product summary
Loading...
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Vinculin Monoclonal Antibody (7F9), eBioscience™
catalog :
14-9777-82
quantity :
100µg
price :
US 305
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
7F9
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 5
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; loading ...; fig 7c
Xie X, Almuzzaini B, Drou N, Kremb S, Yousif A, Farrants A, et al. β-Actin-dependent global chromatin organization and gene expression programs control cellular identity. FASEB J. 2018;32:1296-1314 pubmed publisher
Davis E, Kim J, Menasche B, Sheppard J, Liu X, Tan A, et al. Comparative Haploid Genetic Screens Reveal Divergent Pathways in the Biogenesis and Trafficking of Glycophosphatidylinositol-Anchored Proteins. Cell Rep. 2015;11:1727-36 pubmed publisher
Peng X, Cuff L, Lawton C, DeMali K. Vinculin regulates cell-surface E-cadherin expression by binding to beta-catenin. J Cell Sci. 2010;123:567-77 pubmed publisher
Geiger B, Tokuyasu K, Dutton A, Singer S. Vinculin, an intracellular protein localized at specialized sites where microfilament bundles terminate at cell membranes. Proc Natl Acad Sci U S A. 1980;77:4127-31 pubmed
Gilmore A, Jackson P, Waites G, Critchley D. Further characterisation of the talin-binding site in the cytoskeletal protein vinculin. J Cell Sci. 1992;103 ( Pt 3):719-31 pubmed
product information
Product Type :
Antibody
Product Name :
Vinculin Monoclonal Antibody (7F9), eBioscience™
Catalog # :
14-9777-82
Quantity :
100µg
Price :
US 305
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human, Mouse, Rat
Applications :
Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunohistochemistry (Frozen): 5 µg/mL, Immunohistochemistry (Paraffin): Assay-Dependent, Western Blot: 1-5 µg/mL
Species :
Human, Mouse, Rat
Clone :
7F9
Isotype :
IgG1, kappa
Storage :
4° C
Description :
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunohistochemistry (Frozen): 5 µg/mL, Immunohistochemistry (Paraffin): Assay-Dependent, Western Blot: 1-5 µg/mL
Aliases :
9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; epididymis luminal protein 114; HEL114; Metavinculin; metavinculin {ECO:0000250; MV; MVCL; RP11-178G16.3; UniProtKB:P18206}; VCL; vcl {ECO:0000250; vinc; VINC1; Vinculin; vinculin {ECO:0000250
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA