramer B, Langhans B, Glässner A, Schulte D, et al. Hepatitis C virus core protein induces fibrogenic actions of hepatic stellate cells via toll-like receptor 2. Lab Invest. 2011;91:1375-82 pubmed publisher
	Borde C, Barnay Verdier S, Gaillard C, Hocini H, Marechal V, Gozlan J. Stepwise release of biologically active HMGB1 during HSV-2 infection. PLoS ONE. 2011;6:e16145 pubmed publisher
	Zughaier S. Neisseria meningitidis capsular polysaccharides induce inflammatory responses via TLR2 and TLR4-MD-2. J Leukoc Biol. 2011;89:469-80 pubmed publisher
	Perez Barcena J, Crespí C, Regueiro V, Marsé P, Raurich J, Ibáñez J, et al. Lack of effect of glutamine administration to boost the innate immune system response in trauma patients in the intensive care unit. Crit Care. 2010;14:R233 pubmed publisher
	Quevedo Diaz M, Song C, Xiong Y, Chen H, Wahl L, Radulovic S, et al. Involvement of TLR2 and TLR4 in cell responses to Rickettsia akari. J Leukoc Biol. 2010;88:675-85 pubmed publisher
	Ding J, Rapista A, Teleshova N, Mosoyan G, Jarvis G, Klotman M, et al. Neisseria gonorrhoeae enhances HIV-1 infection of primary resting CD4+ T cells through TLR2 activation. J Immunol. 2010;184:2814-24 pubmed publisher
	Monaco C, Gregan S, Navin T, Foxwell B, Davies A, Feldmann M. Toll-like receptor-2 mediates inflammation and matrix degradation in human atherosclerosis. Circulation. 2009;120:2462-9 pubmed publisher
	Wenink M, Santegoets K, Broen J, van Bon L, Abdollahi Roodsaz S, Popa C, et al. TLR2 promotes Th2/Th17 responses via TLR4 and TLR7/8 by abrogating the type I IFN amplification loop. J Immunol. 2009;183:6960-70 pubmed publisher
	Takahashi K, Sugi Y, Hosono A, Kaminogawa S. Epigenetic regulation of TLR4 gene expression in intestinal epithelial cells for the maintenance of intestinal homeostasis. J Immunol. 2009;183:6522-9 pubmed publisher
	Tukel C, Wilson R, Nishimori J, Pezeshki M, Chromy B, BAUMLER A. Responses to amyloids of microbial and host origin are mediated through toll-like receptor 2. Cell Host Microbe. 2009;6:45-53 pubmed publisher
	Shim H, Kim J, Kim M, Sim H, Park D, Sohn J, et al. Legionella lipoprotein activates toll-like receptor 2 and induces cytokine production and expression of costimulatory molecules in peritoneal macrophages. Exp Mol Med. 2009;41:687-94 pubmed publisher
	Hajishengallis G, Wang M, Liang S, Triantafilou M, Triantafilou K. Pathogen induction of CXCR4/TLR2 cross-talk impairs host defense function. Proc Natl Acad Sci U S A. 2008;105:13532-7 pubmed publisher
	Lee H, Takeshita T, Shimada J, Akopyan A, Woo J, Pan H, et al. Induction of beta defensin 2 by NTHi requires TLR2 mediated MyD88 and IRAK-TRAF6-p38MAPK signaling pathway in human middle ear epithelial cells. BMC Infect Dis. 2008;8:87 pubmed publisher
	Burgener I, Jungi T. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008;124:184-91 pubmed publisher
	Do J, Kwon S, Park S, Lee E. Effects of vitamin D on expression of Toll-like receptors of monocytes from patients with Behcet's disease. Rheumatology (Oxford). 2008;47:840-8 pubmed publisher
	Mandron M, Ariès M, Boralevi F, Martin H, Charveron M, Taieb A, et al. Age-related differences in sensitivity of peripheral blood monocytes to lipopolysaccharide and Staphylococcus aureus toxin B in atopic dermatitis. J Invest Dermatol. 2008;128:882-9 pubmed
	Bonnefont Rebeix C, Marchal T, Bernaud J, Pin J, Leroux C, Lebecque S, et al. Toll-like receptor 3 (TLR3): a new marker of canine monocytes-derived dendritic cells (cMo-DC). Vet Immunol Immunopathol. 2007;118:134-9 pubmed
	Wong C, Cheung P, Ip W, Lam C. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007;37:85-96 pubmed
	Droemann D, Rupp J, Goldmann T, Uhlig U, Branscheid D, Vollmer E, et al. Disparate innate immune responses to persistent and acute Chlamydia pneumoniae infection in chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 2007;175:791-7 pubmed
	Fernandez S, Palmer D, Simmons M, Sun P, Bisbing J, McClain S, et al. Potential role for Toll-like receptor 4 in mediating Escherichia coli maltose-binding protein activation of dendritic cells. Infect Immun. 2007;75:1359-63 pubmed
	Shirey K, Jung J, Carlin J. Up-regulation of gamma interferon receptor expression due to Chlamydia-toll-like receptor interaction does not enhance signal transducer and activator of transcription 1 signaling. Infect Immun. 2006;74:6877-84 pubmed
	Chan G, Guilbert L. Ultraviolet-inactivated human cytomegalovirus induces placental syncytiotrophoblast apoptosis in a Toll-like receptor-2 and tumour necrosis factor-alpha dependent manner. J Pathol. 2006;210:111-20 pubmed
