PCNA Monoclonal Antibody (PC10 (3F81)), eBioscience™ | Invitrogen 14-9910-80 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PCNA Monoclonal Antibody (PC10 (3F81)), eBioscience™

catalog :

14-9910-80

quantity :

25 µg

price :

US 78.25

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PC10 (3F81)

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 2	Baydoun H, Pancewicz J, Bai X, Nicot C. HTLV-I p30 inhibits multiple S phase entry checkpoints, decreases cyclin E-CDK2 interactions and delays cell cycle progression. Mol Cancer. 2010;9:302 pubmed publisher
	McCarthy M, Lin D, Soga T, Adam J, O Callaghan C. Inosine pranobex enhances human NK cell cytotoxicity by inducing metabolic activation and NKG2D ligand expression. Eur J Immunol. 2020;50:130-137 pubmed publisher
	Lafontant P, Burns A, Grivas J, Lesch M, Lala T, Reuter S, et al. The giant danio (D. aequipinnatus) as a model of cardiac remodeling and regeneration. Anat Rec (Hoboken). 2012;295:234-48 pubmed publisher
	Hall P, Levison D, Woods A, Yu C, Kellock D, Watkins J, et al. Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol. 1990;162:285-94 pubmed
	Waseem N, Lane D. Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form. J Cell Sci. 1990;96 ( Pt 1):121-9 pubmed
	Landberg G, Roos G. Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analysis. Cancer Res. 1991;51:4570-4 pubmed

product information

Product Type :

Antibody

Product Name :

PCNA Monoclonal Antibody (PC10 (3F81)), eBioscience™

Catalog # :

14-9910-80

Quantity :

25 µg

Price :

US 78.25

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Flow Cytometry: 0.25 µg/test, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunohistochemistry: Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: 1 µg/mL

Species :

Human, Mouse, Rat

Clone :

PC10 (3F81)

Isotype :

IgG2a, kappa

Storage :

4° C

Description :

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.25 µg/test, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunohistochemistry: Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: 1 µg/mL

Aliases :

ATLD2; cb16; cyclin; DNA polymerase delta auxiliary protein; etID36690.10; fa28e03; fb36g03; HGCN8729; hypothetical protein LOC515499; LOC100339381; MGC8367; PCNA; pcna protein; Pcna/cyclin; PCNAR; POL30; proliferating cell nuclear antigen; proliferating cell nuclear antigen-like; wu:fa28e03; wu:fb36g03; YBR0811; YBR088C

more info or order :

Invitrogen product webpage