Perforin Monoclonal Antibody (eBioOMAK-D), eBioscience™ | Invitrogen 14-9392-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Perforin Monoclonal Antibody (eBioOMAK-D), eBioscience™

catalog :

14-9392-82

quantity :

100 µg

price :

349 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

eBioOMAK-D

reactivity :

mouse

application :

western blot, flow cytometry

more info or order :

Invitrogen product webpage

citations: 51

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 5c	Xiao F, Ai G, Yan W, Wan X, Luo X, Ning Q. Intrahepatic recruitment of cytotoxic NK cells contributes to autoimmune hepatitis progression. Cell Immunol. 2018;327:13-20 pubmed publisher
flow cytometry; mouse; loading ...; fig 4j	FÃ¶rster M, Boora R, Petrov J, Fodil N, Albanese I, Kim J, et al. A role for the histone H2A deubiquitinase MYSM1 in maintenance of CD8+ T cells. Immunology. 2017;151:110-121 pubmed publisher
flow cytometry; mouse; 1:50; loading ...; tbl s2	Leong Y, Chen Y, Ong H, Wu D, Man K, Deléage C, et al. CXCR5(+) follicular cytotoxic T cells control viral infection in B cell follicles. Nat Immunol. 2016;17:1187-96 pubmed publisher
flow cytometry; mouse	Murphy K, Erickson J, Johnson C, Seiler C, Bedi J, Hu P, et al. CD8+ T cell-independent tumor regression induced by Fc-OX40L and therapeutic vaccination in a mouse model of glioma. J Immunol. 2014;192:224-33 pubmed publisher
flow cytometry; mouse	Jansen A, Hall L, Clare S, Goulding D, Holt K, Grant A, et al. A Salmonella Typhimurium-Typhi genomic chimera: a model to study Vi polysaccharide capsule function in vivo. PLoS Pathog. 2011;7:e1002131 pubmed publisher
flow cytometry; mouse; fig 3	Zou Y, Chen T, Han M, Wang H, Yan W, Song G, et al. Increased killing of liver NK cells by Fas/Fas ligand and NKG2D/NKG2D ligand contributes to hepatocyte necrosis in virus-induced liver failure. J Immunol. 2010;184:466-75 pubmed publisher
flow cytometry; mouse	Saito K, Torii M, Ma N, Tsuchiya T, Wang L, Hori T, et al. Differential regulatory function of resting and preactivated allergen-specific CD4+ CD25+ regulatory T cells in Th2-type airway inflammation. J Immunol. 2008;181:6889-97 pubmed
flow cytometry; mouse; fig 3	Badovinac V, Harty J. Manipulating the rate of memory CD8+ T cell generation after acute infection. J Immunol. 2007;179:53-63 pubmed
flow cytometry; mouse; fig 7	Love V, Grabie N, Duramad P, Stavrakis G, Sharpe A, Lichtman A. CTLA-4 ablation and interleukin-12 driven differentiation synergistically augment cardiac pathogenicity of cytotoxic T lymphocytes. Circ Res. 2007;101:248-57 pubmed
	Cai D, Li J, Liu D, Hong S, Qiao Q, Sun Q, et al. Tumor-expressed B7-H3 mediates the inhibition of antitumor T-cell functions in ovarian cancer insensitive to PD-1 blockade therapy. Cell Mol Immunol. 2019;: pubmed publisher
	Wang F, Meng M, Mo B, Yang Y, Ji Y, Huang P, et al. Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function. Nat Commun. 2018;9:4874 pubmed publisher
	Tosello Boari J, Araujo Furlan C, Fiocca Vernengo F, Rodríguez C, Ramello M, Amezcua Vesely M, et al. IL-17RA-Signaling Modulates CD8+ T Cell Survival and Exhaustion During Trypanosoma cruzi Infection. Front Immunol. 2018;9:2347 pubmed publisher
	Ekiz H, Lai S, Gundlapalli H, Haroun F, Williams M, Welm A. Inhibition of RON kinase potentiates anti-CTLA-4 immunotherapy to shrink breast tumors and prevent metastatic outgrowth. Oncoimmunology. 2018;7:e1480286 pubmed publisher
	Kim S, Lee S, Park H, Lee S, Im W, Kim Y, et al. Anti-cancer activity of Angelica gigas by increasing immune response and stimulating natural killer and natural killer T cells. BMC Complement Altern Med. 2018;18:218 pubmed publisher
	Seo N, Shirakura Y, Tahara Y, Momose F, Harada N, Ikeda H, et al. Activated CD8+ T cell extracellular vesicles prevent tumour progression by targeting of lesional mesenchymal cells. Nat Commun. 2018;9:435 pubmed publisher
	Klein Hessling S, Muhammad K, Klein M, Pusch T, Rudolf R, Flöter J, et al. NFATc1 controls the cytotoxicity of CD8+ T cells. Nat Commun. 2017;8:511 pubmed publisher