	Razonable R, Henault M, Watson H, Paya C. Nystatin induces secretion of interleukin (IL)-1beta, IL-8, and tumor necrosis factor alpha by a toll-like receptor-dependent mechanism. Antimicrob Agents Chemother. 2005;49:3546-9 pubmed
	Hahn C, Schenkein H, Tew J. Endocarditis-associated oral streptococci promote rapid differentiation of monocytes into mature dendritic cells. Infect Immun. 2005;73:5015-21 pubmed
	Cook E, Stahl J, Esnault S, Barney N, Graziano F. Toll-like receptor 2 expression on human conjunctival epithelial cells: a pathway for Staphylococcus aureus involvement in chronic ocular proinflammatory responses. Ann Allergy Asthma Immunol. 2005;94:486-97 pubmed
	Chen D, Texada D, Duggan C, Liang C, Reden T, Kooragayala L, et al. Surface calreticulin mediates muramyl dipeptide-induced apoptosis in RK13 cells. J Biol Chem. 2005;280:22425-36 pubmed
	Hajishengallis G, Tapping R, Martin M, Nawar H, Lyle E, Russell M, et al. Toll-like receptor 2 mediates cellular activation by the B subunits of type II heat-labile enterotoxins. Infect Immun. 2005;73:1343-9 pubmed
	Koller M, Zwölfer B, Steiner G, Smolen J, Scheinecker C. Phenotypic and functional deficiencies of monocyte-derived dendritic cells in systemic lupus erythematosus (SLE) patients. Int Immunol. 2004;16:1595-604 pubmed
	Ueta M, Nochi T, Jang M, Park E, Igarashi O, Hino A, et al. Intracellularly expressed TLR2s and TLR4s contribution to an immunosilent environment at the ocular mucosal epithelium. J Immunol. 2004;173:3337-47 pubmed
	Iwahashi M, Yamamura M, Aita T, Okamoto A, Ueno A, Ogawa N, et al. Expression of Toll-like receptor 2 on CD16+ blood monocytes and synovial tissue macrophages in rheumatoid arthritis. Arthritis Rheum. 2004;50:1457-67 pubmed
	Hajishengallis G, Genco R. Downregulation of the DNA-binding activity of nuclear factor-kappaB p65 subunit in Porphyromonas gingivalis fimbria-induced tolerance. Infect Immun. 2004;72:1188-91 pubmed
	Coleman J, Benach J. The urokinase receptor can be induced by Borrelia burgdorferi through receptors of the innate immune system. Infect Immun. 2003;71:5556-64 pubmed
	Tsunetsugu Yokota Y, Morikawa Y, Isogai M, Kawana Tachikawa A, Odawara T, Nakamura T, et al. Yeast-derived human immunodeficiency virus type 1 p55(gag) virus-like particles activate dendritic cells (DCs) and induce perforin expression in Gag-specific CD8(+) T cells by cross-presentation of DCs. J Virol. 2003;77:10250-9 pubmed
	Kyburz D, Rethage J, Seibl R, Lauener R, Gay R, Carson D, et al. Bacterial peptidoglycans but not CpG oligodeoxynucleotides activate synovial fibroblasts by toll-like receptor signaling. Arthritis Rheum. 2003;48:642-50 pubmed
	Droemann D, Goldmann T, Branscheid D, Clark R, Dalhoff K, Zabel P, et al. Toll-like receptor 2 is expressed by alveolar epithelial cells type II and macrophages in the human lung. Histochem Cell Biol. 2003;119:103-8 pubmed
	Mori Y, Yoshimura A, Ukai T, Lien E, Espevik T, Hara Y. Immunohistochemical localization of Toll-like receptors 2 and 4 in gingival tissue from patients with periodontitis. Oral Microbiol Immunol. 2003;18:54-8 pubmed
	Faure E, Thomas L, Xu H, Medvedev A, Equils O, Arditi M. Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappa B activation. J Immunol. 2001;166:2018-24 pubmed
	Flo T, Halaas O, Lien E, Ryan L, Teti G, Golenbock D, et al. Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide. J Immunol. 2000;164:2064-9 pubmed
	Lien E, Sellati T, Yoshimura A, Flo T, Rawadi G, Finberg R, et al. Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products. J Biol Chem. 1999;274:33419-25 pubmed

product information

Product Type :

Antibody

Product Name :

CD282 (TLR2) Monoclonal Antibody (TL2.1), eBioscience™

Catalog # :

14-9922-80

Quantity :

25 µg

Price :

US 138

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 2 µg/test, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: Assay-Dependent

Species :

Human

Clone :

TL2.1

Isotype :

IgG2a, kappa

Storage :

4° C

Description :

TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 2 µg/test, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: Assay-Dependent

Aliases :

CD282; Ly105; TIL4; Tlr2; TLR-2; toll like receptor 2; toll/interleukin-1 receptor-like protein 4; Toll-like receptor; Toll-like receptor 2; toll-like receptor 2 variant 1; toll-like receptor 2 variant 2; toll-like-receptor 2, exon 1a_; Toll-like-receptor, exon 1b; Toll-like-receptor, exon 1b_; Toll-like-receptor2, exon 1a

more info or order :

Invitrogen product webpage