Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), eBioscience | Invitrogen 14-9760-80 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), eBioscience

catalog :

14-9760-80

quantity :

25 µg

price :

US 105

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1A4

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 38

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig 2b	Ortiz Otero N, Marshall J, Lash B, King M. Chemotherapy-induced release of circulating-tumor cells into the bloodstream in collective migration units with cancer-associated fibroblasts in metastatic cancer patients. BMC Cancer. 2020;20:873 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 4d	Yazawa K, Nakamura F, Masukawa D, Sato S, Hiroshima Y, Yabushita Y, et al. Low Incidence of High-Grade Pancreatic Intraepithelial Neoplasia Lesions in a Crmp4 Gene-Deficient Mouse Model of Pancreatic Cancer. Transl Oncol. 2020;13:100746 pubmed publisher
western blot; mouse; 5 ug/ml; loading ...; fig 7	Chauhan P, Dash D, Singh R. Intranasal Curcumin Inhibits Pulmonary Fibrosis by Modulating Matrix Metalloproteinase-9 (MMP-9) in Ovalbumin-Induced Chronic Asthma. Inflammation. 2017;40:248-258 pubmed publisher
	El Sherbiny M, Atef H, Eladl M, Mohamed A, El Shafey M, Ali H, et al. Leflunomide Induces Dose-Dependent Lung Injury in Mice via Stimulating Vimentin and NLRP3 Inflammasome Production. Front Pharmacol. 2021;12:631216 pubmed publisher
	Zhang L, He J, Wang J, Liu J, Chen Z, Deng B, et al. Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy. Cell Death Dis. 2021;12:470 pubmed publisher
	Prados A, Onder L, Cheng H, Mörbe U, Lütge M, Gil Cruz C, et al. Fibroblastic reticular cell lineage convergence in Peyer's patches governs intestinal immunity. Nat Immunol. 2021;22:510-519 pubmed publisher
	Panera N, Meroni M, Longo M, Crudele A, Valenti L, Bellacchio E, et al. The KLB rs17618244 gene variant is associated with fibrosing MAFLD by promoting hepatic stellate cell activation. EBioMedicine. 2021;65:103249 pubmed publisher
	Xie S, Shan X, Yau V, Zhang J, Zhang X, Yan Y, et al. Hyperion imaging system reveals heterogeneous tumor microenvironment of oral squamous cell carcinoma patients at T1N0M0 stage. Ann Transl Med. 2020;8:1513 pubmed publisher
	Lu S, Jolly A, Strand K, Dubner A, Mutryn M, Moulton K, et al. Smooth muscle-derived progenitor cell myofibroblast differentiation through KLF4 downregulation promotes arterial remodeling and fibrosis. JCI Insight. 2020;5: pubmed publisher
	Gilles G, McCulloch A, Brakebusch C, Herum K. Maintaining resting cardiac fibroblasts in vitro by disrupting mechanotransduction. PLoS ONE. 2020;15:e0241390 pubmed publisher
	Gorchs L, Ahmed S, Mayer C, Knauf A, Fernández Moro C, Svensson M, et al. The vitamin D analogue calcipotriol promotes an anti-tumorigenic phenotype of human pancreatic CAFs but reduces T cell mediated immunity. Sci Rep. 2020;10:17444 pubmed publisher
	Kamand M, Ilieva M, Louise Forsberg S, Thomassen M, Meyer M, Fex Svenningsen A, et al. Derivation of induced pluripotent stem cells (SDUKIi003-A) from a 20-year-old male patient diagnosed with Asperger syndrome. Stem Cell Res. 2020;48:101974 pubmed publisher
	Bersini S, Schulte R, Huang L, Tsai H, Hetzer M. Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome. elife. 2020;9: pubmed publisher
	Suess P, Smith S, Morrissey J. Platelet polyphosphate induces fibroblast chemotaxis and myofibroblast differentiation. J Thromb Haemost. 2020;18:3043-3052 pubmed publisher
	Xie B, Lu C, Chen C, Zhou J, Deng Z. miR-135a Alleviates Silica-Induced Pulmonary Fibrosis by Targeting NF-κB/Inflammatory Signaling Pathway. Mediators Inflamm. 2020;2020:1231243 pubmed publisher
	Gonzalez B, Perez Nevarez M, Mirza A, Perez M, Lin Y, Hsu C, et al. Physiologically Relevant Fluid-Induced Oscillatory Shear Stress Stimulation of Mesenchymal Stem Cells Enhances the Engineered Valve Matrix Phenotype. Front Cardiovasc Med. 2020;7:69 pubmed publisher
	Somarathna M, Isayeva Waldrop T, Al Balas A, Guo L, Lee T. A Novel Model of Balloon Angioplasty Injury in Rat Arteriovenous Fistula. J Vasc Res. 2020;57:223-235 pubmed publisher
	Polley A, Khanam R, Sengupta A, Chakraborty S. Asporin Reduces Adult Aortic Valve Interstitial Cell Mineralization Induced by Osteogenic Media and Wnt Signaling Manipulation In Vitro. Int J Cell Biol. 2020;2020:2045969 pubmed publisher
	Cozart M, Phelan K, Wu H, Mu S, Birnbaumer L, Rusch N, et al. Vascular smooth muscle TRPC3 channels facilitate the inverse hemodynamic response during status epilepticus. Sci Rep. 2020;10:812 pubmed publisher
	Wang T, Xie S, Luo R, Shi L, Bai P, Wang X, et al. Two novel TSC2 mutations in renal epithelioid angiomyolipoma sensitive to everolimus. Cancer Biol Ther. 2020;21:4-11 pubmed publisher
	Martinez L, Robila V, Clark N, Du W, Idowu M, Rutkowski M, et al. Regulatory T Cells Control the Switch From in situ to Invasive Breast Cancer. Front Immunol. 2019;10:1942 pubmed publisher
	Charbonnier B, Baradaran A, Sato D, Alghamdi O, Zhang Z, Zhang Y, et al. Material-Induced Venosome-Supported Bone Tubes. Adv Sci (Weinh). 2019;6:1900844 pubmed publisher
	Lin F, Zeng Z, Song Y, Li L, Wu Z, Zhang X, et al. YBX-1 mediated sorting of miR-133 into hypoxia/reoxygenation-induced EPC-derived exosomes to increase fibroblast angiogenesis and MEndoT. Stem Cell Res Ther. 2019;10:263 pubmed publisher
	Okano S, Yasui A, Kanno S, Satoh K, Igarashi M, Nakajima O. Karyopherin Alpha 2-Expressing Pancreatic Duct Glands and Intra-Islet Ducts in Aged Diabetic C414A-Mutant-CRY1 Transgenic Mice. J Diabetes Res. 2019;2019:7234549 pubmed publisher
	Lin J, Izar B, Wang S, Yapp C, Mei S, Shah P, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. elife. 2018;7: pubmed publisher
	Foo K, Lehtinen M, Leung C, Lian X, Xu J, Keung W, et al. Human ISL1+ Ventricular Progenitors Self-Assemble into an In Vivo Functional Heart Patch and Preserve Cardiac Function Post Infarction. Mol Ther. 2018;26:1644-1659 pubmed publisher
	Kaur H, Carvalho J, Looso M, Singh P, Chennupati R, Preussner J, et al. Single-cell profiling reveals heterogeneity and functional patterning of GPCR expression in the vascular system. Nat Commun. 2017;8:15700 pubmed publisher
	Young H, Rowling E, Bugatti M, Giurisato E, Luheshi N, Arozarena I, et al. An adaptive signaling network in melanoma inflammatory niches confers tolerance to MAPK signaling inhibition. J Exp Med. 2017;214:1691-1710 pubmed publisher
	Bakhtyar N, Jeschke M, Mainville L, Herer E, Amini Nik S. Acellular Gelatinous Material of Human Umbilical Cord Enhances Wound Healing: A Candidate Remedy for Deficient Wound Healing. Front Physiol. 2017;8:200 pubmed publisher
	Chen Q, Zhang H, Liu Y, Adams S, Eilken H, Stehling M, et al. Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells. Nat Commun. 2016;7:12422 pubmed publisher
	Chaponnier C, Gabbiani G. Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle. F1000Res. 2016;5:416 pubmed publisher
	MercuŢ R, Ciurea M, Margaritescu C, Popescu S, Crăiţoiu M, Cotoi O, et al. Expression of p53, D2-40 and ?-smooth muscle actin in different histological subtypes of facial basal cell carcinoma. Rom J Morphol Embryol. 2014;55:263-72 pubmed
	Fujiwara K, Hasegawa K, Ohkumo T, Miyoshi H, Tseng Y, Yoshikawa K. Necdin controls proliferation of white adipocyte progenitor cells. PLoS ONE. 2012;7:e30948 pubmed publisher
	Holtz M, Misra R. Serum response factor is required for cell contact maintenance but dispensable for proliferation in visceral yolk sac endothelium. BMC Dev Biol. 2011;11:18 pubmed publisher
	Daley E, Emson C, Guignabert C, de Waal Malefyt R, Louten J, Kurup V, et al. Pulmonary arterial remodeling induced by a Th2 immune response. J Exp Med. 2008;205:361-72 pubmed publisher
	Heissig B, Rafii S, Akiyama H, Ohki Y, Sato Y, Rafael T, et al. Low-dose irradiation promotes tissue revascularization through VEGF release from mast cells and MMP-9-mediated progenitor cell mobilization. J Exp Med. 2005;202:739-50 pubmed
	Skalli O, Ropraz P, Trzeciak A, Benzonana G, Gillessen D, Gabbiani G. A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol. 1986;103:2787-96 pubmed
	Roholl P, Elbers H, Prinsen I, Claessens J, van Unnik J. Distribution of actin isoforms in sarcomas: an immunohistochemical study. Hum Pathol. 1990;21:1269-74 pubmed

product information

Product Type :

Antibody

Product Name :

Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), eBioscience

Catalog # :

14-9760-80

Quantity :

25 µg

Price :

US 105

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Flow Cytometry: Assay-Dependent, Immunocytochemistry: 1 µg/mL, Immunohistochemistry (Frozen): 1 µg/mL, Immunohistochemistry (Paraffin): Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: 1:100

Species :

Human, Mouse, Rat

Clone :

1A4

Isotype :

IgG2a, kappa

Storage :

4° C

Description :

Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.

Format :

Liquid

Applications w/Dilutions :

Aliases :

0610041G09Rik; AAT6; ACT-4; acta2; acta2.L; acta2.S; actin; Actin Alpha 2 Smooth Muscle Aorta; actin alpha 2, smooth muscle; Actin Vascular Smooth Muscle; actin, alpha 2, smooth muscle, aorta; actin, alpha 2, smooth muscle, aorta L homeolog; actin, alpha 2, smooth muscle, aorta S homeolog; actin, alpha, vascular smooth muscle; actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; ACTSA; ACTVS; alpha actin 2; Alpha Cardiac Actin; alpha smooth muscle actin; alpha-actin; Alpha-actin-2; alpha-cardiac actin; alphaSMA; alpha-SM-actin; alpha-smooth muscle actin; Aortic Smooth Muscle; ASMA; a-sma; cell growth-inhibiting gene 46 protein; GIG46; Growth Inhibiting Gene 46; hypothetical protein LOC515610; LOW QUALITY PROTEIN: actin, aortic smooth muscle; mymy5; SM actin; sma; SMactin; sm-actin; SMalphaA; smooth muscle; smooth muscle alpha-actin; vascular smooth muscle alpha-actin; wu:fb63d03; XELAEV_18034370mg

more info or order :

Invitrogen product webpage