Granzyme B Monoclonal Antibody (496B), eBioscience | Invitrogen 14-8889-82 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Granzyme B Monoclonal Antibody (496B), eBioscience

catalog :

14-8889-82

quantity :

100 ug

price :

US 440.00

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

496B

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

citations: 7

Reference
Galat Y, Du Y, Perepitchka M, Li X, Balyasnikova I, Tse W, et al. In vitro vascular differentiation system efficiently produces natural killer cells for cancer immunotherapies. Oncoimmunology. 2023;12:2240670 pubmed publisher
Rasi V, Hameed O, Matthey P, Bera S, Grandgenett D, Salentinig S, et al. Improved Purification of Human Granzyme A/B and Granulysin Using a Mammalian Expression System. Front Immunol. 2022;13:830290 pubmed publisher
Lee J, Lee S, Gu N, Jeong S, Byeon J, Jeong D, et al. Canine Natural Killer Cell-Derived Exosomes Exhibit Antitumor Activity in a Mouse Model of Canine Mammary Tumor. Biomed Res Int. 2021;2021:6690704 pubmed publisher
Goodridge J, Jacobs B, Saetersmoen M, Clement D, Hammer Q, Clancy T, et al. Remodeling of secretory lysosomes during education tunes functional potential in NK cells. Nat Commun. 2019;10:514 pubmed publisher
Smyth M, Trapani J. Granzymes: exogenous proteinases that induce target cell apoptosis. Immunol Today. 1995;16:202-6 pubmed
Trinchieri G, Perussia B. Human natural killer cells: biologic and pathologic aspects. Lab Invest. 1984;50:489-513 pubmed
Hameed A, Truong L, Price V, Kruhenbuhl O, Tschopp J. Immunohistochemical localization of granzyme B antigen in cytotoxic cells in human tissues. Am J Pathol. 1991;138:1069-75 pubmed

product information

Product Type :

Antibody

Product Name :

Granzyme B Monoclonal Antibody (496B), eBioscience

Catalog # :

14-8889-82

Quantity :

100 ug

Price :

US 440.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rat

Reactivity :

Human

Applications :

Immunocytochemistry: Assay-Dependent, Immunohistochemistry (Paraffin): 5 ug/mL, Western Blot: 1 ug/mL

Species :

Human

Clone :

496B

Isotype :

IgG2a, kappa

Storage :

4 C

Description :

Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: Assay-Dependent, Immunohistochemistry (Paraffin): 5 ug/mL, Western Blot: 1 ug/mL

Aliases :

AI553453; C11; Cathepsin G-like 1; CCP1; CCP-1/C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; Ctla1; Ctla-1; CTSGL1; cytotoxic cell protease 1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin; fragmentin 2; fragmentin-2; GLP I; GLP III; GLP-1; granzyme 2; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); granzyme B(G,H); Granzyme-2; GranzymeB; granzyme-like protein 1; granzyme-like protein I; GRB; GZB; Gzmb; HLP; human lymphocyte protein; Human lymphocyte protein (Hlp); Lymphocyte protease; Natural killer cell protease 1; OTTHUMP00000028189; RNKP-1; SECT; T-cell serine protease 1-3E