	Kim K, Bae S, Capece T, Nedelkovska H, de Rubio R, Smrcka A, et al. Targeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment. Nat Commun. 2017;8:15365 pubmed publisher
	Dai K, Huang L, Huang Y, Chen Z, Yang L, Jiang Y. 1810011o10 Rik Inhibits the Antitumor Effect of Intratumoral CD8+ T Cells through Suppression of Notch2 Pathway in a Murine Hepatocellular Carcinoma Model. Front Immunol. 2017;8:320 pubmed publisher
	Li M, Li Z, Yao Y, Jin W, Wood K, Liu Q, et al. Astrocyte-derived interleukin-15 exacerbates ischemic brain injury via propagation of cellular immunity. Proc Natl Acad Sci U S A. 2017;114:E396-E405 pubmed publisher
	Krneta T, Gillgrass A, Chew M, Ashkar A. The breast tumor microenvironment alters the phenotype and function of natural killer cells. Cell Mol Immunol. 2016;13:628-39 pubmed publisher
	Hou H, Liu W, Wu S, Lu Y, Peng J, Zhu Y, et al. Tim-3 negatively mediates natural killer cell function in LPS-induced endotoxic shock. PLoS ONE. 2014;9:e110585 pubmed publisher
	Lee S, Park H, Park S, Kim N, Hong S. Immunomodulatory effect of poly-?-glutamic acid derived from Bacillus subtilis on natural killer dendritic cells. Biochem Biophys Res Commun. 2014;443:413-21 pubmed publisher
	Takigawa T, Miyazaki H, Kinoshita M, Kawarabayashi N, Nishiyama K, Hatsuse K, et al. Glucocorticoid receptor-dependent immunomodulatory effect of ursodeoxycholic acid on liver lymphocytes in mice. Am J Physiol Gastrointest Liver Physiol. 2013;305:G427-38 pubmed publisher
	Liu R, Engels B, Schreiber K, Ciszewski C, Schietinger A, Schreiber H, et al. IL-15 in tumor microenvironment causes rejection of large established tumors by T cells in a noncognate T cell receptor-dependent manner. Proc Natl Acad Sci U S A. 2013;110:8158-63 pubmed publisher
	Hildemann S, Eberlein J, Davenport B, Nguyen T, Victorino F, Homann D. High efficiency of antiviral CD4(+) killer T cells. PLoS ONE. 2013;8:e60420 pubmed publisher
	Peng H, Sun R, Tang L, Wei H, Tian Z. CD62L is critical for maturation and accumulation of murine hepatic NK cells in response to viral infection. J Immunol. 2013;190:4255-62 pubmed publisher
	Ali R, Naqvi R, Kumar S, Bhat A, Rao D. Multiple antigen peptide containing B and T cell epitopes of F1 antigen of Yersinia pestis showed enhanced Th1 immune response in murine model. Scand J Immunol. 2013;77:361-71 pubmed publisher
	Ma D, Suthar M, Kasahara S, Gale M, Clark E. CD22 is required for protection against West Nile virus Infection. J Virol. 2013;87:3361-75 pubmed publisher
	Bao Y, Zheng J, Han C, Jin J, Han H, Liu Y, et al. Tyrosine kinase Btk is required for NK cell activation. J Biol Chem. 2012;287:23769-78 pubmed publisher
	Wu L, Yun Z, Tagawa T, Rey McIntyre K, de Perrot M. CTLA-4 blockade expands infiltrating T cells and inhibits cancer cell repopulation during the intervals of chemotherapy in murine mesothelioma. Mol Cancer Ther. 2012;11:1809-19 pubmed publisher
	Wu X, Chen Y, Wei H, Sun R, Tian Z. Development of murine hepatic NK cells during ontogeny: comparison with spleen NK cells. Clin Dev Immunol. 2012;2012:759765 pubmed publisher
	Yamashita J, Iwamura C, Mitsumori K, Hosokawa H, Sasaki T, Takahashi M, et al. Murine Schnurri-2 controls natural killer cell function and lymphoma development. Leuk Lymphoma. 2012;53:479-86 pubmed publisher
	Kitajima M, Ito T, Tumes D, Endo Y, Onodera A, Hashimoto K, et al. Memory type 2 helper T cells induce long-lasting antitumor immunity by activating natural killer cells. Cancer Res. 2011;71:4790-8 pubmed publisher
	Narumi K, Udagawa T, Kondoh A, Kobayashi A, Hara H, Ikarashi Y, et al. In vivo delivery of interferon-? gene enhances tumor immunity and suppresses immunotolerance in reconstituted lymphopenic hosts. Gene Ther. 2012;19:34-48 pubmed publisher
	Tosiek M, Gruber A, Bader S, Mauel S, Hoymann H, Prettin S, et al. CD4+CD25+Foxp3+ regulatory T cells are dispensable for controlling CD8+ T cell-mediated lung inflammation. J Immunol. 2011;186:6106-18 pubmed publisher
