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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Nestin Monoclonal Antibody (10C2), eBioscience™
catalog :
14-9843-82
quantity :
100µg
price :
US 345
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
10C2
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 7
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; human; 1:50; loading ...; fig 1g
  • immunocytochemistry; human; 1:50; fig 2e
Abe S, Yamaguchi S, Sato Y, Harada K. Sphere-Derived Multipotent Progenitor Cells Obtained From Human Oral Mucosa Are Enriched in Neural Crest Cells. Stem Cells Transl Med. 2016;5:117-28 pubmed publisher
Bhanu N, Sidoli S, Garcia B. Histone modification profiling reveals differential signatures associated with human embryonic stem cell self-renewal and differentiation. Proteomics. 2016;16:448-58 pubmed publisher
Mullins C, Schneider B, Stockhammer F, Krohn M, Classen C, Linnebacher M. Establishment and characterization of primary glioblastoma cell lines from fresh and frozen material: a detailed comparison. PLoS ONE. 2013;8:e71070 pubmed publisher
Buck J, Garde S, Kohl C, Knaup Gregori P. Towards a comprehensive electronic patient record to support an innovative individual care concept for premature infants using the openEHR approach. Int J Med Inform. 2009;78:521-31 pubmed publisher
Messam C, Ding S, Haydon P. Functional differentiation of human brain progenitor cells. Neuron Glia Biol. 2006;2:187-98 pubmed publisher
Linning K, Tai M, Madhukar B, Chang C, Reed D, Ferber S, et al. Redox-mediated enrichment of self-renewing adult human pancreatic cells that possess endocrine differentiation potential. Pancreas. 2004;29:e64-76 pubmed
Messam C, Hou J, Major E. Coexpression of nestin in neural and glial cells in the developing human CNS defined by a human-specific anti-nestin antibody. Exp Neurol. 2000;161:585-96 pubmed
product information
Product Type :
Antibody
Product Name :
Nestin Monoclonal Antibody (10C2), eBioscience™
Catalog # :
14-9843-82
Quantity :
100µg
Price :
US 345
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: Assay-Dependent, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunohistochemistry (Frozen): Assay-Dependent, Immunohistochemistry (Paraffin): Assay-Dependent, Western Blot: 1-5 µg/mL
Species :
Human
Clone :
10C2
Isotype :
IgG1
Storage :
4° C
Description :
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: Assay-Dependent, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunohistochemistry (Frozen): Assay-Dependent, Immunohistochemistry (Paraffin): Assay-Dependent, Western Blot: 1-5 µg/mL
Aliases :
AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; NES; Nestin
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA