EEA1 Monoclonal Antibody (1G11), eBioscience | Invitrogen 14-9114-80 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

EEA1 Monoclonal Antibody (1G11), eBioscience

catalog :

14-9114-80

quantity :

25 ug

price :

US 177.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1G11

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:200; loading ...; fig 3d	Martínez Fábregas J, Wilmes S, Wang L, Hafer M, Pohler E, Lokau J, et al. Kinetics of cytokine receptor trafficking determine signaling and functional selectivity. elife. 2019;8: pubmed publisher
immunocytochemistry; human; 1:500; loading ...; fig 7s1	Achberger K, Probst C, Haderspeck J, Bolz S, Rogal J, Chuchuy J, et al. Merging organoid and organ-on-a-chip technology to generate complex multi-layer tissue models in a human retina-on-a-chip platform. elife. 2019;8: pubmed publisher
immunocytochemistry; human; loading ...; fig 3f	Burr M, Sparbier C, Chan Y, Williamson J, Woods K, Beavis P, et al. CMTM6 maintains the expression of PD-L1 and regulates anti-tumour immunity. Nature. 2017;549:101-105 pubmed publisher
	Ding L, Chen X, Zhang W, Dai X, Guo H, Pan X, et al. Canagliflozin primes antitumor immunity by triggering PD-L1 degradation in endocytic recycling. J Clin Invest. 2023;133: pubmed publisher
	Betriu N, Andreeva A, Semino C. Erlotinib Promotes Ligand-Induced EGFR Degradation in 3D but Not 2D Cultures of Pancreatic Ductal Adenocarcinoma Cells. Cancers (Basel). 2021;13: pubmed publisher
	Sofou K, Meier K, Sanderson L, Kaminski D, Montoliu Gaya L, Samuelsson E, et al. Bi-allelic VPS16 variants limit HOPS/CORVET levels and cause a mucopolysaccharidosis-like disease. EMBO Mol Med. 2021;13:e13376 pubmed publisher
	Selak S, Braun J, Fritzler M. Characterization of early endosome antigen 1 in neural tissues. Biochem Biophys Res Commun. 2004;323:1334-42 pubmed
	Simonsen A, Gaullier J, D Arrigo A, Stenmark H. The Rab5 effector EEA1 interacts directly with syntaxin-6. J Biol Chem. 1999;274:28857-60 pubmed
	Selak S, Chan E, Schoenroth L, Senecal J, Fritzler M. Early endosome antigen. 1: An autoantigen associated with neurological diseases. J Investig Med. 1999;47:311-8 pubmed
	Mu F, Callaghan J, Steele Mortimer O, Stenmark H, Parton R, Campbell P, et al. EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine "fingers" and contains a calmodulin-binding IQ motif. J Biol Chem. 1995;270:13503-11 pubmed

product information

Product Type :

Antibody

Product Name :

EEA1 Monoclonal Antibody (1G11), eBioscience

Catalog # :

14-9114-80

Quantity :

25 ug

Price :

US 177.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: Assay-Dependent, Immunocytochemistry: 1 ug/mL, Immunohistochemistry (Paraffin): Assay-Dependent, Western Blot: Assay-Dependent

Species :

Human, Mouse, Rat

Clone :

1G11

Isotype :

IgG1, kappa

Storage :

4 C

Description :

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc-finger-like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl-terminal domains of EEA1. The carboxyl-terminal zinc-finger-like-domain is conserved in several other non-nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc-binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3-kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-Dependent, Immunocytochemistry: 1 ug/mL, Immunohistochemistry (Paraffin): Assay-Dependent, Western Blot: Assay-Dependent

Aliases :

A430109M19Rik; B230358H09Rik; Early endosome antigen 1; early endosome antigen 1, 162kD; early endosome-associated protein; EEA1; Endosome-associated protein p162; MST105; MSTP105; ZFYVE2; Zinc finger FYVE domain-containing protein 2

more info or order :

Invitrogen product webpage