	Xie Y, Akpinarli A, Maris C, Hipkiss E, Lane M, Kwon E, et al. Naive tumor-specific CD4(+) T cells differentiated in vivo eradicate established melanoma. J Exp Med. 2010;207:651-67 pubmed publisher
	Cavassani K, Carson W, Moreira A, Wen H, Schaller M, Ishii M, et al. The post sepsis-induced expansion and enhanced function of regulatory T cells create an environment to potentiate tumor growth. Blood. 2010;115:4403-11 pubmed publisher
	Lowe D, Shearer M, Jumper C, Bright R, Kennedy R. Tumor immunity against a simian virus 40 oncoprotein requires CD8+ T lymphocytes in the effector immune phase. J Virol. 2010;84:883-93 pubmed publisher
	Thapa M, Carr D. CXCR3 deficiency increases susceptibility to genital herpes simplex virus type 2 infection: Uncoupling of CD8+ T-cell effector function but not migration. J Virol. 2009;83:9486-501 pubmed publisher
	Xie X, Stadnisky M, Brown M. MHC class I Dk locus and Ly49G2+ NK cells confer H-2k resistance to murine cytomegalovirus. J Immunol. 2009;182:7163-71 pubmed publisher
	Jin G, Hirano T, Murakami M. Combination treatment with IL-2 and anti-IL-2 mAbs reduces tumor metastasis via NK cell activation. Int Immunol. 2008;20:783-9 pubmed publisher
	Murakami T, Sato A, Chun N, Hara M, Naito Y, Kobayashi Y, et al. Transcriptional modulation using HDACi depsipeptide promotes immune cell-mediated tumor destruction of murine B16 melanoma. J Invest Dermatol. 2008;128:1506-16 pubmed publisher
	Strid J, Roberts S, Filler R, Lewis J, Kwong B, Schpero W, et al. Acute upregulation of an NKG2D ligand promotes rapid reorganization of a local immune compartment with pleiotropic effects on carcinogenesis. Nat Immunol. 2008;9:146-54 pubmed publisher
	Hutchens M, Luker K, Sottile P, Sonstein J, Lukacs N, Nunez G, et al. TLR3 increases disease morbidity and mortality from vaccinia infection. J Immunol. 2008;180:483-91 pubmed
	Gu T, Kilinc M, Egilmez N. Transient activation of tumor-associated T-effector/memory cells promotes tumor eradication via NK-cell recruitment: minimal role for long-term T-cell immunity in cure of metastatic disease. Cancer Immunol Immunother. 2008;57:997-1005 pubmed
	Fehniger T, Cai S, Cao X, Bredemeyer A, Presti R, French A, et al. Acquisition of murine NK cell cytotoxicity requires the translation of a pre-existing pool of granzyme B and perforin mRNAs. Immunity. 2007;26:798-811 pubmed
	Chaitra M, Shaila M, Nayak R. Evaluation of T-cell responses to peptides with MHC class I-binding motifs derived from PE_PGRS 33 protein of Mycobacterium tuberculosis. J Med Microbiol. 2007;56:466-74 pubmed
	Slifka M, Rodriguez F, Whitton J. Rapid on/off cycling of cytokine production by virus-specific CD8+ T cells. Nature. 1999;401:76-9 pubmed
	Opferman J, Ober B, Ashton Rickardt P. Linear differentiation of cytotoxic effectors into memory T lymphocytes. Science. 1999;283:1745-8 pubmed
	Liu C, Walsh C, Young J. Perforin: structure and function. Immunol Today. 1995;16:194-201 pubmed
	Walsh C, Matloubian M, Liu C, Ueda R, Kurahara C, Christensen J, et al. Immune function in mice lacking the perforin gene. Proc Natl Acad Sci U S A. 1994;91:10854-8 pubmed

product information

Product Type :

Antibody

Product Name :

Perforin Monoclonal Antibody (eBioOMAK-D), eBioscience™

Catalog # :

14-9392-82

Quantity :

100 µg

Price :

349 USD

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rat

Reactivity :

Mouse

Applications :

Western Blot: 2 µg/mL

Species :

Mouse

Clone :

eBioOMAK-D

Isotype :

IgG2a, kappa

Storage :

4° C

Description :

Perforin is one of the major cytolytic proteins of cytolytic granules. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes. Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.

Format :

Liquid

Applications w/Dilutions :

Western Blot: 2 µg/mL

Aliases :

Cyta; Cytolysin; FLH2; HPLH2; lymphocyte pore forming protein; lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; P1; perforin 1; perforin 1 (pore forming protein); Perforin1; perforin-1; Pfn; PFN1; Pfp; pore forming protein; pore-forming protein; Prf1; Prf-1; RATCYTA; RP11-710A11.3

more info or order :

Invitrogen product webpage